폐 상피양 세포암에 특이한 Ribonuclease의 작용기전에 관한 연구

이성윤,지행옥,고재경 한양대학교 의과대학 1993 한양의대 학술지 Vol.13 No.1

A neutral ribonuclease (RNase) specific to the epidermoid carcinoma of the lung was isolated from the lung cancer tissue to investigate processes involved in carcinogenesis and suppression of the lung cancer. Also studied were the substrate specificity and mechanism of action of the neutral RNase specific to the lung cancer. Neutral RNase activity in the lung tissue obtained by surgery was unchanged in four varieties of lung cancers (epidermoid carcinoma in 20 cases, 472±1859 umole/g/hr; adenocarcinoma in 5 cases, 5165±1575 umole/g/hr; karge cell carcinoma in 3 cases, 5870±2305 umole/g/hr; small cell carcinoma in 3 cases, 5405±2822 umole/g/hr; control in 31 cases, 4380±1520 umole/g/hr), indicating that RNase assay in the lung tissue could not be used as a biochemical marker for the lung cancer. Neutral RNases in the epidermoid carcinoma tissue of the lung were separated by a DEAE-cellulose column chromatography into 6 peaks, of which the peak Ⅰ neutral RNase isozyme was specific to the epidermoid cancer of the lung. High performance liquid chromatorgraphy (HPLC) and polyacrylamide gel electrophoresis (PAGE) patterns for peak Ⅰ protein from epidermoid carcinoma tissue of the lung appeared to be different from those of control lung tissue. The subpeak Ⅰ-5~8 (in HPLC pattern) that was supposed to be associated with RNase was greatly increased in the lung cancer, indicating that peak Ⅰ protein from epidermoid carcinoma tissue of the lung was specific to the lung cancer and that peak Ⅰ RNase specific to the cancer was located in HPLC subpeak Ⅰ-5~8. The peak Ⅰ neutral RNase was observed to be highly active toward poly C, poly AC and poly ACU and less active toward poly U and RNA, indicating that the peak Ⅰ neutral RNase was the secretory type of RNase. No activity was found toward polydezyribonucleotides and double stranded polyribonucleotides. Majority of products of poly C digest by the peak Ⅰ neutral RNase was analyzed to be oligoribonucleotides, indicating that the RNase was endonuclease in nature. Observations that the peak Ⅰ neutral RNase was specific to the epidermoid carcinoma of the lung and that the enzyme was endonuclease in nature suggested that the RNase might play an important role in process involved in the suppression of the lung cancer.

폐선암조직에서 Neutral Ribonuclease의 분리와 성상에 관한 연구

김응수,고재경,지행옥 한양대학교 의과대학 1993 한양의대 학술지 Vol.13 No.1

Concentrations of nucleic acids and proteins were determined in adenocarcinoma tissue of the lung and were compared with those in the control lung tissue. Also studied were properties of the neutral RNase specific to the lung cancer to investigate the possible role of the RNase in process involved in carcinogenesis of adenocarcinoma of the lung. DNA and protein centents were unchanged, but RNA content was increased in adenocarcinoma tissue of the lung. Neutral RNase activity was unchanged in the cancer tissue, indicating that the RNase could not be used as a marker for the lung cancer. Proteins and neutral RNase in adenocarcinoma tissue of the lung were separated by a DEAE-cellulose column chromatography into 7peaks each, of which the peak I neutral RNase isozyme was not found in the control lung tissue. This indicated that the peak I neutral RNase was specific to the adenocarcinoma of the lung. The peak I neutral RNase isolated from the adenocarcinoma tissue of the lung did not hydrolyze single stranded (ss) polydeoxyribonucleotides and double stranded (ds) polynucleotides, but hydrolyzed ss polyribonucleotides. The enzyme was observed to be highly active toward poly C, poly U and RNA, indicating that the RNase appeared to be mixed type of secretory and nonseretory RNase. The peak I RNase was not active toward A-A and G-G linkages, but unusually highly active toward A-C and A-U linkages (4 to 6 fold as active as C-C linkage). These results indicated that the peak I neutral RNase isolated from the adenocarcinoma of the lung was (1)specific to the lung cancer. (2) mixed type of seretory and nonsecretory enzymes, (3) unusually highly active toward A-C and A-U linkages of ss polyribonucleotides and RNA, suggesting that the RNase might play roles in processes involved in carcinogenesis and suppression of cancer.

