Experience of Congenital Choledochal Cyst in Adults:Treatment, Surgical Procedures and Clinical Outcome in the Second Affiliated Hospital of Harbin Medical University

De Quan Wu,Qiu Shi Wang,Hong Bo Jia,Hong Shang,Xiang Yu Zhong,Wen Xue Zhou,Zhen Hua Sun,Long Xian Zheng 대한의학회 2004 Journal of Korean medical science Vol.19 No.6

Optimization of Protoplast Preparation and Regeneration of a Medicinal Fungus Antrodia cinnamomea

( Jyun-de Wu ),( Jyh-ching Chou ) 한국균학회 2019 Mycobiology Vol.47 No.4

Antrodia cinnamomea is a unique medicinal fungus in Taiwan. It has been found rich in some pharmacologically active compounds for anti-cancer, hangover, and immune regulation etc. With the in-depth study of these components, it would be interesting and important to establish a molecular system for basic studies of A. cinnamomea. Thus, we would like to set up a foundation for this purpose by studying the A. cinnamomea protoplast preparation and regeneration. Firstly, we studied the optimization method of protoplast preparation of A. cinnamomea, and found various factors that may affect the yield during protoplast preparation, such as mycelial ages, pH values, and osmotic stabilizers. Secondly, in the regeneration of protoplasts, we explored the effects of various conditions on the regeneration of protoplasts, including different media and osmotic pressure. In addition, we found that citrate buffer with pH value around 3 dramatically increased the regeneration of protoplasts of A. cinnamomea, and provided a set of regeneration methodology for A. cinnamomea.

Association of Polymorphisms in Stress-Related TNFα and NPY Genes with the Metabolic Syndrome in Han and Hui Ethnic Groups

Bu, De-Yun,Ji, Wen-Wu,Bai, Dan,Zhou, Jian,Li, Hai-Xia,Yang, Hui-Fang Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.14

Background: Metabolic syndrome (MS) is a cluster of complicated disorders caused by the interactive influencing factors of heredity and environment, which predisposes to many cnacers. Results from epidemic research indicate that stress is tightly related to the pathogenesis of MS and neoplasia. This paper aims to investigate the association between psychological stress and MS with respect to the tumor necrosis factor alpha (TNF${\alpha}$) and neuropeptide Y (NPY) genes in the Han and Hui ethnic groups. Methods: All subjects for this case-control study matched strict enrollment criteria (nationality, gender and age) and lived in the city of Wu Zhong of Ningxia Province in China. The enrolled group contained 102 matched pairs of Hui ethnic individuals and 98 matched pairs of Han ethnic individuals. Enrolled subjects completed the general Symptom Checklist-90 (SCL-90). The TNF${\alpha}$-308G/A variant and NPYrs16147 polymorphism were detected in case (81 males, 119 females) and control (81 males, 119 females) groups by polymerase chain reaction (PCR) amplification. Results: Nine factors of the SCL-90 were found to be statistically different (p<0.05) between case and control groups. The homozygous mutant genotype (AA) and the mutant allele (A) of the TNF${\alpha}$-308G/A gene were less frequently observed in the control population compared to the case group. The odds ratio (95% confidence interval) in "Allele" for MS was 2.28 (1.47-3.53), p=0.0001, while "OR" was 1.11 (0.83-1.47), p=0.15, for the NPYrs16147 gene polymorphism. Conclusions: Psychological stress has been positively associated with MS. A previous study from our group suggested there were differences in the level of psychological stress between Hui and Han ethnic groups. Furthermore, we found that the stress-related TNF${\alpha}$ gene was associated with MS for both Han and Hui ethnic groups. In contrast, NPY may be a possible contributor to MS and associated cancer for the Han ethnic group.

