Investigation on the Biodegradability and Antibacterial Properties of Nanohybrid Suture Based on Silver Incorporated PGA-PLGA Nanofibers

Farzaneh Rouhollahi,Seyed Abdolkarim Hosseini,Farzaneh Alihosseini,Alireza Allafchian,Fatemeh Haghighat 한국섬유공학회 2018 Fibers and polymers Vol.19 No.10

Polyglycolic acid-poly lactic glycolic acid (PGA-PLGA) electrospun nanofibers containing silver nanoparticles have been produced and twisted into the nanofibrous yarn. The morphology of nanofibers and produced yarns, as well as the mechanical properties of the yarns, were investigated. Furthermore, in vitro antibacterial properties and in vitro degradation behavior of yarns containing various silver nanoparticles were studied. SEM images confirmed that the addition of the silver nanoparticles into the polymer solution increases the fiber diameters. The result of the mechanical test of the yarns alone and used in two different forms of the knots was measured and results showed that the strength of the yarns without the knot was significantly more than that of others. The biodegradability test showed that the mechanical properties and the weight of the yarns were quickly reduced after subjecting to in vitro condition. The result of the antibacterial test indicated that the nanofiber yarns containing %3 silver nanoparticles were the most appropriate sample with a considerably antibacterial activity against both gram-positive bacterium Staphylococcus aureus and gram-negative bacterium Escherichia Coli with inhibition zones of 8.1 and 9.5 mm, respectively; which demonstrated that silver nanoparticles retained their effectiveness after the electrospinning process. Therefore the nanofibrous yarns containing silver nanoparticles could be successfully produced by the electrospinning process with the proper antibacterial property as a candidate for the surgical sutures.

Inhibition of the Aspergillus flavus Growth and Aflatoxin B1 Contamination on Pistachio Nut by Fengycin and Surfactin-Producing Bacillus subtilis UTBSP1

Farzaneh, Mohsen,Shi, Zhi-Qi,Ahmadzadeh, Masoud,Hu, Liang-Bin,Ghassempour, Alireza The Korean Society of Plant Pathology 2016 Plant Pathology Journal Vol.32 No.3

In this study, the treatment of pistachio nuts by Bacillus subtilis UTBSP1, a promising isolate to degrade aflatoxin B1 (AFB1), caused to reduce the growth of Aspergillus flavus R5 and AFB1 content on pistachio nuts. Fluorescence probes revealed that the cell free supernatant fluid from UTBSP1 affects spore viability considerably. Using high-performance liquid chromatographic (HPLC) method, 10 fractions were separated and collected from methanol extract of cell free supernatant fluid. Two fractions showed inhibition zones against A. flavus. Mass spectrometric analysis of the both antifungal fractions revealed a high similarity between these anti-A. flavus compounds and cyclic-lipopeptides of surfactin, and fengycin families. Coproduction of surfactin and fengycin acted in a synergistic manner and consequently caused a strong antifungal activity against A. flavus R5. There was a positive significant correlation between the reduction of A. flavus growth and the reduction of AFB1 contamination on pistachio nut by UTBSP1. The results indicated that fengycin and surfactin-producing B. subtilis UTBSP1 can potentially reduce A. flavus growth and AFB1 content in pistachio nut.

Production of a biodiesel additive in a stirred basket reactor using immobilized lipase: Kinetic and mass transfer analysis

Farzaneh Vahabzadeh,Mohamad Hajar 한국화학공학회 2016 Korean Journal of Chemical Engineering Vol.33 No.4

