Construction of a New Agrobacterium tumefaciens-Mediated Transformation System based on a Dual Auxotrophic Approach in Cordyceps militaris

Huan huan Yan,Yi tong Shang,Li hong Wang,Xue qin Tian,Van-Tuan Tran,Li hua Yao,Bin Zeng,Zhi hong Hu The Korean Society for Microbiology and Biotechnol 2024 Journal of microbiology and biotechnology Vol.34 No.5

Cordyceps militaris is a significant edible fungus that produces a variety of bioactive compounds. We have previously established a uridine/uracil auxotrophic mutant and a corresponding Agrobacterium tumefaciens-mediated transformation (ATMT) system for genetic characterization in C. militaris using pyrG as a screening marker. In this study, we constructed an ATMT system based on a dual pyrG and hisB auxotrophic mutant of C. militaris. Using the uridine/uracil auxotrophic mutant as the background and pyrG as a selection marker, the hisB gene encoding imidazole glycerophosphate dehydratase, required for histidine biosynthesis, was knocked out by homologous recombination to construct a histidine auxotrophic C. militaris mutant. Then, pyrG in the histidine auxotrophic mutant was deleted to construct a ΔpyrG ΔhisB dual auxotrophic mutant. Further, we established an ATMT transformation system based on the dual auxotrophic C. militaris by using GFP and DsRed as reporter genes. Finally, to demonstrate the application of this dual transformation system for studies of gene function, knock out and complementation of the photoreceptor gene CmWC-1 in the dual auxotrophic C. militaris were performed. The newly constructed ATMT system with histidine and uridine/uracil auxotrophic markers provides a promising tool for genetic modifications in the medicinal fungus C. militaris.

Thyroid-Associated Orbitopathy: Evaluating Microstructural Changes of Extraocular Muscles and Optic Nerves Using Readout-Segmented Echo-Planar Imaging-Based Diffusion Tensor Imaging

Huan-Huan Chen,Hao Hu,Wen Chen,Dai Cui,Xiao-Quan Xu,Fei-Yun Wu,Tao Yang 대한영상의학회 2020 Korean Journal of Radiology Vol.21 No.3

Objective: We aimed to investigate the ability of readout-segmented echo-planar imaging (rs-EPI)-based diffusion tensor imaging (DTI) in assessing the microstructural change of extraocular muscles (EOMs) and optic nerves in patients with thyroidassociated orbitopathy (TAO) as well as in evaluating disease activity. Materials and Methods: We enrolled 35 TAO patients and 22 healthy controls (HCs) who underwent pre-treatment rs-EPIbased DTI. Mean, axial, and radial diffusivity (MD, AD, and RD) and fractional anisotropy (FA) of the medial and lateral EOMs and optic nerve for each orbit were calculated and compared between TAO and HC groups and between active and inactive TAO groups. Factors such as age, sex, disease duration, mediation, and smoking history between groups were also compared. Logistic regression analysis was used to evaluate the predictive value of significant variables for disease activity. Results: Disease duration was significantly shorter in active TAOs than in inactive ones (p < 0.001). TAO patients showed significantly lower FA and higher MD, AD, and RD than HCs for both medial and lateral EOMs (p < 0.001), but not the AD value of lateral EOMs (p = 0.619). Active patients had significantly higher FA, MD, and AD than inactive patients for medial EOMs (p < 0.005), whereas only FA differed significantly in the lateral EOMs (p = 0.018). The MD, AD, and RD of optic nerves were significantly lower in TAO patients than HCs (p < 0.05), except for FA (p = 0.129). Multivariate analysis showed that the MD of medial EOMs and disease duration were significant predictors for disease activity. The combination of these two parameters showed optimal diagnostic efficiency for disease activity (area under the curve, 0.855; sensitivity, 68.4%; specificity, 96.9%). Conclusion: rs-EPI-based DTI is promising in assessing microstructural changes of EOMs and optic nerves and can help to indicate the disease activity of TAO, especially through the MD of medial EOMs.

Thermo-Sensitive Polyurethane Membrane with Controllable Water Vapor Permeation for Food Packaging

Hu Zhou,Huan Huan Shi,Hao Jun Fan,Jian Zhou,Ji Xin Yuan 한국고분자학회 2009 Macromolecular Research Vol.17 No.7

Suppression of Ku80 Correlates with Radiosensitivity and Telomere Shortening in the U2OS Telomerase-negative Osteosarcoma Cell Line

Hu, Liu,Wu, Qin-Qin,Wang, Wen-Bo,Jiang, Huan-Gang,Yang, Lei,Liu, Yu,Yu, Hai-Jun,Xie, Cong-Hua,Zhou, Yun-Feng,Zhou, Fu-Xiang Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.2