AutoCAD 2D 기반 공동주택 이격거리 자동체크 시스템

오성덕(Wu, Cheng-De) 대한건축학회 2010 대한건축학회 학술발표대회 논문집 - 계획계/구조계 Vol.30 No.1(계획계)

2002 부산아시아경기대회 기념 국제학술회의 음악 , 아시아 그리고 다문화 당대 중국 고대음악 미학사상연구 현황

오금덕(Jin De Wu) 민족음악학회 2002 음악과 민족 Vol.24 No.-

There is no doubt that the Han in ancient society shared Chinese culture with other neighbouring people. In this Chinese influence on Korean culture, it would certainly be valuable for Korean scholars to understand the current research situation on aesthetic ideas on Chinese traditional ancient music engaged in native Chinese scholars. This paper aims to address its ongoing research trends in China, thus helping Korean counterparts, who are involved in this field, enable to apply its actual situation to their studies. This paper intends largely to introduce the current methods of the studies on aesthetic ideas on music in Chinese ancient time which is particularly referred to the book A History of Aesthetics on Chinese Music(中國音樂美學史) written by Che Juin Du (蔡仲德), who is a professor at Jung`ang Conservatory/Academy of Music in Beijing. The content is as follows: 1. It deals with a chronical division and research object of the history of aesthetics on music practised in Chinese ancient time; 2. It includes aesthetic ideas on music emerged from Chinese traditional Buddhism and Taoism; 3. It deals also with main thesis arrived from aesthetic ideas on music in Chinese ancient time, and this section can be subdivided into six ideas: (i) relationship between feeling (情) and virtue (德 or 禮); (ii) relationship between voice (聲) and the way (導); (iii) relationship between greed (慾) and the way; (iv) relationship between sorrow (悲) and beauty (美); (v) relationship between music (樂) and politics (政); (vi) relationship between the past (古) and the present (今), that is, refinement (雅) and politeness (鄭). 4. It is to reveal some characteristic features of aesthetic ideas on music practised in Chinese ancient time, for instance, (i) idea of Yeak (예악禮樂, lit. means etiquette and music) ; (ii) criteria of aestheticism of Jungwha 중화中和 (lit. means counteraction), Damwha 담화淡和(lit. means clearness) and Pyeongwhayeomdam 평화념담平和恬淡 (lit. means harmony and unselfness); (iii) Cheon-In-Hap-ll (천인합일天人合一, lit. means heaven and human being become oneness) and the theory of Um-Yang-O-Haeng (음양오행陰陽五行, lit. means the dual principle of the negative (or female) and positive (male)) and The Five Elements which constitute metal, wood, water, ire and earth); (iv) There are many statements about philosophical, ethical and political relationship between sound (음音) and music theory (악樂) appeared in among the Chinese antiquity or old literature, but there is lack of the statement about regulations, peculiarity, pleasure within music itself; (v) Its thought attained a full-fledged stage in very earlier time, but its developments became inactive in later time. 5. Lastly this paper is also concerned with some research trends on aesthetic thought on music paractised in Chinese ancient time.

Differentially Rice Protein Expression Between Rice Bran and Endosperm

( Ting-yu Chen ),( De-min Wu ),( Ji-jyun Lai ),( Chang-yue Li ),( Hui-fen Liao ) 한국농업기계학회 2018 한국농업기계학회 학술발표논문집 Vol.23 No.1

Rice (Oryza sativa L.), the major staple food for more than 60% of the world’s population, offer nutritional and health-enhancing properties. Therefore, breeding of new rice species has been fueled by the rising interest in Asian, Latin cuisines, and many countries. In Asia, rice and rice-based ingredients also appeal to both consumers and processors due to their unique combination of taste, nutrition, texture, and biological properties. Proteins and starch in rice are the two major components in rice seed, with approximately 8 and 80%, respectively. Especially in traditional Asian diet, rice seed contributes to about 28-54% of the protein source. The major rice proteins, including structural, metabolic, protective, and storage proteins, serve as sources of nitrogen, sulfur, and carbon for several important physiological functions. In our previous study also demonstrated that rice protein prolamin activated human mononuclear cells to produce cytokines and enhance anti-leukemic immunity. The present study aimed to compare the differentially expression of rice proteins with bran and endosperm by 2-dimentional electrophoresis (2-DE) and mass spectrometric assay. Several protein spots in 2-DE gel with different expression were isolated and identified. The results showed that the major proteins were metabolic, transporter, storage, antioxidant, disease resistant, and development-related proteins. Further investigation to clarify the different manifestations and functions of these proteins might contribute to development of new rice varieties and breeding with unique features.

