Computer Aided Diagnosis Based on K-means Collaborative Filtering Algorithm

Feng Xue-yuan,Li Peng,Qiao Pei-li 보안공학연구지원센터 2016 International Journal of Hybrid Information Techno Vol.9 No.4

In computer aided diagnosis (CAD) process, one of the most challenging problems is data sparsity, which leads to the diagnosis results are not reliable. This paper proposes a clustering collaborative filtering based algorithm to solve the problem of data sparsity. In this paper, we use k-means clustering algorithm to cluster the same type of patients, and then adopt collaborative filtering method to fill the missing data values for each cluster, in this way to reduce the complexity of similarity calculation of collaborative filtering. The proposed method makes full use of the information-sharing mechanism of "similar patient population" to predict and fill the missing values. A hepatitis dataset is used for evaluating the performance of the algorithm. Results indicate that the proposed algorithm has better performance for medical record data sparsity problem.

PARALLEL IMAGE RECONSTRUCTION FOR NEW VACUUM SOLAR TELESCOPE

Xue-Bao Li,Feng Wang,Yong Yuan Xiang,Yan-Fang Zheng,Ying Bo Liu,Hui Deng,Kai-fan Ji 한국천문학회 2014 Journal of The Korean Astronomical Society Vol.47 No.2

Many advanced ground-based solar telescopes improve the spatial resolution of observation images using an adaptive optics (AO) system. As any AO correction remains only partial, it is necessary to use post-processing image reconstruction techniques such as speckle masking or shift-and-add (SAA) to reconstruct a high-spatial-resolution image from atmospherically degraded solar images. In the New Vacuum Solar Telescope (NVST), the spatial resolution in solar images is improved by frame selection and SAA. In order to overcome the burden of massive speckle data processing, we investigate the possibility of using the speckle reconstruction program in a real-time application at the telescope site. The code has been written in the C programming language and optimized for parallel processing in a multi-processor environment. We analyze the scalability of the code to identify possible bottlenecks, and we conclude that the presented code is capable of being run in real-time reconstruction applications at NVST and future large aperture solar telescopes if care is taken that the multi-processor environment has low latencies between the computation nodes.

Conditional Replication of a Recombinant Adenovirus Studied Using Neomycin as a Selective Marker

( Feng Xue ),( Yi Peng Qi ),( Mallam Nock Joshua ),( Ping Lan ),( Chang Yuan Dong ) 생화학분자생물학회 2003 BMB Reports Vol.36 No.3

An E1B-defective adenovirus, named r2/Ad carrying the neo expression cassette, was constructed by homologous recombination. The construction, selection (using neomycin as a seletive marker), and propagation of the recombinant virus was performed in human embryonic kidney 293 cells (HEK 293). An in vitro study demonstrated that this recombinant virus has the ability to replicate in and lyse some p53-deficient human bladder cells (EJ), but not in some cells with functional p53, such as human adenocarcinoma cells (A549) and human fibroblast cells (MRC-5). Also, based on the cytopathic effect (CPE), it was demonstrated, under identical conditions, that the U251 cells were more sensitive to r2/Ad replication than the EJ cells. In this paper, we report that r2/Ad could be very useful in studying the in vitro selective replication of EIB-defective adenovirus and has great potential in cancer gene therapy.

Synergistic effect of fluorinated hexane as diluent of fluorinated octanol for salicylic acid extraction

Xue Wu,Zhidong Chang,Junchao Yuan,Jingge Feng,Hasan Uslu,Wenjun Li 한국공업화학회 2018 Journal of Industrial and Engineering Chemistry Vol.58 No.-

In order to get optimal extraction performance as well as ultra-low solvent residual, three fluorinated hexane, perfluorohexane (C6F14), 1H-perfluorohexane (C6F13H) and 1H,6H-perfluorohexane (C6F12H2), were evaluated as diluents for synergistic effect on extraction capability of 1H,1H,2H,2H-Perfluorooctan-1-ol by the way of experimental and computational investigation. Based on extraction experiments, C6F14 showed obvious antagonistic effect because of hydrophobic nature, while the synergistic effect of C6F12H2 was better than that of C6F13H. According to DFT calculation, supported by FT-IR and 1H NMR spectra, the synergistic effect was illustrated as that fluorinated hexane could interact with salicylic acid-fluorinated octanol complex through hydrogen bond.

