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RISS 인기검색어
- 검색키워드
- 저자 : Gurumayum, Sushma (검색결과 5 건)
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Clonal Propagation of Triploid Acorus calamus Linn. Using Dual-Phase Culture System
Ningthoujam Sandhyarani,Gurumayum Jitendra Sharma,Rajkumar Kishor 한국작물학회 2011 Journal of crop science and biotechnology Vol.14 No.3
Acorus calamus is an important medicinal plant which has been used in Indian traditional medicine since time immemorial. Various bioactive molecules, viz., acorin, α- and β–asarone, asaryldehyde, caryophylene, isoasarone, methylisoeugenol, and safrol have been isolated from this plant. However, the use of this plant for medicinal purpose has been recently banned due to the high toxic property of β-asarone. The triploid Acorus calamus is reported to be low in β-asarone content and thus found to be the ideal raw material for medicinal use. The present investigation represents our finding for successful in vitro clonal propagation of the elite triploid accessions of Acorus calamus for mass propagation. In the dual-phase culture system consisting of agar-solidified Murashige and Skoog medium overlaid by liquid fraction of the same medium, maximum multiple shoot induction was favored by supplementation of α-naphthaleneacetic acid (0.5 mg L^(-1) ) and 6-benzylaminopurine (2.0 mg L^(-1) ). In vitro rooting of the microshoots was maximum in the medium supplemented with indolebutyric acid at 2.0 mg L^(-1) . The well-rooted microshoots could be successfully hardened and transplanted in the field. This result can be reproduced and is a viable protocol for successful clonal propagation of the seedless triploid Acorus calamus for conservation and sustainable development.
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Clonal Propagation of Triploid Acorus calamus Linn. Using Dual-Phase Culture System
Sandhyarani, Ningthoujam,Kishor, Rajkumar,Sharma, Gurumayum Jitendra 한국작물학회 2011 Journal of crop science and biotechnology Vol.14 No.3
Acorus calamus is an important medicinal plant which has been used in Indian traditional medicine since time immemorial. Various bioactive molecules, viz., acorin, ${\alpha}$- and ${\beta}$-asarone, asaryldehyde, caryophylene, isoasarone, methylisoeugenol, and safrol have been isolated from this plant. However, the use of this plant for medicinal purpose has been recently banned due to the high toxic property of ${\beta}$-asarone. The triploid Acorus calamus is reported to be low in ${\beta}$-asarone content and thus found to be the ideal raw material for medicinal use. The present investigation represents our finding for successful in vitro clonal propagation of the elite triploid accessions of Acorus calamus for mass propagation. In the dual-phase culture system consisting of agar-solidified Murashige and Skoog medium overlaid by liquid fraction of the same medium, maximum multiple shoot induction was favored by supplementation of ${\alpha}$-naphthaleneacetic acid (0.5 mg $L^{-1}$) and 6-benzylaminopurine (2.0 mg $L^{-1}$). In vitro rooting of the micros hoots was maximum in the medium supplemented with indolebutyric acid at 2.0 mg $L^{-1}$. The well-rooted microshoots could be successfully hardened and transplanted in the field. This result can be reproduced and is a viable protocol for successful clonal propagation of the seedless triploid Acorus calamus for conservation and sustainable development.
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Microrhizome Induction in Acorus calamus Linn. - An Important Medicinal and Aromatic Plant
Ningthoujam Sandhyarani Devi,Rajkumar Kishor,Gurumayum Jitendra Sharma 한국원예학회 2012 Horticulture, Environment, and Biotechnology Vol.53 No.5
An effective protocol for in vitro microrhizome induction was developed for Acorus calamus. The explants,rhizome axillary buds, were cultured on dual phase Murashige and Skoog (MS) medium consisting of agar solidified phase overlaid by liquid fraction of the same medium. In this study, the effects of indole butyric acid (IBA) and α–naphthalene acetic acid (NAA) containing 2-10% (w/v) sucrose were examined on microrhizome induction. Best response was observed on the medium supplemented with 2.0 mg·L-1IBA and 60% sucrose under 16/8 hours light/dark photoperiod which produced the maximum rhizome fresh weight (0.82 g) and size (length 4.8 cm; diameter 0.55 cm) in 6weeks. The microrhizomes had 7-8 buds which were developed independent of season and each segment sprouted into roots and shoots when transplanted to soil. This protocol can be adopted for various applications, viz., large scale production of propagules of elite cytotype, in vitro conservation of the microrhizome, synthesis of secondary metabolites and for studying the biosynthetic pathways of the bioactive molecules present in the rhizomes of this important medicinal and aromatic plant.
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Disturbed homocysteine metabolism is associated with cancer
Tauheed Hasan,Reetika Arora,Aniket Kumar Bansal,Reshmee Bhattacharya,Gurumayum Suraj Sharma,Laishram Rajendrakumar Singh 생화학분자생물학회 2019 Experimental and molecular medicine Vol.51 No.-
Hyperhomocysteinemia/Homocysteinuria is characterized by an increased level of toxic homocysteine in the plasma. The plasma concentration of homocysteine is 5–15 μmol/L in healthy individuals, while in hyperhomocysteinemic patients, it can be as high as 500 μmol/L. While increased homocysteine levels can cause symptoms such as osteoporosis and eye lens dislocation, high homocysteine levels are most closely associated with cardiovascular complications. Recent advances have shown that increased plasma Hcy is also a fundamental cause of neurodegenerative diseases (including Alzheimer’s disease, Parkinson’s disease, and dementia), diabetes, Down syndrome, and megaloblastic anemia, among others. In recent years, increased plasma homocysteine has also been shown to be closely related to cancer. In this review, we discuss the relation between elevated plasma Hcy levels and cancer, and we conclude that disturbed homocysteine metabolism is associated with cancer. Future clinical perspectives are also discussed.
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Growth Performance, Carcass Traits and Meat Quality in Broilers, Fed Flaxseed Meal
Mridula, D.,Kaur, Daljeet,Nagra, S.S.,Barnwal, P.,Gurumayum, Sushma,Singh, K.K. Asian Australasian Association of Animal Productio 2011 Animal Bioscience Vol.24 No.12
The effect of flaxseed meal on broiler performance, carcass characteristics, alpha-linolenic acid contents, and organoleptic characteristics of chicken meat was studied during a 42 day experiment. Broiler chicks were randomly allotted to 4 experimental groups and fed iso-energetic and iso-nitrogenous diets containing flaxseed meal at 0, 5, 10 and 15%. Flaxseed meal did not affect the weekly body weight of broiler chicks during the first two weeks but thereafter weekly body weight reduced among flaxseed meal groups. At the end of $6^{th}$ week, birds fed on 15% flaxseed meal showed a reduction of 8% in body weight compared to the control group. Control group had significantly higher weight gain with slightly higher feed consumption and better feed conversion ratio (FCR), protein efficiency ratio (PER), and energy efficiency ratio (EER) than the flaxseed meal groups. Among the treatments, birds of 5 and 10% flaxseed meal groups had significantly better FCR, PER and EER compared to those of the 15% flaxseed meal group. The carcass characteristics data indicated a significant reduction in the eviscerated weight and breast yield at 15% flaxseed meal in the diet as compared to other dietary groups. However, the alpha-linolenic acid content in both breast and thigh meat was higher with an increasing level of flaxseed meal in the diets without affecting the sensory acceptability of meat. Based on the present study, up to 10% of flaxseed meal may be used in broiler diet to enhance the alpha-linolenic acid content in the broiler meat.
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