Modeling identification and control of a 6-DOF active vibration isolation system driving by voice coil motors with a Halbach array magnet

Dawei Jiang,Jiasheng Li,Xingzhan Li,Chaodong Deng,Pinkuan Liu 대한기계학회 2020 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.34 No.2

The sheet current model considering the end effects is applied to design a sixdegree-of-freedom (6-DOF) active vibration isolation system (AVIS) driving by the voice coil motors (VCMs). Compared with the charge model, the sheet current method represents higher calculation efficiency. Then, the hybrid functions (HFs) are employed to identify the multi-input and multi-output (MIMO) AVIS, and the identification parameters are used to design the controller. To promote the control performance, the composite nonlinear feedback (CNF) controller is designed based on the identification parameters. Consequently, the simulations and experiments are carried out, compared with PID controller, the CNF controller shows quicker response and smaller overshoot, i.e., the CNF controller achieves the dynamic damping ratio during the control. Thus, the experimental results demonstrate the better performance of the CNF controller.

Dec2 inhibits macrophage pyroptosis to promote periodontal homeostasis

Dawei He,Xiaoyan Li,Fengzhu Zhang,Chen Wang,Yi Liu,Ujjal K. Bhawal,Jiang Sun 대한치주과학회 2022 Journal of Periodontal & Implant Science Vol.52 No.1

Purpose: Macrophages play crucial roles as early responders to bacterial pathogens and promote/ or impede chronic inflammation in various tissues. Periodontal macrophageinduced pyroptosis results in physiological and pathological inflammatory responses. The transcription factor Dec2 is involved in regulating immune function and inflammatory processes. To characterize the potential unknown role of Dec2 in the innate immune system, we sought to elucidate the mechanism that may alleviate macrophage pyroptosis in periodontal inflammation. Methods: Porphyromonas gingivalis lipopolysaccharide (LPS) was used to induce pyroptosis in RAW 264.7 macrophages. Subsequently, we established an LPS-stimulated Dec2 overexpression cellular model in macrophages. Human chronic periodontitis tissues were employed to evaluate potential changes in inflammatory marker expression and pyroptosis. Finally, the effects of Dec2 deficiency on inflammation and pyroptosis were characterized in a P. gingivalis-treated experimental periodontitis Dec2-knockout mouse model. Results: Macrophages treated with LPS revealed significantly increased messenger RNA expression levels of Dec2 and interleukin (IL)-1β. Dec2 overexpression reduced IL-1β expression in macrophages treated with LPS. Overexpression of Dec2 also repressed the cleavage of gasdermin D (GSDMD), and the expression of caspase-11 was concurrently reduced in macrophages treated with LPS. Human chronic periodontitis tissues showed significantly higher gingival inflammation and pyroptosis-related protein expression than non-periodontitis tissues. In vivo, P. gingivalis-challenged mice exhibited a significant augmentation of F4/80, tumor necrosis factor-α, and IL-1β. Dec2 deficiency markedly induced GSDMD expression in the periodontal ligament of P. gingivalis-challenged mice. Conclusions: Our findings indicate that Dec2 deficiency exacerbated P. gingivalis LPS-induced periodontal inflammation and GSDMD-mediated pyroptosis. Collectively, our results present novel insights into the molecular functions of macrophage pyroptosis and document an unforeseen role of Dec2 in pyroptosis.

Dec2 inhibits macrophage pyroptosis to promote periodontal homeostasis

He, Dawei,Li, Xiaoyan,Zhang, Fengzhu,Wang, Chen,Liu, Yi,Bhawal, Ujjal K.,Sun, Jiang Korean Academy of Periodontology 2021 Journal of Periodontal & Implant Science Vol.51 No.-