A laboratory-scale stirred basket reactor (SBR) was constructed to study the synthesis of an n-butyl oleate ester using Novozym 435. An ester yield of approximately 98% was obtained after 6 h using an equimolar substrate ratio, 3.5 g of enzyme, a reaction temperature of 40 oC, and an impeller speed of 200 rpm. The kinetic data were modeled as a ping-pong bi-bi mechanism using a non-linear regression technique. Statistical analysis of the results showed that a model that incorporated the inhibitory effect of n-butanol yielded the best fit with the following parameters: Vmax=24.8mmol L−1 min−1, Km, oleic acid=190.8mM, Km, n-butanol=544.7 mM, and Ki, n-butanol=158.3mM. Mass transfer effects on the enzyme kinetics were also studied, and the absence of internal and external diffusion limitations on the reaction in the SBR was confirmed by considering calculated values of the Thiele modulus and the Damkohler number. Novozym 435 exhibited satisfactory performance in repeated-batch experiments using SBR.

Sensory and Motor Behavior Evidences Supporting the Usefulness of Conditioned Medium from Dental Pulp-Derived Stem Cells in Spinal Cord Injury in Rats

Farzaneh Dehghani,Reza Asadi-Golshan,Vahid Razban,Esmaeil Mirzaei,Abdolkarim Rahmanian,Sahar Khajeh,Zohreh Mostafavi-Pour 대한척추외과학회 2018 Asian Spine Journal Vol.12 No.5

Study Design: Experimental animal study. Purpose: This study aimed to assess effects of conditioned medium (CM) of dental pulp-derived stem cells loaded in collagen hydrogel on functional recovery following spinal cord injury (SCI). Overview of Literature: SCI affects sensory and motor functions, and behavioral recovery is the most essential purpose of therapeutic intervention. Recent studies have reported that CM from dental pulp-derived stem cells has therapeutic benefits. In addition, collagen hydrogel acts as a drug delivery system in SCI experiments. Methods: Stem cells from human exfoliated deciduous teeth (SHEDs) were cultured, and SHED-CM was harvested and concentrated. Collagen hydrogel containing SHED-CM was prepared. The rats were divided into five groups receiving laminectomy, compressive SCI with or without intraspinal injection of biomaterials (SHED-CM), and collagen hydrogel with or without SHED-CM. Basso, Beattie, and Bresnahan (BBB) scoring, inclined plane, cold allodynia, and beam walk tests were performed for 6 weeks to assess locomotor, motor, sensory, and sensory-motor performances, respectively. Results: Scores of the rats receiving SHED-CM loaded in collagen hydrogel were significantly better than those of the other injured groups at 1-week post-injury for BBB, 2 weeks for inclined plane, 2 weeks for cold allodynia, and 4 weeks for beam walk tests (p <0.05). The differences remained significant throughout the study. Conclusions: Intraspinal administration of SHED-CM loaded in collagen hydrogel leads to improved functional recovery and proposes a cell-free therapeutic approach for SCI.

Discoloration of teeth due to different intracanal medicaments

Farzaneh Afkhami,Sadaf Elahy,Alireza Mahmoudi Nahavandi,Mohamadjavad Kharazifard,Aidin Sooratgar 대한치과보존학회 2019 Restorative Dentistry & Endodontics Vol.44 No.1

Objectives: The objective of this study was to assess coronal discoloration induced by the following intracanal medicaments: calcium hydroxide (CH), a mixture of CH paste and chlorhexidine gel (CH/CHX), and triple antibiotic paste (3Mix). Materials and Methods: Seventy extracted single-canal teeth were selected. Access cavities were prepared and each canal was instrumented with a rotary ProTaper system. The specimens were randomly assigned to CH, CH/CHX, and 3Mix paste experimental groups ( n = 20 each) or a control group ( n = 10). Each experimental group was randomly divided into 2 subgroups (A and B). In subgroup A, medicaments were only applied to the root canals, while in subgroup B, the root canals were completely filled with medicaments and a cotton pellet dipped in medicament was also placed in the pulp chamber. Spectrophotometric readings were obtained from the mid-buccal surface of the tooth crowns immediately after placing the medicaments (T1) and at 1 week (T2), 1 month (T3), and 3 months (T4) after filling. The ∆E was then calculated. Data were analyzed using 2-way analysis of variance (ANOVA), 3-way ANOVA, and the Scheffé post hoc test. Results: The greatest color change (ΔE) was observed at 3 months ( p < 0.0001) and in 3Mix subgroup B ( p = 0.0057). No significant color change occurred in the CH ( p = 0.7865) or CH/ CHX ( p = 0.1367) groups over time, but the 3Mix group showed a significant ΔE ( p = 0.0164). Conclusion: Intracanal medicaments may induce tooth discoloration. Use of 3Mix must be short and it must be carefully applied only to the root canals; the access cavity should be thoroughly cleaned afterwards.

