http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
Studies on the Genetic Variation in Erythrocyte Lysate by two - Dimensional gel Electrophoresis
Hong, Sung Soo,Lee, Chung Choo,Oh, Moon You,Kim, Se Jae 한국유전학회 1988 Genes & Genomics Vol.10 No.4
Two-dimensional gel electrophoresis followed by silver-staining has been employed to study 59 red cell lysates for genetic variation in Cheju population. Forty-nine polypeptides selected without respect to variability were considered suitable for scoring. Of the total of 2,891 polypeptides, 98 exhibited the combination of a normal and a variant polypeptide. All variants were present in either the father or the mother of the subjects. The observed index of heterozygosity was 3.3%±0.23%. The heterozygosity in Korean (Cheju) population was compared with those in other populations.
Sung, Yun-Hee,Chang, Hyun-Kyung,Kim, Sung-Eun,Kim, You-Mi,Seo, Jin-Hee,Shin, Min-Chul,Shin, Mal-Soon,Yi, Jae-Woo,Shin, Dong-Hoon,Kim, Hong,Kim, Chang-Ju The Korean Society of Food Science and Nutrition 2009 Journal of medicinal food Vol.12 No.4
Corni fructus is the fruit of Cornus officinalis Sieb. et Zucc, which is classified into the dogwood family of Cornaceae. Corni fructus has antineoplastic, antioxidative, and antidiabetic effects, but its anti-inflammatory and analgesic effects are unknown. Here, we investigated the anti-inflammatory and analgesic effects of an aqueous extract of corni fructus using murine RAW 264.7 macrophage cells. For this study, we used the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay, western blot analysis, prostaglandin (PG) $E_2$ immunoassay, and nitric oxide (NO) detection. In addition, the analgesic effect of corni fructus was assessed by the acetic acid-induced writhing response in mice. The aqueous extract of corni fructus suppressed $PGE_2$ synthesis and NO production by inhibiting the lipopolysaccharide-induced expression of cyclooxygenase (COX)-2 and inducible NO synthase (iNOS) in murine RAW 264.7 macrophage cells. The extract also suppressed increases in nuclear factor-${\kappa}B$ (NF-${\kappa}B$) levels in the nucleus. In vivo study showed that the extract suppressed the acetic acid-induced writhing response in mice. The aqueous extract of corni fructus exerts anti-inflammatory and analgesic effects by suppressing COX-2 and iNOS expression through the down-regulation of NF-${\kappa}B$ binding activity.
Hong, Seokchan,Lee, Hyun Woong,Chang, Dong-Yeop,You, Sooseong,Kim, Jihye,Park, Jun Yong,Ahn, Sang Hoon,Yong, Dongeun,Han, Kwang-Hyub,Yoo, Ook Joon,Shin, Eui-Cheol The American Association of Immunologists, Inc. 2013 JOURNAL OF IMMUNOLOGY Vol.191 No.1
<P>Although studies investigating the nature of Ab-secreting cells (ASCs) during acute infection with influenza or dengue virus found that the ASC response was dominated by virus-specific IgG secretion, the Ag specificity and phenotype of ASCs during primary acute viral infection were not identified. To this end, we investigated the nature of ASCs in direct ex vivo assays from patients with acute hepatitis A caused by primary infection with hepatitis A virus (HAV). We found that the frequency of CD27<SUP>high</SUP>CD38<SUP>high</SUP> ASCs was markedly increased in the peripheral blood during the acute phase of HAV infection. Moreover, substantial numbers of ASCs were non-HAV–specific and dominantly secreted IgM. We detected HAV-specific ASCs by staining with fluorochrome-tagged HAV-VP1 protein. As compared with HAV-specific ASCs, non-HAV–specific ASCs were Ki-67<SUP>low</SUP>CD138<SUP>high</SUP>CD31<SUP>high</SUP>CD38<SUP>high</SUP>, demonstrating that non-HAV–specific ASCs had a bone marrow plasma cell–like phenotype whereas HAV-specific ASCs had a phenotype typical of circulating plasmablasts. These data suggest that non-HAV–specific ASCs might be mobilized plasma cells from the bone marrow or the spleen, whereas HAV-specific ASCs were newly generated plasmablasts. In this study, we provide evidence that pre-existing plasma cells are released into the circulation and contribute to Ag-nonspecific secretion of IgM during primary HAV infection.</P>
Hong, Sung Noh,Park, Changho,Park, Soo Jung,Lee, Chang Kyun,Ye, Byong Duk,Kim, You Sun,Lee, Seungbok,Chae, Jeesoo,Kim, Jong-Il,Kim, Young-Ho BMJ Publishing Group Ltd 2016 Gut Vol.65 No.5
