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Deng Li,Yong Kang,Xiaolong Ding,Xiao-Chuan Wang,Wenchuan Liu 대한기계학회 2017 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.31 No.3
Self-excited oscillation pulsed waterjet (SOPW) can take advantage of water hammer effect by generating discrete water slugs with large pressure oscillations. To further improve its efficiency, the performance of a jet-driven Helmholtz oscillator generating SOPWs was experimentally studied with feeding pipe diameter being considered. The axial pressure oscillations of the jets against the chamber length and standoff distance under four pump pressures were analyzed. It was found that feeding pipe diameter greatly affects the pressure oscillations. This dramatically depends on the pump pressure and is mainly caused by the wave and acoustic interactions in the oscillator. Feeding pipe which induces the maximum peak is the one that results in the minimum amplitude, and vice versa. Moreover, feeding pipe of diameter of 25 mm makes the amplitude fluctuate more violently at lower pump pressures. Further investigations related to the interactions of feeding pipe, hydroacoustic waves, and vorticities in shear layer should be performed.
Deng Li,Yong Kang,Xiaolong Ding,Xiao-Chuan Wang,Zhenlong Fang 대한기계학회 2016 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.30 No.11
The destructive power of a continuous waterjet issuing from a nozzle can be greatly enhanced by generating self-resonance in the nozzle assembly to produce a Self-resonating pulsed waterjet (SRPW). To further improve the performance of SRPW, effects of feeding pipe diameter on the pressure characteristics were experimentally investigated by measuring and analyzing the axial pressure oscillation peaks and amplitudes. Four organ-pipe nozzles of different chamber lengths and three feeding pipes of different diameters were employed. Results show that feeding pipe diameter cannot change the feature of SRPW of having an optimum standoff distance, but it slightly changes the oscillating frequency of the jet. It is also found that feeding pipe diameter significantly affects the magnitudes of pressure oscillation peak and amplitude, largely depending on the pump pressure and standoff distance. The enhancement or attenuation of the pressure oscillation peak and amplitude can be differently affected by the same feeding pipe diameter.
Chuan He,Yongqian Wu,Erping Deng,Yawen Zhou,Zhu Zhang,Lijian Ding 한국고분자학회 2024 폴리머 Vol.48 No.1
Dielectric barrier discharge (DBD) is a commonly used plasma surface modification technique, and the power parameters play a crucial role in influencing the modification results. Electrical properties of DBD pulses were studied by varying both power and frequency. Additionally, how these electrical properties influence the chemical and topographical alterations in polyethylene surface modification was investigated. In terms of chemical changes, we found that higher power supply significantly enhances the modification result, whereas alterations in frequency do not exert a substantial impact. Concerning topographical changes, increased power supply yielded similarly effective results, while heightened supply frequency improved the modification result due to the enhanced uniformity of discharge pulses. Regarding hydrophobicity, power supply exerts a more significant and pronounced influence compared to frequency.
Xi-sheng Xie,Fei-yan Li,Heng-chuan Liu,Yao Deng,Zi Li,Jun-ming Fan 대한약학회 2010 Archives of Pharmacal Research Vol.33 No.2
The effects of LSKL, the peptide antagonist of thrombospondin-1 (TSP-1), on renal interstitial fibrosis in rats subjected to unilateral ureteral obstruction (UUO) were investigated. Rats were divided randomly into three groups (n = 20 each): UUO group, sham-operation group and UUO plus LSKL treatment group. Collagen deposition was studied using histopathology and reverse transcription polymerase chain reaction analysis (RT-PCR). TSP-1, transforming growth factor beta 1 (TGF-β1), phosphorylated Smad2 (pSsmad2) and α-smooth muscle actin (α-SMA) in the kidney were measured using immunocytochemistry, western blotting analysis, RT-PCR and enzyme-linked immunosorbent assay. Biochemical analyses in the serum and urine were made. Histopathology showed severe tubular dilatation and atrophy, interstitial inflammation and collagen accumulation after surgery and LSKL significantly inhibited interstitial fibrosis including tubular injury as well as collagen deposition. The protein and mRNA levels of TSP-1 increased notably at different time point and significantly decreased in the presence of LSKL. The expression of TGF-β1 and pSmad2 were upregulated in the obstructed kidney and substantially suppressed by LSKL treatment. Myofibroblast accumulation could be alleviated after administration of LSKL. Biochemical parameters did not show differences among the three groups. As TSP-1 is the major activator of TGF-β1, we demonstrate that LSKL can attenuate renal interstitial fibrosis in vivo by preventing TSP-1-mediated TGF-β1 activation.
