Improvement of 2,2′-Azobis(2-Methylpropionamidine) Dihydrochloride-Induced Hepatic Redox Imbalance in Swiss Mice and HepG2 Cells by Rutin

Paula Alexandre de Freitas,Keciany Alves de Oliveira,Laryssa Alves Magalha˜es,Regina de Jesus das Neves,Carla Soraya Costa Maia,Leonardo Reis Silveira,Tanes Tamamura de Lima,Renata Prado Vasconcelos,L 한국식품영양과학회 2022 Journal of medicinal food Vol.25 No.6

Redox imbalance can lead to irreversible damages to biological functions. In this context, rutin stands out for its antioxidant potential. The objective of this study was to evaluate the acute and chronic effect of rutin on the hepatic redox imbalance. The study was performed according to three different protocols. First, healthy male Swiss mice were divided into two groups: control and rutin, the second of which received chronic oral supplementation of rutin (10 mg/kg). The second involved evaluation of the generation of reactive oxygen species (ROS) by HepG2 cells, incubated or not with rutin (20 and 40 μg/mL) for 3 h. The final protocol involved assessment of the acute effect of rutin (10 mg/kg) in mice with oxidative stress induced by 2,2′-azobis(2-methylpropionamidine) dihydrochloride (ABAP). After the in vivo treatments, the livers were collected to analyze the oxidative damage by thiol, and the antioxidant defense by catalase, superoxide dismutase, and glutathione peroxidase. In the HepG2 cells, the following probes were employed to assess the ROS production: dichlorofluorescein, MitoSOX, dihydroethidium, and Amplex Red. Rutin administered chronically improved the antioxidant defense in healthy animals, and when administered acutely both inhibited the increased production of ROS in HepG2 cells and improved the redox imbalance parameters in mice with induced oxidative stress. This study suggests rutin as a protective agent for restoration of hepatic redox homeostasis in redox injury induced by ABAP in Swiss mice and HelpG2 cells.

Prevalent Signs and Symptoms in Patients with Skin Cancer and Nursing Diagnoses

Lisboa, Isabel Neves Duarte,de Azevedo Macena, Monica Suela,da Conceicao Dias Fernandes, Maria Isabel,de Almeida Medeiros, Ana Beatriz,de Lima, Cyndi Fernandes,de Carvalho Lira, Ana Luisa Brandao Asian Pacific Journal of Cancer Prevention 2016 Asian Pacific journal of cancer prevention Vol.17 No.7

Background: Skin cancer has a remarkable importance given the high incidence in the population. In Brazil, it is estimated that there were 98,420 new cases of non-melanoma skin cancer among men and 83,710 new cases among women in 2014. Objectives: To verify signs and symptoms present in patients with skin neoplasms according to the literature and relate them to the nursing diagnoses of NANDA International. Materials and Methods: Integrative literature review carried out from March to May 2015 in the databases: Cumulative Index to Nursing and Allied Health Literature, SCOPUS, National Library of Medicine and Nattional Institutes of Health, Latin American and Caribbean Sciences of Health and Web of Science. The descriptors used were: 'Signs and Symptoms' and 'Skin Neoplasms'. Sixteen articles were identified as the final sample. After review, the signs and symptoms of skin cancer identified in the literature were related to the defining characteristics present in NANDA International, with the aim to trace possible nursing diagnoses. Results: The most prevalent signs and symptoms were: asymmetric and well circumscribed nodules with irregular borders; speckles with modified color aspect; ulcerations; blisters; pain; itching; and bleeding. The principal nursing diagnoses outlined were: risk for impaired skin integrity; impaired skin integrity; acute pain; risk of shock; and impaired comfort. Conclusions: The identification of signs and symptoms present in patients with skin cancer and the relationships of these with the nursing diagnoses of NANDA International provide a basis for qualified and systematized nursing care to this clientele.

