A Newly Identified Glutaminase-Free L-Asparaginase (L-ASPG86) from the Marine Bacterium Mesoflavibacter zeaxanthinifaciens

( Su Jin Lee ),( Youngdeuk Lee ),( Gun Hoo Park ),( Navaneethaiyer Umasuthan ),( Soo Jin Heo ),( Mahanama De Zoysa ),( Won Kyo Jung ),( Dae Won Lee ),( Hanjun Kim ),( Do Hyung Kang ),( Chulhong Oh ) 한국미생물 · 생명공학회 2016 Journal of microbiology and biotechnology Vol.26 No.6

L-Asparaginase (E.C. 3.5.1.1) is an enzyme involved in asparagine hydrolysis and has the potential to effect leukemic cells and various other cancer cells. We identified the Lasparaginase gene (L-ASPG86) in the genus Mesoflavibacter, which consists of a 1,035 bp open reading frame encoding 344 amino acids. Following phylogenetic analysis, the deduced amino acid sequence of L-ASPG86 (L-ASPG86) was grouped as a type I asparaginase with respective homologs in Escherichia coli and Yersinia pseudotuberculosis. The L-ASPG86 gene was cloned into the pET-16b vector to express the respective protein in E. coli BL21 (DE3) cells. Recombinant L-asparaginase (r-L-ASPG86) showed optimum conditions at 37-40oC, pH 9. Moreover, r-L-ASPG86 did not exhibit glutaminase activity. In the metal ions test, its enzymatic activity was highly improved upon addition of 5 mM manganese (3.97-fold) and magnesium (3.35-fold) compared with the untreated control. The specific activity of r-LASPG86 was 687.1 units/mg under optimum conditions (37°C, pH 9, and 5 mM MnSO4).

經營規模가 農業所得에 미치는 影響에 關한 小考

徐大振 진주산업대학교 1977 論文集 Vol.15 No.-

Data used in this study were obtained from surueys of business operations of 300 Karean irop farms in the calendar year 1975, in order to determine and measure the faitors affecting farm income. Farms included in this study were from a probobility sample and were selected on the basis of farm size and Jinju Agri and Ferestry Junior Technical college. Based upon the analysis in this study the fallowing conclusions may be made : 1. The size of farm business itself is in an important factor in determining family labor income. And yet it, is not always nececsary to increase size of farm business for a high income mininwm size of farm business for on efficient operation appears to be 14∼16 tanbo. 2. Production rates had be greatest effect on family labor income. To be efficcint it is advisable to have 3.5 to 4.0 suk of rice yield per tanbo. For high rates of production, it is also advisable to adopt intensive farming practices by using more inputs per unit of land and have a high degree of land utilization through multi-cropping. 3. In general, no critical problem in the use of labor is indicated. It should be remembered, however, that labor is the cheapest substitute for capital items. It is desirable to have 25 to 30 hop of rice yield per hour of labor and 2,500 to 3,500 won of receipts per 10 hours of labor.

Characterization of rDNAs and tandem repeats in the heterochromatin of Brassica rapa.

Lim, Ki-Byung,de Jong, Hans,Yang, Tae-Jin,Park, Jee-Young,Kwon, Soo-Jin,Kim, Jung Sun,Lim, Myung-Ho,Kim, Jin A,Jin, Mina,Jin, Yong-Moon,Kim, Seog Hyung,Lim, Yong Pyo,Bang, Jae-Wook,Kim, Ho-Il,Park, Be Korean Society for Molecular Biology 2005 Molecules and cells Vol.19 No.3