<P>Objective Genome wide association studies (GWAS) and meta-analyses for Crohn's disease (CD) have not fully explained the heritability of CD, suggesting that additional loci are yet to be found and that the known loci may contain high effect rare risk variants that have thus far gone undetected by GWAS. While the cost of deep sequencing remains too high to analyse many samples, targeted sequencing of pooled DNA samples allows the efficient and cost effective capture of all variations in a target region. Design We performed pooled sequencing in 500 Korean CD cases and 1000 controls to evaluate the coding exon and 50 and 30 untranslated regions of 131 CD associated genes. The identified genetic variants were validated using genotyping in an independent set of 500 CD cases and 1000 controls. Results Pooled sequencing identified 30 common/low single nucleotide variants (SNVs) in 12 genes and 3 rare SNVs in 3 genes. Our results confirmed a significant association of CD with the following previously reported risk loci: rs3810936 in TNFSF15 (OR= 1.83, p< 2.2x10(-16)), rs76418789 in IL23R (OR= 0.47, p= 1.14x10(-8)) and rs2241880 in ATG16L1 (OR= 1.30, p= 5.28x10(-6)). In addition, novel loci were identified in TNFSF8 (rs3181374, OR= 1.53, p= 1.03x10(-14)), BTNL2 (rs28362680, OR= 1.47, p= 9.67x10(-11)), HLA-DQA2 (rs3208181, OR= 1.36, p= 4.66x10(-6)), STAT3 (rs1053004, OR= 1.29, p= 2.07x10(-5)), NFKBIA (rs2273650, OR= 0.80, p= 3.93x10(-4)), NKX2-3 (rs888208, OR= 0.82, p= 6.37x10(-4)) and DNAH12 (rs4462937, OR= 1.13, p= 3.17x10(-2)). A novel rare SNV, rs200735402 in CARD9, was shown to have a protective effect (OR= 0.09, p= 5.28x10(-5)). Conclusions Our deep resequencing of 131 CD associated genes confirmed 3 reported risk loci and identified 8 novel risk loci for CD in Koreans, providing new insights into the genetic architecture of CD.</P>
( Sang Bae Lee ),( Ji Hong You ),( Minyoung Lee ),( Eun Jung Kim ),( Min Jin Kim ),( Minkyung Kim ),( Ji Sun Nam ),( Jong Suk Park ),( Chul Woo Ahn ),( Kyung Rae Kim ) 대한내과학회 2016 대한내과학회 추계학술대회 Vol.2016 No.1
Objectives:?Red blood cell distribution width (RDW) is a measure of the size variability of circulating erythrocytes, recent studies have shown that increased RDW levels are independent predictors of overall and cardiovascular disease in the general and high risk populations. CVD is one of the most common causes of mortality in patients with type 2 diabetes,and carotid atherosclerosis determined by intima media thickness (IMT) is used to predict CVD risk and related outcomes. However, no study has investigated the association between RDW and IMT in type 2 diabetic patients.Therefore, we aimed to investigate the relationship between RDW and subclinical atherosclerosis in patients with type 2 diabetes.?Methods:?We analyzed the data of 469 patients with type 2 diabetes from Seoul, Korea. We excluded subjects with a history of cardiovascular or cerebrovascular disease. Anthropometric and various biochemical profiles including RDW were measured. B-mode ultrasound measurement of carotid intima-media thickness (C-IMT) was used to evaluate subclinical atherosclerosis. Carotid atherosclerosis was defined as C-IMT ≥ 1.0 mm, as assessed by B-mode ultrasound.?Results:?The subjects were stratified into 3 groups according to RDW values. Compared with subjects in the lowest tertile of RDW, those in the highest tertile were older, more likely to be smokers, had higher SBP and BMI, longer duration of diabetes, and higher prevalence of hypertension. The C-IMT values for the first, second and third RDW tertiles were 0.74±0.12 mm, 0.77±0.13 mm, and 0.79±0.14 mm, respectively and showed the increasing trend across the RDW tertiles ( P for trend <0.01). In multiple linear regression analysis, RDW was independently associated with C-IMT. After adjusting for age and sex, the OR for carotid atherosclerosis in the highest tertile of RDW was significantly increased compared to that of the lowest tertile. These relationships remained significant after further adjustments for other risk factors (OR (95% CI), 1.35 (1.01-2.23), 2.04 (1.06-3.94), P for trend <0.05).?Conclusions:?This study showed that RDW is independently associated with subclinical atherosclerosis in type 2 diabetes.
