자기장 저속 냉동보관법을 이용한 쥐 치아 치주인대세포의 활성도 검사

안현정,김의성,김진,김덕원,김기열,이찬영,이승종 大韓齒科保存學會 2008 Restorative Dentistry & Endodontics Vol.33 No.4

본 연구의 목적은 흰쥐 상악 대구치를 발거하여 자기장 저속 냉동보관법을 이용하여 냉동 시 치주인대세포의 활성도 및 세포 사멸도를 MTT검색법과 TUNEL검사를 이용하여 측정하고자 하였다. 4주령의 암컷 Sprague-Dawley계 된 쥐의 상악 좌우 제1,2대 구치를 발거하여 각군 당 12개의 쥐 치아를MTT검색에 이용하였고 6개의 치아를 TUNEL 검사에 이용하였다. 실험군은 5개군으로 대조군은 즉시 발치군이며 4℃냉장고에서 1주일간 보관한 냉장군, 발치 후 동해방지제 처리과정을 거쳐 -196℃의 액화질소에 넣어 급속 냉동한 액화질소군, 21.7 ㎃, 60 ㎐, 1 G의 자기장을 이용하여 -0.3℃/min 의 속도로 -20℃까지 냉동 후 -196℃로 급속 냉동한 자기장군, -0.3℃/min의 속도로 -20℃까지 냉동 후 -196℃에 급속 냉동한 저속 냉동군으로 나누었다. 보존액은 F medium을 사용했으며 동해방지제로 10% dimethyl sulfoxide (DMSO)를 사용하였다. 치근면을 단위면적으로 표준화하기 위해 MTT측정값을 Eosin 염색 후 530 ㎜에서 측정한 흡광도 값으로 나누었다. TUNEL 검사 시 각 조직슬라이드에서 400배 크기의 현미경 시야에서 임의로 세 부분을 지정하여 정상 세포수와 양성 세포수를 세어 그 비율을 계산하여 각 실험군 당 평균치를 구하였다. 통계 분석을 위해 one way ANOVA를 시행하였으며 사후검정으로 Scheffe와 Tukey HSD방법을 썼으며 결과는 다음과 같다. MTT검색에 의한 흡광도를 Eosin염색 후 측정한 흡광도로 나눈 값에서는 자기장군은 즉시 발치군보다 낮은 세포활성을 보였고 (p < 0.05) 액화질소군, 저속 냉동군과는 통계적으로 유의성 있는 차이를 보이지 않았다. 그러나 자기장군은 액화질소군, 저속 냉동군과 함께 냉장군보다는 높은 세포 활성도를 보였다 (p<0.05).TUNEL검사 결과도 자기장군은 즉시 발치군보다 치주인대의 세포사멸도가 높았으나 (p < 0.05) 저속 냉동군과 액화 질소군과는 통계적으로 유의한 차이를 보이지 않았다. 자기장군은 냉장군보다 세포사멸도가 낮았으며 냉장군은 모든 군 중에서 세포 사멸도가 가장 높았다 (p<0.05). The purpose of this study was to evaluate the viability of periodontal ligament cell in rat teeth using slow cryopreservation method with magnetic field through MTT assay and TUNEL test. For each group, 12 teeth of 4 weeks old white female Sprague-Dawley rat were used for MTT assay, and 6 teeth in TUNEL test. The Maxillary left and right, first and second molars were extracted as atraumatically as possible under tiletamine anesthesia. The experimental groups were groupl (immediately extraction), group 2 (cold preservation at 4℃ for 1 week), group 3 (rapid Cryopreservation in liquid nitrogen), group 4 (slow cryopreservation with magnetic field of 1 G), and group 5 (slow cryopreservation). F medium was used as preservation medium and 10% DMSO as cryoprotectant. After preservation and thawing, the MTT assay and TUNEL test were processed. One way ANOVA and Scheffe method were performed at the 95 % level of confidence. The value of optical density obtained after MTT analysis was divided by the value of eosin staining for tissue volume standardization. In both MTT assay and TUNEL test, it had showed no significant difference among group 3,4, and 5. And group 3 had showed higher viability of periodontal ligament cell than group 2. From this study, slow cryopreservation method with magnetic field can be used as one of cryopreservation methods.