Hartman’s 溶液으로 充塡한 血稀釋體外 循環法에 관한 實驗的 硏究

池幸玉(Heng Ok Jee),金共秀(Kong Soo Kim),鄭永煥(Young Whan Jung),尹允鎬(Yoon Ho Yoon),金近鎬(Kun Ho Kim) 대한외과학회 1972 Annals of Surgical Treatment and Research(ASRT) Vol.14 No.6

폐선암에 특이한 Ribonuclease 활성의 조절과 저해에 관한 연구

지행옥,박해문,고재경 한양대학교 의과대학 1996 한양의대 학술지 Vol.16 No.2

Ribonuclease has been known to be involved in processes of carcinogenesis and cancer suppression. The present study was to isolate and purify the RNase isozymes in the adenocarcinoma tissue of lung and to investigate the property of isozyme V activated and exhibiting secretory nature of the enzyme in the cancer tissue. Also studied was effect of RNase inhibitor and polhnucleotides on teh isozyme to investigate how the isozyme was regulated in the cancer tissue. RNase activity was unchanged, but RNase inhibitor activity was significantly increased in the adenocarcinoma tissue of the lung, showing increase in inhibitor/RNase ratio. This indicates that both RNase inhibitor activity and ratio of inhibitor/RNase activity could be used as biochemical markers for the adenocarcinoma of the lung. RNases in the cancer tissue was isolated by a DEAE-cellulose column chromatography into 7 isozymes, of which three isozymes(isozyme I, VI and VII) were not found in the control lung tissue and one isozyme (isozyme V) was greatly increased in the activity. The results indicated that RNase isozymes I, VI and VII were specific to the lung cancer and the isozyme V was activated in the cancer tissue. Activity of RNase inhibitor complexed with the isozyme V activated was increased and ratio of inhibitor/RNase was also increased. The ratio of RNA/poly C for the RNase isozyme V was far less than 1.0 exhibiting strong secretory nature of the enzyme. The RNase isozyme V isolated from the adenocarcinoma tissue of lung was further isolated and purified by HPLC. The purified RNase isozyme V(V-4) was active toward poly C and far less active toward purine polyribonucleotides and RNA. The purified isozyme was highly active toward poly ACU and AC and substrate specificity toward these two heteropolyribonucleotides appeared to be different from that of control lung tissue. The RNase isozyme V (V-4) isolated from the lung cancer tissue was inhibited by nucleic acids and polynucleotides, the degree of inhibition being different from one polynucleotide to other. The results indicated that RNase isozyme V(V-4) was activated inthe adenocarcinoma tissue of the lung, exhibited strong secretory nature of the enzyme and was regulated not only by protein inhibitor, RNase inhibitor but also base sequence of polynucleotides. Substrate specificity for the RNase isozyme V(V-4) appeared to be differetn from that of control lung tissue, suggesting that the isozyme might be specific to the adenocarcinoma of the lung and that the isozyme might play a role in carcinogenesis and cancer suppression of the lung.

폐선암조직의 핵산분해 효소에 대한 연구

지행옥,유정훈,고재경 한양대학교 의과대학 1994 한양의대 학술지 Vol.14 No.1

Activites of cid deoxyribonuclease(DNase), neutral ribonuclease(RNase) and RNase inhibitor were determined in adenocarcinoma tissue of the lung and were compare with those in control tissue of the lung. Also isolated and fractionated were RNases and RNase inhibitors by a DEAE-cellulose column chromatography from the adenocarcinoma tissue of the lung to find out the presence of the enzymes and the enzyme inhibitors specific to the lung cancer. Concentrations of DNA and protein were unchanged, but RNA content was significantly increased(47%) in adenocarcinoma tissue of the lung. Acid DNase activity was significantly increased(66%), but RNase and RNase ingibitor activities were unchanged. The positive rate of acid DNase ativity as a marker for the lung cancer was high, suggesting the acid DNase activity as a biochemical marker for the lung cancer. Neutral RNases in the adenocarcinoma tissue of the lung were separated by a DEAE-cellulose column chromatography into 7 isozymes, of which three(Ⅰ,Ⅵ and Ⅶ) isozymes were found to be specific to the lung cancer. RNase isozymes Ⅵ and Ⅶ were observed to be nonsecretory type of the enzyme and RNase isozyme I was found to be mixed type of the enzyme. The three RNase isozymes specific to the lung cancer exhibited relatively gigh RNase inhibitor activity. Observations that (1)RNase isozyme I was the isozyme specific to the lung cancer exhibiting the mixed type enzyme and that (2)RNase isozyme I exhibited high RNase ingibitor activity suggested that RNase isozyme I might play a role in carcinogenesis and suppression of the lung cancer.

