http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
Zhen-Hua Chen,Liang-Peng Sun,Wei Zhang,Qiang Shen,Li-Xin Gao,Jia Li,Hu-Ri Piao 대한화학회 2012 Bulletin of the Korean Chemical Society Vol.33 No.5
Protein tyrosine phosphatase 1B (PTP1B) is a key factor in negative regulation of the insulin pathway, and is a promising target for the treatment of type-II diabetes, obesity and cancer. Herein, compound (4) was first observed to have moderate inhibitory activity against PTP1B with an IC50 value of 13.72 ± 1.53 μM. To obtain more potent PTP1B inhibitors, we synthesized a series of chalcone derivatives using compound (4) as the lead compound. Compound 4l (IC50 = 3.12 ± 0.18 μM) was 4.4-fold more potent than the lead compound 4 (IC50 = 13.72 ± 1.53 μM), and more potent than the positive control, ursolic acid (IC50 = 3.40 ± 0.21 μM). These results may help to provide suitable drug-like lead compounds for the design of inhibitors of PTP1B as well as other PTPs.
Chen, Zhen-Hua,Sun, Liang-Peng,Zhang, Wei,Shen, Qiang,Gao, Li-Xin,Li, Jia,Piao, Hu-Ri Korean Chemical Society 2012 Bulletin of the Korean Chemical Society Vol.33 No.5
Protein tyrosine phosphatase 1B (PTP1B) is a key factor in negative regulation of the insulin pathway, and is a promising target for the treatment of type-II diabetes, obesity and cancer. Herein, compound ($\mathbf{4}$) was first observed to have moderate inhibitory activity against PTP1B with an $IC_{50}$ value of $13.72{\pm}1.53{\mu}M$. To obtain more potent PTP1B inhibitors, we synthesized a series of chalcone derivatives using compound ($\mathbf{4}$) as the lead compound. Compound $\mathbf{4l}$ ($IC_{50}=3.12{\pm}0.18{\mu}M$) was 4.4-fold more potent than the lead compound $\mathbf{4}$ ($IC_{50}=13.72{\pm}1.53{\mu}M$), and more potent than the positive control, ursolic acid ($IC_{50}=3.40{\pm}0.21{\mu}M$). These results may help to provide suitable drug-like lead compounds for the design of inhibitors of PTP1B as well as other PTPs.
Association Between GSTM1 Polymorphism and Nasopharyngeal Cancer Susceptibility: a Meta-analysis
Sun, Zhen-Feng,Zhang, Jia,Xu, Hong-Ming,Wang, Guo-Liang,Dong, Pin Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.11
Background/Aims: Glutathione S-transferase M1 (GSTM1) is a multifunctional enzyme that plays a critical role in the detoxification of varieties of carcinogenic metabolites. Many studies have been conducted to investigate the association between GSTM1 polymorphism and nasopharyngeal cancer (NPC) risk, but the findings among those studies are inconsistent. To assess this relationship more precisely, we performed a meta-analysis of all available studies on the subject. Methods: Case-control studies were identified by searching Pubmed, Embase, ISI Web of Science, and Wanfang databases through September 6, 2012. We used the pooled odds ratio (OR) with its corresponding 95% confidence interval (95%CI) to evaluate the association of GSTM1 polymorphism with NPC susceptibility. Subgroup analyses by pathological types, sex and smoking status were performed to further identify the association. Results: Overall, 11 published studies with 1,513 cases and 2,802 controls were finally included into this meta-analysis according to the inclusion criteria. Meta-analysis of total studies showed that the null genotype of GSTM1 was significantly associated with increased risk of NPC, when comparing with the non-null genotype (OR=1.51, 95%CI=1.33-1.72, POR<0.001). The association was still statistically significant in subgroup analysis of patients with nasopharyngeal squamous cell carcinoma (OR=1.73, 95%CI=1.24-2.42, POR=0.001). Males with the null genotype of GSTM1 were more likely to subject to NPC than females. In addition, the association between the null genotype of GSTM1 and NPC risk was strongest in individuals with exposure to smoking. Sensitivity analysis by sequential omission of any individual studies one at a time further demonstrated the significant association. Conclusions: The findings suggest that the null genotype of GSTM1 is a risk factor for NPC, and there is a gene-smoking interaction in this association.
