Protective Effects of Green Tea Polyphenol Against Renal Injury Through ROS-Mediated JNK-MAPK Pathway in Lead Exposed Rats

Wei Li,Haidong Wang,Deyuan Li,Zhongze Hu,Siming Zhao,Zhejun Zheng 한국분자세포생물학회 2016 Molecules and cells Vol.39 No.6

To investigate the potential therapeutic effects of polyphenols in treating Pb induced renal dysfunction and intoxication and to explore the detailed underlying mechanisms. Wistar rats were divided into four groups: control groups (CT), Pb exposure groups (Pb), Pb plus Polyphenols groups (Pb+PP) and Polyphenols groups (PP). Animals were kept for 60 days and sacrificed for tests of urea, serum blood urea nitrogen (BUN) and creatinine. Histological evaluations were then performed. In vitro studies were performed using primary kidney mesangial cells to reveal detailed mechanisms. Cell counting kit-8 (CCK-8) was used to evaluate cell viability. Pb induced cell apoptosis was measured by flow cytometry. Reactive oxygen species (ROS) generation and scavenging were tested by DCFH-DA. Expression level of tumor necrosis factor- (TNF-), interleukin-1- (IL-1-) and IL-6 were assayed by ELISA. Western blot and qPCR were used to measure the expression of ERK1/2, JNK1/2 and p38. Polyphenols have obvious protective effects on Pb induced renal dysfunction and intoxication both in vivo and in vitro. Polyphenols reduced Pb concentration and accumulation in kidney. Polyphenols also protected kidney mesangial cells from Pb induced apoptosis. Polyphenols scavenged Pb in-duced ROS generation and suppressed ROS-mediated ERK/JNK/p38 pathway. Downstream pro-inflammatory cytokines were inhibited in consistency. Polyphenol is protective in Pb induced renal intoxication and inflammatory responses. The underlying mechanisms lie on the antioxidant activity and ROS scavenging activity of polyphenols.

Resistive switching behavior and mechanism of room-temperaturefabricated flexible Al/TiS2-PVP/ITO/PET memory devices

Deyuan Lyu,Cong Hu,Yuting Jiang,Na Bai,Qi Wang,Deyan He,Jing Qi,Yingtao Li 한국물리학회 2019 Current Applied Physics Vol.19 No.4

The mixture of two-dimensional (2D) TiS2 nanoflakes and polyvinylpyrrolidone (PVP) exhibits a nonvolatile, bipolar resistive switching behavior with a low resistance state (LRS)/high resistance state (HRS) current ratio of ∼102 in the devices with a flexible Al/TiS2-PVP/indium tin oxide (ITO)/polyethylene terephthalate (PET) structure. The polymer-assistant liquid-phase exfoliation of 2D nanoflakes from TiS2 bulk material is processed in low-boiling solvent. And the fabrication process of these devices is performed entirely at room temperature. Such an energy-saving and scalable production process indicates a huge potential of large-scale industrial application. The AFM and TEM characterizations showed that the exfoliated 2D TiS2 are flakes at micrometer scale with a layer-number of mostly 7 or 8. Both the HRS and the LRS can be kept for more than 104 s. The endurance of devices was obtained over 100 direct current (DC) sweeping cycles with remarkable separations between different resistive states. The distributions of writing (set) and erasing (reset) voltages show that set and reset voltages are small (< 2 V). Also, the resistive switching characteristics of the devices are stable during 1000 bending cycles. The switching behavior is explained by the thinning and recovery of Schottky barriers within devices.

Positive effects of porcine IL-2 and IL-4 on virus-specific immune responses induced by the porcine reproductive and respiratory syndrome virus (PRRSV) ORF5 DNA vaccine in swine

Deyuan Tang,Jian Liu,Chunyan Li,Hua Zhang,Ping Ma,Xianfeng Luo,Zhiyong Zeng,Nining Hong,Xia Liu,Bin Wang,Feng Wang,Zhenlei Gan,Fei Hao 대한수의학회 2014 JOURNAL OF VETERINARY SCIENCE Vol.15 No.1

