Aqueous Extracts of Se-Enriched Auricularia auricular Exhibits Antioxidant Capacity and Attenuate Liver Damage in High-Fat Diet/Streptozotocin-Induced Diabetic Mice

Chunyan Wei,Jingjing Wang,Cuicui Duan,Huimei Fan,Xiaoxiao Liu 한국식품영양과학회 2020 Journal of medicinal food Vol.23 No.2

Oxidative stress triggered by hyperglycemia is thought to be a major factor in the development of liver disease during diabetes mellitus (DM). The aim of this study was to determine the antioxidant capacity of aqueous extracts of selenium-enriched Auricularia auricular (AESA) and further investigate the hepatic protection and potential mechanism of AESA in a mouse model of diabetes. An in vitro antioxidant assay confirmed that AESA exhibited better antioxidant capacity characterized by increased reducing power and scavenging capacity of free radicals, such as diphenyl picrylhydrazyl radical, hydroxyl radical, and superoxide radical. The diabetic model was induced by high-fat diet combined with a single injection of streptozotocin in C57BL/6 mice. Our results showed that AESA treatment improved diabetes-induced disorders of lipid metabolisms and alleviated liver damage in diabetic mice. Furthermore, the antioxidant enzyme activities of glutathione peroxidase and catalase in liver were increased and malondialdehyde level was decreased with AESA treatment compared with those in the DM group. In parallel, AESA significantly reduced the contents of tumor necrosis factor-alpha and interleukin-1beta in liver in comparison with the DM group. In addition, western blot results showed that the expression of receptor for advanced glycation end products (RAGE), phospho-c-Jun NH2-terminal kinase, phospho-extracellular signal-regulated kinase, and phospho-p38 kinase were remarkably decreased in AESA treatment group compared with DM group. Taken together, supplementation of AESA may effectively attenuate diabetic hepatopathology by exerting antioxidant function through the RAGE/mitogen-activated protein kinase pathway.

Identification of S-Nitrosylation of Proteins of Helicobacter pylori in Response to Nitric Oxide Stress

Wei Qu,Yabin Zhou,Yundong Sun,Ming Fang,Han Yu,Wenjuan Li,Zhifang Liu,Jiping Zeng,Chunyan Chen,Chengjiang Gao,Jihui Jia 한국미생물학회 2011 The journal of microbiology Vol.49 No.2

Innate and adaptive immune responses are activated in humans when Helicobacter pylori invades the gastric mucosa. Nitric oxide (NO) and reactive nitrogen species are important immune effectors, which can exert their functions through oxidation and S-nitrosylation of proteins. S-nitrosoglutathione and sodium nitroprusside were used as NO donors and H. pylori cells were incubated with these compounds to analyze the inhibitory effect of NO. The suppressing effect of NO on H. pylori has been shown in vitro. Furthermore,the proteins modified by S-nitrosylation in H. pylori were identified through the biotin switch method in association with matrix-assisted laser desorption ionization/time-of-flight tandem mass spectrometry (MALDITOF-MS/MS). Five S-nitrosylated proteins identified were a chaperone and heat-shock protein (GroEL),alkyl hydroperoxide reductase (TsaA), urease alpha subunit (UreA), HP0721, and HP0129. Importantly,S-nitrosylation of TsaA and UreA were confirmed using purified recombinant proteins. Considering the importance of these enzymes in antioxidant defenses, adherence, and colonization, NO may exert its antibacterial actions by targeting enzymes through S-nitrosylation. Identification of protein S-nitrosylation may contribute to an understanding of the antibacterial actions of NO. Our findings provide an insight into potential targets for the development of novel therapeutic agents against H. pylori infection.

Helicobacter pylori Proteins Response to Nitric Oxide Stress

Wei Qu,Yabin Zhou,Chunhong Shao,Yundong Sun,Qunye Zhang,Chunyan Chen,Jihui Jia 한국미생물학회 2009 The journal of microbiology Vol.47 No.4

Helicobacter pylori is a highly pathogenic microorganism with various strategies to evade human immune responses. Nitric oxide (NO) and reactive nitrogen species (RNS) generated via nitric oxide synthase pathway are important effectors during the innate immune response. However, the mechanisms of H. pylori to survive the nitrosative stress are not clear. Here the proteomic approach has been used to define the adaptive response of H. pylori to nitrosative stress. Proteomic analysis showed that 38 protein spots were regulated by NO donor, sodium nitroprusside (SNP). These proteins were involved in protein processing, antioxidation, general stress response, and virulence, as well as some unknown functions. Particularly, some of them were participated in iron metabolism, potentially under the control of ferric uptake regulator (Fur). Real time PCR revealed that fur was induced under nitrosative stress, consistent with our deduction. One stress-related protein up-regulated under nitrosative conditions was thioredoxin reductase (TrxR). Inactivation of fur or trxR can lead to increased susceptivity to nitrosative stress respectively. These studies described the adaptive response of H. pylori to nitric oxide stress, and analyzed the relevant role of Fur regulon and TrxR in nitrosative stress management.

Optimal LQ Tracking Control for Continuous-time Systems with Pointwise Time-varying Input Delay

Chunyan Han,Wei Wang 제어·로봇·시스템학회 2017 International Journal of Control, Automation, and Vol.15 No.5

This paper investigates the linear quadratic (LQ) tracking problem for linear continuous-time systemswith pointwise time-varying input delays. It is assumed that the time-varying input delay takes only one valuefrom a finite set at a given time instant. By defining an indicator, the investigated LQ tracking problem is firstlytransformed into a special optimal control problem for continuous-time systems with multiple delays in a singleinput channel. Then the duality between the LQ tracking for the system with multiple input delay and the smoothingfor an associated backward delay free system is established. Finally, an explicit analytical solution to the proposedLQ tracking problem is presented by solving the smoothing problem.