식도암의 외과적 치료

池幸玉 대한의사협회 1996 대한의사협회지 Vol.39 No.1

심장판막질환과 동반된 관상동맥질환의 수술

Reiner, Korfer,Jee, Heng-Ok 대한흉부심장혈관외과학회 1988 Journal of Chest Surgery (J Chest Surg) Vol.21 No.1

Between November, 1984, and May, 1986, 93 patients underwent combined valvular and coronary artery operation. They were 70 male and 23 female, the age ranging from 29 to 82. From this population 89 patients underwent single valve replacement and 4 patients underwent double valve replacement. Patients with mitral valve disease were in the majority present in the age group between 50 till 70, where as in the group after 60 years, patients with aortic valve disease were dominant. The main indication for aortic valve replacement was aortic stenosis and the indication for mitral valve replacement was equal between mitral stenosis and mitral incompetence, the later was due to papillary dysfunction after myocardial infarction. Dyspnea was a very frequent symptom and it was found in nearly all patients. 28 patients had a previous myocardial infarction and severe left ventricular dysfunction. The grafts were placed prior to valve replacement and periods of myocardial ischemia were kept at a minimum by maintaining coronary perfusion throughout the operation. It is our opinion that simultaneous valve replacement and myocardial revascularization does not increase the risk of cardiac valve replacement substantially.

폐 편평세포암 조직에서 RNase와 RNase 억제물질과의 상호작용에 관한 연구

김형준,지행옥,고재경 한양대학교 의과대학 1996 한양의대 학술지 Vol.16 No.1

This study was performed in 20 cases of squamous carcinoma of lung and 20 cases of control group. RNase activity was unchanged, but RNase inhibitor activity was increased significantly in the squamous cell carcinoma tissue of the lung. Proteins in both lung cancer tissue and control lung tissue were isolated by a DEAE-cellulose column chromatography into 8 peaks, of which 5 peak proteins for the lung cancer tissue and 4 peak proteins for the control lung tissue exhibied a significant activity of RNase. RNAse activity was markedly increased and RNase inhibitor activity was also increased, RNA/poly C ratio for the RNase activity being higher in the RNase isozyme Ⅰ fraction isolated in the lung cancer tissue. A considerable increase in the activity of the RNase isozyme Ⅰ specific to the squamous cell carcinoma of the lung was observed folowing the pretreatmet of the lung cancer tissue extract with parahydroxymercurybenzoate(PHMB), showing higher RNA/poly C ratio for the RNase activity. This means that the RNase released from the RNase-inhibitor complex (the inhibitor free RNase) was found mostly inthe RNase isoyzme Ⅰ and that the inhibitor free RNase was more likely nonsecretory type RNase than the free RNase. The inhibitor free RNase isozyme Ⅰ isolated from the squanous cell carcinoma tissue of the lung was highly active toward poly C, AC and AU, the activity being decreased toward poly ACU, CIU, CI, CU, U, RNA, AGU in order. Nearly no activity was observed toward poly AG, GU and ACG. Substrate spectificity of the free RNase isozyme Ⅰ from the lung cancer tissue was similar in pattern to the of the inhibitor free RNase isozyme Ⅰ, the activity toward poly U, RNA, poly CU, AG, CIU and ACU was lower in the free RNase isozyme Ⅰ. Observations that (1) RNase activity was unchanged, but RNAse inhibitor activity was increased in the squamous cell carcinoma tissue of lung, (2) activities of free RNase and inhibitor free RNase in the RNase isozyme Ⅰ fraction isolated from the lung cancer tissue was markedly increased, (3) the pretreatment of the lung cancer tissue extract with PHMB increased the activity of inhibitor free RNase isozyme Ⅰ and its RNA/poly C ratio, (4) substrate specifricity for the inhibitor free RNase isozyme Ⅰ appeared to be different from that of the free RNase isozyme Ⅰ in the lung cancer tissue suggested that the inhibitor free RNase isozyme Ⅰ isolated from the squamous cell carcinoma tissue was specific to the lung cancer, could be regulated by RNase inhibitor and played an important role in RNA mediated tumorigenesis of the lung cancer.