Purpose: Macrophages play crucial roles as early responders to bacterial pathogens and promote/ or impede chronic inflammation in various tissues. Periodontal macrophage-induced pyroptosis results in physiological and pathological inflammatory responses. The transcription factor Dec2 is involved in regulating immune function and inflammatory processes. To characterize the potential unknown role of Dec2 in the innate immune system, we sought to elucidate the mechanism that may alleviate macrophage pyroptosis in periodontal inflammation. Methods: Porphyromonas gingivalis lipopolysaccharide (LPS) was used to induce pyroptosis in RAW 264.7 macrophages. Subsequently, we established an LPS-stimulated Dec2 overexpression cellular model in macrophages. Human chronic periodontitis tissues were employed to evaluate potential changes in inflammatory marker expression and pyroptosis. Finally, the effects of Dec2 deficiency on inflammation and pyroptosis were characterized in a P. gingivalis-treated experimental periodontitis Dec2-knockout mouse model. Results: Macrophages treated with LPS revealed significantly increased messenger RNA expression levels of Dec2 and interleukin (IL)-1β. Dec2 overexpression reduced IL-1β expression in macrophages treated with LPS. Overexpression of Dec2 also repressed the cleavage of gasdermin D (GSDMD), and the expression of caspase-11 was concurrently reduced in macrophages treated with LPS. Human chronic periodontitis tissues showed significantly higher gingival inflammation and pyroptosis-related protein expression than non-periodontitis tissues. In vivo, P. gingivalis-challenged mice exhibited a significant augmentation of F4/80, tumor necrosis factor-α, and IL-1β. Dec2 deficiency markedly induced GSDMD expression in the periodontal ligament of P. gingivalis-challenged mice. Conclusions: Our findings indicate that Dec2 deficiency exacerbated P. gingivalis LPS-induced periodontal inflammation and GSDMD-mediated pyroptosis. Collectively, our results present novel insights into the molecular functions of macrophage pyroptosis and document an unforeseen role of Dec2 in pyroptosis.

Restoration of Ghost Imaging in Atmospheric Turbulence Based on Deep Learning

Chenzhe Jiang,Banglian Xu,Leihong Zhang,Dawei Zhang 한국광학회 2023 Current Optics and Photonics Vol.7 No.6

Ghost imaging (GI) technology is developing rapidly, but there are inevitably some limitations such as the influence of atmospheric turbulence. In this paper, we study a ghost imaging system in atmospheric turbulence and use a gamma-gamma (GG) model to simulate the medium to strong range of turbulence distribution. With a compressed sensing (CS) algorithm and generative adversarial network (GAN), the image can be restored well. We analyze the performance of correlation imaging, the influence of atmospheric turbulence and the restoration algorithm’s effects. The restored image’s peak signal-to-noise ratio (PSNR) and structural similarity index map (SSIM) increased to 21.9 dB and 0.67 dB, respectively. This proves that deep learning (DL) methods can restore a distorted image well, and it has specific significance for computational imaging in noisy and fuzzy environments.

Rapid screening and identification of metabolites of quercitrin produced by the human intestinal bacteria using ultra performance liquid chromatography/quadrupole-time-of-flight mass spectrometry

Shu Jiang,Jing Yang,Dawei Qian,Jianming Guo,Er-xin Shang,Jin-ao Duan,Jun Xu 대한약학회 2014 Archives of Pharmacal Research Vol.37 No.2

Ultra performance liquid chromatography/quadrupole-time-of-flight mass spectrometry (UPLC/Q-TOF MS)technique combined with MetabolynxTM software was usedfor analysis of the metabolites of quercitrin by the isolatedhuman intestinal bacteria from the human feces. Four metabolitesof quercitrin were detected and tentatively identifiedbased on the characteristics of their protonated ions. Themetabolites were metabolized by four main metabolic pathwaysincluding hydroxylation, demethylation, deglycosylationand ring-cleavage. Quercitrin was metabolized to the hydroxyquercitrinand desmethylquercitrin by themajority of theisolated intestinal bacteria such as Bacteroides sp. 54, and wasdegraded to the deglycosylated product quercetin by rhamnosidaseand further ring-cleavage metabolite 3,4-dihydroxybenzoicacid by the minority of the isolated bacteria such asBacteroides sp. 45. The metabolic pathways and most of themetabolites of quercitrin were reported for the first time.