miR-27a as an Oncogenic microRNA of Hepatitis B Virus-related Hepatocellular Carcinoma

Wu, Xin-Jun,Li, Yan,Liu, Dong,Zhao, Lun-De,Bai, Bin,Xue, Ming-Hui Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.2

microRNAs (miRNAs) are small non-coding RNAs that regulate gene expression through post-transcriptional interactions with mRNA. miRNAs have recently emerged as key regulators of various cancers. Although miR-27a has been implicated in several other cancers, its role in hepatitis B virus-related hepatocellular carcinoma (HCC) is unknown. In this study, we showed miR-27a to be frequently up-regulated in HCC tissues and HCC cell lines (HepG2 and Huh7). Overexpression of miR-27a enhanced cell proliferation, promoted migration and invasion, and activated cell cycling in HepG2 and Huh7 cells. In summary, our results suggest that up-regulation of miR-27a may play an oncogenic role in the development of HCC and might thus be a new therapeutic target in HCC patients.

Biodegradation of Di-n-Butyl Phthalate by Rhodococcus sp. JDC-11 and Molecular Detection of 3,4-Phthalate Dioxygenase Gene

( De Cai Jin ),( Ren Xing Liang ),( Qin Yun Dai ),( Rui Yong Zhang ),( Xue Liu Wu ),( Wei Liang Chao ) 한국미생물 · 생명공학회 2010 Journal of microbiology and biotechnology Vol.20 No.10

Rhodococcus sp. JDC-11, capable of utilizing di-n-butyl phthalate (DBP) as the sole source of carbon and energy, was isolated from sewage sludge and confirmed mainly based on 16S rRNA gene sequence analysis. The optimum pH, temperature, and agitation rate for DBP degradation by Rhodococcus sp. JDC-11 were 8.0, 30oC, and 175 rpm, respectively. In addition, low concentrations of glucose were found to inhibit the degradation of DBP, whereas high concentrations of glucose increased its degradation. Meanwhile, a substrate utilization test showed that JDC- 11 was also able to utilize other phthalates. The major metabolites of DBP degradation were identified as monobutyl phthalate and phthalic acid by gas chromatographymass spectrometry, allowing speculation on the tentative metabolic pathway of DBP degradation by Rhodococcus sp. JDC-11. Using a set of new degenerate primers, a partial sequence of the 3,4-phthalate dioxygenase gene was obtained from JDC-11. Moreover, a sequence analysis revealed that the phthalate dioxygenase gene of JDC-11 was highly homologous to the large subunit of the phthalate dioxygenase from Rhodococcus coprophilus strain G9.

Promoted Growth of Maize by the Phosphate Solubilizing Bacteria Isolated from North-east China

Wu, Hai-Yan,Wang, Li-Chun,Gao, Xing-Ai,Jin, Rong-De,Fan, Zuo-Wei,Kim, Kil-Yong,Zhao, Lan-Po Korean Society of Soil Science and Fertilizer 2011 한국토양비료학회지 Vol.44 No.1

A strain of phosphate solubilizing bacterium was isolated from rhizosphere and identified as Burkholderia sp. by 16S-rRNA gene sequence analyses. The bacterium was found to release gluconic acid and the solubilization of hydroxyapatite in the liquid medium by a significant drop in pH to 3.7 from an initial pH 7.0. The soluble-P concentration continuously increased during the incubation periods and the total amount of soluble P released in culture filtrate was detected at 990 mg $L^{-1}$ after 10 days of inoculation. Most promoted maize growth was found in the standard NPK (240-120-120 kg $ha^{-1}$) soil inoculation with Burkholderia sp. (Twenty milliliters/plant, 106 CFU) and also in the absence of Burkholderia sp. inoculation, the soil amended with only 2/3 levels of P gave significant higher plant yield compared to 1/3 levels of P or without P supplementation.