<P>We describe the morphology and molecular organization of heterochromatin domains in the interphase nuclei, and mitotic and meiotic chromosomes, of Brassica rapa, using DAPI staining and fluorescence in situ hybridization (FISH) of rDNA and pericentromere tandem repeats. We have developed a simple method to distinguish the centromeric regions of mitotic metaphase chromosomes by prolonged irradiation with UV light at the DAPI excitation wavelength. Application of this bleached DAPI band (BDB) karyotyping method to the 45S and 5S rDNAs and 176 bp centromere satellite repeats distinguished the 10 B. rapa chromosomes. We further characterized the centromeric repeat sequences in BAC end sequences. These fell into two classes, CentBr1 and CentBr2, occupying the centromeres of eight and two chromosomes, respectively. The centromere satellites encompassed about 30% of the total chromosomes, particularly in the core centromere blocks of all the chromosomes. Interestingly, centromere length was inversely correlated with chromosome length. The morphology and molecular organization of heterochromatin domains in interphase nuclei, and in mitotic and meiotic chromosomes, were further characterized by DAPI staining and FISH of rDNA and CentBr. The DAPI fluorescence of interphase nuclei revealed ten to twenty conspicuous chromocenters, each composed of the heterochromatin of up to four chromosomes and/or nucleolar organizing regions.</P>

Characterization of rDNAs and Tandem Repeats in the Heterochromatin of Brassica rapa

Beom-Seok Park,임기병,Hans de Jong,Tae-Jin Yang,Jee-Young Park,Soo-Jin Kwon,Jung Sun Kim,Myung-Ho Lim,Jin A Kim,Mina Jin,Yong-Moon Jin,Seog Hyung Kim,임용표,방재욱,Ho-Il Kim 한국분자세포생물학회 2005 Molecules and cells Vol.19 No.3

We describe the morphology and molecular organization of heterochromatin domains in the interphase nuclei, and mitotic and eiotic chromosomes, of Brassica rapa, using DAPI staining and fluorescence in situhybridization (FISH) of rDNA and ericentromere tandem repeats. We have developed a simple method to distinguish the centromeric regions of mitotic metaphase chromosomes by prolonged irradiation with UV light at the DAPI excitation wavelength. Application of this bleached DAPI band (BDB) karyotyping method to the 45S and 5S rDNAs and 176 bp centromere satellite repeats distinguished the 10 B. rapa chromosomes. We further characterized the centromeric repeat sequences in BAC end sequences. These fell into two classes, CentBr1 and CentBr2, occupying the centromeres of eight and two chromosomes, respectively. The centromere satellites encompassed about 30% of the total chromosomes, particularly in the core centromere blocks of all the chromosomes. Interestingly, centromere length was inversely correlated with chromosome length. The morphology and molecular organization of heterochromatin domains in interphase nuclei, and in mitotic and meiotic chromosomes, were further characterized by DAPI staining and FISH of rDNA and CentBr. The DAPI fluorescence of interphase nuclei revealed ten to twenty conspicuous chromocenters, each composed of the heterochromatin of up to four chromosomes and/or nucleolar organizing regions.

The Trajectory of University Science Parks (USPs) in China

De-Jin Su,Bei Wu,Dong-Won Sohn,Da-Yong Zhou 과학기술정책연구원 2016 STI Policy Review Vol.7 No.2

This study aims to identify the chronological trajectory of university science parks (USPs) in China and to discuss the roles of government-driven science and technology (S&T) policies in the development of USPs and the future directions of these entities. Our study shows that USPs in China have undergone two development waves: The first from the late 1980s to the late 1990s, when research universities expected to directly participate in economic activities, and the second from 2000 when the Ministry of Science and Technology (MOST) and the Ministry of Education (MOE) jointly enacted the Proposed Regulation of State-level USPs Management to guide and regulate the development of USPs. The development trajectory highlights that USPs are effective platforms that link scientific research, knowledge spillovers and industrial system. However, Chinese USPs still need to confront some conundrums which may influence the processes and outcomes of UILs. Finally, we also summarize the major issues inherent in the development of USPs to guide policymakers to enact more effective policies.

