UPLC를 이용한 밀크씨슬추출물 지표 성분인 실리마린 분석법 검증

유동선 ( Dongsun Yoo ),정경희 ( Kyung Hee Jung ),최승준 ( Seung Jun Choi ) 한국산업식품공학회 2018 산업 식품공학 Vol.22 No.2

This study attempted to establish an ultra performance liquid chromatography (UPLC) analysis method for standard determination of silymarin as a health functional food material in Silybum marianum extraxt (milk thistle). UPLC was performed on a Waters Acquity BEH C18 (50×2.1 mm, 1.7 μm) column using a gradient elution of distilled water and methanol at a flow rate of 0.21 mL/min and detection wavelength of 288 nm. The UPLC method showed high linearity in the calibration curve at a coefficient of determination (r2) of >0.9999, and limit of detection and quantitation for 6 flavonolignans were 0.0167-0.2469 and 0.1648-1.2931 μg/mL, respectively. The recovery of each flavnolignan was in the range of 99.96-100.81%, and the relative standard deviation for precision of each flavonolignan was less than 1.0%. The UPLC method established in this study was more specific for the quantitative determination of silymarin than the HPLC method. Also, since the UPLC method is shorter in the equipment operation time and smaller in the amount of used solvent than the HPLC method, UPLC is expected to have higher energy efficiency and lower environmental impact compared with HPLC.

SATEEC 시스템을 이용한 객토 토양의 토성고려에 따른 도암댐 유역의 토양유실 및 유사량 분석

유동선 ( Dongsun Yoo ),안재훈 ( Jaehun Ahn ),윤정숙 ( Jongsuk Yoon ),허성구 ( Sunggu Heo ),박윤식 ( Younshik Park ),김종건 ( Jonggun Kim ),임경재 ( Kyoung Jae Lim ),김기성 ( Ki-sung Kim ) 한국농공학회 2007 한국농공학회 학술대회초록집 Vol.2007 No.-

There have been serious soil erosion and water pollution problems caused by highland agriculture practices at Doam-dam watershed. Especially agricultural activities, chemical and organic fertilizer and pesticide applications, soil reconditioning to maintain soil fertility are known as primary causes of soil erosion and water qaulity degradation in the receiving water bodies. Among these, soil reconditioning can accelerate soil erosion rates. To develop soil erosion prevention practices, it is necessary to first estimate the soil erosion from the watershed. Thus, the Universal Soil Loss Equation (USLE) model has been developed and utilized to assess soil erosion. However, the USLE model cannot be used at watershed scale because it does not consider sediment delivery ratio (SDR) for watershed application. For this reason, the Sediment Assessment Tool for Effective Erosion Control (SATEEC) was developed to assess the sediment yield at any point in the watershed. The USLE-based SATEEC system can estimate the SDR using area-based SDR and slope-based SDR module. In this study, the SATEEC system was used to estimate soil erosion and sediment yield at the Doam-dam watershed using the soil properties from reconditioned agricultural fields. Based on the soil sampling and analysis, the USLE K factor was calculated and used in the SATEEC system to analyze the possible errors of previous USLE application studies using soil properties from the digital soil map, compared with that using soil properties obtained in this study. The estimated soil erosion at the Doam-dam watershed without using soil properties obtained in the soil sampling and analysis is 1,791,400 ton/year (123 ton/ha/year), while the soil erosion amounts is 2,429,900 ton/year (166.8 ton/ha/year) with the use of soil properties from the soil sampling and analysis. There is 35% increases in estimated soil erosion and sediment yield with the use of soil properties from soil reconditioned agricultural fields. Since significant amounts of soil erosion are known to be occurring from the agricultural fields, the soil erosion and sediment yield from only agricultural fields was assessed. The soil erosion rate is 45.9 ton/ha/year without considering soil properties from soil reconditioned agricultural fields, while 105.3 ton/ha/year after considering soil properties obtained in this study, increased in 129%. This study shows it is very important to use correct soil properties to assess soil erosion and sediment yield simulation. It is recommended that further studies are needed to develop environment friendly soil reconditioning method should be developed and implemented to decrease the speed of soil erosion rates and water quality degradation.

