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Sethi Benu,Jain Monika,Chowdhary Manish,Soni Yogesh,Bhatia Yukti,Sahai Vikram,Mishra Saroj The Korean Society for Biotechnology and Bioengine 2002 Biotechnology and Bioprocess Engineering Vol.7 No.1
The cloning and expression of $\beta-glucosidase$ II, encoded by the gene ${\beta}glu2$, from thermotolerant yeast Pichia etchellsii into Escherichia coli is described. Cloning of the 7.3 kb BamHI/SalI yeast insert containing ${\beta}glu2$ in pUC18, which allowed for reverse orientation of the insert, resulted in better enzyme expression. Transformation of this plasmid into E. coli JM109 resulted in accumulation of the enzyme in periplasmic space. At $50^{\circ}C$, the highest hydrolytic activity of 1686 IU/g protein was obtained on sophorose. Batch and fed-batch techniques were employed for enzyme production in a 14 L bioreactor. Exponential feeding rates were determined from mass balance equations and these were employed to control specific growth rate and in turn maximize cell growth and enzyme production. Media optimization coupled with this strategy resulted in increased enzyme units of 1.2 kU/L at a stabilized growth rate of $0.14\;h^{-l}$. Increased enzyme production in bioreactor was accompanied by formation of inclusion bodies.
Reliability and Validity of the Diet Quality Index for 7–9-year-old Indian Children
Ritushri Chamoli,Monika Jain,Gargi Tyagi 대한소아소화기영양학회 2019 Pediatric gastroenterology, hepatology & nutrition Vol.22 No.6
Purpose: This study verified the reliability and validity of the Diet Quality Index for Indian children (DQIIC). Methods: The study sample included 100 school going boys and girls (7–9 years). The dietary behavior of children was studied using a questionnaire and 24-hour dietary recall. The DQIIC comprises 21 items which aimed to assess the diet of Indian children on the basis of variety, adequacy of consumption of dietary components, and moderation in the amount of nutrients that are associated with diseases and dietary habits. The reliability of the DQIIC was assessed using the split half method, Cronbach's alpha, and test retest reliability. Content validity was evaluated using content validity ratio (CVR). Results: The intake of iron was found to be less than the recommended dietary allowances. There was frequent consumption of empty calorie packaged foods and higher than the recommended amount of sodium. Reliability of the DQIIC was 0.85 by split half method and the correlation coefficient for test retest reliability was 0.87. Cronbach's alpha was 0.62 and CVR was 0.85 for this index. Most of the children fell in the moderately healthy category. Conclusion: The DQIIC is a reliable and valid tool to assess the diet quality of Indian children between 7–9 years.
Reliability and Validity of the Diet Quality Index for 7-9-year-old Indian Children
Chamoli, Ritushri,Jain, Monika,Tyagi, Gargi The Korean Society of Pediatric Gastroenterology 2019 Pediatric gastroenterology, hepatology & nutrition Vol.22 No.6
Purpose: This study verified the reliability and validity of the Diet Quality Index for Indian children (DQIIC). Methods: The study sample included 100 school going boys and girls (7-9 years). The dietary behavior of children was studied using a questionnaire and 24-hour dietary recall. The DQIIC comprises 21 items which aimed to assess the diet of Indian children on the basis of variety, adequacy of consumption of dietary components, and moderation in the amount of nutrients that are associated with diseases and dietary habits. The reliability of the DQIIC was assessed using the split half method, Cronbach's alpha, and test retest reliability. Content validity was evaluated using content validity ratio (CVR). Results: The intake of iron was found to be less than the recommended dietary allowances. There was frequent consumption of empty calorie packaged foods and higher than the recommended amount of sodium. Reliability of the DQIIC was 0.85 by split half method and the correlation coefficient for test retest reliability was 0.87. Cronbach's alpha was 0.62 and CVR was 0.85 for this index. Most of the children fell in the moderately healthy category. Conclusion: The DQIIC is a reliable and valid tool to assess the diet quality of Indian children between 7-9 years.
Saroj Mishra,Benu Sethi,Monika Jain,Manish Chowdhary,Yogesh Soni,Yukti Bhatia,Vikram Sahai 한국생물공학회 2002 Biotechnology and Bioprocess Engineering Vol.7 No.1
The cloning and expression of β-glucosidase II, encoded by the gene bglu2, from thermo- tolerant yeast Pichia etchellsii into Escherichia coli is described. Cloning of the 7.3 kb BamHI/SalI yeast insert containing bglu2 in pUC18, which allowed for reverse orientation of the insert, resulted in better enzyme expression. Transformation of this plasmid into E. coli JM109 resulted in accumulation of the enzyme in periplasmic space. At 50oC, the highest hydrolytic activity of 1686 IU/g protein was obtained on sophorose. Batch and fed-batch techniques were employed for enzyme production in a 14 L bioreactor. Exponential feeding rates were determined from mass balance equations and these were employed to control specific growth rate and in turn maximize cell growth and enzyme production. Media optimization coupled with this strategy resulted in increased enzyme units of 1.2 kU/L at a stabilized growth rate of 0.14 h-1. Increased enzyme production in bioreactor was accompanied by formation of inclusion bodies.
Pradeep Kumar Siddappa,Neha Jain,Naveen K. Agarwal,Monika Jain,Gurwant Singh Lamba 대한소화기내시경학회 2020 Clinical Endoscopy Vol.53 No.5
Background/Aims: Diagnostic abdominal paracentesis has been described in literature to have variable sensitivity of 50%–75% forthe detection of peritoneal carcinomatosis (PC). We believe that random needle aspirates from the omentum, even in the absence ofobvious deposits by endoscopic ultrasound (EUS), could prove malignancy in patients with PC. Methods: Consecutive patients who underwent EUS for diagnosis and staging of cancer and found to have ascites were includedafter obtaining informed consent. EUS-guided fine needle aspiration (EUS-FNA) from random sites in the omentum was performedthrough the transgastric route using a linear echoendoscope. Results: Fifty-four patients underwent EUS during October 2015 to April 2017 for detection, staging, or FNA of a suspectedmalignant lesion. Ascites was seen in 17 patients and 15 patients who fulfilled the criteria were included. The procedure wassuccessful in all patients. Cytology was suggestive of malignancy in 12 (80%) but not suggestive of malignancy in 3 (20%) patients. Three patients who tested negative had hyperbilirubinemia with biliary obstruction. Their ascitic fluid analysis result was alsonegative. Conclusions: Random FNA of the omentum in patients with malignancy-related ascites is highly effective in the diagnosis of PC andcould be employed during EUS evaluation of malignancies.