Predicting Successful Recanalization in Patients with Native Coronary Chronic Total Occlusion: The Busan CTO Score

Jin, Cai De,Kim, Moo Hyun,Kim, Soo Jin,Lee, Kwang Min,Kim, Tae Hyung,Cho, Young-Rak,Serebruany, Victor L. S. Karger AG 2017 Cardiology Vol.137 No.2

<P><B><I>Background:</I></B> The optimal strategy to manage chronic total occlusion (CTO) remains unclear. The Japanese CTO multicenter registry (J-CTO) score is an established tool for predicting successful recanalization. However, it does not take into account nonangiographic predictors for final technique success. In the present study, we designed and tested a scoring model called the Busan single-center CTO registry (B-CTO) score combining clinical and angiographic characteristics to predict successful CTO recanalization in Korean patients. <B><I>Methods:</I></B> Prospectively enrolled CTO patients (<I>n</I> = 438) undergoing coronary intervention (1999-2015) were assessed. The B-CTO score comprises 6 independent predictors: age 60-74 years and lesion length ≥20 mm were assigned 1 point each, while age ≥75 years, female gender, lesion location in the right coronary artery, blunt stump, and bending >45° were assigned 2 points each. For each predictor, the points assigned were based on the associated odds ratio by multivariate analysis. The lesions were classified into 4 groups according to the summation of points scored to assess the probability of successful CTO recanalization: easy (score 0-1), intermediate (score 2-3), difficult (score 4-5), and very difficult (score ≥6). CTO opening was designated as the primary endpoint regardless of the interventional era or the skill of the operator. <B><I>Results:</I></B> The final success rate for B-CTO was 81.1%. The probability of successful recanalization for patient groups classified as easy (<I>n</I> = 64), intermediate (<I>n</I> = 148), difficult (<I>n</I> = 134), and very difficult (<I>n</I> = 92) was 95.3, 86.5, 79.1 and 65.2%, respectively (<I>p</I> for trend <0.001). When compared to the J-CTO, the B-CTO score demonstrated a significant improvement in discrimination as indicated by the area under the receiver-operator characteristic curve (AUC 0.083; 95% CI 0.025-0.141), with a positive integrated discrimination improvement of 0.042 and a net reclassification improvement of 56.0%. <B><I>Conclusions:</I></B> The B-CTO score has been designed and validated in Korean patients with native coronary CTO and is an improved tool for predicting successful recanalization. Wider application of the B-CTO score remains to be explored.</P>

Chinese Policy to Stimulate University - Industry Linkages in Nanjing

De-Jin Su,Dong-Won Sohn,Sunwoo Sohn 과학기술정책연구원 2013 STI Policy Review Vol.4 No.2

Rapid changes in the economic, social and academic environments often provide opportunities to develop new and advanced technologies. In China, recent literature on the role of universities suggests that university - industry linkages (UILs) play a substantial role in the development of high-tech industries. Since 1979 when the country became more open and underwent economic reform, Chinese central authorities, local governments, and universities have continued to set up various science and technology (S&T) policies to stimulate UILs, contributing to China’s technological progress and economic growth. This study examines the role of S&T policies on UILs such as transfer of technology, joint research, and spin-off creation with a particular focus on Nanjing University (NJU) in Jiangsu Province. Nanjing has over 53 universities, ranking it behind Beijing and Shanghai in terms of S&T and higher education opportunities. By adopting “institutional methodology,” this study contends that UILs not only benefit universities and industry but also society in terms of job training, consulting activities, joint research, R&D results commercialization, patent licensing, new business creation, and other aspects. Finally, we suggest that the Chinese experience, though with some problems, might enhance our understanding of how to stimulate UILs through the arrangement of various S&T policies.

Study on the bonding mechanism of particleboard made from Laccase-treated Moso bamboo powder

( Jin Chun De ) 韓國木材工學會 2010 한국목재공학회 학술발표논문집 Vol.2010 No.-

Regional Cooperation in Northeast Asia and the Role of Japan

Jin, Xi-de 경희대학교 아태지역연구원 1996 아태연구 Vol.3 No.-

Reactive oxygen species free radical treatment of bamboo flour and its effects on the chemical structure and board bonding strength

( Jin Wang ),( Chun De Jin ),( Jian Gang Song ),( Wei Yang ),( Yuan Lin Cao ) 한국목재공학회 2012 한국목재공학회 학술발표논문집 Vol.2012 No.-