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외모에 대한 사회문화적 태도의 내면화, 외모도구성과 신체이형장애 성향의 관계: 자기대상화의 매개효과와 자기개념의 다면성의 조절효과
윤혜민 ( Hyemin Yoon ),이영호 ( Youngho Lee ) 사단법인 아시아문화학술원 2020 인문사회 21 Vol.11 No.6
본 연구는 외모에 대한 사회문화적 태도 내면화와 외모도구성이 신체이형장애 성향에 미치는 영향과 자기대상화의 매개효과를 검증하고, 자기대상화가 신체이형장애 성향에 미치는 영향이 자기개념 다면성에 의해 조절되는지를 알아보았다. 대학(원)생 443명을 대상으로 외모에 대한 사회문화적 태도-내면화척도(SATAQ-I), 외모도구성 척도(BVI-SI-A), 객체화 신체의식 척도(K-OBCS), 다면적 자기개념 검사(MSCI), 신체상 걱정 검사(BICI)를 실시하였다. 연구 결과, 외모에 대한 사회문화적 태도 내면화, 외모도 구성, 자기대상화, 신체이형장애 성향 간 정적 상관이, 자기개념 다면성과 그 외 모든 변인 간 부적 상관이 나타났다. 외모에 대한 사회문화적 태도 내면화와 외모도구성은 자기대상화를 매개로 신체이형장애 성향에 영향을 미쳤다. 자기개념에서 외모 이외 영역의 중요도 비중이 자기대상화의 신체이형장애 성향에 대한 영향을 조절할 것이라는 가설은 지지되지 않았으나, 사회적 자기의 중요도 비중이 외모에 대한 사회문화적 태도 내면화가 자기대상화에 미치는 영향을 조절하였다. The present study aimed to verify the effects of internalized sociocultural attitudes toward appearance (ISATA) and social instrumentality of appearance (SIA) on body dysmorphic (BD) trait and examined whether self-objectification (SO) mediates the relation between ISATA and BD, and between SIA and BD. In addition, this study tested whether multiphasic self-concept (MSC) moderates the mediation effects above. Self-report questionnaires, SATAQ-I (sociocultural attitudes toward appearance questionnaireinternalization), BVI-SI-A (body value inventory-social instrumentality- appearance), K-OBCS (Korean objectified body consciousness scales), a shortened form of MSCI (multiphasic self-concept inventory), BICI (body image concern index) were conducted on 443 (under)graduate students. The result were as follows. SO appeared to have mediation effect between ISATA and BD, and between SIA and BD. MSC score did not moderate the effect of SO on BD symptoms. In the additional examination, the ratio of social self in importance was found to moderate the effect of ISATA on SO.
A-Ram Kim,Hyemin Lim,Hyun-So Ji,Ung-Han Yoon,Tae-Ho Kim,Gang-Seob Lee 한국육종학회 2014 한국육종학회 심포지엄 Vol.2014 No.07
Crops are exposed to various environmental stresses. These have been affecting the growth of crops, resulting in the severe loss of agronomic production in many countries. Therefore, development of new varieties of resistant crops is required to assure the desired productivity of crops in stress conditions. In this study, a putatively stress-related gene BrTSR53 was isolated from Brassica rapa. The BrTSR53 is 481 bp long and contains ORF region of 234 bp. The expression of BrTSR53 was determined by quantitative real-time PCR analysis. After 3 hr, the highest quantities of mRNA were revealed in cold and salt stress treatments. In drought stress treatments, there was the highest expression after 36 hr. Therefore, it was confirmed that the ORF in BrTSR53 should be a gene that confer increased resistance to B. rapa growing in different stress conditions. The ORF region of BrTSR53 gene was cloned into an expression vector, pYES-DEST52, and a new protein with molecular weight of 13 kDa was detected by western blot analysis. Also, stress tolerance tests showed that BrTSR53-ORF transgenic yeast exhibited increased resistance to the salt stresses compared with the control. In conclusion, the present data predicts that novel ORF in BrTSR53 can serve as an important genetic resource for abiotic stress resistance.
