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RISS 인기검색어
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- 저자 : Dai Di Fan (검색결과 5 건)
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Shang, Longan,Fan, Dai Di,Kim, Moon-Il,Choi, Jin-dal-rae,Chang, Ho-Nam Korean Society for Biotechnology and Bioengineerin 2007 Biotechnology and Bioprocess Engineering Vol.12 No.4
High cell density culturing has been conducted for the production of poly(3-hydroxybutyrate) fed-batch cultures of Ralstonia eutropha with phosphate limitation. It was found that a high glucose concentration inhibited the synthesis of P(3HB) in the high cell density culture of R. eutropha. Although a low glucose concentration can trigger the synthesis of P(3HB) in a manner similar to that of phosphate limitation, it also limited both the P(3HB) synthesis and the cell growth, and led to a low P(3HB) productivity because glucose is the sole carbon source in this reaction. An unstructured model was proposed for predicting the cell growth and P(3HB) synthesis in high cell density cultures of R. eutropha, where the phosphate concentration played a key role in the accumulation of P(3HB) and in cell growth. Good agreements were found between the experimental data and model predictions. The results of simulation showed that the final P(3HB) concentration would decrease more than 25% when the glucose was concentration increased to 40 g/L, and indicated that the optimal glucose concentration for P(3HB) production by high cell density cultures of R. eutropha was around 9g/L.
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Lina Wang,Dai Di Fan,Jing He,Zhongcheng Lv,Chen-Hui Zhu 한국생물공학회 2014 Biotechnology and Bioprocess Engineering Vol.19 No.5
Recombinant human full-length mature collagenα1 (III) chain (rhCOL3A1) was secreted by Pichia pastorisGS115, using the Saccharmyces cerevisiae á-mating factorprepro signal, and the theoretical molecular weight ofrhCOL3A1 was 95.344 kDa. The gene cloned from humanplacenta, was designed and cloned into expression vectorpPIC9K under the control of a strong inducible promoterAOX1.The expression stage of rhCOL3A1 was sensitiveto different carbon ratios through mixed fermentation. LCMS/MS analysis and western blotting demonstrated thatthe recombinant human full-length mature collagen α1(III) gene was successfully expressed in P. pastoris GS115during the methanol induction stage. Furthermore, aneffective strategy of mixed fermentation was established toexpress rhCOL3A1 in shake flash. Compared to singlecarbon induction, when induced with mixed carbon at theration of 0.8 (glycerol/methanol), the time corresponding tothe highest yield of rhCOL3A1 (1.27 g/L) was drasticallyreduced by 50%. The same conclusion was observed fromRT-qPCR. Consequently, a new strategy which was moretime-saving and effective was provided for the large-scaleproducing the full-length mature rhCOL3A1.
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Lei Chi,Dai-Di Fan,Xiao-Xuan Ma,Yan-E Luo,Chen-Hui Zhu 한국생물공학회 2011 Biotechnology and Bioprocess Engineering Vol.16 No.3
To increase the biomass and production of recombinant human-like collagen (RHLC), the effect of controlled fermentor pressure during fed-batch cultivation was investigated using recombinant Escherichia coli producing RHLC. This study focused primarily on the effects of the fermentor pressure on the oxygen transfer capacity. A twostep exponential feeding strategy was used to control the specific growth rate at 0.2 and 0.1/h in the fed-batch and induction phase, respectively. A kinetic model of cell growth was developed, and the specific growth rate, specific glucose uptake rate, concentration of extracellular DNA, and percentage of plasmid loss were calculated and detected. The results demonstrated that increasing the fermentor pressure was an effective way of avoiding the oxygen transfer capacity limitation, and an increase in the dissolved CO2 content did not affect the growth of the recombinant E. coli BL21strain. At the end of the fermentation process, the cell density (represented by the dry cell weight, DCW) reached 77.3 g/L, and the RHLC concentration reached 14.1 g/L. In addition,the oxygen transfer capacity (KLaC^*) decreased drastically at approximately 5 h after induction. This is probably because of the increased concentration of extracellular DNA due to cell lysis, indicating that the cells needed to be harvested.
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장호남,김문일,Longan Shang,Dai Di Fan,Jin-dal-rae Choi 한국생물공학회 2007 Biotechnology and Bioprocess Engineering Vol.12 No.4
High cell density culturing has been conducted for the production of poly(3-hydroxybutyrate) fed-batch cultures of Ralstonia eutropha with phosphate limitation. It was found that a high glucose concentration inhibited the synthesis of P(3HB) in the high cell density culture of R. eutropha. Although a low glucose concentration can trigger the synthesis of P(3HB) in a manner similar to that of phosphate limitation, it also limited both the P(3HB) synthesis and the cell growth, and led to a low P(3HB) productivity because glucose is the sole carbon source in this reaction. An unstructured model was proposed for predicting the cell growth and P(3HB) synthesis in high cell density cultures of R. eutropha, where the phosphate concentration played a key role in the accumulation of P(3HB) and in cell growth. Good agreements were found between the experimental data and model predictions. The results of simulation showed that the final P(3HB) concentration would decrease more than 25% when the glucose was concentration increased to 40 g/L, and indicated that the optimal glucose concentration for P(3HB) production by high cell density cultures of R. eutropha was around 9 g/L.
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Ling Wang,Dong-yang Dai,Xia Wu,Yun-yan Sheng,Peng Ji,Dan-dan Li,Fan Zhang,Di Wang 한국원예학회 2021 원예과학기술지 Vol.39 No.5
The male sterile plants have higher heterosis in the production of hybrid seeds. The ABORTED MICROSPORES (AMS) gene has been demonstrated to be a candidate gene for ms-5. However, the genetic mechanism underlying AMS-mediated male sterility (MS) regulatory networks in melon (Cucumis melo L.) is still not clearly understood. In the present study, we used transcriptome sequencing analysis, yeast hybridization technology, quantitative real-time polymerase chain reaction (qRT-PCR), and bioinformatics analyzed to systematically investigate the AMS-mediated MS regulatory networks in melon. A set of 15 proteins interacting with AMS, including the C. melo L. Zinc Ribbon protein 1 (CmZR1) gene, was identified using the yeast one-hybrid (Y1H) system and further confirmed using the yeast two-hybrid (Y2H) assay. The interaction of the CmZR1 protein with the C. melo L. Pectin Methylesterase Inhibitor 1 (CmPMEI1) protein was identified and further verified by the glutathione S-transferase (GST) pull-down technique. Bioinformatics analyzed the physical and chemical properties, gene structure, and kinship of the melon PMEI family. We proposed a partial regulatory network for melon MS in which the interaction of CmPMEI1 protein with CmZR1 protein regulates the expression of the AMS gene for pollen abortion. These findings provide important information for increasing the understanding of the molecular mechanism of the MS regulatory network in melon.
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