A Dunnione Compound MB12662 Improves Cisplatin-Induced Tissue Injury and Emesis

( Dongsun Park ),( In Geun Jo ),( Ja Young Jang ),( Tae Hwan Kwak ),( Sang Ku Yoo ),( Jeong Hee Jeon ),( Ehn-kyoung Choi ),( Seong Soo Joo ),( Okjin Kim ),( Yun-bae Kim ) 한국응용약물학회 2015 Biomolecules & Therapeutics(구 응용약물학회지) Vol.23 No.5

The present study was aimed to investigate the effects of MB12662, a synthetic dunnione compound, on cisplatin-induced vomiting reflexes and intestinal, renal, immune system, and hematopoietic toxicities in ferrets and mice, respectively. Male ICR mice were orally administered MB12662 (5, 10, 25 or 50 mg/kg) for 10 days, during which intraperitoneally challenged with cisplatin (3.5 mg/kg) from day 4 to 7, and sacrificed on day 10 for the pathological examination. Male ferrets were orally administered MB12662 (25, 50 or 100 mg/kg) for 7 days, subcutaneously challenged with cisplatin (5 mg/kg), and monitored for vomiting reflexes and survival of the animals. Four-day injection of cisplatin (3.5 mg/kg) to mice caused body weight loss and degeneration and atrophy of intestinal villi, reducing villi/crypt ratio to a half level of control animals. Cisplatin also induced renal and hepatic toxicities, and depletion of splenocytes and bone marrow progenitor cells. The systemic toxicities including decreased villi/crypt ratio, immune system atrophy, splenocyte depletion, and decreased cellularity in bone marrow were improved by MB12662. Cisplatin (5 mg/kg) induced retching and emetic responses of ferrets, which were remarkably attenuated by MB12662 in a dose-dependent manner. All the ferrets pretreated with MB12662 survived the challenge of cisplatin, in comparison with 40% mortality in vehicle-treated animals, and blood parameters of nephrotoxicity and hepatotoxicity were markedly recovered. It is expected that MB12662 could be a candidate for the body protection against burden, including emesis, of chemotherapeutic agents.

Diagnostic Accuracy of Working Memory Tasks Depending on Scoring Unit and Condition in Preschool Children

임동선(Dongsun Yim),김신영(Shinyoung Kim),유지원(Jeewon Yoo),이윤정(Yunjung Lee),이상언(Sangeon Lee),정하은(Haeun Chung) 한국언어청각임상학회 2017 Communication Sciences and Disorders Vol.22 No.3

배경 및 목적: 비단어따라말하기, 문장따라말하기, 매트릭스는 언어능력의 기저요인으로 주목 받아온 작업기억의 각 하위체계를 평가하기 위한 과제로, 많은 선행연구들과 임상에서 사용되어 왔다. 그러나 각 과제의 채점단위 또는 수행조건이 일치되지 않아왔던 바, 본 연구에서는 각 과제별로 언어장애를 보다 정확하고 효과적으로 판별할 수 있는 방식을 재검토하고 정확한 시행방법을 제시하고자 한다. 방법: 본 연구는 5-6세의 어휘발달지체 아동(N=40)과 일반 아동(N=40)을 대상으로 비단어따라말하기 및 문장따라말하기의 채점단위 및 매트릭스 과제의 수행조건에 따른 진단정확도를 민감도, 특이도, 양성우도비 및 음성우도비를 통하여 분석하였다. 결과: 판별분석 결과, 비단어따라말하기 과제는 음절 단위 채점방식에 비해 낱말 단위 채점방식이, 문장따라말하기 과제는 어절 단위 채점방식에 비해 문장 단위 채점방식의 진단정확도가 더욱 높은 것으로 나타났다. 매트릭스 과제는 집단 간 수행력 차이와 판별분석 결과가 순행 및 역행 조건 모두에서 유의하지 않았다. 논의 및 결론: 비단어따라말하기 과제 및 문장따라말하기 과제는 시간적 효율성이 높은 채점 방식이 진단정확도도 높은 것으로 나타나 그 임상적 효용성을 입증하였다. Objectives: The purpose of the current study was to examine the diagnostic accuracy of working memory (WM) tasks, including nonword repetition (NWR), sentence repetition (SR), and matrix tasks, as clinically efficient diagnostic tools depending on the scoring systems and task condition. Methods: Forty children with vocabulary delay (VD group) and 40 typically developing children (TD group) were administered a series of WM tasks (NWR, SR, and matrix tasks). Different scoring units were applied to each task (syllable vs. word for NWR task and word vs. sentence for SR task) while different conditions (forward matrix vs. backward matrix) were used for the matrix task. Separate discriminant function analyses were conducted with the scores calculated by the different scoring units or the different condition of the tasks as predictors. Results: Multiple discriminant function analyses for the NWR task yielded word units with higher overall classification values than syllable units, whereas for the SR task sentence units had a higher overall classification value than word units. For the matrix task, none of the conditions reached the level of significance in the discriminant function analyses. Conclusion: The results in this study support the diagnostic accuracy of the NWR task with word units and the SR task with sentence units in discriminating among the VD group and the TD group. However, caution should be used when regarding these matrix tasks as an accurate diagnostic tool, especially in young children.