서울시 오존의 통계적 단기예측모형에 관한 연구 : A case of seoul city

김조천,전의찬,전진 동신대학교 환경연구소 2002 환경연구 Vol.7 No.1

본 연구에서는 오존의 당일예보모형 2개(중회귀모형, 판별분석모형)를 선정하고 상호 비교·평가함으로써 모형들이 갖는 특성을 대기오염 관리대책에 반영시키기 위한 연구를 수행하였다. 고농도 오존계절의 분석 결과로부터 4월~8월의 5개월(153일)간의 기간을 광화학스모그 발생가능성이 높은 계절로 선정하였고, 오존농도의 일변화 특성은 오전 8시경부터 점점 증가하다가 오후 2~4시경에 최고점에 달하는 것으로 나타났고, 오존농도의 지역특성을 요인분석을 통하여 분석한 결과, 서울시 전체지역(최소한 반경 15km 이내)의 오존농도 변화특성을 하나의 모형으로 설명하는 데 큰 무리가 없음을 알았다. 모형작성 및 검정·검증을 행하고 상호 비교한 결과, 판별분석 모형이 예상했던 바와 같이 임의의 고농도일 만을 주목한 경우에는 중회귀모형 보다 약간 더 좋은 결과를 보여 주었고, 전반적인 경우에는 두 모형이 비슷한 결과를 나타내었다. Based on of analyzing the season of high level of Ox(Oxidant), five months from April to August were selected. As a result of investigating the weather effect on high level of Ox days and the diurnal variation of Ox, it was observed that the temperature was comparatively high, the wind velocity was very low, and the irradiation intensity was very high. It was found that hourly Ox increased from 8 A.M. and reached its peak by 2~4 P.M. As a result of factor analysis, an area characteristic of Ox could be explained by only one model for the whole area of Seoul(at least within 15 km of a radius). It was proved that a discriminant model is better than a multiple regression model in case that a high level of Ox is concerned, but in general two models were alike. Therefore, in view of photochemical Ox control strategy, it is thought that a discriminant model is more useful than a multiple regression model, in case that the excess of a certain warning criteria is important than any other cases.

鼻毛의 白毛發生에 의한 年齡推定

김진국,홍석찬,진정언,박의우,김동욱 大韓法醫學會 1999 대한법의학회지 Vol.23 No.1

The gray vibrissae of Korean males(n= 189) between 15 and 75 years old and females(n=111) between 15 and 74 years old whop visited the department of otorhinolaryngology, Konkuk university Chungju hospital during 1996 to 1998, were counted for the purpose of estimating approximate age range in individuals of unknown age. The lowest age appearing gray hairs in both sexes was 15-year-old. The Pearson's correlation coefficient in both sexes was 0.490 (p<0.01). The age appearing gray vibrissae in females was slightly earlier than that of males, however, the differences between right and left sides of nasal cavity were insignificant. This method of counting gray vibrissae would be useful to estimate age range in personal identification together with other methods available until now.

農村에 있어서의 腸管系寄生蟲의 豫防對策 三槽式腐敗槽便所의 살아있는 寄生蟲卵 및 病原性腸內細菌의 環境汚染 遮斷效果

金東燦,李溫永,金舜姬,韓義正,金南洙 國立保健硏究院 1977 국립보건연구원보 Vol.14 No.-

고속적응 스펙트럼 추정법의 안정도 개선에 관한 연구

김경렬,정찬수,김의준 崇實大學校 生産技術硏究所 1991 論文集 Vol.21 No.-

In this paper, it is proposed to improve the robustness of the Fast Recursive Least Squares (FRLS) algorithms with the exponential weighting, which is an important class of algorithms for adaptive filtering. It is well known that the FRLS algorithm is numerically unstable with exponential weighting factor λ<1. However, introducing some gains into this algorithms, numerical errors can be reduced. A proper choice of the gains then leads to a numerically stable FRLS algorithm with a complexity of 8m mulitiplications and we shown it by computer simulations.

