Phylogeography of Wolbachia and Its Lytic Phage Shows Incongruent Infection history in Vollenhovia emeryi (Hymenoptera: Myrmicinae)

Soyeon Park,Pureum Noh,Jae Chun Choe,Gilsang Jeong 한국응용곤충학회 2014 한국응용곤충학회 학술대회논문집 Vol.2014 No.04

The phylogenetic analysis the queen polymorphic Vollenhovia emeryi ant reveals derivation of the Wolbachia- free short-winged from the Wolbachia-infected long-winged. However, intriguingly, some Japanese short-winged colonies harbor Wolbachia. Wolbachia specific bacteriophage (WO) is also detected in more than half of the infected colonies with no clear distribution pattern across the host insect lineage. We hypothesized that 1) the infected Japanese short-winged is in the intermediate stage to complete loss of Wolbachia and 2) the phage invaded the host after the host insect diverged. To test the hypotheses, we studied the strain diversity using the multi-locus sequence typing (MLST) of five ant colonies; three longwinged colonies from Korea and one long-winged colony and one short-winged colony from Japan. Both Korean and Japanese V. emeryi colonies show unexpectedly high level of Wolbachia strain diversity. However, the diversity is not significantly different between the long-winged and the short-winged against our first hypothesis. Phylogenies of Wolbachia show Korean strains and Japanese strains are largely monophyletic indicating prior infection before the host divergence. The strain diversity of the phage is also surprisingly high. Phylogenies of orf2 and orf7 genes are incongruent to that of Wolbachia and geographically distinct. This indicates that the phage is spatially static and the current infection pattern may be the consequence of local repeated gain and loss of the phage.

Performance of Two Stage Partial Nitritation and Anammox Porcess Treating High Salt Wastewater

Soyeon JEONG,Jeongmi KIM,Yeonju KIM,Jaecheul YU,Taeho LEE 대한환경공학회 2019 대한환경공학회 학술발표논문집 Vol.2019 No.-

인체 대장암 세포주 SW480에서 docosahexaenoic acid에 의한 침윤억제 기전

Soyeon Shin(신소연),Yong-Jo Kim(김용조),Kyoung-Sub Song(송경섭),Kaipeng Jing,Nayeong Kim(김나영),Soyeon Jeong(정소연),Ji-Hoon Park(박지훈),Kang-Sik Seo(서강식),Jun-Young Heo(허준영),Hyun-Joo Kwon(권현주),Jong-Il Park(박종일),Seung 한국생명과학회 2010 생명과학회지 Vol.20 No.4