종격동 종양 및 낭종에 대한 임상적 고찰

김혁,지행옥,Kim, Hyuck,Jee, Heng-Ok 대한흉부심장혈관외과학회 1988 Journal of Chest Surgery (J Chest Surg) Vol.21 No.4

Fifty-five patients with primary mediastinal tumors and cysts which were seen at T.S. Dept., HYUH, were analyzed clinically, histologically, in an incidence of anatomic location and therapeutic results. The results were summarized as follows; 1] The ages of the patients in this series ranged from 15 months to 79 years with the highest incidence in the age group of third decade, and there were no sex distribution[M:F=0.96]. 2] The most frequently encountered tumors were teratodermoid tumors[29%] followed by neurogenic tumors[22%], thymomas[15%] and benign cysts[11%] in decreasing order of frequency. 3] Based on the subdivision of the mediastinum, 62% of the tumors were in the antero-superior mediastinum, 7% in the middle mediastinum and 31% in the posterior mediastinum. 4] The most frequent symptom was chest pain and others were dyspnea, cough, chest tightness and dysphagia. Asymptomatic patients were 29%. 5] Benign tumors and cysts were 71% and malignant tumors were 29%. 6] The successful removal was possible in all the benign mediastinal tumors and cysts[39 cases] and partial removal or biopsy was performed in the 12 cases among 16 cases of malignant mediastinal tumors. 7] Postoperative complications were bleeding, chylothorax, vocal cord paralysis, wound infection and hypothyroidism. 8] The most frequent mediastinal tumor in the West is neurogenic tumor but is teratoma in Korea.

폐선암에 특이한 Ribonuclease Isozyme의 성상에 관한 연구

정종수,지행옥,고재경 한양대학교 의과대학 1995 한양의대 학술지 Vol.15 No.2

It has been reported that ribonucleases (RNases) are involed in carcinogenesis processes and play variable roles in metabolic events occuring cancer tissues. The present study was to investigate the mechanism of action of the RNase activated in and specific to adenocarcinoma of the lung. RNase isozyme V appeared to be specific to the adenocarcinoma was separated and purified by a DEAE cellulose column chromatography and high performance liquid chromatography (HPLC) and was analyzed for substrate specificity and mode of action. RNases in the adenocarcinoma tissue of the lung were separated by a DEAE cellulose column chromatography into 7 isozymes, of which four isozymes including RNase isozyme V activated and three isozymes specific to the cancer. The RNase isozyme V was further purified by 1200 folds by a HPLC. The purified RNase isozyme V was highly active toward poly ACU, AC, A and CU and less active toward RNA, poly A and U, indicating that the isozyme was secretory type of RNase. No activity was observed toward poly G, AG, GU, ACG and AGU. The substrate specificity for the RNase isozyme V in the lung cancer tissue appeared to be different from that in the control lung tissue, suggesting that the isozyme in the lung cancer tissue might be specific to the adenocarcinoma of the lung. Majority of hydrolytic products of poly C by the action of the RNase isozyme V from the lung cancer tissue was observed to be oligo-or poly-ribonucleotides with chain length between 5 and 20, indicating the isozyme as an endonuclease in nature. The RNase isozyme V isolated and purified from the adenocarcinoma tissue of the lung is activiated in and specific to the lung cancer, is the secretory type of RNase, is highly active toward poly A, AC, ACU and CU and is an endoribonclease in nature. The results suggest the possible role of the RNase isozyme V in the suppressive action of carcinogenesis processes in adenocarcinoma of the lung.