Mechanical Stretch-Induced Protection against Myocardial Ischemia-Reperfusion Injury Involves AMP-Activated Protein Kinase

Hao, Jia,Kim, Hun-Sik,Choi, Woong,Ha, Tae-Sun,Ahn, Hee-Yul,Kim, Chan-Hyung The Korean Society of Pharmacology 2010 The Korean Journal of Physiology & Pharmacology Vol.14 No.1

AMP-activated protein kinase (AMPK) protects various tissues and cells from ischemic insults and is activated by many stimuli including mechanical stretch. Therefore, this study investigated if the activation of AMPK is involved in stretch-induced cardioprotection (SIC). Intraventricular balloon and aorto-caval shunt (ACS) were used to stretch rat hearts ex vivo and in vivo, respectively. Stretch preconditioning reduced myocardial infarct induced by ischemia-reperfusion (I/R) and improved post-ischemic functional recovery. Phosphorylation of AMPK and its downstream substrate, acetyl-CoA carboxylase (ACC) were increased by mechanical stretch and ACC phosphorylation was completely blocked by the AMPK inhibitor, Compound C. AMPK activator (AICAR) mimicked SIC. Gadolinium, a blocker of stretch-activated ion channels (SACs), inhibited the stretch-induced phosphorylation of AMPK and ACC, whereas diltiazem, a specific L-type calcium channel blocker, did not affect AMPK activation. Furthermore, SIC was abrogated by Compound C and gadolinium. The in vivo stretch induced by ACS increased AMPK activation and reduced myocardial infarct. These findings indicate that stretch preconditioning can induce the cardioprotection against I/R injury, and activation of AMPK plays an important role in SIC, which might be mediated by SACs.

Comparing the Effects of Carvedilol Enantiomers on Regression of Established Cardiac Hypertrophy Induced by Pressure Overload

Jia Hao,김찬형 한국실험동물학회 2010 Laboratory Animal Research Vol.26 No.1

Pressure overload diseases such as valvular stenosis and systemic hypertension morphologically manifest in patients as cardiac concentric hypertrophy. Preventing cardiac remodeling due to increased pressure overload is important to reduce the morbidity and mortality. A recent clinical study has shown that carvedilol has beneficial effects on the survival rate of patients with heart failure. This may be due to the actions of carvedilol such as β-adrenoceptor blockade and the α1-adrenergic receptor blockade effects. Therefore, we investigated whether carvedilol can reverse preexisting cardiac hypertrophy and we compared the effects of racemic carvedilol and the carvedilol enantiomers. Cardiac hypertrophy was induced in rats by suprarenal transverse abdominal aortic constriction (AC). Fifteen weeks after AC surgery, concentric hypertrophy was identified in the AC group by performing echocardiography. Low dose S- and SR-carvedilol (2 mg/kg/day), which were orally administered for three weeks, caused significant regression of the cardiac hypertrophy, and this most significantly occurred in the rats that received S-carvedilol. However, R-carvedilol did not reduce cardiac hypertrophy. Regression of cardiac hypertrophy by carvedilol was confirmed on the echocardiograms and electrocardiograms. These results suggest that carvedilol could reverse the development of leftventricular concentric hypertrophy that is induced by pressure overload. S-carvedilol is proposed to be superior to SR-and R-carvedilol as a beneficial treatment for cardiac hypertrophy.

Research on an Improved Differential Evolution Algorithm based on Three Strategies for Solving Complex Function

Hao Jia 보안공학연구지원센터 2015 International Journal of Smart Home Vol.9 No.11

For the shortcomings of differential evolution algorithm(DE), such as the low convergence rate in the late evolution, easy to trap into the local optimal solution, and weak situation of the global search ability and the stability of optimization, an improved differential evolution algorithm based on multi-population and dynamic local search(MPDLSDE) is proposed in this paper. In the MPDLSDE algorithm, different populations select different mutation operation model in order to obtain superiority reciprocity between different models in the process of evolution. And the random selected method and small probability perturbation are used to increase the diversity of population and balance the exploitation ability and exploration ability of the algorithm. Then dynamic local search method is used to solve the current optimal solution in order to speed up the convergence rate. Several well-known benchmark functions are selected to validate the efficiency of the MPDLSDE algorithm. The simulation experiment and comparative analysis results show that the MPDLSDE algorithm can enhance the global convergence ability and get the high accuracy solution in high dimensional complex optimization problems.

A Novel Hybrid Optimization Algorithm and its Application in Solving Complex Problem

Hao Jia 보안공학연구지원센터 2015 International Journal of Hybrid Information Techno Vol.8 No.2

Ant colony optimization (ACO) algorithm is a new heuristic algorithm which has been demonstrated a successful technology and applied to solving complex optimization problems. But the ACO exists the low solving precision and premature convergence problem, particle swarm optimization (PSO) algorithm is introduced to improve performance of the ACO algorithm. A novel hybrid optimization (HPSACO) algorithm based on combining collaborative strategy, particle swarm optimization and ant colony optimization is proposed for the traveling salesman problems in this paper. The HPSACO algorithm makes use of the exploration capability of the PSO algorithm and stochastic capability of the ACO algorithm. The main idea of the HPSACO algorithm uses the rapidity of the PSO algorithm to obtain a series of initializing optimal solutions for dynamically adjusting the initial pheromone distribution of the ACO algorithm. Then the parallel search ability of the he ACO algorithm are used to obtain the optimal solution of solving problem. Finally, various scale TSP are selected to verify the effectiveness and efficiency of the proposed HPSACO algorithm. The simulation results show that the proposed HPSACO algorithm takes on the better search precision, the faster convergence speed and avoids the stagnation phenomena.

