The Differences of Subcellular Ca²⁺ Distribution in Cucumber Seedling Leaves under NO₃⁻ Stress

Feng Juan Yang,Xiu Feng Wang,Min Wei 한국원예학회 2006 한국원예학회 기타간행물 Vol.- No.-

Banana Resistant Starch and Its Effects on Constipation Model Mice

Juan Wang,Ji Hong Huang,Yan Feng Cheng,Gong Ming Yang 한국식품영양과학회 2014 Journal of medicinal food Vol.17 No.8

Banana resistant starch (BRS) was extracted to investigate the structural properties of BRS, its effects on the gastrointestinal transit, and dejecta of normal and experimentally constipated mice. The mouse constipation model was induced by diphenoxylate administration. The BRS administered mice were divided into three groups and gavaged with 1.0, 2.0, or 4.0 g/kg body weightBRS per day. The small intestinal movement, time of the first black dejecta, dejecta granules,weight and theirmoisture content, body weight, and food intake of mice were studied. Results showed that the BRS particles were oval and spindly and some light cracks and pitswere in the surface. The degree of crystallinity of BRSwas 23.13%; the main diffraction peaks were at 2h 15.14, 17.38, 20.08, and 22.51. The degree of polymerization of BRS was 81.16 and the number-average molecular weight was 13147.92Da, as determined by the reducing terminal method. In animal experiments, BRS at the dose of 4.0 g/kg body weight per daywas able to increase the gastrointestinal propulsive rate, and BRS at the doses of 2.0 and 4.0 g/kg bodyweight per daywas found to shorten the start time of defecation by observing the first black dejecta exhaust. However, there were no influences of BRS on the dejecta moisture content, the dejecta granules and their weight, body weight, or daily food intake in mice. BRS was effective in accelerating the movement of the small intestine and in shortening the start time of defecation, but did not impact body weight and food intake. Therefore, BRS had the potential to be useful for improving intestinal motility during constipation.

A New Practical System for Evaluating the Pharmacological Properties of Uricase as a Potential Drug for Hyperuricemia

Juan Feng,Xiang Li,Xiaolan Yang,Chun Zhang,Yonghua Yuan,Jun Pu,Yunsheng Zhao,Yanling Xie,Huidong Yuan,Youquan Bu,Fei Liao 대한약학회 2010 Archives of Pharmacal Research Vol.33 No.11

The use of uricase-deficient mammals to screen formulations of engineered uricases as potential drugs for hyperuricemia involves heavy costs and presents a technical bottleneck. Herein, a new practical system was investigated to evaluate the pharmacological significance of a bacterial uricase based on its ability to eliminate uric acid in plasma in vitro, its pharmacokinetics in vivo in healthy rats, and the modeled pharmacodynamics in vivo. This uricase, before and after modification with the monomethyl ether of poly(ethylene glycol)-5000, effectively eliminated uric acid in vitro in rabbit plasma, but its action was susceptible to xanthine inhibition. After intravenous injection of the modified uricase without purification, a bi-exponential model fit well to uricase activities in vivo in the plasma of healthy rats; the half-life of the modified uricase was estimated without interference from the unmodified uricase leftover in the sample and was nearly 100-fold longer than that of the unmodified uricase. Using a model of the elimination of uric acid in vivo taking into account of uricase pharmacokinetics and xanthine inhibition, modeled pharmacodynamics supported that the half-life of uricase and its susceptibility to xanthine are crucial for the pharmacological significance of uricase. Hence,this practical system is desirable for doing preliminary screening of formulations of engineered uricases as potential drugs for hyperuricemia.

