Inhibitory Effects of Syk Transfection on Lung Cancer Cell Invasion

Peng, Chuan-Liang,Zhang, Ying,Sun, Qi-Feng,Zhao, Yun-Peng,Hao, Ying-Tao,Zhao, Xiao-Gang,Cong, Bo Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.5

Objective: Spleen tyrosine kinase (Syk) is closely related to tumor invasion and metastasis, and has been shown to have potential inhibitory effects in tumors. In this study, we constructed a eukaryotic expression vector for Syk and analyzed its effects on invasive ability of the A549 non-small cell lung cancer cell line in vitro. Methods: A fragment of Syk was obtained by RT-PCR from human lung cancer cells and cloned into the expression vector pLNCXSyk. After restriction endonuclease digestion, PCR and DNA sequencing confirmation, the recombinant Syk expression plasmid was transfected into A549 human lung cancer cells using lipofectamine protocols. After selection, the cells stably expressed Syk. Detection of Syk expression of the cells by RT-PCR, and invasive ability were examined. Results: The eukaryotic expression plamid pLNCXSyk was constructed and expressed stably in the A549 human lung cancer cells. The RT-PCR results showed that Syk mRNA expression was upregulated significantly (P<0.05). Lower invasion through a basal membrane were apparent after transfection (P<0.05). Conclusions: A eukaryotic expression plasmid to cause Syk expression in lung cancer cells can obviously inhibit their invasive ability in vitro.

A Hybrid Computational Intelligence Approach for the VRP Problem

Gang PENG,Kehan ZENG,Xiong YANG 한국정보기술융합학회 2013 JoC Vol.4 No.2

PGQ, a novel hybrid computational intelligence approach, in which Particle Swarm Optimization (PSO), Genetic Algorithm (GA) and quantum computation are integrated, is proposed to solve the Vehicle Routing Problem (VRP). In PSO, a quantum approach called QUP is proposed to update the particles. GA operators are employed to improve population quality. The simulation results indicate that the PGQ algorithm is very effective and is better than simple PSO and GA as well as PSO and GA mixed algorithm.

Experimental and modeling study of viscoelastic behaviors of magneto-rheological shear thickening fluids

Gang Rou Peng,Weihua Li,Tongfei Tian,Jie Ding,Masami Nakano 한국유변학회 2014 Korea-Australia rheology journal Vol.26 No.2

Nowadays, both Magneto-rheological Fluid (MRF) and Shear Thickening Fluid (STF) have separatelyattracted considerable interest due to the fast reversible response to either external magnetic field or abruptshearing loading. In this paper, we fabricated a combined phase of Magneto-rheological Shear ThickeningFluid (MRSTF), where the 25 wt% STF is applied as medium phase with the addition of varied fractionsof iron particle. The investigation of the dynamic behavior of this novel material under oscillatory shear waslaunched in a parallel-plate rheometer. The relevance of the dynamic behavior to strain amplitude,frequency and external magnetic field were investigated and discussed. A four-parameter viscoelastic modelwas applied to reconstruct the mechanical behavior of the MRSTF under different working conditions, andthe parameters were identified within the Matlab optimization algorithm. The comparison between theexperimental data and the model prediction results indicated that the four-parameter model could predictviscoelastic material with desired accuracy. The MRSTF exhibits features of both components, while pronemore to MRF with the inception of external field excitations.

Experimental and modeling study of viscoelastic behaviors of magneto-rheological shear thickening fluids

Peng, Gang Rou,Li, Weihua,Tian, Tong Fei,Ding, Jie,Nakano, Masami 한국유변학회 2014 Korea-Australia rheology journal Vol.26 No.2

Nowadays, both Magneto-rheological Fluid (MRF) and Shear Thickening Fluid (STF) have separately attracted considerable interest due to the fast reversible response to either external magnetic field or abrupt shearing loading. In this paper, we fabricated a combined phase of Magneto-rheological Shear Thickening Fluid (MRSTF), where the 25 wt% STF is applied as medium phase with the addition of varied fractions of iron particle. The investigation of the dynamic behavior of this novel material under oscillatory shear was launched in a parallel-plate rheometer. The relevance of the dynamic behavior to strain amplitude, frequency and external magnetic field were investigated and discussed. A four-parameter viscoelastic model was applied to reconstruct the mechanical behavior of the MRSTF under different working conditions, and the parameters were identified within the Matlab optimization algorithm. The comparison between the experimental data and the model prediction results indicated that the four-parameter model could predict viscoelastic material with desired accuracy. The MRSTF exhibits features of both components, while prone more to MRF with the inception of external field excitations.