Yue-Feng Wang,Xue-Yue Hou,Chuan-Ying Jiang,Tong-Tong Zhai,Rui Miao,Jun-Jie Deng,Zhi-Hong Yao,Rongshu Zhang 한국원예학회 2021 Horticulture, Environment, and Biotechnology Vol.62 No.2
Trichoderma spp. are widely applied, eco-friendly mycofungicides and plant growth promoters. Native Trichoderma strainsare likely to have more productive, stable biocontrol and biofertilizer activities since they are well adapted to the local environment. In this study, we isolated a native Trichoderma strain ‘Th62’ from the rhizosphere soil of wild Chelidonium majusplants in Harbin, China (126.6341°E, 45.7242°N). The isolated Trichoderma strain was identifi ed as a T. harzianum strainvia morphological observation and molecular methods based on the rDNA internal transcribed spacer region and elongationfactor-1α gene sequences. Signifi cant antagonistic activities of Th62 against fi ve soil-borne fungal phytopathogens,Fusarium oxysporum , Sclerotinia sclerotiorum , Alternaria alternata , Cytospora chrysosperma, and Rhizoctonia solani ,were confi rmed by dual-culture assays. Furthermore, the crude fermentation products of Th62 also displayed antifungalactivities against these fi ve pathogens. To evaluate the function of Th62 as a biofertilizer, we subsequently applied Th62on cockscomb ( Celosia cristata L), a plant species with both ornamental and medicinal values, by inoculation with Th62conidia at diff erent concentrations, 1 × 10 10 cfu mL −1 , 1 × 10 11 cfu mL −1 , and 1 × 10 12 cfu mL −1 . The benefi cial eff ects ofTh62 were evaluated by measuring the growth and photosynthetic traits of the inoculated cockscomb plants, and the resultsdemonstrated that Th62 signifi cantly improved the photosynthetic effi ciency, photosynthetic capacity, and the adaptabilityto intense light of the inoculated cockscomb plants compared to the controls. Consistently, Th62 inoculation signifi cantlyimproved the growth and fl ower yield of cockscomb. We presented a positive case of isolating and applying native microbialresources on local plantation practices.
Biphasic effects of TGFβ1 on BMP9-induced osteogenic differentiation of mesenchymal stem cells
( Rui Dong Li ),( Zhong Liang Deng ),( Ning Hu ),( Xi Liang ),( Bo Liu ),( Jin Yong Luo ),( Liang Chen ),( Liang Jun Yin ),( Xiao Ji Luo ),( Wei Shui ),( Tong Chuan He ),( Wei Huang ) 생화학분자생물학회(구 한국생화학분자생물학회) 2012 BMB Reports Vol.45 No.9
We have found that the previously uncharacterized bone morphogenetic protein-9 (BMP9) is one of the most osteogenic factors. However, it is unclear if BMP9 cross-talks with TGFβ1 during osteogenic differentiation. Using the recombinant BMP9 adenovirus, we find that low concentration of rhTGFβ1 synergistically induces alkaline phosphatase activity in BMP9-transduced C3H10T1/2 cells and produces more pronounced matrix mineralization. However, higher concentrations of TGFβ1 inhibit BMP9-induced osteogenic activity. Real-time PCR and Western blotting indicate that BMP9 in combination with low dose of TGFβ1 potentiates the expression of later osteogenic markers osteopontin, osteocalcin and collagen type 1 (COL1a2), while higher concentrations of TGFβ1 decrease the expression of osteopontin and osteocalcin but not COL1a2. Cell cycle analysis reveals that TGFβ1 inhibits C3H10T1/2 proliferation in BMP9-induced osteogenesis and restricts the cells in G0/G1 phase. Our findings strongly suggest that TGFβ1 may exert a biphasic effect on BMP9-induced osteogenic differentiation of mesenchymal stem cells. [BMB Reports 2012; 45(9): 509-514]
The complete mitochondrial genome of a threatened loach (Sinibotia reevesae) and its phylogeny
Yuan‑Chao Zou,Bi‑Wen Xie,Chuan‑Jie Qin,Yong‑Ming Wang,Deng‑Yue Yuan,Rui Li,Zheng‑Yong Wen 한국유전학회 2017 Genes & Genomics Vol.39 No.7
In present study, the complete mitochondrial genome of Sinibotia reevesae was first sequenced using the next-generation sequencing technology and annotated using bioinformatic tools. The circular mitochondrial genome was 16,572 bp in length, and contained 13 proteincoding genes, 2 ribosomal RNA genes, 22 transfer RNA genes, and 1 displacement loop locus. It presents a typical gene organization and order for completely sequenced cypriniformes mitogenomes. The control region could be divided into three parts included the extended termination associated sequence domain, the central conserved domain and the conserved sequence block. Interestingly, two stemloop domains were found in control region and OL region, respectively. Furthermore, phylogenetic analyses using concatenated amino acid and nucleotide sequences of the 13 protein-coding genes with two different methods (Maximum likelihood and Bayesian analysis) both highly supported the close relationship of S. reevesae and Sinibotia superciliaris, which was in line with the previous classifications based on morphological and molecular studies. These data provide useful information for a better understanding of the mitogenomic diversities and evolution in fish as well as novel genetic markers for studying population genetics and species identification.