Single-particle characterization of aerosols collected at a remote site in the Amazonian rainforest and an urban site in Manaus, Brazil

Wu, Li,Li, Xue,Kim, HyeKyeong,Geng, Hong,Godoi, Ricardo H. M.,Barbosa, Cybelli G. G.,Godoi, Ana F. L.,Yamamoto, Carlos I.,de Souza, Rodrigo A. F.,Pö,hlker, Christopher,Andreae, Meinrat O.,Ro, Chul Copernicus GmbH 2019 Atmospheric chemistry and physics Vol.19 No.2

<P><p><strong>Abstract.</strong> <span id='page1222'/>In this study, aerosol samples collected at a remote site in the Amazonian rainforest and an urban site in Manaus, Brazil, were investigated on a single-particle basis using a quantitative energy-dispersive electron probe X-ray microanalysis (ED-EPMA). A total of 23 aerosol samples were collected in four size ranges (0.25-0.5, 0.5-1.0, 1.0-2.0, and 2.0-4.0<span class='thinspace'></span><span class='inline-formula'>µ</span>m) during the wet season in 2012 at two Amazon basin sites: 10 samples in Manaus, an urban area; and 13 samples at an 80<span class='thinspace'></span>m high tower, located at the Amazon Tall Tower Observatory (ATTO) site in the middle of the rainforest, 150<span class='thinspace'></span>km northeast of Manaus. The aerosol particles were classified into nine particle types based on the morphology on the secondary electron images (SEIs) together with the elemental concentrations of 3162 individual particles: (i) secondary organic aerosols (SOA); (ii) ammonium sulfate (AS); (iii) SOA and AS mixtures; (iv) aged mineral dust; (v) reacted sea salts; (vi) primary biological aerosol (PBA); (vii) carbon-rich or elemental carbon (EC) particles, such as soot, tarball, and char; (viii) fly ash; and (ix) heavy metal (HM, such as Fe, Zn, Ni, and Ti)-containing particles. In submicron aerosols collected at the ATTO site, SOA and AS mixture particles were predominant (50<span class='thinspace'></span>%-94<span class='thinspace'></span>% in relative abundance) with SOA and ammonium sulfate comprising 73<span class='thinspace'></span>%-100<span class='thinspace'></span>%. In supermicron aerosols at the ATTO site, aged mineral dust and sea salts (37<span class='thinspace'></span>%-70<span class='thinspace'></span>%) as well as SOA and ammonium sulfate (28<span class='thinspace'></span>%-58<span class='thinspace'></span>%) were abundant. PBAs were observed abundantly in the PM<span class='inline-formula'>₂₋₄</span> fraction (46<span class='thinspace'></span>%), and EC and fly ash particles were absent in all size fractions. The analysis of a bulk PM<span class='inline-formula'>_0.25−0.5</span> aerosol sample from the ATTO site using Raman microspectrometry and attenuated total reflection Fourier-transform infrared spectroscopy (ATR-FTIR) showed that ammonium sulfate, organics, and minerals are the major chemical species, which is consistent with the ED-EPMA results. In the submicron aerosols collected in Manaus, either SOA and ammonium sulfate (17<span class='thinspace'></span>%-80<span class='thinspace'></span>%) or EC particles (6<span class='thinspace'></span>%-78<span class='thinspace'></span>%) were dominant depending on the samples. In contrast, aged mineral dust, reacted sea salt, PBA, SOA, ammonium sulfate, and EC particles comprised most of the supermicron aerosols collected in Manaus. The SOA, ammonium sulfate, and PBAs were mostly of a biogenic origin from the rainforest, whereas the EC and HM-containing particles were of an anthropogenic origin. Based on the different contents of SOA, ammonium sulfate, and EC particles among the samples collected in Manaus, a considerable influence of the rainforest over the city was observed. Aged mineral dust and reacted sea-salt particles, including mineral dust mixed with sea salts probably during long-range transatlantic transport, were abundant in the supermicron fractions at both sites. Among the aged mineral dust and reacted sea-salt particles, sulfate-containing ones outnumbered those containing nitrates and sulfate<span class='thinspace'></span><span class='inline-formula'>+</span><span class='thinspace'></span>nitrate in the ATTO samples. In contrast, particles containing sulfate<span class='thinspace'></span><span class='inline-formula'>+</span><span class='thinspace'></span>nitrate were comparable in number to particles containing sulfate only in the Manaus samples, indicating