Transcriptome profiling of Shindongjin and Sugary mutant at grain-filling stages using RNA-Seq

Feng-Peng Li,Min-Young Yoon,Gang Li,Yong-Jin Park 한국육종학회 2013 한국육종학회 심포지엄 Vol.2013 No.07

Rice (Oryza sativa) is an excellent model monocot with a known genome sequence for studying developmental seeds. In the study, the seeds of 10th day after flowering (DAF) were conducted RNA-Seq of the variety Shindongjin and Sugary mutant using RNA-seq technique. Approximately 202 and 214 million high-quality paired-end reads (101-bp in size) were generated in Shindongjin and Sugary mutant, respectively. Comprehensive analysis on the transcript levels of genes which encode starch-synthesis enzymes is fundamental for the assessment of the function of each enzyme and the regulatory mechanism of starch biosynthesis in seeds. Quantitative real-time PCR was also used to validate the expression profiles of 28 rice genes encoding six classes of enzymes, viz., ADPglucose pyrophosphorylase (AGPase), starch synthase, starch branching enzyme, starch debranching enzyme, starch phosphorylase, and disproportionating enzyme at different developmental grain- filling stages (DAF 1-14) between Shindongjin and Sugary mutant. The results showed that the expression of most of starch synthesis genes were up-regulated except the cytosolic AGPase small subunit2b (AGPS2b), which sharply decreased at grain-filling stages in Sugary mutant. These results will expand our understanding of the complex molecular and cellular events in rice grain-filling stages and provide a fundamental understanding of future studies on developmental endosperm in rice and other cereal crops.

EKF-based Carrier Tracking in One-bit Quantized GNSS Receiver

Ao Peng,Gang Ou,Jianghong Shi 보안공학연구지원센터(IJSIP) 2014 International Journal of Signal Processing, Image Vol.7 No.6

Inhibition of RIPK1-dependent regulated acinar cell necrosis provides protection against acute pancreatitis via the RIPK1/NF- κB/AQP8 pathway

Gang Wang,Peng-yu Duan,Yuan Ma,Xi-na Li,Feng-zhi Qu,Liang Ji,Xiao-yu Guo,Wang-jun Zhang,Fan Xiao,Le Li,Ji-sheng Hu,Bei Sun 생화학분자생물학회 2019 Experimental and molecular medicine Vol.51 No.-

Currently, preliminary results have confirmed the existence of receptor-interacting protein kinase 3 (RIPK3) and mixed lineage kinase domain-like protein (MLKL)-dependent necroptosis of pancreatic acinar cells during early acute pancreatitis (AP), which might be a potential target for the effective regulation of necroinflammatory injury. However, the exact effect of receptor-interacting protein kinase 1 (RIPK1)-dependent regulated acinar cell necrosis on AP is still uncertain. In our study, we first explored the changes in the degree of local and systemic inflammation in AP rats when the activation of acinar cell RIPK1 was inhibited. The RIPK1 inhibitor Nec-1 was used to treat rats, and the levels of related inflammatory markers, necrosis indicators and apoptotic indicators were measured. Changes in pancreatic nuclear factor κB (NF-κB) and aquaporin 8 (AQP8) expression were noted. Next, the expression of AQP8 in AR42J cells was inhibited, and the degree of cell necrosis and inflammatory damage was found to be significantly reduced. Most importantly, we demonstrated that the RIPK1/NF-ĸB/AQP8 axis might be a potential regulatory pathway mediating RIPK1-dependent regulated acinar cell necrosis in early AP. Finally, we used the NF-κB inhibitor PDTC and Nec-1 to treat rats in different groups and measured the degree of pathological pancreatic injury, the activation of RIPK1, and the expression of NF-κB and AQP8. In summary, we hypothesized that there might be a RIPK1/NF-ĸB/AQP8 pathway controlling RIPK1-dependent regulated necrosis of acinar cells in AP, which might be a promising therapeutic target against AP-related injury.

