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DNM3OS Facilitates Ovarian Cancer Progression by Regulating miR-193a-3p/MAP3K3 Axis
Lei He,Guolin He 연세대학교의과대학 2021 Yonsei medical journal Vol.62 No.6
Purpose: Long non-coding RNAs (lncRNAs) are essential regulators in the development of ovarian cancer (OC). Nonetheless, thefunction of lncRNA DNM3 opposite strand/antisense RNA (DNM3OS) in OC remains unclear. This work aimed to investigate thebiological roles and underlying mechanisms of DNM3OS in OC. Materials and Methods: Quantitative real-time polymerase chain reaction was conducted to examine DNM3OS, microRNA(miR)-193a-3p, and mitogen-activated protein kinase 3 (MAP3K3) mRNA expression in OC tissues and cell lines. Kaplan-Meiersurvival analysis was employed to analyze the relationship between DNM3OS expression and the prognosis of OC patients. Cellcounting kit-8, 5-ethynyl-2'-deoxyuridine, and transwell experiments were conducted to monitor cell proliferation, migration,and invasion, respectively. Western blot was applied to examine epithelial-mesenchymal transition associated protein (E-cadherinand N-cadherin) expression. Luciferase reporter gene and RNA immunoprecipitation experiments were performed to confirmthe relationships among DNM3OS, miR-193a-3p, and MAP3K3. Pearson’s correlation analysis was adopted to analyze thecorrelations among DNM3OS, miR-193a-3p, and MAP3K3 mRNA. Results: DNM3OS expression was remarkably increased in OC tissues and cell lines, which was associated with the unfavorableprognosis of the patients. DNM3OS overexpression enhanced OC cell proliferation, migration, and invasion; suppressed E-cadherinprotein expression; and facilitated N-cadherin protein expression, while the transfection of miR-193a-3p mimics had the oppositeeffects. DNM3OS directly interacted with miR-193a-3p, and miR-193a-3p targeted MAP3K3 by directly binding to 3'UTR. DNM3OS could up-regulate the expression of MAP3K3 via repressing miR-193a-3p expression. Conclusion: DNM3OS, as an oncogenic lncRNA, increases the malignancy of OC cells via regulation of an miR-193a-3p/MAP3K3 axis.
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Liang Ma,Guolin Xiang,Yuan Huang,Meng-Ya He,Jianping Li,Pengbo Fu 한국공업화학회 2020 Journal of Industrial and Engineering Chemistry Vol.85 No.-
Afluidized bed has the advantages of treating largeflows, intensifying mass and heat transfer, andlowering costs. This study proposed a cyclonicfluidized bed packed with spherical activated carbonadsorbents for volatile organic compounds adsorption. Thefluidization and self-rotation of the ACparticles in a 25 mm cyclonicfluidized bed were studied with high-speed camera testing technology. Theeffects of the particle movement on the adsorption efficiency of toluene were also tested. The resultsshow that most of the particles at the inlet side of the cyclonicfluidized bed were moving up when theinlet airflow rate was greater than 2.0 m3/h. The particles began to move in clusters when the relativepacking height increased to a critical value of 0.57. Increasing the gasflow rate and the diameter andheight of the core column will increase the self-rotation speed of the total particles. The maximum selfrotationspeed of spherical adsorbents reached 1700 rad/s at the inletflow rate of 2.5 m3/h. In the case ofthe same axial velocity of the gas phase in the upper space of the core column, increasing the particle selfrotationspeed can slightly improve the adsorption efficiency. The maximum adsorption efficiencyreached 99% when the inletflow rate is 1.0 m3/h with relative packing height 0.65.
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Chinese Society of Allergy Guidelines for Diagnosis and Treatment of Allergic Rhinitis
Lei Cheng,Jianjun Chen,Qingling Fu,Shaoheng He,Huabin Li,Zheng Liu,Guolin Tan,Zezhang Tao,Dehui Wang,Weiping Wen,Rui Xu,Yu Xu,Qintai Yang,Chonghua Zhang,Gehua Zhang,Ruxin Zhang,Yuan Zhang,Bing Zhou,Do 대한천식알레르기학회 2018 Allergy, Asthma & Immunology Research Vol.10 No.4
Allergic rhinitis (AR) is a global health problem that causes major illnesses and disabilities worldwide. Epidemiologic studies have demonstrated that the prevalence of AR has increased progressively over the last few decades in more developed countries and currently affects up to 40% of the population worldwide. Likewise, a rising trend of AR has also been observed over the last 2-3 decades in developing countries including China, with the prevalence of AR varying widely in these countries. A survey of self-reported AR over a 6-year period in the general Chinese adult population reported that the standardized prevalence of adult AR increased from 11.1% in 2005 to 17.6% in 2011. An increasing number of original articles and imporclinical trials on the epidemiology, pathophysiologic mechanisms, diagnosis, management and comorbidities of AR in Chinese subjects have been published in international peer-reviewed journals over the past 2 decades, and substantially added to our understanding of this disease as a global problem. Although guidelines for the diagnosis and treatment of AR in Chinese subjects have also been published, they have not been translated into English and therefore not generally accessible for reference to non-Chinese speaking international medical communities. Moreover, methods for the diagnosis and treatment of AR in China have not been standardized entirely and some patients are still treated according to regional preferences. Thus, the present guidelines have been developed by the Chinese Society of Allergy to be accessible to both national and international medical communities involved in the management of AR patients. These guidelines have been prepared in line with existing international guidelines to provide evidence-based recommendations for the diagnosis and management of AR in China.
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Ye, Maoqing,Hu, Zheng,Fan, Ying,He, Ling,Xia, Fubao,Zou, Guolin Korean Society for Biochemistry and Molecular Biol 2004 Journal of biochemistry and molecular biology Vol.37 No.4
A new acid deoxyribonuclease (DNase) was purified from the cultured mycelia of Cordyceps sinensis, and designated CSDNase. CSDNase was purified by $(NH_4)_2SO_4$ precipitation, Sephacryl S-100 HR gel filtration, weak anion-exchange HPLC, and gel filtration HPLC. The protein was single-chained, with an apparent molecular mass of ca. 34 kDa, as revealed by SDS-PAGE, and an isoelectric point of 7.05, as estimated by isoelectric focusing. CSDNase acted on both double-stranded (ds) and single- stranded (ss) DNA, but preferentially on dsDNA. The optimum pH of CSDNase was pH 5.5 and its optimum temperature 55. The activity of CSDNase was not dependent on divalent cations, but its enzymic activity was inhibited by high concentration of the cation: $MgCl_2$ above 150 mM, $MnCl_2$ above 200 mM, $ZnCl_2$ above 150 mM, $CaCl_2$ above 200 mM, NaCl above 300 mM, and KCl above 300 mM. CSDNase was found to hydrolyze DNA, and to generate 3-phosphate and 5-OH termini. These results indicate that the nucleolytic properties of CSDNase are essentially the same as those of other well-characterized acid DNases, and that CSDNase is a member of the acid DNase family. To our knowledge, this is the first report of an acid DNase in a fungus.
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