A Dunnione Compound MB12662 Improves Cisplatin-Induced Tissue Injury and Emesis

Park, Dongsun,Jo, In Geun,Jang, Ja Young,Kwak, Tae Hwan,Yoo, Sang Ku,Jeon, Jeong Hee,Choi, Ehn-Kyoung,Joo, Seong Soo,Kim, Okjin,Kim, Yun-Bae The Korean Society of Applied Pharmacology 2015 Biomolecules & Therapeutics(구 응용약물학회지) Vol.23 No.5

The present study was aimed to investigate the effects of MB12662, a synthetic dunnione compound, on cisplatin-induced vomiting reflexes and intestinal, renal, immune system, and hematopoietic toxicities in ferrets and mice, respectively. Male ICR mice were orally administered MB12662 (5, 10, 25 or 50 mg/kg) for 10 days, during which intraperitoneally challenged with cisplatin (3.5 mg/kg) from day 4 to 7, and sacrificed on day 10 for the pathological examination. Male ferrets were orally administered MB12662 (25, 50 or 100 mg/kg) for 7 days, subcutaneously challenged with cisplatin (5 mg/kg), and monitored for vomiting reflexes and survival of the animals. Four-day injection of cisplatin (3.5 mg/kg) to mice caused body weight loss and degeneration and atrophy of intestinal villi, reducing villi/crypt ratio to a half level of control animals. Cisplatin also induced renal and hepatic toxicities, and depletion of splenocytes and bone marrow progenitor cells. The systemic toxicities including decreased villi/crypt ratio, immune system atrophy, splenocyte depletion, and decreased cellularity in bone marrow were improved by MB12662. Cisplatin (5 mg/kg) induced retching and emetic responses of ferrets, which were remarkably attenuated by MB12662 in a dose-dependent manner. All the ferrets pretreated with MB12662 survived the challenge of cisplatin, in comparison with 40% mortality in vehicle-treated animals, and blood parameters of nephrotoxicity and hepatotoxicity were markedly recovered. It is expected that MB12662 could be a candidate for the body protection against burden, including emesis, of chemotherapeutic agents.

딥러닝 기반 Depth Estimation 활용을 통한 MONO SLAM 위치 추정 성능 개선 연구

임동선(Dongsun Lim),김진관(Jingwan Kim),유진우(Jinwoo Yoo) 한국자동차공학회 2022 한국자동차공학회 부문종합 학술대회 Vol.2022 No.6

All-atom simulation of molecular orientation in vapor-deposited organic light-emitting diodes

Youn, Yong,Yoo, Dongsun,Song, Hochul,Kang, Youngho,Kim, Kye Yeop,Jeon, Sang Ho,Cho, Youngmi,Chae, Kyungchan,Han, Seungwu The Royal Society of Chemistry 2018 Journal of Materials Chemistry C Vol.6 No.5

<P>Molecular orientation in vapor-deposited organic semiconductor films is known to improve the optical and electrical efficiencies of organic light-emitting diodes, but atomistic understanding is still incomplete. In this study, using all-atom simulation of vapor deposition, we theoretically investigate how the molecular orientation depends on various factors such as the substrate temperature, molecular shape, and material composition. The simulation results are in good agreement with experiment, indicating that the all-atom simulation can predict the molecular orientation reliably. From the detailed analysis of the dynamics of molecules, we suggest that the kinetics of molecules near the surface mainly determines the orientation of the deposited film. In addition, the oriented films have higher density and thermal stability than randomly oriented films. We also show that higher mobility of laterally oriented films can be explained in terms of the site-energy correlation.</P>

학령기 어휘취약아동 및 또래 아동의 집행기능과 어휘 및 읽기능력과의 관계

유지원(Jeewon Yoo),임동선(Dongsun Yim) 한국언어청각임상학회 2018 Communication Sciences and Disorders Vol.23 No.3

