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Sun-JungCho,JaeSeobJung,SeungChulShin,IngNyolJin,BokHyunKo,YunheeKimKwon,HaeyoungSuh-Kim,문일수 생화학분자생물학회 2003 Experimental and molecular medicine Vol.35 No.6
The 2',3'-cyclic nucleotide 3'-phosphodiesterase (CNP), a protein of unknown function in vivo, is abundantly expresed in myelinating glia in two isoforms, CNP1 and CNP2. In this study, imu-noblot analysis showed that CNP1 is the major isoform in adult forebrain, and that both isoforms are included in the postsynaptic density (PSD) fraction and tyrosine-phosphorylated at the basal level. However, subcellular distribution and deter-gent extraction data showed that CNP is nonspeci-ficaly associated with the PSD fraction. Imuno-cytochemistry revealed that CNP is detected, in a weak but punctate patern, in disociated rat hipo-campal neurons of 3 days to 2 weeks in vitro. The CNP-positive punctae were distributed throughout soma and dendrites, and distinct from PSD95-posi-tive ones. Imunoblot analysis indicated that CNP and F11. Interestingly, in addition to the known two isoforms, a new CNP isoform of MW 45 kDa was expresed in these cel lines and was the ma-jor type of isoform in F1 cells. Taken together, our data sugest that CNP is expressed in the ear-ly stage of in vitro development and nonspeci-ficaly included in the adult rat PSD fraction.
Saccharomyces cerevisiae KNU5377 Stress Response during High-Temperature Ethanol Fermentation
김일섭,윤호성,김영생,김현,INGNYOLJIN 한국분자세포생물학회 2013 Molecules and cells Vol.35 No.3
Fuel ethanol production is far more costly to produce than fossil fuels. There are a number of approaches to cost-effective fuel ethanol production from biomass. We characterized stress response of thermotolerant Saccharomyces cerevisiae KNU5377 during glucose-based batch fermentation at high temperature (40°C). S. cerevisiae KNU5377 (KNU5377) transcription factors (Hsf1, Msn2/4, and Yap1), metabolic enzymes (hexokinase, glyceralde-hyde-3-phos-phate dehydrogenase, glucose-6-phosphate dehydrogenase, isocitrate dehydrogenase, and alcohol dehydrogenase), antioxidant enzymes (thioredoxin 3, thioredoxin reductase, and porin), and molecular chaperones and its cofactors (Hsp104, Hsp82, Hsp60, Hsp42, Hsp30, Hsp26, Cpr1, Sti1, and Zpr1) are upregulated during fermentation, in comparison to S. cerevisiae S288C (S288C). Expression of glyceraldehyde-3-phosphate dehydrogenase increased significantly in KNU5377 cells. In addition, cellular hydroperoxide and protein oxidation, particularly lipid peroxidation of triosephosphate isomerase, was lower in KNU5377 than in S288C. Thus, KNU5377 activates various cell rescue proteins through transcription activators, improving tolerance and increasing alcohol yield by rapidly responding to fermentation stress through redox homeostasis and proteostasis.