Mechanism of Relaxation Via TASK-2 Channels in Uterine Circular Muscle of Mouse
Hong, Seung Hwa,Sung, Rohyun,Kim, Young Chul,Suzuki, Hikaru,Choi, Woong,Park, Yeon Jin,Ji, Ill Woon,Kim, Chan Hyung,Myung, Sun Chul,Lee, Moo Yeol,Kang, Tong Mook,You, Ra Young,Lee, Kwang Ju,Lim, Seung The Korean Society of Pharmacology 2013 The Korean Journal of Physiology & Pharmacology Vol.17 No.4
Plasma pH can be altered during pregnancy and at labor. Membrane excitability of smooth muscle including uterine muscle is suppressed by the activation of $K^+$ channels. Because contractility of uterine muscle is regulated by extracellular pH and humoral factors, $K^+$ conductance could be connected to factors regulating uterine contractility during pregnancy. Here, we showed that TASK-2 inhibitors such as quinidine, lidocaine, and extracellular acidosis produced contraction in uterine circular muscle of mouse. Furthermore, contractility was significantly increased in pregnant uterine circular muscle than that of non-pregnant muscle. These patterns were not changed even in the presence of tetraetylammonium (TEA) and 4-aminopyridine (4-AP). Finally, TASK-2 inhibitors induced strong myometrial contraction even in the presence of L-methionine, a known inhibitor of stretch-activated channels in myometrium. When compared to non-pregnant myometrium, pregnant myometrium showed increased immunohistochemical expression of TASK-2. Therefore, TASK-2, seems to play a key role during regulation of myometrial contractility in the pregnancy and provides new insight into preventing preterm delivery.
Roles of Rac and p38 kinase in the activation of cytosolic phospholipase A2 in response to PMA.
You, Hye Jin,Woo, Chang-Hoon,Choi, Eun-Young,Cho, Sung-Hoon,Yoo, Yung Joon,Kim, Jae-Hong Biochemical Society 2005 Biochemical journal Vol.388 No.2
<P>The roles of Rac and p38 kinase in the activation of cPLA2 (cytosolic PLA2) in Rat-2 fibroblasts were investigated. In the present study, we found that PMA activates cPLA2 by a Rac-p38 kinase-dependent pathway. Consistent with this, Rac, if activated, was shown to stimulate cPLA2 in a p38 kinase-dependent manner. In another experiment to understand the signalling mechanism by which the Rac-p38 kinase cascade mediates cPLA2 activation in response to PMA, we observed that PMA-induced cPLA2 translocation to the perinuclear region is completely inhibited by the expression of Rac1N17 or treatment with SB203580 (inhibitor of p38 kinase), suggesting that Rac-p38 kinase cascade acts in this instance by mediating the translocation of cPLA2. The mediatory role of p38 kinase in cPLA2 activation was further demonstrated after a treatment with anisomycin, a very effective activator of p38 kinase. Consistent with the mediatory role of p38 kinase in stimulating cPLA2, anisomycin induced the translocation and activation of cPLA2 in a p38 kinase-dependent manner.</P>