AMP-activated protein kinase mediates the cardioprotective effects of mechanical stretch in ex vivo and in vivo rat hearts

Jia Hao,Hun-Sik Kim,Woong Choi,Chan-Hyung Kim,Hee-Yul Ahn 한국실험동물학회 2008 한국실험동물학회 학술발표대회 논문집 Vol.2008 No.-

AMP-activated protein kinase mediates the cardioprotective effects of mechanical stretch in ex vivo and in vivo rat hearts

Jia Hao,Chan Hyung Kim,Hee-Yul Ahn 한국실험동물학회 2008 한국실험동물학회 학술발표대회 논문집 Vol.2008 No.-

蔡应龙及其“补恨”思想 - 以《紫玉记》为主兼谈《琵琶重光记》

Jia Hao 한국중국언어문화연구회 2021 한중언어문화연구 Vol.- No.59

蔡应龙是清代雍正年间的戏曲作家,他的《紫玉记》是霍小玉故事改编系列中以明代汤显祖《紫箫记》为底本进行增补的“补恨”传奇作品。生活在清早中期的蔡应龙出身于科举世家德清蔡氏,对于“忠孝节义”的遵循内化于他的传奇创作之中。其早期的《琵琶重光记》是对“忠孝”的弥补,稍晚的《紫玉记》则是对“节义”的弥补。同时,由于家难惨遭削籍和长久被疾病所困,不仅影响了他对于“大团圆”结局的审美追求,也影响他对作品中非主要人物的关心。“照应”是蔡应龙区别于其他作家的“补恨”方式,体现了他对于人物性格、情节逻辑以及历史真实的关注。本文从三个方面对蔡应龙及其“补恨”思想进行介绍。第一章介绍从“知人论世”出发,了解蔡应龙的经历及其思想形成的条件。第二章立足《紫玉记》文本本身,分析其内容、结构、删改方式以及最终效果。第三章则从外部环境、作者以及作品与作品关系出发对“补恨”的含义以及“照应”方式进行阐释。

Black rice anthocyanidins prevent retinal photochemical damage via involvement of the AP-1/NF-κB/Caspase-1 pathway in Sprague-Dawley Rats

Hao Jia,Wei Chen,Xiuhua Wu,Shuai Li,Hong Liu,Jiru Liao,Weihua Liu,Mantian Mi,Longjian Liu,Daomei Cheng,Xiaoping Yu 대한수의학회 2013 Journal of Veterinary Science Vol.14 No.3

The effects of black rice anthocyanidins (BRACs) on retinal damage induced by photochemical stress are not well known. In the present study, Sprague-Dawley rats were fed AIN-93M for 1 week, after which 80 rats were randomly divided into two groups and treated with (n = 40) or without BRACs (n =40) for 15 days, respectively. After treatment, both groups were exposed to fluorescent light (3,000 ± 200 lux; 25oC), and the protective effect of dietary BRACs were evaluated afterwards. Our results showed that dietary BRACs effectively prevented retinal photochemical damage and inhibited the retinal cells apoptosis induced by fluorescent light (p < 0.05). Moreover, dietary BRACs inhibited expression of AP-1 (c-fos/c-jun subunits), up-regulated NF-κB (p65) expression and phosphorylation of IκB-α, and decreased Caspase-1 expression (p < 0.05). These results suggest that BRACs improve retinal damage produced by photochemical stress in rats via AP-1/NF-κB/Caspase-1apoptotic mechanisms.

Large-Scale Data Classification Method Based on Machine Learning Model

Hao Jia 보안공학연구지원센터 2015 International Journal of Database Theory and Appli Vol.8 No.2

Classification is to map the data item in the database into a given class. It is an important research direction in data mining. In allusion to the shortcomings of traditional classification methods, such as the decision tree, K nearest neighbor, Bayes , fuzzy logic, genetic algorithms and neural networks and so on, the support vector machine with perfect theory, strong adaptability, global optimization, short training time, good generalization performance is introduced into the classification, a machine learning model based on the SMO algorithm and RBF kernel function of the SVM is proposed to realize a classification method in this paper. This method transforms the nonlinear classification problem into linear classification problem by improving the data dimension. It can better solve the problems of the minimum error in the training set and the larger error in the test set in the traditional algorithm. Application of UCI classification experiment shows that the proposed method takes on the better convergence, faster training speed and higher classification accuracy.

關於漢語中性指示詞“恁”的研究

Jia Hao 한국중국언어문화연구회 2020 한중언어문화연구 Vol.- No.57

This paper studies the modern Chinese demonstrative pronoun "Nen" from three aspects. First of all, on the origin of "Nen", this paper summarizes two kinds of etymologies in the academic circle, namely "Ning, Neng" origin theory which starts from the head consonant letter of "Nen", and another one which starts from the ending “-m” of "nen". Based on these two etymologies, this paper holds that the original origin of "Nen" is "Er" and it is a dialect word influenced by the southern language. Secondly, this paper focuses on the use of "Nen" alone and collates the syntactic functions of the commonly used "Nen" prefixes "Nen Me" and "Nen De". It is concluded that "Nen" can be used as attributive before noun and noun group, adverbial before adjective and verb, also can be used as predicate and object, and also explained the reason why "Nen" is seldom used as subject. Finally, this paper analyzes the phenomenon that "Nen" indicates the uncertainty of distance, and thinks that "Nen" should not be regarded as a neutral demonstractive, but has the function of indicating first, and the distance semantics is based on the specific context and adds the neutral indicator. At the end of the paper, this paper points out that the case that the pronoun "Na" means the near distance and the pronoun "Zhe" means the far distance which is concluded that the deixis "Nen" has a high degree of separation from distance semantics.