Conditional Replication of a Recombinant Adenovirus Studied Using Neomycin as a Selective Marker

Xue, Feng,Qi, Yi-Peng,Joshua, Mallam Nock,Lan, Ping,Dong, Chang-Yuan Korean Society for Biochemistry and Molecular Biol 2003 Journal of biochemistry and molecular biology Vol.36 No.3

An E1B-defective adenovirus, named r2/Ad carrying the neo expression cassette, was constructed by homologous recombination. The construction, selection (using neomycin as a selective marker), and propagation of the recombinant virus was performed in human embryonic kidney 293 cells (HEK 293). An in vitro study demonstrated that this recombinant virus has the ability to replicate in and lyse some p53-deficient human tumor cells such as human glioma tumor cells (U251) and human bladder cells (EJ), but not in some cells with functional p53, such as human adenocarcinoma cells (A549) and human fibroblast cells (MRC-5). Also, based on the cytopathic effect (CPE), it was demonstrated, under identical conditions, that the U251 cells were more sensitive to r2/Ad replication than the EJ cells. In this paper, we report that r2/Ad could be very useful in studying the in vitro selective replication of E1B-defective adenovirus and has great potential in cancer gene therapy.

Rapid and Visual Detection of Vibrio parahaemolyticus in Aquatic Foods Using blaC_ARB-17 Gene-Based Loop-Mediated Isothermal Amplification with Lateral Flow Dipstick (LAMP-LFD)

( Yuan-qing Hu ),( Xian-hui Huang ),( Li-qing Guo ),( Zi-chen Shen ),( Lin-xue Lv ),( Feng-xia Li ),( Zan-hu Zhou ),( Dan-feng Zhang ) 한국미생물 · 생명공학회 2021 Journal of microbiology and biotechnology Vol.31 No.12

Vibrio parahaemolyticus is recognized as one of the most important foodborne pathogens responsible for gastroenteritis in humans. The bla_CARB-17 gene is an intrinsic β-lactamase gene and a novel species-specific genetic marker of V. parahaemolyticus. In this study, a loop-mediated isothermal amplification (LAMP) assay combined with a lateral flow dipstick (LFD) was developed targeting this bla_CARB-17 gene. The specificity of LAMP-LFD was ascertained by detecting V. parahaemolyticus ATCC 17802 and seven other non-V. parahaemolyticus strains. Finally, the practicability of LAMP-LFD was confirmed by detection with V. parahaemolyticus-contaminated samples and natural food samples. The results showed that the optimized reaction parameters of LAMP are as follows: 2.4 mmol/l Mg²⁺, 0.96 mmol/l dNTPs, 4.8 U Bst DNA polymerase, and an 8:1 ratio of inner primer to outer primer, at 63℃ for 40 min. The optimized reaction time of the LFD assay is 60 min. Cross-reactivity analysis with the seven non-V. parahaemolyticus strains showed that LAMP-LFD was exclusively specific for V. parahaemolyticus. The detection limit of LAMP-LFD for V. parahaemolyticus genomic DNA was 2.1 × 10^-4 ng/μl, corresponding to 630 fg/reaction and displaying a sensitivity that is 100-fold higher than that of conventional PCR. LAMP-LFD in a spiking study revealed a detection limit of approximately 6 CFU/ml, which was similar with conventional PCR. The developed LAMP-LFD specifically identified the 10 V. parahaemolyticus isolates from 30 seafood samples, suggesting that this LAMP-LFD may be a suitable diagnostic method for detecting V. parahaemolyticus in aquatic foods.

Enhanced (R)-2-(4-Hydroxyphenoxy)Propionic Acid Production by Beauveria bassiana: Optimization of Culture Medium and H₂O₂ Supplement under Static Cultivation

( Hai-feng Hu ),( Hai-yan Zhou ),( Xian-lin Wang ),( Yuan-shan Wang ),( Ya-ping Xue ),( Yu-guo Zheng ) 한국미생물 · 생명공학회 2020 Journal of microbiology and biotechnology Vol.30 No.8

(R)-2-(4-hydroxyphenoxy)propionic acid (HPOPA) is a key intermediate for the preparation of aryloxyphenoxypropionic acid herbicides (R-isomer). In order to improve the HPOPA production from the substrate (R)-2-phenoxypropionic acid (POPA) with Beauveria bassiana CCN-A7, static cultivation and H₂O₂ addition were attempted and found to be conducive to the task at hand. This is the first report on HPOPA production under static cultivation and reactive oxygen species (ROS) induction. On this premise, the cultivation conditions and fermentation medium compositions were optimized. As a result, the optimal carbon source, organic nitrogen source, and inorganic nitrogen source were determined to be glucose, peptone, and ammonium sulfate, respectively. The optimal inoculum size and fermentation temperature were 13.3% and 28℃, respectively. The significant factors including glucose, peptone, and H₂O₂, identified based on Plackett-Burman design, were further optimized through Central Composite Design (CCD). The optimal concentrations were as follows: glucose 38.81 g/l, peptone 7.28 g/l, and H₂O₂ 1.08 g/l/100 ml. Under the optimized conditions, HPOPA titer was improved from 9.60 g/l to 19.53 g/l, representing an increase of 2.03- fold. The results obtained in this work will provide novel strategies for improving the biosynthesis of hydroxy aromatics.

