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Thanh-Sang Vo,김정애,Isuru Wijesekara,공창숙,김세권 한국식품과학회 2011 Food Science and Biotechnology Vol.20 No.5
The effect of solvent fractions from brown algae (Ishige okamurae) on allergic inflammatory reactions was investigated via measuring histamine release and cytokine generation in human basophilic KU812F cells induced by calcium ionophore A23187. Moreover, polyunsaturated fatty acids (PUFAs) profiles were identied and quantied in the most active fraction. It was found that n-hexane and ethyl acetate (EtOAc) fraction at 50 μg/mL significantly reduce histamine release with the release rates of 24 and 20%, respectively. Likewise, the suppressive effect of nhexane and EtOAc fraction on production as well as expression of interleukin (IL)-4 and IL-13 was determined. Notably, EtOAc fraction exhibited more effective inhibition than n-hexane in all assays. Furthermore, GC analysis revealed that EtOAc fraction contains various antiinflammatory PUFAs such as eicosapentaenoic acid,eicosatrienoic acid, linolenic acid, and γ-linolenic acid. Collectively, EtOAc fraction from brown algae could be a potential inhibitor of allergic inflammatory reactions in basophils that is suggested to be due to PUFAs.
Original Articles : In Vitro and Intracellular Antioxidant Activities of Brown Alga Eisenia bicyclis
( Na Young Yoon ),( Sang Hoon Lee ),( Isuru Wijesekara ),( Se Kwon Kim ) 한국수산과학회(구 한국수산학회) 2011 Fisheries and Aquatic Sciences Vol.14 No.3
The antioxidant activities of a methanolic extract of Eisenia bicyclis and its organic solvent fractions, including dichloromethane (CH2Cl2), ethyl acetate (EtOAc), n-butanol (n-BuOH), and water (H2O) fractions, were investigated. Scavenging activities against DPPH, hydroxyl, superoxide anion, and peroxynitrite radicals were evaluated using electron spin resonance spectrometry; intracellular reactive oxygen species (ROS) were evaluated by a 2′,7′-dichlorofluorescein diacetate assay using RAW264.7 mouse macrophages. The antioxidant activities of the individual fractions were: EtOAc>n-BuOH>CH2Cl2>H2O. The EtOAc fraction exhibited strong radical scavenging activity and a significantly reduced ROS level in RAW264.7 cells. Moreover, the phenolic contents of the extract and fractions followed the same order as their radical scavenging activities. Our results indicate that E. bicyclis is a valuable natural source of antioxidants that may be applicable to the functional food industry.
In Vitro and Intracellular Antioxidant Activities of Brown Alga Eisenia bicyclis
Yoon, Na-Young,Lee, Sang-Hoon,Wijesekara, Isuru,Kim, Se-Kwon The Korean Society of Fisheries and Aquatic Scienc 2011 Fisheries and Aquatic Sciences Vol.14 No.3
The antioxidant activities of a methanolic extract of Eisenia bicyclis and its organic solvent fractions, including dichloromethane ($CH_2Cl_2$), ethyl acetate (EtOAc), n-butanol (n-BuOH), and water ($H_2O$) fractions, were investigated. Scavenging activities against DPPH, hydroxyl, superoxide anion, and peroxynitrite radicals were evaluated using electron spin resonance spectrometry; intracellular reactive oxygen species (ROS) were evaluated by a 2',7'-dichlorofluorescein diacetate assay using RAW264.7 mouse macrophages. The antioxidant activities of the individual fractions were: EtOAc>n-BuOH>$CH_2Cl_2$ >$H_2O$. The EtOAc fraction exhibited strong radical scavenging activity and a significantly reduced ROS level in RAW264.7 cells. Moreover, the phenolic contents of the extract and fractions followed the same order as their radical scavenging activities. Our results indicate that E. bicyclis is a valuable natural source of antioxidants that may be applicable to the functional food industry.