Culture-independent analysis of yeast diversity in Korean traditional fermented soybean foods (doenjang and kanjang) based on 26S rRNA sequence

Haque, Md. Azizul,Seo, Weon Taek,Hwang, Chung Eun,Lee, Hee Yul,Ahn, Min Ju,Cho, Kye Man The Korean Society for Applied Biological Chemistr 2015 Applied Biological Chemistry (Appl Biol Chem) Vol.58 No.3

The yeast-26S rRNA libraries were constructed from two different fermented soybean foods, doenjang and kanjang. A total of 42 clones, containing the partial 26S rRNA sequences, 0.6 kb in length, were sequenced and subjected to an online similarity search. All doenjang yeast (DY) clones only appeared in the Saccharomycotina class. The 21 clones from the doenjang library were classified into five groups: Debaryomyces hansenii (DY I, 76.0 %), Zygosaccharomyces pseudorouxii (DY II, 9.6 %), Candida versatilis (DY III, 4.8 %), Candida etchellsii (DY IV, 4.8 %), and Debaryomyces castellii (DY V, 4.8 %). The 21 kanjang yeast (KY) clones were affiliated with the Saccharomycotina (52.4 %), Urediniomycetes (19.0 %), Ustilaginomycetes (23.8 %), and Hymenomycetes (4.8 %) classes and divided into six groups: D. hansenii (KY I, 38.0 %), Sterigmatomyces halophilus (KY II, 19.0 %), Malassezia restricta (KY III, 23.8 %), Cryptococcus magnus (KY V, 4.8 %), and Pichia triangularis (KY VI, 9.6 %). Yeast belonging to the Saccharomycotina class was predominant (76.2 %) in fermented soybean foods, doenjang and kanjang. These findings are of fundamental value for understanding the complexity of two different fermented soybean foods.

Xenotransplantation of layer-by-layer encapsulated non-human primate islets with a specified immunosuppressive drug protocol

Haque, M.R.,Kim, J.,Park, H.,Lee, H.S.,Lee, K.W.,Al-Hilal, T.A.,Jeong, J.H.,Ahn, C.H.,Lee, D.S.,Kim, S.J.,Byun, Y. Elsevier Science Publishers 2017 Journal of controlled release Vol.258 No.-

<P>Islet transplantation is as effective as but also less immunogenic than pancreas transplantation for the treatment of type 1 diabetes mellitus. However, as the complete elimination of immunogenicity still remains a major obstacle in islet transplantation, layer-by-layer encapsulation (LbL) of pancreatic islets using biocompatible polymers offers a rational approach to reducing host immune response towards transplanted islets. We investigated the effect of LbL of non-human primate (NHP) islets on reducing immunogenicity as a preclinical model since NHPs have close phylogenetic and immunological relationship with humans. LbL with three-layers of polyethylene glycol (PEG) molecules (SH-6-arm-PEG-NHS, 6-arm-PEG-catechol and linear PEG-SH) showed a uniform nano-shielding on islets without the loss of viability or function of islets. An immunosuppressive drug protocol was also combined to improve the survival rate of the transplanted islets in vivo. A xenorecipient (C57BL/6 mice) of LbL islet transplanted along with our immunosuppressive drug protocol showed 100% survival rate for 150 days after transplantation. On the other hand, naked islet recipients showed poor survival time of 5.5 +/- 1.4 days without drugs and 77.5 +/- 42 days with the drug protocol. Immunohistochemistry of the transplanted grafts and serum cytokine concentration demonstrated less immunogenicity in the LbL islet transplanted recipients compared with the naked islet ones.</P>

Global warming as affected by incorporation of variably aged biomass of hairy vetch for rice cultivation

Haque, Md Mozammel,Biswas, Jatish Chandra,Waghmode, Tatoba R.,Kim, Pil Joo CSIRO Publishing 2016 Soil research Vol.54 No.3

<P> Hairy vetch (Vicia villosa Roth) is cultivated during the cold fallow season in paddy soils of temperate countries such as South Korea and Japan, mostly as animal feed and green manure. Information on the effect of ageing of hairy vetch incorporation in relation to greenhouse gas (GHG) emissions and global warming potential (GWP) is not available. Therefore, hairy vetch biomass of ages 183, 190, 197, and 204 days was incorporated in paddy soil to estimate GWP during rice cultivation. The emission rates of methane (CH4), carbon dioxide (CO2), and nitrous oxide (N2O) gases were monitored once a week by using the closed-chamber method. The net ecosystem carbon budget was used to estimate pure CO2 emission fluxes. Biomass production of hairy vetch was 6.5 Mg ha-1 at 204 days, which was similar to other treatments. The GWP was lower with the 204-day-old vetch biomass incorporation than with other treatments. High content of cellulose and lignin in 204-day-old hairy vetch might have affected decomposition rate and subsequently reduced GHGs emissions during rice cultivation. Our results suggest that hairy vetch can be allowed to grow for 204 days before incorporation at 3 Mg ha-1 without sacrificing rice yield, while maximising biomass production and minimising GWP during rice cultivation. </P>