배경 및 목적: 다양한 언어장애 집단의 집행기능과 언어능력과의 관계는 잘 알려져 있으나, 어휘지체 아동의 집행기능과 언어능력과의 관계에 관한 연구는 찾아보기 힘들다. 본 연구에서는 학령기 일반아동과 어휘취약 아동의 집행기능을 비교하고 집행기능의 3가지 구성요소와 읽기 및 어휘를 포함한 언어능력과의 연결관계를 살펴보았다. 방법: 본 연구는 7-9세의 어휘취약 아동(15명)과 일반아동(20 명)을 대상으로 작업기억 억제능력 전환능력을 포함한 언어적/비언어적 집행기능 과제를 실시하고, 규준화된 어휘와 읽기 과제를 시행 하였다. 결과: 일반아동은 digit n-back 과제에서 어휘취약 아동보다 더 높은 정확도를 보였으며 나머지 집행기능 과제에서는 두 그룹이 비슷한 수행능력을 보였다. 일반아동은 언어적 작업기억과 어휘 및 읽기능력 사이에 그리고 전환능력과 어휘 사이에 유의미한 상관관 계가 관찰된 반면, 어휘지체 아동들에게서는 이러한 관계가 관찰되지 않았다. 논의 및 결론: 이 결과는 두 그룹 사이에 집행기능과 언어능력 사이에 다른 상관관계를 보여준다. 즉, 일반아동의 경우에만 집행기능과 어휘 및 읽기와 같은 언어능력 사이에 긴밀한 연결관계를 보여주며, 어휘취약 아동의 경우 언어가 관련된 능력에 집행기능이 효과적으로 작동되지 않음을 시사한다 Objectives: We aimed to compare the executive function (EF) skills between children with typical development (TD) and children with poor vocabulary (PV), and to explore how and what kinds of connections exist between the three components of the EF system and language abilities, including vocabulary knowledge and reading ability, in two groups. Methods: Twenty children with TD and 15 children with PV were assessed via tests of working memory, inhibition, and shifting in both verbal and nonverbal modalities, and standardized vocabulary and reading tests were also administered. Results: Comparisons between the two groups revealed that both groups performed comparably on all EF tasks except for the digit n-back task, with the TD group having a higher accuracy than the PV group. Correlational analyses revealed the significant relationships between language abilities (reading and vocabulary knowledge) and verbal working memory as well as between vocabulary knowledge and shifting for the TD group, whereas no relationship between language abilities and EF was found in the PV group. Conclusion: These findings highlight the different relationships between EF and language abilities in two groups, while showing close links between language abilities and EF only for the TD group. This suggests that EF systems may not be efficiently involved in language related abilities in children with PV.

동적 환경에서 SLAM 위치 추정의 성능 향상을 위한 객체 Movement 인식 방법

김진관(Jingwan Kim),임동선(Dongsun Lim),유진우(Jinwoo Yoo) 한국자동차공학회 2021 한국자동차공학회 학술대회 및 전시회 Vol.2021 No.11

Effects of β-Lapachone on Gastric Secretion

Yun-Bae Kim, Dongsun Park, In Geun Cho, Yun-Hui Yang, Jangbeen Kyung, Dajeong Kim, Ehn-Kyoung Choi, Tae Hwan Kwak3, Sang Ku Yoo 충북대학교 동물의학연구소 2011 Journal of Biomedical and Translational Research Vol.12 No.3

The effects of β-lapachone on gastric secretion were investigated. The pylorus of male Sprague-Dawley rats was ligated and intraduodenally injected with β-lapachone, and the volume, pH, free HCl, and total acidity of gastric fluid were measured 6 hours after the operation. Treatment with β-lapachone resulted in dose-dependent inhibition of gastric secretion Gastric fluid was reduced to 42.9% of control level by 100 mg/kg of β-lapachone, leading to an increase of pH to 6.70 from 1.85 in the control group. In parallel with the increase of pH, at this dosage, free HCl and total acidity decreased to 16.7% and 12.0%, respectively, of control levels. β-Lapachone exhibited ED50 values of 72, 46, and 47 mg/kg for inhibition of gastric volume, free HCl, and total acidity, respectively, implying a superior efficacy on gastric acid to volume. In comparison, pantoprazole (30 mg/kg) reduced the volume, free HCl and total acidity of gastric fluid to 53.0%, 26.0%, and 25.0%, respectively, of control levels, resulting in an increase in pH to 6.36. In the current study, it was confirmed that β-lapachone at an appropriate dose (100 mg/kg) exerted a higher inhibitory effect on gastric secretion than pantoprazole (30 mg/kg), a well-known proton-pump inhibitor. Therefore, it is suggested that β-lapachone could be a candidate compound for prevention or treatment of gastric ulcers induced by diverse psychological and physical stimuli.