Ku70/80 heterodimer is a central element in the nonhomologous end joining (NHEJ) DNA repair pathway, Ku80 playing a key role in regulating the multiple functions of Ku proteins. It has been found that the Ku80 protein located at telomeres is a major contributor to radiosensitivity in some telomerase positive human cancer cells. However, in ALT human osteosarcoma cells, the precise function in radiosensitivity and telomere maintenance is still unknown. The aim of this study was to investigate the effects of Ku80 depletion in the U2OS ALT cell line cell line. Suppression of Ku80 expression was performed using a vector-based shRNA and stable Ku80 knockdown in cells was verified by Western blotting. U2OS cells treated with shRNA-Ku80 showed lower radiobiological parameters (D0, Dq and SF2) in clonogenic assays. Furthermore, shRNA-Ku80 vector transfected cells displayed shortening of the telomere length and showed less expression of TRF2 protein. These results demonstrated that down-regulation of Ku80 can sensitize ALT cells U2OS to radiation, and this radiosensitization is related to telomere length shortening.

Aerodynamic properties of a streamlined bridge-girder under the interference of trains

Huan Li,Xu-hui He,Liang Hu,Xiaojun Wei 한국풍공학회 2022 Wind and Structures, An International Journal (WAS Vol.35 No.3

Trains emerging on a streamlined bridge-girder may have salient interference effects on the aerodynamic properties of the bridge. The present paper aims at investigating these interferences by wind tunnel measurements, covering surface pressure distributions, near wake profiles, and flow visualizations. Experimental results show that the above interferences can be categorized into two primary effects, i.e., an additional angle of attack (AoA) and an enhancement in flow separation. The additional AoA effect is demonstrated by the upward-moved stagnation point of the oncoming flow, the up-shifted global symmetrical axis of flow around the bridge-girder, and the clockwise-deflected orientation of flow approaching the bridgegirder. Due to this additional AoA effect, the two critical AoAs, where flow around the bridge-girder transits from trailing-edge vortex shedding (TEVS) to impinging leading-edge vortices (ILEV) and from ILEV to leading-edge vortex shedding (LEVS) of the bridge-girder are increased by 4° with respect to the same bridge-girder without trains. On the other hand, the underlying flow physics of the enhancement in flow separation is the large-scale vortices shedding from trains instead of TEVS, ILEV, and LEVS governed the upper half bridge-girder without trains in different ranges of AoA. Because of this enhancement, the mean lift and moment force coefficients, all the three fluctuating force coefficients (drag, lift, and moment), and the aerodynamic spanwise correlation of the bridge-girder are more significant than those without trains.

Charge Transport in Single Crystals of Conjugated Polymers

Huanli Dong,Wenping Hu 한국고분자학회 2016 한국고분자학회 학술대회 연구논문 초록집 Vol.41 No.2

Different Tribological Behaviors of SiCp/AZ91 Composites Induced by Tailoring the Distribution of SiC Particles

Huan Liu,Nannan Lu,Xiaojun Wang,Xiaoshi Hu,Deqiang Chen 대한금속·재료학회 2021 METALS AND MATERIALS International Vol.27 No.3

In this study, the wear behaviors of the solution-treated and as-extruded SiCp/AZ91D metal matrix composites with differentsizes and volume fractions of SiC particles were systematically investigated under different wear test parameters. The SiCparticles exhibited a typical necklace-type distribution in the solution-treated composites and a homogeneous distributionin the as-extruded composites. Wear tests results showed that the wear rates of the as-extruded composites were lower thanthat of the solution-treated composites almost under all testing conditions and with the same SiC size and volume fraction. However, the dominant wear mechanisms for these two types of composites were different. The typical necklace-type distributionof SiCpin the solution-treated composites could effectively promote the occurrence of delamination owing to theweak bonding between SiCpand matrix in the particle aggregated regions. By contrast, as the bond between disperse SiCpand matrix was enhanced, the homogeneous distribution of SiCpin the as-extruded composites could promote the occurrenceof adhesion and prevent the occurrence of delamination. Moreover, a higher applied load and higher volume fraction of SiCpobviously promoted the progress of delamination for the solution-treated composites, while the extent of the adhesive wear forthe as-extruded composites was mainly associated with the increase of the applied load, sliding velocity and SiC particle size.