대장암은 미국 등 서양 국가뿐만 아니라 국내에서도 2번째로 많이 발병이 되는 암으로 알려져 있다. 역학조사에 의하면 ω3-PUFAs를 많이 섭취한 인종에서 대장암 발생빈도가 감소하고 최근 ω3-PUFAs는 수종의 암에 대해 항암작용을 나타낸다고 한다. 이에 본 연구에서는 대장암에서 DHA 등 ω3-PUFA의 항침윤 기전을 규명하여 다음과 같은 결과를 얻었다. DHA 및 EPA는 대장암 세포주 SW480의 증식을 농도 의존적으로 억제하였으나 AA는 거의 영향이 없었으며 TUNEL assay로 apoptotic cell death가 확인 되었다. DHA는 β-catenin 단백 및 TCF/LEF luciferase 활성을 농도 의존적으로 억제 하였다. SW480 세포의 침윤능은 DHA의 농도에 의존적으로 억제되었다. DHA처리 후 MMP-9 및 MMP-2 mRNA양이 감소되었을 뿐만 아니라 그 promoter의 reporter 활성도 억제되었다. NF-kB 및 p-IkB 단백짙양도 DHA의 처리농도에 의존적으로 감소하였으며 NF-kB promoter의 활성도 억제되었다. 이상의 결과로 ω3-PUFA는 대장암에서 NF-kB 신호전달 차단에 의한 MMP-2 및 MMP-9 발현을 억제하여 침윤을 억제하여 항암작용을 나타낼 수 있음을 시사하며, 따라서 ω3-PUFA는 대장암의 예방 및 치료에 유용하게 사용될 수 있으리라 생각된다. Colon cancer is one of the most common malignancies in the western world and the second leading cause of cancer death in Korea. Epidemiology studies have shown a reduced incidence of colon cancer among populations consuming a large quantity of ω3-polyunsaturated fatty acids (ω3-PUFA) of marine origin. Recently, it has been found that ω3-PUFA has an antineoplastic effect in several cancers. This study was designed to investigate the mechanism of the anti-invasive effect of ω3-PUFA in colon cancer. ω3-PUFA, docosahexaenoic acids (DHA) and eicosapentaenoic acid (EPA) treatment resulted in a dose-dependent inhibition of cell growth in SW480 human colon cancer cells. In contrast, arachidonic acid (AA), a ω6-PUFA, exhibited no significant effect. This action likely involves apoptosis, given that DHA treatment increased apoptotic cells in TUNEL assay. Moreover, invasiveness of SW480 cells was inhibited following treatment of DHA in a dose-dependent manner; in contrast, AA had no effect. The levels of MMP-9 and MMP-2 mRNA decreased after DHA pretreatment. MMP-9 and MMP-2 promoter activities were also inhibited by DHA treatment. The levels of NF-kB and p-IkB protein were down-regulated by DHA pretreatment in a dose dependent manner. In addition, DHA inhibited NF-kB promoter reporter activities. These findings suggest that ω3-PUFA may inhibit cancer cell invasion by inhibition of MMPs via reduction of NF-kB in colon cancer. In conclusion, ω3-PUFA could be used for chemoprevention and treatment of human colon cancer.

Sulfation and Desulfation Behavior of Pt—BaO/MgO—Al₂O₃ NO_x Storage Reduction Catalyst

Jeong, Soyeon,Kim, Do Heui American Scientific Publishers 2016 Journal of Nanoscience and Nanotechnology Vol.16 No.5

<P>The comparative study between Pt-BaO/Al2O3 and Pt-BaO/MgO-Al2O3 gives the information about the effect of MgO addition to Al2O3 support on the sulfation and desulfation behavior of Pt-BaO/MgO-Al2O3 NOx storage reduction catalyst. The sulfated two samples were analyzed by using element analysis (EA), X-ray diffraction (XRD), H-2 temperature programmed reaction (H-2 TPRX) and NOx uptake measurement. The amount of sulfur uptake on 2 wt% Pt-20 wt% BaO/Al2O3 and 2 wt% Pt-20 wt% BaO/MgO-Al2O3 are almost identical as 0.45 and 0.40 of S/Ba, respectively, which yields the drastic decrease in NOx uptake for both sulfated samples. However, after desulfation with H-2 at 600 degrees C, the residual sulfur amount on MgO-Al2O3 supported catalyst is three times larger than that on Al2O3 supported one, indicating that sulfur species formed on the former are more stable than those on the latter. It is also well corresponding to the H-2 TPRX results where the main H2S peak from MgO-Al2O3 supported sample is observed at higher temperature than Al2O3 supported one, resulting in the lower NOx uptake activity of former sample than the latter one. Meanwhile, after desulfation of MgO-Al2O3 supported sample at 700 degrees C and 800 degrees C, the activity is recovered more significantly due to the removal of the large amount of sulfur while Al2O3 supported one decreases monotonically due to the sintering of Pt crystallite and the formation of BaAl2O4 phase. It is summarized that MgO-Al2O3 supported catalyst enhances the thermal stability of the catalyst, however, forms the stable sulfate species, which needs to be improved to develop the more sulfur resistant NSR catalyst system.</P>

Lignin Depolymerization Over CuO–MgO–Al₂O₃ Mixed Oxide Catalysts in Supercritical Ethanol: Effect of Catalyst Preparation Methods

