PF4V1 affects the progression of oral squamous cell carcinoma by regulating Wnt/ β‑catenin pathway and angiogenesis

Cuiping Li,Weidong Jiang,Yang Zhou,Xuanping Huang,Nuo Zhou 한국응용생명화학회 2020 Applied Biological Chemistry (Appl Biol Chem) Vol.63 No.2

Platelet factor-4 variant 1 (PF4V1) was recently described as a natural non-allelic gene variant of platelet factor-4 (PF4), which has been closely associated with the growth and metastasis of various cancers. Our previous research showed that PF4V1 was related to oral squamous cells carcinoma (OSCC) metastasis. Howerver, it is still not clear about the functional role of PF4V1 in OSCC. In this study, stably transfected cell lines were constructed and the expression level of PF4V1 was verified by real‐time polymerase chain reaction (RT-PCR) and western blot. The effect of PF4V1 on proliferation, migration, invasion, and apoptosis of oral cancer (OC) cells were detected. Moreover, a xenograft tumor model was constructed to evaluate the effect of PF4V1 on OSCC in vivo. Indicators of Wnt/β-catenin, angiogenesis and epithelial-mesenchymal transition (EMT) pathways were also examined. Stable cell lines with overexpression and inhibited expression of PF4V1 were constructed successfully. After stable transfection, PF4V1 significantly promoted the proliferation, migration, and invasion of OC cells in vitro, and their tumor formation in vivo. Furthermore, PF4V1 remarkably promoted the expression of β-catenin, VEGF, and FGF but suppressed the expression of GSK-3β. There was no statistically significant correlation between PF4V1 and EMT pathway. This study provides evidence that PF4V1 promotes the proliferation, migration, invasion and tumor formation of OC cells by regulating the Wnt/β-catenin pathway and angiogenesis. Our findings suggest that PF4V1 could be a very promising target of OSCC therapy in the future.

A Method of Zero self-modification and Temperature Compensation for Indoor Air Quality Detection Based on a software Model

Cuiping Li,Jiuqiang Han,Qibin Huang,Xiaoqiang Dong,Xuequan Ding,Yaming Ding,Dianguo Zhang,Ning Mu 제어로봇시스템학회 2012 제어로봇시스템학회 국제학술대회 논문집 Vol.2012 No.10

It is very difficult to apply non-dispersive infrared sensor to detect the indoor air quality and maintain very low zero and temperature drift over long periods. Frequently manual zero setting and calibration are required. To solve the issues of zero and temperature drift of non-dispersive infrared sensor, a software model based on zero gas intensity, reference channels intensity, standard temperature, environmental temperature, temperature drift coefficient, etc. has been established to automatically modify and compensate the zero and temperature drift existing in the long-term continuous operation of the infrared sensor. The test result and long-term application indicate the detection precision of the instrument is less than 5%F.S in various changing environmental conditions. The average detection precision of carbon dioxide has been improved from 9.26% before comprehensive processing to 1.23% after processing, while the average detection precision of methane has been improved from 10.61% before comprehensive processing to 0.70% after processing. As a result, the disadvantages existing in many gas detectors including poor stability and short calibration cycle have been overcome, thus effectively improving the detection precision and stability of the instrument and reducing the maintenance cost.

Investigation on Chain Transfer Reaction of Benzene Sulfonyl Chloride in Styrene Radical Polymerization

Li, Cuiping,Fu, Zhifeng,Shi, Yan The Polymer Society of Korea 2009 Macromolecular Research Vol.17 No.8

The free radical polymerization of styrene was initiated with azobis(isobutyronitrile) in the presence of benzene sulfonyl chloride. Analysis of the terminal structures of the obtained polystyrene with $^1H$ NMR spectroscopy revealed the presence of a phenyl sulfonyl group at the ${\alpha}$-end and a chlorine atom at the ${\omega}$-end of each polystyrene chain. The terminal chlorine atom in the polystyrene chains was further confirmed through atom transfer radical polymerization (ATRP) of styrene and methyl acrylate using the obtained polystyrenes as macroinitiators and CuCl/2,2'-bipyridine as the catalyst system. GPC traces of the products obtained in ATRP at different reaction times were clearly shifted to higher molecular weight direction, indicating that nearly all the macroinitiator chains initiated ATRP of the second monomers. In addition, the number-average molecular weights of the polystyrenes increased directly proportional to the monomer conversions, and agreed well with the theoretical ones.