Youngchul Yoo,Jong Chan Park,Joo-Mi Yoon,Hyemin Lim,Gang-Seob Lee,Sang-Won Lee 한국육종학회 2013 한국육종학회 심포지엄 Vol.2013 No.07
Plants have evolved a set of protecting mechanisms against pathogens, which include secondary metabolites and induced defense responses to pathogen attack. The biological role of purine alkaloids including caffeine is largely unknown. It has been proposed that caffeine confers a resistance against pathogenic bacteria and herbivores. We, in this study, tested direct effects on the growth of rice pathogenic microbes, Xanthomonas oryzae pv. oryzae (Xoo) causing a bacterial leaf blight and Magnaporthe grisea (M. grisea) causing a rice blast. Cell growth of Xoo and M. grisea were significantly retarded in presence of high concentration (2mM) of caffeine. Exogenous caffeine (5mM) induced resistance of wild type rice (cv. Dongjin, susceptible to Xoo and M. grisea) against those pathogens. These results indicated that caffeine enhanced the basal resistance to infection with Xoo. In addition, expression of pathogenesis-related (PR) genes was tested in the caffeine treated rice to elucidate the acquired resistance by caffeine, resulted in induction of PR genes including OsPR1a and OsPrb1. We have generated a transgenic rice producing caffeine by introduction of three N- methyltransferase genes (CaXMT1, CaMXMT1, CaDXMT1) identified from coffee plant. The transgenic rice successfully expressed the three genes, synthesized caffeine up to 5ug/g and showed enhanced resistance to Xoo. We also observed that transcripts of PR genes such as the OsPR1a and OsPrb1 encoding PR-1 type pathogenesis-related protein increased in the caffeine-producing rice. These result showed that caffeine is likely to act a powerful factor to increase level of rice defense as a natural and non-harmful metabolite.
Molecular analysis of T-DNA insertion mutant population for transgenic rice plants
Hyemin Lim,A-Ram Kim,Jung-Il Cho,Hyeon-So Ji,Ung-Han Yoon,Tae-Ho Kim,Gang-Seob Lee 한국육종학회 2014 한국육종학회 심포지엄 Vol.2014 No.07
We have generated 383 independent transgenic lines for genetically modified (GM) rice that contained GPD, UtrCSP, BrTSR15 and BrTSR53 genes overexpression constructs under the control of the constitutive CaMV 35S promoter. TaqMan copy number assay was determined inserted T-DNA copy number. Also FSTs analysis was isolated from 203 single copy T-DNA lines of transgenic plants and sequence mapped to the rice chromosomes. In analyzing single copy lines, we identified 95 FSTs, among which 37 (38.9%) were integrated into genic regions and 58 (61.1%) into intergenic regions. About 27 homozygous lines were obtained through multi-generations of planting, resistance screening and TaqMan copy number assay. To investigate the transgene expression patterns, quantitative real-time PCR analysis was performed using total RNAs from leaf tissue of single copy, intergenic region of T-DNA insertion and homozygous T2 plants. The mRNA expression levels of the examined transgenic rice were significantly increased in all of the transgenic plants. In addition, myc-tagged 35S::BrTSR15 and 35S::BrTSR53 transgenic plants were displayed higher levels of transgene protein than WT plants. These results may be useful for producing of large-scale transgenic plants or T-DNA inserted mutants in rice.
Hyemin Lim,Sung Han Park,A-Ram Kim,Hyeon-So Ji,Ung-Han Yoon,Tae-Ho Kim,Soo-Chul Park,Jang-Ho Hahn,Gang-Seob Lee 한국육종학회 2012 한국육종학회 심포지엄 Vol.2012 No.07
In plants, the Dof (DNA binding with One Finger) proteins are plant-specific transcription factors with a particular class of zinc-finger DNA-binding domain. The Dof genes have been predicted 30 different Dof genes in the rice Oryza sativa genome by phylogenetic analysis. The mostly Dof proteins contain a conserved region of 50 amino acids with a C2-C2 zinc finger motifs that binds a cis-regulatory element sequence 5’-T/AAAAG-3’. We found that a member of the DOF transcription factor family, Dof1 gene of rice, was expressed to wound from Ds insertion mutant population. Sequencing of the flanking regions of the transposon insertion site indicated that the gene-trap had been inserted near the front of the second exon of OsDof1 gene in chromosome 7. Genomic southern analysis revealed that mutant line contained a single copy of Ds gene trap. The Ds tagged rice mutant line, OsDof1::Ds, wound-inducible GUS expression was identified. To analyze the cis-acting elements, we constructed fusion genes with the OsDof1 promoter fused to the β-glucuronidase (GUS) reporter gene and transformed Arabidopsis and rice plants with these constructs. Wound-induced GUS expression was observed in the leaves of transgenic OsDof1::GUS rice and Arabidospsis plants. These results showed that, OsDof1 protein might be involved in stress responses and growth regulation in plant, might plays a role as a transcription regulator in stress response signal transduction pathways of plant.
Renal infarction caused by paradoxical embolism through a patent foramen ovale
( Hyemin Jeong ),( Hyun Woo Lee ),( Ji Young Joung ),( Yoon Young Cho ),( Dongmo Je ),( Kyungmin Huh ),( Hye Ryoun Jang ),( Wooseong Huh ) 대한신장학회 2012 Kidney Research and Clinical Practice Vol.31 No.3
A 48-year-old man presented with acute right flank pain. A computed tomography scan revealed right renal infarction. Because he had no thrombosis in the renal vessels and no clear embolic source, a further examination was performed to find the cause of the renal infarction. On transesophageal echocardiography, a right- to-left shunt during the Valsalva maneuver established a diagnosis of patent foramen ovale. This is a case of paradoxical embolism through a PFO leading to renal infarction.