Jeong, Soyeon,Jang, Gyeong Hun,Kim, Do Heui American Scientific Publishers 2017 Nanoscience and Nanotechnology Letters Vol.9 No.2

Cannabidiol-induced apoptosis is mediated by activation of Noxa in human colorectal cancer cells

Jeong, Soyeon,Yun, Hye Kyeong,Jeong, Yoon A,Jo, Min Jee,Kang, Sang Hee,Kim, Jung Lim,Kim, Dae Yeong,Park, Seong Hye,Kim, Bo Ram,Na, Yoo Jin,Lee, Sun Il,Kim, Han Do,Kim, Dae Hyun,Oh, Sang Cheul,Lee, Da Elsevier 2019 Cancer letters Vol.447 No.-

<P><B>Abstract</B></P> <P>Cannabidiol (CBD), one of the compounds present in the marijuana plant, has anti-tumor properties, but its mechanism is not well known. This study aimed to evaluate the apoptotic action of CBD in colorectal cancer (CRC) cells, and focused on its effects on the novel pro-apoptotic Noxa-reactive oxygen species (ROS) signaling pathway. CBD experiments were performed using the CRC cell lines HCT116 and DLD-1. CBD induced apoptosis by regulating many pro- and anti-apoptotic proteins, of which Noxa showed significantly higher expression. To understand the relationship between Noxa and CBD-induced apoptosis, Noxa levels were downregulated using siRNA, and the expression of apoptosis markers decreased. After ROS production was blocked, the level of Noxa also decreased, suggesting that ROS is involved in the regulation of Noxa, which along with ROS is a well-known pro-apoptotic signaling agents. As a result, CBD induced apoptosis in a Noxa-and-ROS-dependent manner. Taken together, the results obtained in this study re-demonstrated the effects of CBD treatment <I>in vivo</I>, thus confirming its role as a novel, reliable anticancer drug.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Our results strongly suggest, for the first time, that CBD can cause Noxa-induced cell death. </LI> <LI> CBD induced apoptotic cell death via ROS/Endoplasmic Reticulum stress-regulated Noxa activation in colorectal cancer cells. </LI> <LI> These results suggest that CBD has important implications for the potential treatment of human CRC. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>

Depolymerization of Protobind lignin to produce monoaromatic compounds over Cu/ZSM-5 catalyst in supercritical ethanol

Jeong, Soyeon,Yang, Seungdo,Kim, Do Heui Elsevier 2017 Molecular catalysis Vol.442 No.-

<P>The depolymerization reaction of Protobind lignin (PL) was conducted over metal supported on ZSM-5 catalysts in supercritical ethanol without supplying additional hydrogen in order to obtain the high yield of monoaromatic compounds. The reaction was performed by changing the various variables of catalyst such as the types of transition metals (Co, Ni and Cu), the amount of metal loading (5 wt.%, 10 wt.% and 30 wt.% of Cu) and the Si/Al-2 ratio of ZSM-5 (30, 50, 80 and 200). Cu/ZSM-5 displayed the highest yield of monoaromatic compounds among the transition metals. When it comes to the Si/Al-2 ratio of CuiZSM-5, 10 wt.% Cu/ZSM-5 with Si/Al-2 of 30 demonstrated the highest yield (98.2 wt.%) of monoaromatic compounds. Moreover, the linear correlation between yield of monoaromatic compound and acid density of CuiZSM-5 was identified. Meanwhile, HSQC NMR analysis provided the evidence about the promotion of the depolymerization of Protobind lignin via hydrogenolysis. In addition, some products such as ethylated compounds were confirmed by HSQC NMR spectra resulting from the alkylation between monoaromatic compounds and ethanol. It was also found that Cu metal plays a promotional role in enhancing the lignin depolymerization. (C) 2017 Elsevier B.V. All rights reserved.</P>

Adhesive protein-based Janus particles for multifunctional drug delivery

Soyeon JEONG,Yeonsu JEONG,Yun Kee JO,Hyung Joon CHA 한국생물공학회 2018 한국생물공학회 학술대회 Vol.2018 No.10