Bacterial Community Structure and Function Shift in Rhizosphere Soil of Tobacco Plants Infected by Meloidogyne incognita

Wenjie Tong(Wenjie Tong),Junying Li(Junying Li),Wenfeng Cong(Wenfeng Cong),Cuiping Zhang(Cuiping Zhang),Zhaoli Xu(Zhaoli Xu),Xiaolong Chen(Xiaolong Chen),Min Yang(Min Yang),Jiani Liu(Jiani Liu),Lei Yu 한국식물병리학회 2022 Plant Pathology Journal Vol.38 No.6

Root-knot nematode disease is a widespread and catastrophic disease of tobacco. However, little is known about the relationship between rhizosphere bacterial community and root-knot nematode disease. This study used 16S rRNA gene sequencing and PICRUSt to assess bacterial community structure and function changes in rhizosphere soil from Meloidogyne incognita-infected tobacco plants. We studied the rhizosphere bacterial community structure of M. incognita-infected and uninfected tobacco plants through a paired comparison design in two regions of tobacco planting area, Yuxi and Jiuxiang of Yunnan Province, southwest China. According to the findings, M. incognita infection can alter the bacterial population in the soil. Uninfested soil has more operational taxonomic unit numbers and richness than infested soil. Principal Coordinate Analysis revealed clear separations between bacterial communities from infested and uninfested soil, indicating that different infection conditions resulted in significantly different bacterial community structures in soils. Firmicutes was prevalent in infested soil, but Chloroflexi and Acidobacteria were prevalent in uninfested soil. Sphingomonas, Streptomyces, and Bradyrhizobium were the dominant bacteria genera, and their abundance were higher in infested soil. By PICRUSt analysis, some metabolism-related functions and signal transduction functions of the rhizosphere bacterial community in the M. incognita infection-tobacco plants had a higher relative abundance than those uninfected. As a result, rhizosphere soils from tobacco plants infected with M. incognita showed considerable bacterial community structure and function alterations.

Pseudolaric Acid B Inhibits Proliferation, Invasion and Epithelial-to-Mesenchymal Transition in Human Pancreatic Cancer Cell

Xiaoyu Li,Xianzhi Zhao,Wen Song,Zibin Tian,Lin Yang,Qinghui Niu,Qi Zhang,Man Xie,Bin Zhou,Yonghong Xu,Jun Wu,Cuiping Zhang 연세대학교의과대학 2018 Yonsei medical journal Vol.59 No.1

Purpose: This study was aimed to investigate the effect of pseudolaric acid B (PAB) on proliferation, invasion and epithelial-to-mesenchymal transition (EMT) in pancreatic cancer cells and to explore the possible mechanism. Materials and Methods: The pancreatic cancer cell line SW1990 was cultured and treated with PAB dose- and time-dependent manners. Cell proliferation and invasion ability were measured by MTT assay and Matrigel/Transwell test, respectively. Semi-quantitative real-time polymerase chain reaction and Western blotting were conducted to detect the expression of EMT markers and the key molecules. Finally, nude mice subcutaneous transplantation tumor model was used to confirm the therapy efficacy of PAB. Results: PAB could inhibit SW1990 cell proliferation and invasion in time- and dose-dependent manners. Vimentin, fibronectin, N-cadherin, Snail, Slug, YAP, TEAD1, and Survivin were down-regulated (p<0.01), while E-cadherin, caspase-9, MST1, and pYAP were up-regulated (p<0.05). Combined PAB and gemcitabine treatment markedly restricted the tumor growth compared with gencitabin or PAB alone groups. Conclusion: PAB could inhibit the proliferation and invasion ability of pancreatic cancer cells through activating Hippo-YAP pathway and inhibiting the process of EMT.

ORiginal Article : Association of IL28B Genotypes and Baseline Serum Interferon-γ-Inducible-Protein-10 Levels with Treatment Response in Hepatitis C Virus Patients in China

( Renwen Zhang ),( Cuiping Shao ),( Na Huo ),( Minran Li ),( Xiaoyuan Xu ) 대한간학회 2016 Gut and Liver Vol.10 No.3

Background/Aims: Several studies have demonstrated that serum interferon-γ-inducible-protein-10 (IP-10) levels at baseline and single nucleotide polymorphisms (SNPs) near the IL28B gene were associated with viral response and treatment outcomes. Our purpose was to assess the combination of pretreatment IP-10 levels with IL28B SNPs as predictors of treatment response to pegylated interferon α-2a plus ribavirin in patients infected with genotype 1 hepatitis C virus in China. Methods: Seventy-two patients with chronic hepatitis C without fibrosis/cirrhosis were enrolled in the study. The virologic parameters and baseline serum IP-10 levels were determined. IL-28B genotypes were determined by sequencing. Results: In this cohort, serum baseline IP-10 levels lower than 426.7 pg/mL could predict rapid virological response/ sustained virological response (SVR). Patients carrying favorable IL28B SNP genotypes had higher SVRs than did those carrying unfavorable variants (IL28B rs12979860, p=0.002; IL28B rs8099917, p=0.020). Combining both baseline IP- 10 and IL28B SNPs could improve the prediction of SVR in favorable allele carriers of IL28B, rs12979860 CC and rs8099917 TT. Serum baseline IP-10 levels and IL28B genotypes were independent predictors of SVR. Conclusions: Our study shows that the combination of baseline serum IP-10 levels and the determination of IL28B SNPs increase the predictability of SVR rates in this cohort. (Gut Liver 2016;10:446-455)