The Two Echelon Open Vehicle Routing Problem: Optimization of Crowdshipping Based Parcel Delivery

Xue Wu,Dawei Hu,Bingshan Ma,Ruisen Jiang 대한토목학회 2022 KSCE Journal of Civil Engineering Vol.26 No.9

Crowdshipping is a revolutionary concept of the sharing economy. In this study, two carriers are used to perform the following expedition: the truck starts from the depot to complete part of the deliveries, shares part of the load with the crowdshipper at the relay point, and the private driver selected as the crowdshipper continues from that point onward. This study proposes the two-echelon open vehicle routing problem with crowdshipping (2EOVRP-CS) and formulates a mathematical model to determine the crowdshipper, parcel relay location, truck route, and crowdsource route. A tangible nested genetic algorithm (NGA) is proposed, and its efficiency is demonstrated by comparison with CPLEX and genetic algorithm (GA). A real case study is investigated in Xi'an city to test the applicability of the proposed model. The results show that using crowdshipping instead of truck delivery alone can save approximately 14% of the total cost and 26% of truck vehicle miles traveled (VMT). Moreover, several sensitivity analyses are performed. The results show that crowdshipping is sensitive to the detour limit and the time value of carriers. For the detour limit, after the acceptable detour distance increases by 8%, the total cost can be reduced by up to 5.94%.

Three-Dimensional Analysis of Chloroplast Structures Associated with Virus Infection

Jin, Xuejiao,Jiang, Zhihao,Zhang, Kun,Wang, Pengfei,Cao, Xiuling,Yue, Ning,Wang, Xueting,Zhang, Xuan,Li, Yunqin,Li, Dawei,Kang, Byung-Ho,Zhang, Yongliang American Society of Plant Biologists 2018 Plant Physiology Vol.176 No.1

<P>Three-dimensional visualization identifies structural remodeling in chloroplasts during barley stripe mosaic virus infection.</P><P>Chloroplasts are multifunctional organelles whose morphology is affected by environmental stresses. Although the three-dimensional (3D) architecture of thylakoid membranes has been reported previously, a 3D visualization of chloroplast under stress has not been explored. In this work, we used a positive-strand RNA ((+)RNA) virus, barley stripe mosaic virus (BSMV) to observe chloroplast structural changes during infection by electron tomography. The analyses revealed remodeling of the chloroplast membranes, characterized by the clustering of outer membrane-invaginated spherules in inner membrane-derived packets. Diverse morphologies of cytoplasmic invaginations (CIs) were evident with spherules at the periphery and different sized openings connecting the CIs to the cytoplasm. Immunoelectron microscopy of these viral components verified that the aberrant membrane structures were sites for BSMV replication. The BSMV αa replication protein localized at the surface of the chloroplasts and played a prominent role in eliciting chloroplast membrane rearrangements. In sum, our results have revealed the 3D structure of the chloroplasts induced by BSMV infection. These findings contribute to our understanding of chloroplast morphological changes under stress conditions and during assembly of plant (+)RNA virus replication complexes.</P>

A New Way to Prepare MoO₃/C as Anode of Lithium ion Battery for Enhancing the Electrochemical Performance at Room Temperature

Yu, Zhian,Jiang, Hongying,Gu, Dawei,Li, Jishu,Wang, Lei,Shen, Linjiang The Korean Electrochemical Society 2016 Journal of electrochemical science and technology Vol.7 No.2

Composited molybdenum oxide and amorphous carbon (MoO₃/C) as anode material for lithium ion batteries has been successfully synthesized by calcining polyaniline (PANI) doped with ammonium heptamolybdate tetrahydrate (AMo). The as prepared electrode material was characterized by X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FT-IR) and field emission scanning electron microscopy (FE-SEM). The electrochemical performance of the anode was investigated by galvanostatic charge/discharge, cyclic voltammetry (CV), and electrochemical impedance spectroscopy (EIS). The MoO₃/C shows higher specific capacity, better cyclic performance and rate performance than pristine MoO₃ at room temperature. The electrochemical of MoO₃/C properties at various temperatures were also investigated. At elevated temperature, MoO₃/C exhibited higher specific capacity but suffered rapidly declines. While at low temperature, the electrochemical performance was mainly limited by the low kinetics of lithium ion diffusion and the high charge transfer resistance.