Current Status and Technologies for Treating Groundwater Arsenic Pollution in Bangladesh

Haque MD Tashdedul,N. J. D. G. Reyes,전민수,김이형 한국습지학회 2022 한국습지학회지 Vol.24 No.2

Arsenic (As) contamination in groundwater is one of the main problems in Bangladesh. As toxicity causes serious human health problems such as edema, skin cancer, bladder cancer, lung cancer, hyperkeratosis, premature birth, and black foot disease. As contamination in groundwater mainly originates from the geological characteristics of the area due to the influence of anthropogenic activities. Since most of the people in Bangladesh rely on tube well for drinking water, it is necessary to investigate the current status of As pollution and identify the treatment technologies that can be used to provide arsenic-free drinking water in water-scarce areas. A total of 92 papers were reviewed in this study to present a complete overview of the recent status of groundwater As contamination in Bangladesh and different low-cost remediation technologies. A method for evaluating the relative feasibility of different treatment technologies was also utilized to determine the most appropriate technologies for groundwater As treatment in Bangladesh. The districts with the highest groundwater As contamination include Brahamanbariya, Tangail, Barisal, Pabna, Patuakhali, Kurigram, Magura, and Faridpur, with concentrations exceeding 0.05 mg/L. Only six districts had relatively low groundwater arsenic concentrations (0.01 mg/L), including Kushtia, Khagrachari, Jessore, Dinajpur, Meherpur, and Munshiganj. There were a number of technologies used for treating As in water, but aerated electrocoagulation, Mg-Fe-based hydrotalcite-like compound, and electro-chemical As remediation (ECAR) reactor were found to be the most feasible treatment methods for As. Overall, the investment, operational, and maintenance costs, availability of materials, and expertise requirements should be considered when selecting the most appropriate treatment method for As in water.

Effect of dilute alkali pretreatment on structural features and enhanced enzymatic hydrolysis of Miscanthus sinensis at boiling temperature with low residence time

Haque, Md.A.,Barman, D.N.,Kang, T.H.,Kim, M.K.,Kim, J.,Kim, H.,Yun, H.D. Academic Press 2013 Biosystems engineering Vol.114 No.3

The aim of this work was to evaluate the effect of dilute sodium hydroxide for conversion of Miscanthus sinensis to fermentable sugars at boiling temperature with low residence time. Various concentrations of NaOH (0.75%-2.5%) were applied for treatment of Miscanthus in an autoclave at 105 <SUP>o</SUP>C for 10 min. The non-cellulosic surface granules composed of lignin, waxes, and partly of hemicelluloses were exhibited in pretreated Miscanthus. The scanning electron microscopy, atomic force microscopy, and Fourier transform infrared spectroscopy studies showed that the 2.5% NaOH-treated Miscanthus exposed cellulose fibres and surface granules were removed, probably due to comprehensive disruption of the linkages between lignin and carbohydrates. The cellulose crystalline index was increased from the water-treatment to 2.0% NaOH-treatments and remarkably lowered by the 2.5% NaOH-treatment. Chemical component analysis showed that the level of cellulose increased to 70% and lignin was reduced to 4.2% by 2.5% NaOH-treated sample. The greatest removal of lignin and hemicellulose from pre-hydrolysate liquors was 74% and 55%, respectively. The reducing sugar yield with cellulase 15 FPU, β-glucosidase 30 IU, and xylanase 6 FXU g<SUP>-1</SUP> substrate was estimated as 73% and 87% for 2.0% and 2.5% NaOH-treated samples respectively. The 2.5% NaOH-treatment is judged to be effective for disrupting Miscanthus recalcitrance in this pretreatment regime.

Targeting the microglial NLRP3 inflammasome and its role in Parkinson's disease

Haque, Md. Ezazul,Akther, Mahbuba,Jakaria, Md.,Kim, In‐,Su,Azam, Shofiul,Choi, Dong‐,Kug John WileySons, Inc. 2020 Movement disorders Vol.35 No.1