DNA methylation levels in different tissues in tea plant via an optimized HPLC method
Ying Gao,Jia-Li Hao,Zhen Wang,Kwan-Jeong Song,Jian-Hui Ye,Xin-Qiang Zheng,Yue-Rong Liang,Jian-Liang Lu 한국원예학회 2019 Horticulture, Environment, and Biotechnology Vol.60 No.6
DNA methylation is one of the most important events in epigenetics and significantly influences plant growth and development. In the present study, we established and optimized a high-performance liquid chromatography method for detecting the base composition in DNA in tea plant (Camellia sinensis L.) tissues by using saline buffers-free mobile phases. The DNA methylation level varied with tea plant tissue, cultivar, and growth stage. A relatively higher DNA methylation level was observed in tender leaf (38.34%) and pistil (38.19%) tissues, while a relatively low level was detected in capillary root (19.45%), stamen (19.61%), and old leaf (20.70%) tissues. The pattern of the methylation level formed a saddle curve during the growth of dormant buds in spring; the lowest point appeared at the stage of one leaf and a bud. The methylation level in the adventitious buds regenerated from the branch after pruning seemed to decrease with an increase in the degree of pruning. These DNA methylation levels might be associated with the development of tea plant.
刘振学(Zhen-Xue Liu),崔家强(Jia-Qiang Cui),李明亮(Ming-Liang Li) 대전대학교 환경문제연구소 2010 환경문제연구소 논문집 Vol.14 No.-
The potassium permanganate was used for manganese oxide catalyst with various methods and various processes, which manganese oxide wasused for removal of formaldehyde in flow gas. Experiments indicate that these catalyst materials are difference from each other because of raw materials used and pH values in catalysts preparation when used for formaldehyde removal. And when catalyst was prepared by using potassium permanganate and GLU or PEAunder different pH values respectively, it could have good performance in formaldehyde oxidizing purification. In this thesis, when the ratio of potassium permanganate and GLU was 5:1 for catalyst preparation, and 5 mL of nitric acid was added, the formaldehyde elimination efficiency could be maintained at 100% longer than 600 minutes. And when the ratio of potassium permanganate and PEA was 10:1 for catalyst preparation, and 5 mL of nitric acid was added, the formaldehyde elimination efficiency could be maintained at 100% longer than 705 minutes.
Yan, XiangMin,Wang, Jia,Li, Hongbo,Gao, Liang,Geng, Juan,Ma, Zhen,Liu, Jianming,Zhang, Jinshan,Xie, Penggui,Chen, Lei Asian Australasian Association of Animal Productio 2021 Animal Bioscience Vol.34 No.9
Objective: With the rapid development of proteomics sequencing and RNA sequencing technology, multi-omics analysis has become a current research hotspot. Our previous study indicated that Xinjiang brown cattle have better meat quality than Kazakh cattle. In this study, Xinjiang brown cattle and Kazakh cattle were used as the research objects. Methods: Proteome sequencing and RNA sequencing technology were used to analyze the proteome and transcriptome of the longissimus dorsi muscle of the two breeds of adult steers (n = 3). Results: In this project, 22,677 transcripts and 1,874 proteins were identified through quantitative analysis of the transcriptome and proteome. By comparing the identified transcriptome and proteome, we found that 1,737 genes were identified at both the transcriptome and proteome levels. The results of the study revealed 12 differentially expressed genes and proteins: troponin I1, crystallin alpha B, cysteine, and glycine rich protein 3, phosphotriesterase-related, myosin-binding protein H, glutathione s-transferase mu 3, myosin light chain 3, nidogen 2, dihydropyrimidinase like 2, glutamate-oxaloacetic transaminase 1, receptor accessory protein 5, and aspartoacylase. We performed functional enrichment of these differentially expressed genes and proteins. The Kyoto encyclopedia of genes and genomes results showed that these differentially expressed genes and proteins are enriched in the fatty acid degradation and histidine metabolism signaling pathways. We performed parallel reaction monitoring (PRM) verification of the differentially expressed proteins, and the PRM results were consistent with the sequencing results. Conclusion: Our study provided and identified the differentially expressed genes and proteins. In addition, identifying functional genes and proteins with important breeding value will provide genetic resources and technical support for the breeding and industrialization of new genetically modified beef cattle breeds.