Manufacturing Enterprise Network Structure Influencing Mechanism on its Ecological Performance under Different Strategies

Wang Xue-yuan,Liu Cheng-long,Zhang Yu-feng 보안공학연구지원센터 2016 International Journal of u- and e- Service, Scienc Vol.9 No.1

Manufacturing enterprise is the core to enhance country or regional competitive advantage; meanwhile it’s the main source of environment pollution. According to configuration theory, only optimal structure can guarantee the realization of strategic target. Therefore, manufacturing enterprise network structure dimension (centrality, transparency, disparity, and complementarity) is confirmed in this paper, and its influencing mechanism on its ecological performance is discovered based on data collection from typical manufacturing enterprise network by regression analysis method. From the research result we can see that efficiency and innovation oriented manufacturing enterprise network ecological performance has different relation with network structure. Efficiency-oriented network should improve its centrality, transparency and complementarity to increase its ecological performance; while for innovation-oriented network, it should keep medium transparency and comparatively higher level disparity to realize ecological development, and the complementarity also should be improved to get higher ecological performance in long run.

Designing Ternary Laves Phase-Based Eutectic Alloys CrFeNbx with Excellent Strength, Toughness, and Thermal Stability

Yunlong Xue,Yuxuan Wang,Haohua Sun,Na Feng,Liang Yuan 대한금속·재료학회 2023 METALS AND MATERIALS International Vol.29 No.2

Three ternary Laves phase-based eutectic alloys CrFeNbx(x = 2.0, 2.35, 2.7) were designed successfully by combining thebinary phase diagrams and thermodynamics calculations. With the increasing Nb content, the solidified microstructuresevolved from the primary (Cr, Fe)2Nb plus the eutectic (Cr, Fe)2Nb/Nbss in the CrFeNb2.0,via the full eutectic (Cr, Fe)2Nb/Nbss in the CrFeNb2.35,and finally to the primary Nbss plus the eutectic (Cr, Fe)2Nb/Nbss in the CrFeNb2.7. The full eutectic(Cr, Fe)2Nb/Nbss of the CrFeNb2.35was found with outstanding thermal stability, and the lamellar eutectic was quitestable even under the heat-treatment of 1273 K/48 h. The compression strength and fracture toughness increased firstly anddecreased subsequently with the increase of Nb content, which reached their maximums of 2.54 GPa and 18.25 MPa·m1/2,respectively, in the CrFeNb2.35. The excellent combination of compression strength and fracture toughness in the CrFeNbx(x = 2.0, 2.35, 2.7) were attributed to the synergistic effects of Laves phase strengthening, interface strengthening, solidsolution strengthening, alloying toughening and Nbss phase toughening mechanisms.

Diffusion-Weighted Imaging for the Left Hepatic Lobe has Higher Diagnostic Accuracy for Malignant Focal Liver Lesions

Han, Xue,Dong, Yin,Xiu, Jian-Jun,Zhang, Jie,Huang, Zhao-Qin,Cai, Shi-Feng,Yuan, Xian-Shun,Liu, Qing-Wei Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.15

Background: This study was conducted to investigate whether apparent diffusion coefficient (ADC) measurements by dividing the liver into left and right hepatic lobes may be utilized to improve the accuracy of differential diagnosis of benign and malignant focal liver lesions. Materials and Methods: A total of 269 consecutive patients with 429 focal liver lesions were examined by 3-T magnetic resonance imaging that included diffusion-weighted imaging. For 58 patients with focal liver lesions of the same etiology in left and right hepatic lobes, ADCs of normal liver parenchyma and focal liver lesions were calculated and compared using the paired t-test. For all 269 patients, ADC cutoffs for focal liver lesions and diagnostic accuracy in the left hepatic lobe, right hepatic lobe and whole liver were evaluated by receiver operating characteristic curve analysis. Results: For the group of 58 patients, mean ADCs of normal liver parenchyma and focal liver lesions in the left hepatic lobe were significantly higher than those in the right hepatic lobe. For differentiating malignant lesions from benign lesions in all patients, the sensitivity and specificity were 92.6% and 92.0% in the left hepatic lobe, 94.4% and 94.4% in the right hepatic lobe, and 90.4% and 94.7% in the whole liver, respectively. The area under the curve of the right hepatic lobe, but not the left hepatic lobe, was higher than that of the whole liver. Conclusions: ADCs of normal liver parenchyma and focal liver lesions in the left hepatic lobe were significantly higher than those in the right hepatic lobe. Optimal ADC cutoff for focal liver lesions in the right hepatic lobe, but not in the left hepatic lobe, had higher diagnostic accuracy compared with that in the whole liver.