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Enhanced Supercapacitive Performance of Carbonized Polyaniline by Conducting Wrapping
Zhihong Luo,Xiangqun Zhuge,Yuzhen Zhao,Meixiao He,Lihua Zhu,Heiqing Tang,Kun Luo 성균관대학교(자연과학캠퍼스) 성균나노과학기술원 2015 NANO Vol.10 No.8
Functionalized carbon nanotubes (MWNT–COOH) was selected as conducting wrapping agent to prepare composites of MWNT–COOH and carbonized polyaniline (C–PANI), to improve the supercapacitive performance of C–PANI. The morphology was characterized by scanning electron microscope and transmission electron microscope which showed that C–PANI was trapped in the conductive network of MWNT–COOH. The electrochemical measurement results indicated the increased specific capacitance and enhanced cycling stability of C–PANI with the addition of MWNT–COOH. When the weight ratio of C–PANI and MWNT–COOH was 4:1, the specific capacitance of the composites was 149 F g-1, and the capacitance retention was 93.7% after 1000 charge–discharge cycling.
Fu, Dejun,Wu, Dawei,Zhang, Zhihong,Meng, Xianquan,He, Mengbing,Guo, Huaixi,Peng, Yougui,Fan, Xiangjun The Korean Vacuum Society 1998 Applied Science and Convergence Technology Vol.7 No.1
We prepared $C_3N_4$ films by rf plasma enhanced chemical vapor deposition(PCVD) and alternating $C_3N_4$/TiN composite films by dc magnetron sputtering. X-ray diffraction (XRD) and transmission electron diffraction (TED) revealed that the structure of the films is amorphous or polycrystalline, depending on deposition conditions and heat treatment. X-ray photoelectron spectroscopy (XPS) and Fourier transform infrared (FTIR) spectroscopy confirmed the presence of $sp_3\; and sp _2$ hybridized C atoms bonded with N atoms in the tetrahedral and hexagonal configurations, respectively. Graphite-free $C_3N_4$ films were obtained by PCVD under optimal conditions. To prepare well crystallized $C_3N_4$ films by magnetron sputtering, we introduced negatively biased gratings in the sputtering system. CN films deposited at grating voltages (Vg) lower than 400V are amorphous. Crystallites of cubic and $\beta$-$C_3N_4$ were formed at increased voltages.
( Yunlong Sun ),( Yali Niu ),( Bin He ),( Long Ma ),( Ganghua Li ),( Van-tuan Tran ),( Bin Zeng ),( Zhihong Hu ) 한국미생물 · 생명공학회 2019 Journal of microbiology and biotechnology Vol.29 No.2
Currently, the genetic modification of Aspergillus oryzae is mainly dependent on protoplast-mediated transformation (PMT). In this study, we established a dual selection marker system in an industrial A. oryzae 3.042 strain by using Agrobacterium tumefaciens-mediated transformation (ATMT). We first constructed a uridine/uracil auxotrophic A. oryzae 3.042 strain and a pyrithiamine (PT)-resistance binary vector. Then, we established the ATMT system by using uridine/uracil auxotrophy and PT-resistance genes as selection markers. Finally, a dual selection marker ATMT system was developed. This study demonstrates a useful dual selection marker transformation system for genetic manipulations of A. oryzae 3.042.
Boundary layer effect of light transmission through ionic liquid under electric field
Zhou Ji,Zheng Yi,Dong Shikui,He Zhihong,Liu Bao,Zhang Yanhu 한국물리학회 2023 Current Applied Physics Vol.52 No.-
The field strength distribution in the ionic liquid boundary layer under the action of an external electric field is non-uniform. Whether its transmittance (absorption coefficient) spatially varies arouses our interest in exploring the underlying laws. In this paper, with the help of an infrared microscope, a special experimental device was designed and established. The transmittance in the ionic liquid (methyl propyl imidazole iodide, C3MImI) at different distances from the electrodes (cathode, anode) was measured under electric voltages from 0 V to 0.5 V. Results showed that transmittances of ionic liquid at the boundary layer present nonlinear change with the nonlinear electronic strength under the different applied voltage, and there is a critical threshold (0.2 V) distinguishes the sensitive and non-sensitive responses to the electric field. Our study provides a physical basis for microscale electro-optic regulation and is significant, especially for precise regulation of light field transmittance in micro-nano channels/structures.