Xiaohong Lu,Haixing Zhang,Zhen-yuan Jia,Yixuan Feng,Steven Y. Liang 대한기계학회 2018 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.32 No.7
Selection of cutting parameters in micro-milling operations is essential for improving machining efficiency and quality, and prolonging the micro-milling tool life. The increase of material removal rate (MRR) always means the increase of cutting parameters, which may lead to poor surface quality and micro-milling tool failure, even cutter breakage. An optimization approach based on genetic algorithm is used to achieve the maximum MRR under the constraints of surface roughness and cutter breakage. A theoretical model for predicting micro-milling cutter breakage is presented and micro-milling experiments were conducted to establish statistical models of cutter breakage and surface roughness. The optimized results were achieved under the constraints of the specified surface roughness and compared under the different surface roughness limitation. We find that the optimized results improve the machining efficiency and quality in micro-milling and is affected by constraint conditions complicatedly.
Xin, Yong,Wang, Jia-Yang,Li, Liang,Tang, Tian-You,Liu, Gui-Hong,Wang, Jian-She,Xu, Yu-Mei,Chen, Yong,Zhang, Long-Zhen Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.3
Objective: To make sure the feasibility with $^{18F}FDG$ PET/CT to guided dynamic intensity-modulated radiation therapy (IMRT) for nasopharyngeal carcinoma patients, by dosimetric verification before treatment. Methods: Chose 11 patients in III~IVA nasopharyngeal carcinoma treated with functional image-guided IMRT and absolute and relative dosimetric verification by Varian 23EX LA, ionization chamber, 2DICA of I'mRT Matrixx and IBA detachable phantom. Drawing outline and making treatment plan were by different imaging techniques (CT and $^{18F}FDG$ PET/CT). The dose distributions of the various regional were realized by SMART. Results: The absolute mean errors of interest area were $2.39%{\pm}0.66$ using 0.6cc ice chamber. Results using DTA method, the average relative dose measurements within our protocol (3%, 3 mm) were 87.64% at 300 MU/min in all filed. Conclusions: Dosimetric verification before IMRT is obligatory and necessary. Ionization chamber and 2DICA of I'mRT Matrixx was the effective dosimetric verification tool for primary focal hyper metabolism in functional image-guided dynamic IMRT for nasopharyngeal carcinoma. Our preliminary evidence indicates that functional image-guided dynamic IMRT is feasible.
Wenjing Cao,Gao Luo,Keying Wang,Chanhua Liang,Wen He,Zhen Zeng,Qi Qi Pang,Jia-Le Song 한국식품영양과학회 2021 한국식품영양과학회 학술대회발표집 Vol.2021 No.10
To observe the effect of SA on lipid metabolism induced by HFD in Syrian hamsters. Changes in body weight(BW) were observed. The serum and hepatic levels of TC, TG, LDL-C, HDL-C and NEFA were determined by ELISA kits. The levels of PPAR-γ, CPT-1, CYP7A1, FAS, ACC1, SREBP2, HMGCR were detected by Western Blotting. Compared with control (CON) group, the weight of hamsters in the HFD group increased, while the weight and gain of hamsters in SA group decreased significantly (P<0.05). The weight of fat was significantly decreased after intervention with SA (P<0.05). The levels of TC, TG, LDL-C and NEFA in serum and liver were increased by HFD (P<0.05); while the serum levels of TC, TG, NEFA and LDL-C in SA group were decreased and HDL-C level was increased. Western-blot results showed that the HFD group’s levels of PPAR-γ, CPT-1 and CYP7A1 were decreased, and the levels of FAS, ACC1, SREBP1, SREBP2 and HMGCR increased; and SA can ameliorates changes in protein levels caused by HFD.Our results suggested that SA reduced fat accumulation, improve lipid metabolism disorder in hamsters induced by HFD.