Sphingobacterium mucilaginosum sp. nov., isolated from rhizosphere soil of a rose

Yang, Jung-Eun,Won, KyungHwa,Yi, Tae-Hoo,Jin, Feng-Xie,Singh, Hina,Du, Juan Microbiology Society 2015 International journal of systematic and evolutiona Vol.65 No.9

Structure Analysis and Laxative Effects of Oligosaccharides Isolated from Bananas

Juan Wang,Hui Hua Huang,Yan Feng Cheng,Gong Ming Yang 한국식품영양과학회 2012 Journal of medicinal food Vol.15 No.10

Banana oligosaccharides (BOS) were extracted with water, and then separated and purified using column chromatography. Gel penetration chromatography was used to determine the molecular weights. Thin layer chromatogram and capillary electrophoresis were employed to analyze the monosaccharide composition. The indican bond and structure of the BOS molecule were determined using Fourier transform infrared spectroscopy and nuclear magnetic resonance. Results showed that BOS were probably composed of eight β-D-pyran glucose units linked with 1→6 indican bonds. The laxative effects of BOS were investigated in mice using the method described in “Handbook of Technical Standards for Testing and Assessment of Health Food in China.” The length of the small intestine over which a carbon suspension solution advanced in mice treated with low-, middle-, and high-dose BOS was significantly greater than that in the model group, suggesting that BOS are effective in accelerating the movement of the small intestine.

Dammarane-type triterpene oligoglycosides from the leaves and stems of Panax notoginseng and their antiinflammatory activities

Juan Li,Ru-Feng Wang,Yue Zhou,Hai-Jun Hu,Ying-Bo Yang,Li Yang,Zheng-Tao Wang 고려인삼학회 2019 Journal of Ginseng Research Vol.43 No.3

Background: Inflammation is widespread in the clinical pathology and closely associated to the progressof many diseases. Triterpenoid saponins as a key group of active ingredients in Panax notoginseng (Burk.)F.H. Chen were demonstrated to show antiinflammatory effects. However, the chemical structures ofsaponins in the leaves and stems of Panax notoginseng (PNLS) are still not fully clear. Herein, the isolation,purification and further evaluation of the antiinflammatory activity of dammarane-type triterpenoidsaponins from PNLS were conducted. Methods: Silica gel and reversed-phase C8 column chromatography were used. Furthermore, preparativeHPLC was used as a final purification technique to obtain minor saponins with high purities. MS, NMRexperiments, and chemical methods were used in the structural identifications. The antiinflammatoryactivities of the isolated saponins were assessed by measuring the nitric oxide production in RAW 264.7cells stimulated by lipopolysaccharides. Real-time reverse transcription polymerase chain reaction wasused to measure the gene expressions of inflammation-related gene. Results: Eight new minor dammarane-type triterpene oligoglycosides, namely notoginsenosides LK1-LK8 (1e8) were obtained from PNLS, along with seven known ones. Among the isolated saponins,gypenoside IX significantly suppressed the nitric oxide production and inflammatory cytokines includingtumor necrosis factor-a, interleukin 10, interferon-inducible protein 10 and interleukin-1b. Conclusion: The eight saponins may enrich and expand the chemical library of saponins in Panax genus. Moreover, it is reported for the first time that gypenoside IX showed moderate antiinflammatory activity.

Sphingomonas flavus sp. nov. isolated from road soil

Du, Juan,Singh, Hina,Won, KyungHwa,Yang, Jung-Eun,Akter, Shahina,Jin, Feng-Xie,Yi, Tae-Hoo Springer-Verlag 2015 Archives of microbiology Vol.197 No.7

<P>A yellow-colored, Gram-negative, strictly aerobic, non-motile, rod-shaped bacterium, designated THG-MM5(T), was isolated from road soil in Yongin-si, Gyeonggi-do, Republic of Korea. Based on 16S rRNA gene sequence, strain THG-MM5(T) was moderately related to Sphingomonas sediminicola KACC 15039(T) (96.1?%), Sphingomonas ginsengisoli KACC 16858(T) (96.1?%) and Sphingomonas jaspsi KACC 13230(T) (96.0?%). Chemotaxonomic data revealed that strain THG-MM5(T) possesses ubiquinone-10 as the only respiratory quinone, sym-homospermidine as the major polyamine and summed feature 3 (C16:1 ω7c and/or C16:1 ω6c), C18:1 ω7c and C16:0 as the major fatty acids. The polar lipid profile included sphingoglycolipid. The DNA G?+?C content was 60.7?mol%. These data, together with phenotypic characterization, corroborated the affiliation of strain THG-MM5(T) to the genus Sphingomonas. Thus, the isolate represents a novel species, for which the name Sphingomonas flavus sp. nov. is proposed, with THG-MM5(T) as the type strain (=KACC 18277(T)?=?CCTCC AB 2014320(T)).</P>