Porosity and pore size distribution in high-viscosity and conventional glass ionomer cements: a micro-computed tomography study

Neves Aline Borburema,Lopes Laísa Inara Gracindo,Bergstrom Tamiris Gomes,Silva Aline Saddock Sá da,Lopes Ricardo Tadeu,Neves Aline de Almeida 대한치과보존학회 2021 Restorative Dentistry & Endodontics Vol.46 No.4

Objectives This study aimed to compare and evaluate the porosity and pore size distribution of high-viscosity glass ionomer cements (HVGICs) and conventional glass ionomer cements (GICs) using micro-computed tomography (micro-CT). Materials and Methods Forty cylindrical specimens (n = 10) were produced in standardized molds using HVGICs and conventional GICs (Ketac Molar Easymix, Vitro Molar, MaxxionR, and Riva Self-Cure). The specimens were prepared according to ISO 9917-1 standards, scanned in a high-energy micro-CT device, and reconstructed using specific parameters. After reconstruction, segmentation procedures, and image analysis, total porosity and pore size distribution were obtained for specimens in each group. After checking the normality of the data distribution, the Kruskal-Wallis test followed by the Student-Newman-Keuls test was used to detect differences in porosity among the experimental groups with a 5% significance level. Results Ketac Molar Easymix showed statistically significantly lower total porosity (0.15%) than MaxxionR (0.62%), Riva (0.42%), and Vitro Molar (0.57%). The pore size in all experimental cements was within the small-size range (< 0.01 mm3), but Vitro Molar showed statistically significantly more pores/defects with a larger size (> 0.01 mm3). Conclusions Major differences in porosity and pore size were identified among the evaluated GICs. Among these, the Ketac Molar Easymix HVGIC showed the lowest porosity and void size.

Can the body composition of crossbred dairy cattle be predicted by equations for beef cattle?

Neves, Maria Luciana Menezes Wanderley,de Souza, Evaristo Jorge Oliveira,Veras, Robson Magno Liberal,de Campos Valadares Filho, Sebastiao,Marcondes, Marcos Inacio,da Silva, Gabriel Santana,Barreto, Li Asian Australasian Association of Animal Productio 2018 Animal Bioscience Vol.31 No.10

Objective: The aim of the study was to evaluate the efficiency of the Hankins and Howe (HH46), Valadares Filho (V06), and Marcondes (M12) equations for predicting the physical and chemical composition of dairy crossbred bulls carcasses, as well as the chemical composition of their empty bodies. Methods: This study was conducted using 30 dairy crossbred bulls. One group of five animals was slaughtered at the beginning of the experiment, and the remaining were slaughtered 112 days later. Animals were distributed in a completely randomized design into treatments consisting different levels of concentrate (0%, 17%, 34%, 51%, and 68%). The physical and chemical compositions of the cattle were obtained from the right half of the carcass and using samples taken between the 9th and 11th ribs of the left half of the carcass. The estimated and experimentally determined values were compared using the correlation and concordance coefficient, as well as the mean square error of prediction (MSEP) and its components. Results: The HH46 equations were better at estimating the amount of muscle plus fat in the carcass. The amount of bone in the carcasses could not be well estimated by the HH46 and M12 models. The M12, HH46, and V06 equations were worst at estimating the amounts of protein, ether extract, and water in the carcass, respectively. In the empty body, the amounts of protein and water were well estimated by the HH46 equations. Protein, ether extract, and water were accurately estimated by the V06 equations, and ether extract by the M12 equations. Conclusion: The physical and chemical composition of dairy crossbred bull carcasses, as well as the chemical composition of their empty bodies, can be predicted using the equations tested here. The amount of bone in these carcasses could not be accurately predicted.

Influence of Gamma Radiation Treatment on the Mechanical Properties of Sisal Fibers to Use into Composite Materials

Bruno Dorneles de Castro,Kláudia Maria Machado Neves Silva,Rômulo Maziero,Paulo Eustáquio de Faria,Priscila Pereira Silva-Caldeira,Juan Carlos Campos Rubio 한국섬유공학회 2020 Fibers and polymers Vol.21 No.8