The purpose of this study was to investigate the effects ofporcine interleukin (IL)-2 and IL-4 genes on enhancing theimmunogenicity of a porcine reproductive and respiratorysyndrome virus ORF5 DNA vaccine in piglets. Eukaryoticexpression plasmids pcDNA-ORF5, pcDNA-IL-2, andpcDNA-IL-4 were constructed and then expressed in Marc-145cells. The effects of these genes were detected using an indirectimmunofluorescent assay and reverse transcriptionpolymerase chain reaction (RT-PCR). Characteristicfluorescence was observed at different times after pcDNAORF5was expressed in the Marc-145 cells, and PCR productscorresponding to ORF5, IL-2, and IL-4 genes were detected at48 h. Based on these data, healthy piglets were injectedintramuscularly with different combinations of the purifiedplasmids: pcDNA-ORF5 alone, pcDNA-ORF5 + pcDNA-IL-2,pcDNA-ORF5 + pcDNA-IL-4, and pcDNA-ORF5 + pcDNAIL-4 + pcDNA-IL-2. The ensuing humoral immune responses,percentages of CD4+ and CD8+ T lymphocytes, proliferationindices, and interferon-γ expression were analyzed. Resultsrevealed that the piglets co-immunized with pcDNA-ORF5 +pcDNA-IL-4 + pcDNA-IL-2 plasmids developed significantlyhigher antibody titers and neutralizing antibody levels, hadsignificantly increased levels of specific T lymphocyteproliferation, elevated percentages of CD4+ and CD8+ Tlymphocytes, and significantly higher IFN-γ production thanthe other inoculated pigs (p < 0.05).

Protective Effects of Green Tea Polyphenol Against Renal Injury Through ROS-Mediated JNK-MAPK Pathway in Lead Exposed Rats

Wang, Haidong,Li, Deyuan,Hu, Zhongze,Zhao, Siming,Zheng, Zhejun,Li, Wei Korean Society for Molecular and Cellular Biology 2016 Molecules and cells Vol.39 No.6

To investigate the potential therapeutic effects of polyphenols in treating Pb induced renal dysfunction and intoxication and to explore the detailed underlying mechanisms. Wistar rats were divided into four groups: control groups (CT), Pb exposure groups (Pb), Pb plus Polyphenols groups (Pb+PP) and Polyphenols groups (PP). Animals were kept for 60 days and sacrificed for tests of urea, serum blood urea nitrogen (BUN) and creatinine. Histological evaluations were then performed. In vitro studies were performed using primary kidney mesangial cells to reveal detailed mechanisms. Cell counting kit-8 (CCK-8) was used to evaluate cell viability. Pb induced cell apoptosis was measured by flow cytometry. Reactive oxygen species (ROS) generation and scavenging were tested by DCFH-DA. Expression level of tumor necrosis factor-${\alpha}$ (TNF-${\alpha}$), interleukin-1-${\beta}$ (IL-1-${\beta}$) and IL-6 were assayed by ELISA. Western blot and qPCR were used to measure the expression of ERK1/2, JNK1/2 and p38. Polyphenols have obvious protective effects on Pb induced renal dysfunction and intoxication both in vivo and in vitro. Polyphenols reduced Pb concentration and accumulation in kidney. Polyphenols also protected kidney mesangial cells from Pb induced apoptosis. Polyphenols scavenged Pb induced ROS generation and suppressed ROS-mediated ERK/JNK/p38 pathway. Downstream pro-inflammatory cytokines were inhibited in consistency. Polyphenol is protective in Pb induced renal intoxication and inflammatory responses. The underlying mechanisms lie on the antioxidant activity and ROS scavenging activity of polyphenols.

Reduction of Erythrocyte Fluid Adaptability Due to Cell Membrane Hardening Based on Single‑Cell Analysis

Yuanyuan Chen,Zhongnan Li,Xue Bai,Yanmin Feng,Lin Feng,Deyuan Zhang,Huawei Chen,Haosheng Chen 한국바이오칩학회 2021 BioChip Journal Vol.15 No.1

Because of the rapid development of precision medicine, single-cell analysis has attracted increasing research attention, especially for erythrocyte, whose potential role in the formation of vascular plaque (atherosclerosis) has emphasized the importance of flow characteristics of single erythrocytes in bionic microfluidics. Based on the high incidence of vascular plaques among the elderly and those who have received blood transfusions, we hypothesized that cell membrane hardening changes the fluid adaptability of individual erythrocytes. This hypothesis was verified using an in vitro microfluidic technique based on an analysis of the flow morphology and cell trajectory of individual cells. A symmetrical microchannel was fabricated with a central stenosis to simulate a blood vessel containing plaque. During flowing through this microchannel, normal erythrocyte predominantly exhibited deforming, rotating, and lifting morphologies, resulting in discontinuous contact with the channel wall and a narrower distribution. Conversely, hardened erythrocytes exhibited rolling, swinging, and tumbling morphologies, resulting in stable and continuous contact with the channel wall and a wider distribution. These results indicate that cell membrane hardening decrease cell fluid adaptability on a microscopic scale. This research can offer some new insights into vascular plaques research from a bio-tribological and mechanical perspectives.