Hydrogenophaga luteola sp. nov. isolated from reed pond water

Du, Juan,Yang, Jung-Eun,Singh, Hina,Akter, Shahina,Won, KyungHwa,Yin, Chang Shik,Jin, Feng-Xie,Yi, Tae-Hoo Springer-Verlag 2015 Antonie van Leeuwenhoek Vol.108 No.3

Dammarane-type triterpene oligoglycosides from the leaves and stems of Panax notoginseng and their antiinflammatory activities

Li, Juan,Wang, Ru-Feng,Zhou, Yue,Hu, Hai-Jun,Yang, Ying-Bo,Yang, Li,Wang, Zheng-Tao The Korean Society of Ginseng 2019 Journal of Ginseng Research Vol.43 No.3

Background: Inflammation is widespread in the clinical pathology and closely associated to the progress of many diseases. Triterpenoid saponins as a key group of active ingredients in Panax notoginseng (Burk.) F.H. Chen were demonstrated to show antiinflammatory effects. However, the chemical structures of saponins in the leaves and stems of Panax notoginseng (PNLS) are still not fully clear. Herein, the isolation, purification and further evaluation of the antiinflammatory activity of dammarane-type triterpenoid saponins from PNLS were conducted. Methods: Silica gel and reversed-phase C8 column chromatography were used. Furthermore, preparative HPLC was used as a final purification technique to obtain minor saponins with high purities. MS, NMR experiments, and chemical methods were used in the structural identifications. The antiinflammatory activities of the isolated saponins were assessed by measuring the nitric oxide production in RAW 264.7 cells stimulated by lipopolysaccharides. Real-time reverse transcription polymerase chain reaction was used to measure the gene expressions of inflammation-related gene. Results: Eight new minor dammarane-type triterpene oligoglycosides, namely notoginsenosides LK1-LK8 (1-8) were obtained from PNLS, along with seven known ones. Among the isolated saponins, gypenoside IX significantly suppressed the nitric oxide production and inflammatory cytokines including tumor necrosis $factor-{\alpha}$, interleukin 10, interferon-inducible protein 10 and $interleukin-1{\beta}$. Conclusion: The eight saponins may enrich and expand the chemical library of saponins in Panax genus. Moreover, it is reported for the first time that gypenoside IX showed moderate antiinflammatory activity.

Proteomic Analysis of Salt Stress Responses in Rice Shoot

Xiao-Juan Li,Ming-Feng Yang,Yi Zhu,Yu Liang,Shi-Hua Shen 한국식물학회 2011 Journal of Plant Biology Vol.54 No.6

To gain a better understanding of the mechanism of rice (Oryza sativa L.) in response to salt stress, we performed a proteomics analysis of rice in response to 250 mM NaCl treatment using shoots of 3-day-old nascent seedlings. The changes of protein patterns were monitored with two-dimensional gel electrophoresis. Of 57 protein spots showing changes in abundance in response to salt stress, 52 were identified by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. The identified proteins were classified into eight functional categories. Several novel salt stress-responsive proteins, including protein synthesis inhibitor I, photosystem II stability/assembly factor HCF136, trigger factor-like protein and cycloartenol-C24-methyltransferase are upregulated upon salt stress. In order to figure out the different and similar molecular mechanism among salt and other stresses,regulation of some salt responsive proteins under other abiotic stress (cold and dehydration) and abscisic acid application was also analyzed. The possible molecular mechanism of rice seedlings in response to salinity and other stresses were discussed.