Four phase hydrate equilibria of methane and carbon dioxide with heavy hydrate former compounds: Experimental measurements and thermodynamic modeling

Farzaneh Feyzi,Hamed Tavasoli 한국화학공학회 2016 Korean Journal of Chemical Engineering Vol.33 No.8

We experimentally investigated the hydrate dissociation condition for four phase hydrate (H)-aqueous liquid (LAq)-hydrocarbon rich liquid (LHC)-vapor (V) for the ternary systems of help gas-heavy hydrate former-water. Methane and carbon dioxide are known as help gases and benzene and cyclohexane are considered as heavy hydrate formers. The experimental data were generated using an isochoric pressure-search method. Two different equations of state (EOS) were employed to study the equilibrium phase behavior of ternary four phase systems. The EOSs considered are Valderama-Patel-Teja EOS combined with non-density dependent mixing rule (VPT+NDD) and Statistical Associating Fluid Theory EOS proposed by Huang and Radosz (SAFT-HR). The required binary interaction parameters (BIP) were obtained using vapor-liquid equilibrium (VLE) and liquid-liquid equilibrium (LLE) data. The hydrate phase was modeled by the modification of the solid solution theory of van der Waals and Platteeuw. To obtain reliable results, distortion of cages due to occupation of large molecules was considered. The Kihara parameters of cyclohexane were adjusted to hydrate dissociation data. Model calculations for hydrate forming conditions were found to be in satisfactory agreement with the newly reported data in this work and literature data.

Effects of combined elicitors on parthenolide production and expression of parthenolide synthase (TpPTS) in Tanacetum parthenium hairy root culture

Farzaneh Pourianezhad,Hassan Rahnama,Amir Mousavi,Mahmood Khosrowshahli,Sudabeh Mafakheri 한국식물생명공학회 2019 Plant biotechnology reports Vol.13 No.3

Feverfew (Tanacetum parthenium) is a medicinal herb that has multiple pharmacologic properties. This plant is rich parthenolide (PN). Hairy roots (HRs) culture is a valuable biotechnological tool for the production of plant secondary metabolites. Furthermore, application of elicitors is one of the most effective strategies to induce the metabolite biosynthesis. This study has investigated the effects of various elicitors on the production of PN in feverfew hairy root culture and the expression of parthenolide synthase (TpPTS) gene. Three elicitors were used separately and in combination, including yeast extract (YE) (2.5 mgL− 1), methyl jasmonate (MJ) (100 μM), Ag+ (100 μM), YE + MJ, Ag+ + MJ, YE + Ag+, and YE + MJ + Ag+ which were added to the 1/2MS medium on day 18 and after 48 h and 96 h, hairy roots were collected to determine the PN content. Total RNA was extracted from hairy roots and used for cDNA synthesis and real-time PCR analysis to investigate the gene expression of TpPTS. The PN content was determined by HPLC. The highest PN content (0.05 mg g− 1 dry weight) was achieved after application of elicitors (in 2.5 mgL− 1 YE and 100 μM MJ) and the highest expression of TpPTS gene also occurred after 96 h in the same treatment. It was concluded that some of the combined elicitors could efficiently increase the parthenolide content of feverfew in vitro culture. The additive effect of elicitors might be due to the separate mechanisms of each substance that is acting in an independent pathway.