The use of treatments in natural fibers is largely used, because it can improve the interfacial adhesion of polymercomposites. However, the choice of the most appropriate treatment should take into account environmental factors, due to theneed of new materials to meet requirements related to the concept of sustainability. This article investigated the influence ofthe mercerization, acetylation and gamma radiation treatments on the mechanical properties of sisal fibers, to use incomposite materials. Experimental conditions were defined with different concentrations of reagents and radiation doses inorder to understand the impact of these reactions on the tensile properties of sisal fibers. Thermal gravimetric analysis (TGA)and Fourier Transform Infrared (FTIR) spectroscopy were used to investigate the fiber degradation process and the chemicalmodifications after the treatments. The use of mercerization (0.1 M and 1.0 M NaOH concentrations) combined withacetylation increased the hydrophobic character of the sisal fiber, which may improve its interaction with polymeric matrices. In addition, mercerized sisal fiber at 0.5 M NaOH concentration, exposed to 25 kGy gamma radiation dose, showed physicaland chemical structural changes that positively influence the fiber-matrix adhesion in polymer composite materials. Alltreatments made can reduce the natural tensile properties of the sisal fibers, because they can remove fundamentalcomponents to the structure and the stiffening of these fibers. Considering changes in the mechanical behavior, the use ofgamma radiation presented similar results of the traditional chemical treatments, being less aggressive to the fibers and notcausing the same negative impact on the environment.

Negative neuromuscular and functional repercussion of forced swimming after axonotmesis

Júlia Araújo de Moura,Jaqueline de Morais,Samara Maria Neves Barbosa,Marcílio Coelho Ferreira,Ivo Vieira de Sousa Neto,Hércules Ribeiro Leite,Murilo Xavier Oliveira,Thaís Peixoto Gaiad,Ana Paula Santo 한국운동재활학회 2022 JER Vol.18 No.3

Inulinase from Rhodotorula mucilaginosa: immobilization and application in the production of fructooligosaccharides

Geise Camila de Araujo Ribeiro,Pedro Fernandes,Dayse Alessandra Almeida Silva,Hugo Neves Brandao,Sandra Aparecida de Assis 한국식품과학회 2021 Food Science and Biotechnology Vol.30 No.7

The crude extract containing inulinase fromRhodotorula mucilaginosa was obtained by submergedfermentation. Inulinase was immobilized on chicken eggshellby physical adsorption and covalent crosslinking,using glutaraldehyde as a crosslinking reagent, and Celiteby adsorption. Fructooligosaccharides production wasperformed using immobilized inulinase (5%, w/v) andinulin substrate solution under experimental conditionsevaluated through Doehlert experimental design. The productionof inulinase was optimized for concentrations ofD-glucose and yeast extract at 12.5 and 0.5 g/L, respectively,resulting in an optimal activity of 0.62 U. Theoptimal pH and temperature for enzyme activity were 8.0and 75 C, respectively, leading to an optimal activity of3.54 U. The highest immobilization efficiency (46.27%)was obtained upon immobilization on Celite. Immobilizationby adsorption to eggshell allowed for specific activityof 4.15 U/g, and adsorption to Celite resulted in specificactivity of 3.70 U/g. The highest titer in fructooligosaccharideswas obtained with an initial inulin concentrationof 250 g/L (25%, w/v), and a reaction time of 16 h. Hence,immobilized inulinase proved to be a promising catalyst forfructooligosaccharides production since the formulation isperformed through a simple, low-cost, and large-scaleapplicable methodology.

Carcass and meat traits of bubaline finished on sugarcane-based diets supplemented with spineless cactus as a replacement for wheat bran

Borges Christiano Raphael de Albuquerque,Carvalho Francisco Fernando Ramos de,Neves Maria Luciana Menezes Wanderley,Pereira Neto José Diógenes,Vieira Guilherme Heliodoro Pedroso,Pessoa Ricardo Alexand 아세아·태평양축산학회 2022 Animal Bioscience Vol.35 No.1