Jiankun Yu,Lin Zhao,Yanlin Li,Na Li,Miao He,Xuefeng Bai,Zhaojin Yu,Zhihong Zheng,Xiaoyi Mi,En-Hua Wang,Minjie Wei 한국유방암학회 2013 Journal of breast cancer Vol.16 No.3
Purpose: Fanconi anemia complementation group F (FANCF) is a key factor to maintaining the function of Fanconi anaemia/BRCA (FA/BRCA) pathway, a DNA-damage response pathway. However,the functional role of FANCF in breast cancer has not been elucidated. In the present study, we evaluated the chemosensitization effect of FANCF in breast cancer cells. Methods: We performed specific knockdown of the endogenous FANCF in breast cancer cells by transfecting the cells with an FANCF short hairpin RNA (shRNA) vector. Cell viability was measured with a Cell Counting Kit-8, and DNA damage was assessed with the alkaline comet assay. The apoptosis, cell cycle, and drug accumulation were measured by flow cytometric analysis. Protein expression levels were determined by Western blot analysis, using specific antibodies. Results: The analyses of two breast cancer cell lines (MCF-7 and MDA-MB-435S) demonstrated that the FANCF shRNA could effectively block the FA/BRCA pathway through the inhibition of Fanconi anemia complementation group D2ubiquitination. Moreover, FANCF silencing potentiated the sensitivity of cells to mitomycin C (MMC), where combined FANCF shRNA/MMC treatment inhibited cell proliferation, induced Sphase arrest, apoptosis, and DNA fragmentation, and reduced the mitochondrial membrane potential, compared with MMC treatment alone. Conclusion: Taken together, this study demonstrates that the inhibition of FANCF by its shRNA leads to a synergistic enhancement of MMC cytotoxicity in breast cancer cells. These results suggest that the inhibition of the FA/BRCA pathway is a useful adjunct to cytotoxic chemotherapy for the treatment of breast cancer.
Yubao Zhang,Zhongkui Xie,John D Fletcher,Yajun Wang,Ruoyu Wang,Zhihong Guo,Yuhui He 한국식물병리학회 2020 Plant Pathology Journal Vol.36 No.1
Cucumber mosaic virus (CMV) is damaging to the growth and quality of lettuce crops in Lanzhou, China. Recently, however, for the first time an isolate of lettuce necrotic yellows virus (LNYV) has been detected in lettuce crops in China, and there is concern that this virus may also pose a threat to lettuce production in China. Consequently, there is a need to develop a rapid and efficient detection method to accurately identify LNYV and CMV infections and help limit their spread. Reverse transcription loop-mediated isothermal amplification (RT-LAMP) assays were developed to detect the nucleoprotein (N) and coat protein (CP) genes of LNYV and CMV, respectively. RT-LAMP amplification products were visually assessed in reaction tubes separately using green fluorescence and gel electrophoresis. The assays successfully detected both viruses in infected plants without cross reactivity recorded from either CMV or LNYV or four other related plant viruses. Optimum LAMP reactions were conducted in betaine-free media with 6 mM Mg2+ at 65°C for LNYV and 60°C for 60 min for CMV, respectively. The detection limit was 3.5 pg/ml and 20 fg/ml using RT-LAMP for LNYV and CMV plasmids, respectively. Detection sensitivity for both RT-LAMP assays was greater by a factor of 100 compared to the conventional reverse transcription polymerase chain reaction assays. This rapid, specific, and sensitive technique should be more widely applied due to its low cost and minimal equipment requirements.
pinghe,Yue Ma,Hongyan Dai,Linguang Li,Yuexue Liu,He Li,Guiling Zhao,Zhihong Zhang 한국식물학회 2012 Journal of Plant Biology Vol.55 No.1
Retrotransposons are the most abundant mobile elements in the plant genome and seem to play an important role in genome reorganization induced by environmental challenges. Their success in this function depends on the ability of their promoters to regulate plant adaptation to biotic and abiotic stresses. In this study, the promoter region of FaRE1 was amplified in the strawberry genome, and promoter::GUS fusion was constructed. We produced transgenic strawberry plants carrying FaRE1 promoter::GUS-fusion genes, and monitored GUS reporter activity. Histochemical and fluorimetric GUS analysis these plants showed the characteristics of the FaRE1 promoter were activated by either hormones treatments with ABA, NAA,and 2,4-D or cold stress. In addition, we found the GUS reporter was activated in the leaves of transgenic strawberry plants using 5-azaC. These results suggest that the promoter of FaRE1 may act as different signal transduction pathways,allowing FaRE1 retrotransposon to be activated in response to multiples challenges.