키토산 구슬을 이용한 가토 연골세포의배양

장의찬,김경환,저호중,이기현,정상인,손태일 대한정형외과연구학회 2003 대한정형외과연구학회지 Vol.6 No.2

하수처리장에서의 악취 배출특성 및 영향 평가

전의찬,사재환,김천두,김조천 동신대학교 환경연구소 2002 환경연구 Vol.7 No.1

본 연구에서는 하수처리장에서 발생되는 악취물질 중의 하나인 암모니아를 대상으로 플럭스와 발생량을 산정하였으며, 대기확산모델을 이용한 하수처리장의 악취 영향 평가를 예측하였다. 열린챔버를 이용한 새료채취에 있어서, 채취시간은 설치 후 60분에 실시하는 것이 가장 적절한 것으로 조사되었다. 하수처리장에서 발생되는 암모니아의 단위시간당 단위면적당 플럭스는 각각 531.7㎍/㎡ㆍsec, 4,925.7 ㎍/㎡ㆍsec로 평가되었으며, 단위시간당 발생량은 각각 평균 17.7 ㎍/sec, 6.3 ㎍/sec으로서 침사지에서 다소 높은 발생속도로 배출되고 있는 것으로 평가되었다. 또한, 배출계수는 평균 114.0 mg/activity-ton, 1,055.6 mg/activity-ton으로 평가되었다. 암모니아에 의한 주변의 영향 평가는 1시간 최대 영향 예측의 결과 부지경계선상에서 악취도 '1.0' 수준으로, 인근마을에는 악취도 '0'에 가까운 수준으로 예측되어 인근 주변에 미치는 영향은 거의 없는 것으로 평가되었다. This study is to estimate the flux and the emission of ammonia, one of regulative odor emitted from the sewage treatment plant, and it is to assess impact of odor on the area near the plant using the atmospheric dispersion model. The flux of ammonia in a grit chamber and SBR reactor of the sewage treatment plant was measured as 17.7㎍/㎡ㆍsec and 6.3㎍/㎡ ㆍsec, respectively. The emission velocity of ammonia from a grit chamber and SBR reactor was investigated as 531.7㎍/sec and 187.9㎍/sec. The emission coefficient was also estimated as average 114.0mg/activity-ton for a grit chamber and 1,056.6 mg/activity-ton for SBR reactor. For the assessment of impact of ammonia on the vicinity of the plant, the odor strength was estimated to '1' or below on the boundary of the plant based on the one-hour maximum odor impact according to the result of simulation of odor dispersion model. As a result of modeling, it was reveled that the impact of odor from the sewage treatment plant was negligible.

환경기초시설 중 하·폐수처리과정으로부터의 GHG 배출량 예측 : 광주·전남지역을 중심으로 Kwang ju·Chollanado

전의찬,김전희,송민종 동신대학교 환경연구소 2000 환경연구 Vol.5 No.1

본 연구는 환경기초시설 중에서 생활하수와 산업폐수 처리장에서 발생하는 특히 광주·전남 지역을 중심으로 온실가스의 배출량과 장래 저감 잠재성을 평가하고, 기후변화협약을 대비한 국가 Inventory의 기초자료로 이용될 수 있도록 하였으며, 결론적으로 하ㆍ폐수부문에서 온실가스 배출 잠재성을 감소시키기 위한 방안으로 먼저 하수 및 폐수의 체계적인 수집이 이루어져야 하며, 수집된 하ㆍ폐수는 반드시 적절한 처리과정을 통하여 수계에 배출되어져야 한다. 또한 적절한 처리과정 중에 배출되는 온실기체의 대기 중으로의 누설을 억제하고 회수율을 높이는 관리대책이 수립되어야 하며, 회수율을 높이는 대책은 하ㆍ폐수부문에서 온실가스 배출 잠재력에 대한 저감율에 대하여 좀더 효율적임을 알 수 있다. 또한 상기와 같은 저감방법 외에도 하ㆍ폐수부문에서의 온실가스 저감잠재력을 위하여 정부는 온실가스 배출량을 계산하는데 필요한 여러 가지 자료를 확보하고, 좀더 신뢰할 수 있는 온실가스 배출량을 산출하며, 이들 자료를 토대로 정부는 하ㆍ폐수부문에서의 온실가스 저감잠재력을 위하여 연차적으로 관리대책을 설정해야 한다. 그리고 이에 대한 검중체계를 마련하여 전ㆍ후 효과분석을 해야 할 것이다. This study evaluated greenhouse gas(GHG) emissions and future reduction potential emitted from domestic and industrial wastewater treatment system in Kwangju city and Chollanamdo. For decreasing of GHG emissions potential, systematic collection method of domestic and industrial wastewate is established and collected domestic and industrial wastewater is treated reasonal wastewater treatment process. GHG which is leak from wastewater treatment process to air was controled, Control policy which are increasing recovery ratio was established. In addition to decreasing method, Government make sure of variable data fro estimating GHG emissions quality and evaluated GHG emissions quality reliable method.