<P><B>Abstract</B></P><P>Excessive activation of microglia and subsequent release of proinflammatory cytokines play a crucial role in neuroinflammation and neurodegeneration in Parkinson's disease (PD). Components of the nucleotide‐binding oligomerization domain and leucine‐rich‐repeat‐ and pyrin‐domain‐containing 3 inflammasome complex, leucine‐rich‐repeat‐ and pyrin‐domain‐containing 3, caspase‐1, and apoptosis‐associated speck‐like protein containing a CARD, are highly expressed in activated microglia in PD patient brains. Findings suggest that neurotoxins, aggregation of α‐synuclein, mitochondrial reactive oxygen species, and disrupted mitophagy are the key regulators of microglial leucine‐rich‐repeat‐ and pyrin‐domain‐containing 3 inflammasome activation and release of interleukin‐1β and interleukin‐18 caspase‐1‐mediated pyroptotic cell death in the substantia nigra of the brain. Although this evidence suggests the leucine‐rich‐repeat‐ and pyrin‐domain‐containing 3 inflammasome may be a potential drug target for treatment of PD, the exact mechanism of how the microglia sense these stimuli and initiate leucine‐rich‐repeat‐ and pyrin‐domain‐containing 3 inflammasome signaling is unknown. Here, the molecular mechanism and regulation of microglial leucine‐rich‐repeat‐ and pyrin‐domain‐containing 3 inflammasome activation and its role in the pathogenesis of PD are discussed. Moreover, the potential of both endogenous and synthetic leucine‐rich‐repeat‐ and pyrin‐domain‐containing 3 inflammasome modulators, long noncoding RNA, microRNA to develop novel therapeutics to treat PD is presented. Overall, we recommend that the microglial leucine‐rich‐repeat‐ and pyrin‐domain‐containing 3 inflammasome can be a potential target for PD treatment. © 2019 International Parkinson and Movement Disorder Society</P>

Efficiency of Meister-10 and Urea-nimin on Rice Production

Haque, S.A.,Sultana, Z. 한국토양비료학회 2009 한국토양비료학회 학술발표회 초록집 Vol.2009 No.10

Eu³⁺-activated potential red-emitting phosphor for solid-state lighting

Haque, Md.M.,Kudrat-E-Zahan, Md.,Jahan, N.A.,Lee, H.I. Wissenschaftliche Verlagsgesellschaft m. b. H 2017 OPTIK -STUTTGART- Vol.133 No.-

<P>The intense red-emitting NaCaY0.2Eu0.8(MoO4)(3) phosphor was prepared by using a sol-gel method for the first time. Thermogravimetric-diferential thermal analysis, X-ray diffraction, field-emission scanning electron microscopy, FT-IR and luminescent measurements were used to characterize the NaCaY0.2Eu0.8(MoO4)(3) particles. The resulting products obtained by using the sol-gel method have a narrower size distribution and a more regular particle shape than those obtained by using the solid- state reaction. The spectra analysis indicated that the obtained phosphor could be excited by UV light of 395 nm and visible light of 466 nm, also emits intense red light with a maximum at about 614 nm. The luminescent intensity of sol-gel derived is comparable with the solid state product and it is about 84% of solid-state product under 395 nm light excitation. Comparative study with YAG:Ce and CaS:Eu2+ phosphors, suggested that our synthesized phosphor can be used as an efficient red-emitting phosphor to compensate the red deficiency of YAG:Ce phosphor and also can serve as an alternative phosphor to replace sulfide based phosphors in the solid-state lighting applications. (C) 2016 Elsevier GmbH. All rights reserved.</P>

Aggregation-induced emission enhancement of fluorene-substituted 2,5-silole polymer and application as chemosensor

Haque Md Hasanul,Sohn Honglae 한국물리학회 2022 THE JOURNAL OF THE KOREAN PHYSICAL SOCIETY Vol.80 No.11

Herein, we report the optical properties of a novel conjugated silole polymer, containing 9-dicyanomethylenefuorene. The conjugated silole polymer exhibits aggregation-induced emission enhancement properties, as confrmed by adding diferent fractions of water to the polymer solution in tetrahydrofuran. The absolute quantum yield and average particle size of the nanoaggregates were measured and tuned by controlling the water fraction. Dynamic light scattering was used to assess the particle size of the polymer. The new polymer possesses a fexible conformation with π–π conjugation between the silole and 9-dicyanomethylenefuorene rings, as confrmed by density functional theory using the B3LYP/6-31G(d) basis set. The detection of explosives was achieved through photoluminescence quenching of the polymer nanoaggregates by adding picric acid, where a linear Stern–Volmer relationship was observed for the detection of picric acid.

Oil Prices and Terms of Trade of Saudi Arabia: An Empirical Analysis

HAQUE, Mohammad Imdadul,IMRAN, Mohammad Korea Distribution Science Association 2020 The Journal of Asian Finance, Economics and Busine Vol.7 No.9

Terms of trade is an important indicator of the welfare gains from international trade to the exporting country. Terms of trade of oil-exporting countries are hypothesized to depend primarily on oil prices. The study assesses the relation between oil prices and the terms of trade of Saudi Arabia. The study uses the Autoregressive Distributed Lag method to determine the cointegration between the country's terms of trade and oil prices for the period 2000-2018. The data for net barter terms of trade is taken from World Development Indicators and oil price is taken from Saudi Arabian Monetary Agency. The results show that oil prices and terms of trade are cointegrated and any disequilibrium between the two variables is corrected by 35% in a year. The study also reports a positive relationship between the two items, both in the short run and long run. Diagnostic tests indicate the model to be fit. The results suggest that, for a primarily oil-producing country like Saudi Arabia, the terms of trade depend on oil prices. The study fills the gap in the literature on the study of terms of trade for Saudi Arabia for the last few years, where there has been a high volatility in oil prices.