Objective: An experiment was conducted to evaluate the effects of increasing levels of spineless cactus (0%, 33%, 66%, and 100%) used as a substitute for wheat bran in buffalo diets on quantitative and qualitative traits of the meat and carcass. Methods: Twenty Murrah buffaloes at 18 months of age, with a mean initial weight of 292.9±57.3 kg, were randomly allocated to four treatments with five replicates. The animals were slaughtered after 90 days in the feedlot. The effects of spineless cactus as a replacement for wheat bran in the diet of the buffaloes on the carcass and meat traits, slaughter weight, carcass yield and carcass measurements were studied. Results: Increased spineless cactus levels led to linear reduction in average daily gain, slaughter weight, hot and cold carcass weight, compactness index and in the amount of muscle in the carcass, and there is no difference between the control treatment and the 33% replacing level for these parameters. The quality of the meat was not influenced by the treatments. Conclusion: Spineless cactus can replace wheat bran by up to 33% in sugarcane-based diets for buffaloes, without influencing quantitative and qualitative traits of the meat and carcass. Objective: An experiment was conducted to evaluate the effects of increasing levels of spineless cactus (0%, 33%, 66%, and 100%) used as a substitute for wheat bran in buffalo diets on quantitative and qualitative traits of the meat and carcass.Methods: Twenty Murrah buffaloes at 18 months of age, with a mean initial weight of 292.9±57.3 kg, were randomly allocated to four treatments with five replicates. The animals were slaughtered after 90 days in the feedlot. The effects of spineless cactus as a replacement for wheat bran in the diet of the buffaloes on the carcass and meat traits, slaughter weight, carcass yield and carcass measurements were studied.Results: Increased spineless cactus levels led to linear reduction in average daily gain, slaughter weight, hot and cold carcass weight, compactness index and in the amount of muscle in the carcass, and there is no difference between the control treatment and the 33% replacing level for these parameters. The quality of the meat was not influenced by the treatments.Conclusion: Spineless cactus can replace wheat bran by up to 33% in sugarcane-based diets for buffaloes, without influencing quantitative and qualitative traits of the meat and carcass.

Parallel Deblocking Filtering in MPEG-4 AVC/H.264 on Massively Parallel Architectures

Pieters, Bart,Hollemeersch, Charles-Frederik J,De Cock, Jan,Lambert, Peter,De Neve, Wesley,Van de Walle, Rik IEEE 2011 IEEE transactions on circuits and systems for vide Vol.21 No.1

<P>The deblocking filter in the MPEG-4 AVC/H.264 standard is computationally complex because of its high content adaptivity, resulting in a significant number of data dependencies. These data dependencies interfere with parallel filtering of multiple macroblocks (MBs) on massively parallel architectures. In this letter, we introduce a novel MB partitioning scheme for concurrent deblocking in the MPEG-4 AVC/H.264 standard, based on our idea of deblocking filter independency, a corrected version of the limited error propagation effect proposed in the letter. Our proposed scheme enables concurrent MB deblocking of luma samples with limited synchronization effort, independently of slice configuration, and is compliant with the MPEG-4 H.264/AVC standard. We implemented the method on the massively parallel architecture of the graphics processing unit (GPU). Experimental results show that our GPU implementation achieves faster-than real-time deblocking at 1309 frames per second for 1080p video pictures. Both software-based deblocking filters and state-of-the-art GPU-enabled algorithms are outperformed in terms of speed by factors up to 10.2 and 19.5, respectively, for 1080p video pictures.</P>

Push-out bond strength of a self-adhesive resin cement used as endodontic sealer

Gurgel-Filho, Eduardo Diogo,Lima, Felipe Coelho,Saboia, Vicente De Paula Aragao,Coutinho-Filho, Tauby De Souza,Neves, Aline De Almeida,da Silva, Emmanuel Joao Nogueira Leal The Korean Academy of Conservative Dentistry 2014 Restorative Dentistry & Endodontics Vol.39 No.4

Objectives: The aim of the present study was to investigate the bond strength of RelyX Unicem (3M) to root canal dentin when used as an endodontic sealer. Materials and Methods: Samples of 24 single-rooted teeth were prepared with Gates Glidden drills and K3 files. After that, the roots were randomly assigned to three experimental groups (n = 8) according to the filling material, (1) AH Plus (Dentsply De Trey GmbH)/Gutta-Percha cone; (2) Epiphany SE (Pentron)/Resilon cone; (3) RelyX Unicem/Gutta-Percha cone. All roots were filled using a single cone technique associated to vertical condensation. After the filling procedures, each tooth was prepared for a push-out bond strenght test by cutting 1 mm-thick root slices. Loading was performed on a universal testing machine at a speed of 0.5 mm/min. One-way analysis of variance and Tukey test for multiple comparisons were used to compare the results among the experimental groups. Results: Epiphany SE/Resilon showed significantly lower push-out bond strength than both AH Plus/Gutta-Percha and RelyX Unicem/Gutta-Percha (p < 0.05). There was no significant difference in bond strength between AH Plus/Gutta-Percha and RelyX Unicem/Gutta-Percha (p > 0.05). Conclusions: Under the present in vitro conditions, bond strength to root dentin promoted by RelyX Unicem was similar to AH Plus. Epiphany SE/Resilon resulted in lower bond strength values when compared to both materials.