Proteomic Analysis of Global Changes in Protein Expression During Exposure of Gamma Radiation in Bacillus sp. HKG 112 Isolated from Saline Soil

( Gupta Anil Kumar ),( Rajiv Pathak ),( Bharat Singh ),( Hemlata Gautam ),( Ram Kumar ),( Raj Kumar ),( Rajesh Arora ),( Hemant K. Gautam ) 한국미생물 · 생명공학회 2011 Journal of microbiology and biotechnology Vol.21 No.6

A Gram-positive bacterium was isolated from the saline soils of Jangpura (U.P.), India, and showed high-level of radiation-resistant property and survived upto 12.5 kGy dose of gamma radiation. The 16S rDNA sequence of this strain was examined, identified as Bacillus sp. strain HKG 112, and was submitted to the NCBI GenBank (Accession No. GQ925432). The mechanism of radiation resistance and gene level expression were examined by proteomic analysis of whole-cell extract. Two proteins, 38 kDa and 86.5 kDa excised from SDS-PAGE, which showed more significant changes after radiation exposure, were identified by MALDI-TOF as being flagellin and S-layer protein, respectively. Twenty selected 2-DE protein spots from the crude extracts of Bacillus sp. HKG 112, excised from 2- DE, were identified by liquid chromatography mass spectrometry (LC-MS) out of which 16 spots showed significant changes after radiation exposure and might be responsible for the radiation resistance property. Our results suggest that the different responses of some genes under radiation for the expression of radiation-dependent proteins could contribute to a physiological advantage and would be a significant initial step towards a fullsystem understanding of the radiation stress protection mechanisms of bacteria in different environments.

Miniaturized Ultra Wide Band Microstrip Antenna with Defect Ground Structure for Brain Tumor Detection

Hemant Kumar Gupta,Vikas Maheshwari,Vandana Vikas Thakery 보안공학연구지원센터 2016 International Journal of Hybrid Information Techno Vol.9 No.11

Brain tumour is the largest problem in the world. Few researchers have worked in that area. In the paper brain tumour is detected by the three different parameter of the proposed antenna namely return-loss of the antenna, current density of the brain phantom model with tumour and without tumour and specific absorption rate (SAR) with and without tumour. None of the previous work detects a tumour by three different parameter simultaneously. Initially the proposed antenna is designed at 2.45GHz and then applies different technology to make it ultra wide band. Antenna frequency is shifted and it become the ultra wide band and gives a bandwidth of 2.89GHz.S11 parameter of antenna achieved maximum at 7.624GHz that is 45.76dB. Found current density of the antenna is 770.6A/m2, SAR of the antenna is 2.67*106Watt/m3.Brain is assumed as a single layer Brain Phantom model in which brain is considered as centre radius of sphere of 10mm radius and placed in contact with upper part of the antenna, dielectric constant of brain is assumed as 45.8.it gives a return loss with brain model is 31.89dB at 7.61GHz and current density is 801.24A/m2, SAR of the brain phantom model with antenna is 3.15*106 Watt/m3.Now triangular shape tumor is assumed with dielectric constant of the tumor is 52.06 and it gives a return loss with tumor 32.26 at 7.544GHz.current density with tumor is 960.3A/m2, SAR of the phantom model with tumor and antenna is 3.069*106A/m2.The paper detect tumors by three different parameter simultaneously that is very important aspect.

Comparative analysis of VMT genes/proteins in selected plant species with emphasis on bread wheat (Triticum aestivum L.)