이산화티타늄계 광촉매를 이용한 톨루엔의 처리 특성

전의찬,송민종,김전희 동신대학교 환경연구소 2000 환경연구 Vol.5 No.1

각종 급ㆍ만성의 건강장해를 일으킬 뿐만 아니라, 광화학 반응에 의해 오존을 형성하는 것으로 알려진 휘발성유기화합물 중 대표적인 물질인 톨루엔을 광촉매산화법으로 처리할 경우 최적 처리조건을 분석하고자 광촉매의 결정구조, 광원의 세기, 전자수용체로서 산소의 유무 등에 따른 톨루엔의 처리효율을 분석하였다. 실험결과 루틸령 결정구조에 비해 아나타제형 결정구조를 갖는 이산화티타늄의 톨루엔 처리효율이 높게 나타났으며, 광원의 세기가 높을수록 전자수용체로서 산소가 존재할 경우 높은 처리효율을 나타내었다. 시간과 공간적인 제약으로 물, 오존 그리고 증기와 같은 다양한 전자수용체가 존재할 경우의 톨루엔의 처리효율을 파악하지 못하였으며, 온도, 습도 및 촉매활성에 영향을 주는 여러 가지 인자들을 대상으로 그것들의 변화에 따른 처리효율의 고찰을 못하였다. 앞으로 보다 효율적인 이산화티타늄계 광촉매의 제조방법 개발과 이산화티타늄계 광촉매를 이용한 다양한 VOC 처리방법의 개발이 요구된다. An available methods for abating VOC(including Toluene) are thermal or catalytic incineration, liquid absorption, solid adsorption, condensing, membranes, biodegradation and plasma discharge, but a number of defects are these VOC abating methods, including ; requires a auxiliary fuel, generate secondary pollutants(waste catalysts, waste absorbents, waste adsorbents, ozone, etc.), a large scale, etc.. But photoactive catalysts, when illuminated with UV-light, generate highly reactive radicals that can oxidize the organic contaminants in gas. One methods to increase the efficiency of the process, and thereby reduce the light energy requirement, is by developing more active catalysts. Because of, ball type TiO2 catalyst were obtained commercially and/or prepared in laboratory were examined for their photoacivity, and they are TiO2 added some annex(Al203, SiO2, KsO, P205, etc.). The organic compounds used to identify the best photocatalysis condition were toluene. This study also examined the impact of catalyst structure, light intensity, existence of oxygen as an electron acceptor and Toluene initial concentration on the reaction kinetics. Labratory experiments were performed to investigate TiO2 photocataysis for treating test gas contaminated with Toluene. and TiO2 photocatalysis efficiency for mineralization of Toluene is also evaluated. The photooxidation of Toluene was carried out in a photocatalytic reactor at room temperature. I performed Toluene photocatalytic oxidation experiments using a single-pass annular reactor. The organic-contaminated gas was prepared in teflon sampling bag(25L) and passing through photocatalytic reactor by vacuum pump which is located reactor outlet. The Toluene diluted with mixture air or nitrogen gas. Flow rates for test gas stream were controlled by micro flow meter going out the reactor. The reactor illuminated with 15W near-UV lamp, BLB lamp or Fluorescent lamp. The light intensity at the catalyst surface were estimated to be 0.8 to 6㎽ ㎝-2s-1. The inner cell of the reactor was packed with ball type titanium dioxide(φ 6㎜). Toluene was determined by GC-FID of gas samples taken from the a glass sampling bulb which was located at reactor outlet by gas-tight syringe. TiO2 photocataysis for treating test gas contaminated with Toluene. and TiO2 photocatalysis efficiency for mineralization of Toluene is also evaluated. Using the anatase type photocatalyst, Touene conversion rate is greater than that of rutile(86.1% conversion of 100ppmv Toluene using anatase type photocatalyst, 78% conversion of 100ppmv Touene using rutile type photocatalyst at 0.5L/min) Effect of light intensity on the rate of Toluene photocatalytic decomposition was investigated by three type lamps within the reactor. When light intensity to increase, the Toluene conversion rate to increase. Using the BLB lamp showed higher activity than the Fluorescent lamp, but UV lamp showed the hight photocatalytic activity(19.1% conversion of 100ppmv Toluene with Fluorescent lamp, 60.4% conversion of 100ppmv Toluene with BLB lamp, 86.1% conversion of 100ppmv Toluene with UV lamp at 0.5L/min). Experiments with added oxygen as an electron acceptor improved the reaction rate considerably(86.1% conversion of 100ppmv Toluene without oxygen, 62.5% conversion of same Toluene concentration with mixture air at 0.5L/min).

