http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
Characterization of Antibacterial Strains against Kiwifruit Bacterial Canker Pathogen
Kim, Min-Jung,Chae, Dae-Han,Cho, Gyeongjun,Kim, Da-Ran,Kwak, Youn-Sig The Korean Society of Plant Pathology 2019 Plant Pathology Journal Vol.35 No.5
Kiwifruit (Actinidia spp.) is an economically important crop and a bacterial canker disease, caused by Pseudomonas syringae pv. actinidiae (Psa), is the most destructive disease in kiwifruit production. Therefore, prevent and control of the disease is a critical issue in kiwifruit industry worldwide. Unfortunately, there is no reliable control methods have been developed. Recently, interest in disease control using microbial agents is growing. However, kiwifruit microbiota and their roles in the disease control is mainly remaining unknown. In this study, we secured bacterial libraries from kiwifruit ecospheres (rhizosphere, endospere, and phyllosphere) and screened reliable biocontrol strains against Psa. As the results, Streptomyces racemochromogenes W1SF4, Streptomyces sp. W3SF9 and S. parvulus KPB2 were selected as anti-Psa agents from the libraries. The strains showed forcible antibacterial activity as well as exceptional colonization ability on rhizosphere or phyllosphere of kiwifruit. Genome analyses of the strains suggested that the strains may produce several anti-Psa secondary metabolites. Our results will contribute to develop biocontrol strains against the kiwifruit canker pathogen and the disease management strategies.
Kim Sung Ran,Kang Hyeon Jeong,Jeong Hye Rin,Jang Su Yeon,Lee Jae Eun,Kim Da Eun,Lee Hae Ry,Cho Min Hee,Noh Ji Yun,Cheong Hee Jin,Kim Woo Joo,Song Joon Young 대한의학회 2022 Journal of Korean medical science Vol.37 No.35
The omicron variant of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is known to have high infectivity and is more likely to evade vaccine immunity. However, booster vaccination is expected to strengthen cross-reactive immunity, thereby increasing the vaccine effectiveness (VE). This study aimed to evaluate the relative VE of the 3-dose (booster) vaccination compared with the 2-dose primary series vaccination in healthcare workers during omicron variant-dominant periods. During the omicron-dominant period from February 1, 2022 to February 28, 2022, a 1:1 matched case-control study was conducted. Healthcare workers with positive SARS-CoV-2 test results were classified as positive cases, whereas those with negative results served as controls. Compared with the 2-dose primary series vaccination, booster vaccination with mRNA vaccine showed moderate VE (53.1%). However, in multivariate analysis including the time elapsed after vaccination, the significant VE disappeared, reflecting the impact of recent vaccination rather than the third dose itself.
Da-Ran Kim,Youn-Sig Kwak 한국농약과학회 2021 한국농약과학회 학술발표대회 논문집 Vol.2021 No.11
The strawberry is one of the major economic crops in Korea. Gray mold disease which caused by the Botrytis cinerea and Blossom blight disease occurred by Cladosporium cladosporioides which are known to be the most problematic for strawberry anthosphere. In previously study, Streptomyces globisporus SP6C4 was isolated at strawberry anthosphere and suggested a majority antifungal compound was conprimycin and grisin. The S. globisporus SP6C4 has study of antifungal compound, which metabolite to be novel compound. These compound is secondary metabolite, which is not necessary but do adapt ecology system with some metabolite. In a previous survey from 2017 to 2018, strawberry cultivation of the plant decreased Gray mold and Blossom blight disease incidence and severity. In this study, control efficacy of S. globisporus SP6C4 was evaluated on the two different disease of strawberry. Two of different disease of B. cinerea and C. cladosporioides were decrease to the disease severity, also more control the mummification value on fruits. In most things, anthosphere diseases were the most important to decrease the disease severity to improve productivity in cultivation system.
A Genome-Wide Analysis of Antibiotic Producing Genes in Streptomyces globisporus SP6C4
Da-Ran Kim,Youn-Sig Kwak 한국식물병리학회 2021 Plant Pathology Journal Vol.37 No.4
Soil is the major source of plant-associated microbes. Several fungal and bacterial species live within plant tissues. Actinomycetes are well known for producing a variety of antibiotics, and they contribute to improving plant health. In our previous report, Streptomyces glo- bisporus SP6C4 colonized plant tissues and was able to move to other tissues from the initially colonized ones. This strain has excellent antifungal and antibacterial activities and provides a suppressive effect upon various plant diseases. Here, we report the genome-wide analysis of antibiotic producing genes in S. globisporus SP6C4. A total of 15 secondary metabolite biosynthetic gene clusters were predicted using antiSMASH. We used the CRISPR/Cas9 mutagenesis system, and each biosynthetic gene was predicted via protein basic local alignment search tool (BLAST) and rapid annotation using subsystems technology (RAST) server. Three gene clusters were shown to exhibit antifungal or anti- bacterial activity, viz. cluster 16 (lasso peptide), cluster 17 (thiopeptide-lantipeptide), and cluster 20 (lanti- peptide). The results of the current study showed that SP6C4 has a variety of antimicrobial activities, and this strain is beneficial in agriculture.
Copine1 regulates neural stem cell functions during brain development
Kim, Tae Hwan,Sung, Soo-Eun,Cheal Yoo, Jae,Park, Jae-Yong,Yi, Gwan-su,Heo, Jun Young,Lee, Jae-Ran,Kim, Nam-Soon,Lee, Da Yong Elsevier 2018 Biochemical and biophysical research communication Vol.495 No.1
<P><B>Abstract</B></P> <P>Copine 1 (CPNE1) is a well-known phospholipid binding protein in plasma membrane of various cell types. In brain cells, CPNE1 is closely associated with AKT signaling pathway, which is important for neural stem cell (NSC) functions during brain development. Here, we investigated the role of CPNE1 in the regulation of brain NSC functions during brain development and determined its underlying mechanism. In this study, abundant expression of CPNE1 was observed in neural lineage cells including NSCs and immature neurons in human. With mouse brain tissues in various developmental stages, we found that CPNE1 expression was higher at early embryonic stages compared to postnatal and adult stages. To model developing brain in vitro, we used primary NSCs derived from mouse embryonic hippocampus. Our in vitro study shows decreased proliferation and multi-lineage differentiation potential in CPNE1 deficient NSCs. Finally, we found that the deficiency of CPNE1 downregulated mTOR signaling in embryonic NSCs. These data demonstrate that CPNE1 plays a key role in the regulation of NSC functions through the activation of AKT-mTOR signaling pathway during brain development.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Copine1 is highly expressed in neural lineage cells at early developmental stages. </LI> <LI> Copine1 regulates the proliferation of embryonic neural stem cells (NSCs). </LI> <LI> Copine1 regulates the differentiation of NSCs into neuronal and glial lineages. </LI> <LI> mTOR signaling pathway is involved in Copine1-meidated neural development. </LI> </UL> </P>