Hesperetin ameliorates lipopolysaccharide-induced acute lung injury in mice through regulating the TLR4–MyD88–NF-kB signaling pathway

Naigang Wang,Cuiping Geng,Haiyun Sun,Xia Wang,Fangmin Li,Xunchao Liu 대한약학회 2019 Archives of Pharmacal Research Vol.42 No.12

Hesperetin, a major bioflavonoid in sweetoranges and lemons, exerts an anti-inflammatory effect inpulmonary diseases; however, its effect on lipopolysaccharide(LPS)-induced acute lung injury is unclear. This studyinvestigated the effect of hesperetin on LPS-induced lunginflammatory response. Mice were intratracheally instilledwith 5 mg/kg body weight LPS, and then were given hesperetinorally (10, 20, and 30 mg/kg body weight) 1 h later. Hesperetin dramatically suppressed the levels of interleukin-6 and tumor necrosis factor-α, as well as the number ofinflammatory cells in bronchoalveolar lavage fluid. Besides,it reduced lung injury, wet weight/dry weight ratio, andmyeloperoxidase and lactate dehydrogenase activities, andenhanced superoxide dismutase activity. In addition, hesperetinsignificantly downregulated the Toll-like receptor4 (TLR4) and myeloid differentiation factor 88 (MyD88)protein expression and suppressed nuclear factor-kappa B(NF-κB) activation in lung tissue. Together, these resultsindicated that the anti-inflammatory effect of hesperetin isassociated with the TLR4–MyD88–NF-κB pathway, andthat hesperetin shows therapeutic potential for LPS-inducedacute lung injury.

An efficient Agrobacterium-mediated transformation method for aflatoxin generation fungus Aspergillus flavus

Guomin Han,Qian Shao,Cuiping Li,Kai Zhao,Li Jiang,Jun Fan,Haiyang Jiang,Fang Tao 한국미생물학회 2018 The journal of microbiology Vol.56 No.5

Aspergillus flavus often invade many important corps and produce harmful aflatoxins both in preharvest and during storage stages. The regulation mechanism of aflatoxin biosynthesis in this fungus has not been well explored mainly due to the lack of an efficient transformation method for constructing a genome-wide gene mutant library. This challenge was resolved in this study, where a reliable and efficient Agrobacterium tumefaciens-mediated transformation (ATMT) protocol for A. flavus NRRL 3357 was established. The results showed that removal of multinucleate conidia, to collect a homogenous sample of uninucleate conidia for use as the transformation material, is the key step in this procedure. A. tumefaciens strain AGL-1 harboring the ble gene for zeocin resistance under the control of the gpdA promoter from A. nidulans is suitable for genetic transformation of this fungus. We successfully generated A. flavus transformants with an efficiency of ~ 60 positive transformants per 106 conidia using our protocol. A small-scale insertional mutant library (~ 1,000 mutants) was constructed using this method and the resulting several mutants lacked both production of conidia and aflatoxin biosynthesis capacity. Southern blotting analysis demonstrated that the majority of the transformants contained a single T-DNA insert on the genome. To the best of our knowledge, this is the first report of genetic transformation of A. flavus via ATMT and our protocol provides an effective tool for construction of genome-wide gene mutant libraries for functional analysis of important genes in A. flavus.

Calixarene-based chemosensors by means of click chemistry.

Song, Miaomiao,Sun, Zhongyue,Han, Cuiping,Tian, Demei,Li, Haibing,Kim, Jong Seung Wiley-VCH 2014 Chemistry - An Asian Journal Vol.9 No.9

<P>Click chemistry, a new strategy for organic chemistry, has been widely used in the chemical modification of calixarenes because of its reliability, specificity, biocompatibility, and efficiency. Click-derived triazoles also play a critical role in sensing ions and molecules. This in-depth review provides an overview of calixarene-based chemosensors that incorporate click-derived triazoles, and their three characteristics (chromogenic, fluorescence, and wettability) are reviewed.</P>