Genome-wide association studies approach and post-GWAS study in rice

Gang Li,Min-Young Yoon,Won-Hee Ra,Jae-Wan Park,Qiang He,Aye Aye Khaing,Xiao-Qiang Wang,Win Htet Oo,Feng-Peng Li,Byoung Kook Yun,Chang-Yong Lee,Yong-Jin Park 한국육종학회 2013 한국육종학회 심포지엄 Vol.2013 No.07

AGenome-wide association studies (GWAS) have proven a useful technique for identifying genetic loci responsible for natural variation in rice. With the fast developed next-generation sequencing technology, it is possible for people to carry out GWAS by phenotyping different traits. However, how to make full use of huge data, abandon unnecessary data, and solve the problem of data application effectively seems still an obstacle for many researchers. Taking the case of whole-genome resequencing of Korean authentic rice core set, here we present a general technological path of GWAS including: 1) a schematic view of sequencing-based GWAS in rice; 2) a user-friendly and interactive web application for GWAS in rice by the aid of experience from Arabidopsis; 3) Haplotype and association analysis of candidate genes in a certain mechanism pathway, giving 10 starch synthesis genes as example; and 4) functional validation by Trans- and Mata-Omics analysis.

Effect of Annealing on the Microstructure, Texture and Tensile Deformation Properties of Cu–3 wt%Ag–0.5 wt%Zr Thin Sheets Rolled at Room and Cryogenic Temperature

Peng Zhang,Han Wang,ShengJie Yao,ChuanJie Wang,Qiang Zhu,Gang Chen 대한금속·재료학회 2021 METALS AND MATERIALS International Vol.27 No.2

The Cu–3 wt%Ag–0.5 wt%Zr are rolled with the same reduction process at room (RT) and cryogenic (CT) temperature,respectively. The electron backscatter diffraction and transmission electron microscope are used to analyze and comparethe evolutions of material microstructure and texture. The results show that the stability of as-rolled CT sheets is differentfrom that of the RT samples, which can be verified by the lower temperature for the occurrence of recrystallization andAg-base particles precipitation. Besides the Brass, Copper and S textures which are exhibited in the two kinds of as-rolledsheets, there are also Q and P texture components in the as-rolled CT sheets. The constitutive equation basing on evolutionof dislocation mean free path (L) and dislocation cell size (dDC) with plastic strain indicates that the initial values of L, dDCand mobile dislocation density (ρm) are associated with annealing condition closely. Furthermore, the strain ranges of thestable and instable plastic deformation are related to the degree of recovery and recrystallization.

Analysis of the potential pathways and target genes in spinal cord injury using bioinformatics methods

Gang Zhao,Chunzheng Gao,Dayong Peng,Meng Chen,Guilai Zuo,Shiying Shan 한국유전학회 2016 Genes & Genomics Vol.38 No.7

Spinal cord injury (SCI) remains to be the most devastating type of trauma for patients because of long lasting disability and limited response to the acute drug administration and efforts at rehabilitation. With the purpose to identify potential targets for SCI treatment and to gain more insights into the mechanisms of SCI, the microarray data of GSE2270, including 119 raphe magnus (RM) samples and 125 sensorimotor cortex (SMTC) samples, was downloaded from the Gene Expression Omnibus database. Differentially expressed genes (DEGs) were screened inRM group and SMTC group compared with their corresponding controls, respectively. A protein–protein interaction (PPI) network was constructed based on the common DEGs identified in both RM group and SMTC group. Gene ontology (GO) and pathway enrichment analyses of the overlapping DEGs were performed. Furthermore, the common DEGs enriched in each pathway were analyzed to identify significant regulatory elements. Totally, 173 overlapping DEGs (130 up-regulated and 43 down-regulated) were identified in both RM and SMTC samples. These overlapping DEGs were enriched in different GO terms. Pathway enrichment analysis revealed that DEGs were mainly related to inflammation and immunity. CD68 molecule (CD68) was a hub protein in the PPI network. Moreover, the regulatory network showed that ras-related C3 botulinum toxin substrate 2 (RAC2), CD44 molecule (CD44), and actin related protein 2/3 complex (ARPC1B) were hub genes. RAC2, CD44, and ARPC1B may be significantly involved in the pathogenesis of SCI by participating significant pathways such as extracellular matrix-receptor signaling pathway and Toll-like receptor signaling pathway.