Sharma Hemant,Shayaba,Kumar Rahul,Kumar Jitendra,Bhadana Deepa,Batra Ritu,Singh Rakhi,Kumar Sachin,Roy Joy K,Balyan Harindra S.,Gupta Pushpendra K. 한국유전학회 2023 Genes & Genomics Vol.45 No.11

Background In recent years, the study of molecular basis of uptake, transport and utilization of grain Fe/Zn (GFe/GZn) in wheat has been an active area of research. As a result, it has been shown that a number of transporters are involved in uptake and transport of Fe. In a recent study, knockout of a transporter gene OsVMT (VACUOLAR MUGINEIC ACID TRANSPORTER) in rice was shown to be involved in Fe homoeostasis. Objective In this study, we analysed VMT genes among six monocots and three dicots with major emphasis on wheat VMT genes (TaVMTs), taking OsVMT gene as a reference. Methods and results Using OsVMT gene as a reference, VMT genes were identified and sequence similarities were examined among six monocots and three dicots. Each VMT protein carried one functional domain and 7 to 10 distinct motifs (including 9 novel motifs). The qRT-PCR analysis showed differential expression by all the six TaVMT genes in pairs of contrasting wheat genotypes with high (FAR4 and WB02) and low (K8027 and HD3226) GFe/GZn at two different grain filling stages (14 DAA and 28 DAA). TaVMT1 genes showed up-regulation in high GFe/Zn genotypes relative to low GFe/Zn genotypes, whereas the TaVMT2 genes showed down-regulation or nonsignificant up-regulation in a few cases. Conclusions At 14 DAA, each of the six TaVMT genes exhibited higher expression in wheat genotypes with high GFe and GZn relative to those with low GFe and GZn, suggesting major role of VMT genes in improvement of grain Fe/Zn homoeostasis, thus making TaVMT genes useful for improvement in Fe/Zn in wheat grains.

COUPLED FIXED POINTS FOR MIXED $g$-MONOTONE UNDER RATIONAL CONTRACTIVE EXPRESSIONS IN PARTIALLY ORDERED METRIC SPACES

Hemant Kumar Nashine,Anita Gupta 영남수학회 2016 East Asian mathematical journal Vol.32 No.5

We propose coupled fixed point theorems for maps satisfying contractive conditions involving a rational expression in the setting of partially ordered metric spaces. We also present a result on the existence and uniqueness of coupled fixed points. In particular, it is shown that the results existing in the literature are extend, generalized, unify and improved by using mixed monotone property. Given to support the useability of our results, and to distinguish them from the known ones.

COUPLED FIXED POINTS FOR MIXED g-MONOTONE UNDER RATIONAL CONTRACTIVE EXPRESSIONS IN PARTIALLY ORDERED METRIC SPACES

Nashine, Hemant Kumar,Gupta, Anita The Youngnam Mathematical Society 2016 East Asian mathematical journal Vol.32 No.5

We propose coupled fixed point theorems for maps satisfying contractive conditions involving a rational expression in the setting of partially ordered metric spaces. We also present a result on the existence and uniqueness of coupled fixed points. In particular, it is shown that the results existing in the literature are extend, generalized, unify and improved by using mixed monotone property. Given to support the useability of our results, and to distinguish them from the known ones.

Methanolic Extract of Plumbago Zeylanica - A Remarkable Antibacterial Agent Against Many Human and Agricultural Pathogens

Mukesh Kumar Singh,Ajit Pandey,Hemant Sawarkar,Anshita Gupta,Bina Gidwani,Hemant Dhongade,Dulal Krishna Tripathi 대한약침학회 2017 Journal of pharmacopuncture Vol.20 No.1

Objectives: The current investigation was carried out to determine the cytotoxic and the antimicrobial activities of methanolic extracts of Plumbago zeylanica. Methods: The stems, leaves, and whole plants were air dried and extracted with methanol by using a Soxhlet extractor for 72 hours at 55 - 60°C. The antimicrobial activities were determined from the zones of inhibition, which were measured by using the agar well diffusion method, and the cytotoxicity assays were performed using the 3-(4,5-dimethylthiazol- 2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay method. Results: The methanolic extracts of the stem and the leaves of Plumbago zeylanica were tested against six bacterial species and nine fungal species, and both extracts showed antimicrobial activity in a dose-dependent manner. The leaf extract of Plumbago zeylanica showed maximum antimicrobial activity against both Staphylococcus aureus sub sp aureus and Fusarium oxysporum. The stem extract was found to be more antimicrobial against the Pseudomonas aeruginosa and the Penicillium expansum species. MTT assays were used to test the cytotoxicity of the whole plant extract in the HCT-116 and the K-562 cell lines, and that extract was shown to have weak cytotoxicity in both cell lines. Conclusion: In the present study, the methanolic stem extracts of Plumbago zeylanica were found to possess remarkable antibacterial activities against many human and agricultural pathogens. The extracts were also found to possess significant antifungal activities, but the antifungal activities were less than the antibacterial activities. Finally, the extracts were found to have weak cytotoxicities in the HCT-116 and the K-562 cell lines.