Effects of rehydration on physiological and transcriptional responses of a water-stressed rhizobium

Zhu Jie,Jiang Xin,Guan Dawei,Kang Yaowei,Li Li,Cao Fengming,Zhao Baisuo,Ma Mingchao,Zhao Ji,Li Jun 한국미생물학회 2022 The journal of microbiology Vol.60 No.1

As a microsymbiont of soybean, Bradyrhizobium japonicum plays an important role in symbiotic nitrogen fixation and sustainable agriculture. However, the survival of B. japonicum cells under water-deplete (e.g., drought) and water-replete (e.g., flood) conditions is a major concern affecting their nitrogen-fixing ability by establishing the symbiotic relationship with the host. In this study, we isolated a water stress tolerant rhizobium from soybean root nodules and tested its survival under water-deplete conditions. The rhizobium was identified as Bradyrhizobium japonicum and named strain 5038. Interestingly, both plate counting and live/dead fluorescence staining assays indicate that a number of viable but non-culturable cells exist in the culture medium upon the rehydration process which could cause dilution stress. Bradyrhizobium japonicum 5038 cells increased production of exopolysaccharide (EPS) and trehalose when dehydrated, suggesting that protective responses were stimulated. As expected, cells reduced their production upon the subsequent rehydration. To examine differential gene expression of B. japonicum 5038 when exposed to water-deplete and subsequent waterreplete conditions, whole-genome transcriptional analysis was performed under 10% relative humidity (RH), and subsequent 100% RH, respectively. A total of 462 differentially expressed genes (DEGs, > 2.0-fold) were identified under the 10% RH condition, while 3,776 genes showed differential expression during the subsequent rehydration (100% RH) process. Genes involved in signal transduction, inorganic ion transport, energy production and metabolisms of carbohydrates, amino acids, and lipids were far more up-regulated than downregulated in the 10% RH condition. Notably, trehalose biosynthetic genes (otsAB, treS, and treYZ), genes ligD, oprB, and a sigma factor rpoH were significantly induced by 10% RH. Under the subsequent 100% RH condition, genes involved in transcription, translation, cell membrane regulation, replication and repair, and protein processing were highly up-regulated. Interestingly, most of 10%-RH inducible genes displayed rehydration-repressed, except three genes encoding heat shock (Hsp20) proteins. Therefore, this study provides molecular evidence for the switch of gene expression of B. japonicum cells when encountered the opposite water availability from water-deplete to water-replete conditions.

Radiolabeled polyoxometalate clusters: Kidney dysfunction evaluation and tumor diagnosis by positron emission tomography imaging

Ni, Dalong,Jiang, Dawei,Im, Hyung-Jun,Valdovinos, Hector F.,Yu, Bo,Goel, Shreya,Barnhart, Todd E.,Huang, Peng,Cai, Weibo Elsevier 2018 Biomaterials Vol.171 No.-

<P><B>Abstract</B></P> <P>Radiolabeled nanoprobes for positron emission tomography (PET) imaging has received special attention over the past decade, allowing for sensitive, non-invasive, and quantitative detection of different diseases. The rapidly renal clearable nanomaterials normally suffer from a low accumulation in the tumor through the enhanced permeability and retention (EPR) effect due to the rapidly reduced concentration in the blood circulation after renal clearance. It is highly important to design radiolabeled nanomaterials which can meet the balance between the rapid renal clearance and strong EPR effect within a suitable timescale. Herein, renal clearable polyoxometalate (POM) clusters of ultra-small size (∼1 nm in diameter) were readily radiolabeled with the oxophilic <SUP>89</SUP>Zr to obtain <SUP>89</SUP>Zr-POM clusters, which may allow for efficient staging of kidney dysfunction in a murine model of unilateral ureteral obstruction (UUO). Furthermore, the as-synthesized clusters can accumulate in the tumor through EPR effect and self-assemble into larger nanostructures in the acidic tumor microenvironment for enhanced tumor accumulation, offering an excellent balance between renal clearance and EPR effect.</P> <P><B>Graphical abstract</B></P> <P>Herein, renal clearable polyoxometalate clusters (POM) were readily radiolabeled with the oxophilic <SUP>89</SUP>Zr to allow for efficient staging of kidney dysfunction in a murine model. Furthermore, the as-synthesized clusters can accumulate in the tumor through enhanced permeability and retention (EPR) effect and self-assemble into larger nanostructures in the acidic tumor microenvironment for enhanced tumor accumulation, offering an excellent balance between renal clearance and EPR effect.</P> <P>[DISPLAY OMISSION]</P>