Purification and preliminary analysis of the ATP-dependent unfoldase HslU from the gram-positive bacterium Staphylococcus aureus

Soyeon Jeong,Nam-Chul Ha,Ae-Ran Kwon 한국구조생물학회 2018 Biodesign Vol.6 No.4

The gram-positive bacterium Staphylococcus aureus is a common cause of abscesses, sinusitis and food poisoning. The emergence of antibiotic-resistant strains has caused significant clinical issues worldwide. The HslU-HslV complex was first identified as a prokaryotic homolog of eukaryotic proteasomes. HslU is an unfoldase that mediates the unfolding of the substrate proteins, and it works with the protease HslV in the complex. To date, the protein complex has been mostly studied in gram-negative bacteria. In this study, we report the purification and crystallization of the full-length HslU from S. aureus. The crystal diffracted X-rays to a 3.5 Å resolution, revealing that the crystals belong to space group P2 1 , with unit cell parameters of a = 166.5, b = 189.6, c = 226.6 Å, and β = 108.1°. We solved the phage problem by molecular replacement using the structure of HslU from Haemophilus influenzae as a search model. The cell content analysis with this molecular replacement solution revealed that 24 molecules are contained in the asymmetric unit. This structure provides insight into the structural and mechanistic difference of the HslUV complex of gram-positive bacteria.

High-grade precursor lesions can be used as surrogate markers to identify the epicenter of periampullary carcinomas

Jeong, Byung-Kwan,Sung, You Na,Kim, Sung Joo,An, Soyeon,Park, Hosub,Hwang, Hee Sang,Kang, Hyo Jeong,Lee, Jae Hoon,Song, Ki Byung,Kim, Kyu-pyo,Hwang, Dae Wook,Lee, Sang Soo,Kim, Song Cheol,Hong, Seung- Elsevier 2019 Human pathology Vol.84 No.-

<P><B>Summary</B></P> <P>Identifying the accurate origin of periampullary cancers is important because different origins may trigger different clinicopathological behaviors. The presence of intraepithelial precursor lesions, including high-grade pancreatic intraepithelial neoplasias (PanINs) and/or high-grade biliary intraepithelial neoplasias (BilINs), may be suggestive of the origin of the periampullary carcinoma in challenging cases. To prove the usefulness of high-grade intraepithelial precursor lesions in identifying the origin of ambiguous periampullary cancers, the status and grades of PanINs and BilINs were evaluated in 256 periampullary carcinomas with a well-defined cancer origin as a test set, including 114 pancreatic cancers, 82 distal bile duct cancers, 54 ampullary cancers, and 6 duodenal cancers. One hundred twelve periampullary carcinomas with clinically equivocal epicenter either by radiologic imaging or by endoscopic finding used as a validation set. High-grade PanINs were found more commonly in pancreatic cancers than in distal bile duct, ampullary, and duodenal cancers both in test (<I>P</I> = .002) and validation sets (<I>P</I> < .001). Similarly, high-grade BilINs were identified more frequently in distal bile duct cancers than in ampullary, pancreatic, and duodenal cancers both in test (<I>P</I> < .001) and validation sets (<I>P</I> = .039). High-grade PanINs were found most commonly in pancreatic cancers, whereas high-grade BilINs were seen most frequently in distal bile duct cancers. In addition, both high-grade PanINs and high-grade BilINs are uncommonly noted in ampullary or duodenal cancers. The recognition of high-grade intraepithelial lesions can help identify the primary origin of periampullary cancers, especially when the epicenter of the periampullary cancer is ambiguous.</P> <P><B>Highlights</B></P> <P> <UL> <LI> High-grade PanINs are found most commonly in pancreatic cancers. </LI> <LI> High-grade BilINs are seen most frequently in distal bile duct cancers. </LI> <LI> Recognition of high-grade PanINs or BilINs helps identify origins of periampullary cancers. </LI> </UL> </P>