Application of Tumor Markers SCC-Ag, CEA, and TPA in Patients with Cervical Precancerous Lesions

Farzaneh, Farah,Shahghassempour, Shapour,Noshine, Bahram,Arab, Maliheh,Yaseri, Mehdi,Rafizadeh, Mitra,Alizadeh, Kamyab Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.9

Background: To determine the potential clinical utility of tumor markers CEA, TPA, and SCC-Ag for early detection of cervical precancerous lesions. Materials and Methods: A case-control study was carried out on 120 women (46 patients with histologically confirmed cervical precancerous lesions and 74 healthy controls). The significance of serum selected tumor markers in early detection of cervical intraepithelial neoplasia (CIN) were assessed. Results: Of the case group, the rates of CIN I, II, III, was 69.6%, 23.9%, and 6.5%, respectively. According to the manufacturer's cut-off values of 2ng/ml, 5ng/ml, and 70 U/ml for SCC-Ag, CEA and TPA tests, in that order, SCC-Ag test had a sensitivity of 13%, but CEA and TPA tests could not distinguish between case and control groups. The diagnostic sensitivities were highest at cut-off values of 0.55 ng/ml for SCC-Ag, 2.6ng/ml for CEA, and 25.5 U/ml for TPA which were 93%, 61%, and 50%, respectively. However, the area under the receiver operating characteristic curve was the largest for SCC-Ag (0.95 vs. 0.61 and 0.60 for CEA and TPA, respectively). Moreover, there was a highly significant direct correlation between SCC-Ag concentration and the degree of cervical precancerous lesions (r=0.847, p<0.001). Conclusions: The new cutoff of 0.5 for SCC-Ag test might be useful as a tumor marker in Iranian patients with CIN and it needs to be more evaluated by studies with larger populationa.

A Review of Pharmacological Treatments for Vaginal Atrophy in Postmenopausal Women in Iran

Farzaneh Rashidi Fakari,Masoumeh Simbar,Maryam Beheshti Nasab,Masoumeh Ghazanfarpour,Fahimeh Rashidi Fakari 대한폐경학회 2020 대한폐경학회지 Vol.26 No.2

Vaginal atrophy is one of the most common menopausal complications and is often overlooked. There are various pharmacological and non-pharmacological treatment approaches to reduce vaginal atrophy; however, no comprehensive study on a convenient, affordable, inexpensive, and noninvasive treatment with fewer complications has been conducted so far. Thus, the current study aimed to provide a systematic review of pharmacological treatment for vaginal atrophy in postmenopausal women in Iran. In this systematic review, all Iranian articles published in Persian or English during 2009 to 2019 were collected and analyzed by searching the Scopus, PubMed, Web of Science, Magiran, Iranian Registry of Clinical Trials (IRCT), and Cochrane Library databases. The inclusion criteria were clinical trials for vaginal atrophy and menopause. Based on the selection criteria, articles with a Jadad scale score of 3 and above were included in the study and qualitatively analyzed. Overall, 15 clinical trials met the inclusion criteria. In total, 12 articles examined the efficacy of pharmacological treatments (including three herbal medicines, three vitamins and dietary supplements, and two chemical drugs) in treating vaginal atrophy in postmenopausal women. Various types of medication have been used to improve vaginal atrophy, and effective treatments include licorice, chamomile, royal jelly, vitamin E, vitamin D, hyaluronic acid, and Vagifem; however, the results of studies on fennel have been inconsistent. However, considering the small number of studies reviewed, further studies with a stronger methodology are needed to confirm the efficacy of these medications.

Iranian Olive Cultivars Micro Propagation

Farzaneh Tabesh,Maryam Peivandi,Farah Farahani,Seyed Mehdi Hosseini Mazinani 한국원예학회 2006 한국원예학회 기타간행물 Vol.- No.-