A heat shock cognate 70 gene in the endoparasitoid, Pteromalus puparum, and its expression in relation to thermal stress

( Huan Wang ),( Sheng Zhang Dong ),( Kai Li ),( Cui Hu ),( Gong Yin Ye ) 생화학분자생물학회 2008 BMB Reports Vol.41 No.5

Effect of heat treatment on microstructure and tensile strength of KD-II SiC fibers in argon and oxidation atmosphere

Huan Yin,Fenghao Yang,Guangmin Hu,Maozhong Yi 한양대학교 청정에너지연구소 2023 Journal of Ceramic Processing Research Vol.24 No.4

The polycarbosilane derived KD-II SiC fibers were heat treated in argon atmosphere at temperatures from 1000 °C to 1800°C and in oxidation atmosphere at temperatures from 1000 °C to 1500 °C for 1 hour, respectively. The effect of heat treatmenton microstructure of all fibers were characterized by X-ray diffraction, scanning electron microscopy, transmission electronmicroscopy, X-ray photoelectron spectroscopy and Atomic Force Microscopeand, and the tensile strength of fibers wereevaluated by monofilament tensile test and Weibull model. The results reveal that the fibers of heat treatment in argonatmosphere can maintain their original tensile strength (~2.5 GPa) up to the temperature at 1200 °C. After that, their tensilestrength starts to decline, especially when the temperature exceeds 1400 °C a sharp strength degradation is observed. Throughdetailed characterization, we find that the reasons for the strength degradation of fibers heat treatment in argon atmosphereinclude the appearance of surface defects, growth of β-SiC grains and decomposition of SiCxOy amorphous phase. Correspondingly, the fibers heat treated in oxidation atmosphere have lower tensile strength compared with those fibers heattreatment in argon atmosphere at the same temperature. Furthermore, their tensile strength starts to degenerate at 1000 °C,and only 30% of original strength is retained when the temperature reached 1500 °C. Obviously, for fibers heat treatment inoxidation atmosphere, their tensile strength degradation starting temperature is lower and degradation speed is faster thanthose fibers heat treatment in argon atmosphere. This can be attributed to the interface stress existed between silica layer withinternal fibers, the surface cracks caused by the thermal stress and the growth of β-SiC grains.

증강현실 기술과 북 디자인 시각화에 관한 연구

장환영(Huan Ying Zhang),왕운호(Yun Hu Wang) 한국디자인리서치학회 2024 한국디자인리서치 Vol.9 No.2

미디어 컨버전스 시대의 도래와 함께, 증강현실 기술이 다양한 분야에서 널리 활용되고 있다. 새로운 시대의 발전에 맞춰, 전통적인 디자인 형태도 혁신되어야 한다. 본 연구의 연구 목적은 AR 기술을 통합하여, 북 디자인 시각화를 구현하는 것이며, AR 기술이 어떻게 북 디자인을 시각화하고 새로운 형태의 북 디자인에 새로운 가능성을 제시하며, 소비자에게 새로운 감성적 영감을 불러일으킬 수 있는지 살펴보고자 하는 것이다. 본 연구에서는 문헌분석, 사례분석, 실증분석, 설문 조사를 통해, 북 디자인 시각화에서 AR 증강현실 기술이 직면한 과제를 찾아내고, AR 기술 및 북 디자인 시각화 연구 모델을 구축하고 가설을 설정하여, 모델의 타당성을 분석한다. 마지막으로, 설문 조사와 PSSUQ 사용성 척도를 이용하여, 결과를 평가하고 검증한다. 연구 결과에 따르면, AR 기술을 기반으로 한, 북 디자인 시각화 시스템은 현재 북 디자인이 직면하고 있는 한계를 깨고, 소비자의 단일독서 형태를 바꾸고, 더 많은 소비자의 독서 참여 및 국민 독서율을 높일 수 있다. 그리고, 디자이너의 혁신적인 디자인 컨셉에 대한 참고자료를 제공할 수 있으며, 소비자에게 풍부하고 다차원적인 상호작용 경험을 제공할 뿐만 아니라, 향후 개발의 새로운 트렌드에 적용할 수 있을 것이다. With the advent of the era of integrated media, augmented reality(AR) has been applied in many fields, and has put forward innovative requirements for traditional design forms. The research goal of this paper is to integrate AR technology to realize the visualization of book design, provide new possibilities for new forms of book design, create emotional resonance for consumers, and have certain reference value for the development of book design and the sustainable development of cultural industry. First, this paper uses literature analysis, case analysis, empirical analysis, and questionnaire survey methods to give a relevant overview of AR technology and book design visualization, analyze its research status and application cases. Secondly, using the model hypothesis method, a research model of AR technology and book design visualization was constructed, and the feasibility of the model was analyzed. Finally, the results were evaluated and verified using the survey questionnaire and PSSUQ usability scale. The following conclusions were drawn through the study. First, AR technology can break through the limitations of book design, prompt consumers to change from passive reading to active interaction, broaden the reading perspective, attract more participants, and increase the reading rate. Second, establishing a book design visualization system model with AR technology will have important significance and role, and the model will provide reference value for designers' innovative design concepts. Bring consumers a rich and multi-dimensional interactive experience and adapt to new trends in future development.