Methanolic Extract of Plumbago Zeylanica - A Remarkable Antibacterial Agent Against Many Human and Agricultural Pathogens

Singh, Mukesh Kumar,Pandey, Ajit,Sawarkar, Hemant,Gupta, Anshita,Gidwani, Bina,Dhongade, Hemant,Tripathi, Dulal Krishna KOREAN PHARMACOPUNCTURE INSTITUTE 2017 Journal of pharmacopuncture Vol.20 No.1

Objectives: The current investigation was carried out to determine the cytotoxic and the antimicrobial activities of methanolic extracts of Plumbago zeylanica. Methods: The stems, leaves, and whole plants were air dried and extracted with methanol by using a Soxhlet extractor for 72 hours at $55-60^{\circ}C$. The antimicrobial activities were determined from the zones of inhibition, which were measured by using the agar well diffusion method, and the cytotoxicity assays were performed using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay method. Results: The methanolic extracts of the stem and the leaves of Plumbago zeylanica were tested against six bacterial species and nine fungal species, and both extracts showed antimicrobial activity in a dose-dependent manner. The leaf extract of Plumbago zeylanica showed maximum antimicrobial activity against both Staphylococcus aureus sub sp aureus and Fusarium oxysporum. The stem extract was found to be more antimicrobial against the Pseudomonas aeruginosa and the Penicillium expansum species. MTT assays were used to test the cytotoxicity of the whole plant extract in the HCT-116 and the K-562 cell lines, and that extract was shown to have weak cytotoxicity in both cell lines. Conclusion: In the present study, the methanolic stem extracts of Plumbago zeylanica were found to possess remarkable antibacterial activities against many human and agricultural pathogens. The extracts were also found to possess significant antifungal activities, but the antifungal activities were less than the antibacterial activities. Finally, the extracts were found to have weak cytotoxicities in the HCT-116 and the K-562 cell lines.

Designing of Low Pass FIR filter for Processing of EMG Signal using Standard DSP Processor and Code Composer Studio

Manvendra Singh,Hemant Kumar Gupta 한국멀티미디어학회 2010 한국멀티미디어학회 국제학술대회 Vol.2010 No.-

Digital signal processing (DSP) is the study of signals in a digital representation and the processing methods of these signals. A digital filter uses a digital processor to perform numerical calculations on sampled values of the signal. To achieve the desired filtering effect, Digital filters can achieve virtually any filtering effect that can be expressed as a mathematical function or algorithm. In this paper, we have filtered the EMG signals using FIR low pass filter and then amplified the filtered samples of EMG, using multiplication process through a program written in C language. For the purpose of designing the required FIR filter, first the coefficients of the filter are determined. MATLAB program has been used to determine the coefficients of the desired FIR filter by using the filter designing tool box of MATLAB. These coefficients reveal the characteristics of the desired filter. Although the frequency response characteristics possess by ideal filter may be desirable, they are not absolutely necessary in most practical applications if we relax these conditions, it is possible to realize causal filters that approximate ideal filters as closely as we desired. In this paper, we developed a digital FIR low pass filter having the cutoff frequency 120㎐ applicable on very small voltage signal like EMG. The faithful amplification is also been achieved through the digital amplification process. From our result studies, we observe that the proposed method is highly effective & efficient for EMG signal.