이산화티타늄계 광촉매를 이용한 벤젠의 처리 특성

전의찬,송민종,김전희 동신대학교 환경연구소 1999 환경연구 Vol.4 No.1

An available methods for abating VOC(including Benzene) are thermal or catalytic incineration, liquid absorption, solid adsorption, condensing, membranes, biodegradation and plasma discharge, but a number of defects are these VOC abating methods, including ; requires a auxiliary fuel, generate secondary pollutants(waste catalysts, waste absorbents, waste adsorbents, ozone, etc.), a large scale, etc.. But photoactive catalysts, when illuminated with UV-light, generate highly reactive radicals that can oxidize the organic contaminants in gas. One methods to increase the efficiency of the process, and thereby reduce the light energy requirement, is by developing more active catalysts. Because of, ball type TiO₂ catalyst were obtained commercially and/or prepared in laboratory were examined for their photoacivity, and they are TiO₂ added some annex(Al₂O₃, SiO₂, KsO, P₂O_(5), etc.). The organic compounds used to identify the best photo catalysis condition were benzene. This study also examined the impact of catalyst structure, light intensity, existence of oxygen as an electron acceptor and Benzene initial concentration on the reaction kinetics. Labratory experiments were performed to investigate TiO₂ photo catalysis for treating test gas contaminated with Benzene. and TiO₂ photo catalysis efficiency for mineralization of Benzene is also evaluated. The photo oxidation of Benzene was carried out in a photo catalytic reactor at room temperature. I performed BTX photo catalytic oxidation experiments using a single-pass annular reactor. The organic-contaminated gas was prepared in Teflon sampling bag(25L) and passing through photo catalytic reactor by vacuum pump which is located reactor outlet. The BTX diluted with mixture air or nitrogen gas. Flow rates for test gas stream were controlled by micro flow meter going out the reactor. The reactor illuminated with 15W near-UV lamp, BLB lamp or Fluorescent lamp. The light intensity at the catalyst surface were estimated to be 0.8 to 6mW cm-2s-1. The inner cell of the reactor was packed with ball type titanium dioxide(Ø 6mm). Benzene was determined by GC-FID of gas samples taken from the a glass sampling bulb which was located at reactor outlet by gas-tight syringe. TiO₂ photo catalysis for treating test gas contaminated with Benzene. and TiO₂ photo catalysis efficiency for mineralization of Benzene is also evaluated. Using the anatase type photo catalyst, Benzene conversion rate is greater than that of rutile(92% conversion of 100ppmv Benzene using anatase type photo catalyst, 80% conversion of 100ppmv Benzene using rutile type photo catalyst at 0.5L/min) Effect of light intensity on the rate of Benzene photo catalytic decomposition was investigated by three type lamps within the reactor. When light intensity to increase, the Benzene conversion rate to increase. Using the BLB lamp showed higher activity than the Fluorescent lamp, but UV lamp showed the hight photo catalytic activity(26% conversion of 100ppmv Benzene with Fluorescent lamp, 63.5% conversion of 100ppmv Benzene with BLB lamp, 92% conversion of 100ppmv Benzene with UV lamp at 0.5L/min). Experiments with added oxygen as an electron acceptor improved the reaction rate considerably(92% conversion of 100ppmv Benzene without oxygen, 66.1% conversion of same Benzene concentration with mixture air at 0.5L/min). The Benzene conversion rate begins to decrease while Benzene initial concentration begins to increase. We have thus concentrated our effects on the study of Benzene degradation kinetics in vary initial Benzene concentration.