White Noise Estimators for Networked Systems with Packet Dropouts

Chunyan Han,Wei Wang,Yuan Zhang 제어·로봇·시스템학회 2013 International Journal of Control, Automation, and Vol.11 No.6

This paper studies the optimal and suboptimal deconvolution problems over a network subject to random packet losses, which are modeled by an independent identically distributed Bernoulli process. By the projection formula, an optimal input white noise estimator is first presented with a stochastic Kalman filter. We show that this obtained deconvolution estimator is time-varying, stochastic, and it does not converge to a steady value. Then an alternative suboptimal input white-noise estimator with deterministic gains is developed under a new criterion. The estimator gain and its respective error covariance-matrix information are derived based on a new suboptimal state estimator. It can be shown that the suboptimal input white-noise estimator converges to a steady-state one under appropriate assumptions.

Distributed Observer-based LQ Controller Design and Stabilization for Discrete-time Multi-agent Systems

Chunyan Han,Wei Wang 제어·로봇·시스템학회 2018 International Journal of Control, Automation, and Vol.16 No.4

This paper will investigate the distributed observer-based LQ controller design and stabilization problem for uncoupled identical linear time-invariant multi-agent systems with a performance index coupling the behavior between the multi-agents. A design method is proposed by applying the decomposition of the global discrete-time algebraic Riccati equations. A computationally tractable solution can be obtained by solving four local algebraic Riccati equations which have the same dimensions as a single agent. The stability condition is given in terms of the spectrum of two matrices representing the desired sparsity pattern of the distributed controller and distributed observer. A limited overall performance can also be guaranteed by the proposed distributed controller which is parameterized by two scalars. To illustrate the effectiveness of the algorithm, a numerical example is provided.

Water Evaporation-Induced Self-Assembly of Hierarchical CuO/MnO2 Composite Nanospheres and their Applications in Lithium-Ion Batteries

Chunyan Zeng,Chen Gao,Li Yuan,Tao Liang,Ruisong Yang,Wei Zhang,Song Nie 성균관대학교(자연과학캠퍼스) 성균나노과학기술원 2017 NANO Vol.12 No.2

In this paper, hierarchical CuO/MnO2 composite hollow nanospheres have been successfully fabricated by water evaporation-induced self-assembly through a redox transformation reaction between Cu2O nanospheres and KMnO4 solution at 120℃ for 6 h, followed by removing the residual Cu2O cores with ammonia hydroxide solution. The outstanding feature of this method is that the reaction system is in a dynamic environment due to the evaporation of the solvent water, which benefits the self-assembly of nanostructures to form hierarchical structures. Both Kirkendall effect and Ostwald ripening mechanism are suggested to be responsible for the formation of the hierarchical CuO/MnO2 nanocomposites according to the characterization results. The electrochemical properties of the products were studied, and the results show that the hierarchical CuO/MnO2 hollow nanospheres exhibit high capacity and good rate performance (a stable capacity of about 480 mAh g-1 after 80 cycles of variable charging rate), which is probably attributed to the hierarchical hollow nanostructures.

Long Noncoding RNA Expression Profiling During the Neuronal Differentiation of Glial Precursor Cells from Rat Dorsal Root Ganglia

Yunfei Dai,Wei Ma,Tong Zhang,Jinwei Yang,Chenghao Zang,Kuangpin Liu,Xianbin Wang,Jiawei Wang,Zhen Wu,Xingkui Zhang,Chunyan Li,Junjun Li,Xiangpeng Wang,Jianhui Guo,Liyan Li 한국생물공학회 2020 Biotechnology and Bioprocess Engineering Vol.25 No.3

Long noncoding RNAs (lncRNAs) play important roles in the process of cell fate determination. However, their function and expression profiles have not yet been systematically investigated during the transdifferentiation of glial precursor cells derived from dorsal root ganglia (DRG) in the peripheral nervous system. Our results demonstrated significant differences in gene architecture and expression among the three transcript types (lncRNA, mRNA, and TUCP). Distinct differences in transcript length, exon number, and ORF length were identified between lncRNAs and mRNAs after comparative analysis of their structure and sequence conservation. We found that the upregulated lncRNAs outnumbered the downregulated lncRNAs in glial precursor cells cultured with proBDNF antiserum compared with the levels in glial precursor cells cultured without proBDNF antiserum. By a series of GO and KEGG analyses, we found that the effects of some lncRNAs on their target genes in cis were related to nerve growth factor-induced cell cycle, cell phenotype change, and neuronal differentiation. The qRT-PCR verification results of lncRNAs ENSRNOT00000091991, ENSRNOT00000087717, and LNC_000429 were mostly consistent with the sequencing results. The candidate lncRNAs may be associated with the neuronal transdifferentiation of glial precursor cells. Our study provides the first evidence for a remarkably diverse pattern of lncRNA expression during neuronal differentiation of glial precursor cells from rat DRG, and also provides a resource for lncRNA studies in the field of cell differentiation.

Extractive Biodegradation of Diphenyl Ethers in a Cloud Point System: Pollutant Bioavailability Enhancement and Surfactant Recycling

Tao Pan,Chunyan Liu,Qiao Xin,MEI-YING XU,Yangwu Deng,Wei Dong,Shuijing Yu 한국생물공학회 2017 Biotechnology and Bioprocess Engineering Vol.22 No.5

The biodegradation of diphenyl ethers (DEs) in the environment is limited by their high hydrophobicity. The enhancement of DE bioavailability by a cloud point system (CPS) was investigated in this study. Three CPSs (i.e., Triton X-114, Triton X-114 + Triton X-45, and Brij30 + TMN-3) were tested to promote DE biodegradation. Biocompatibility tests showed that the biodegradation of DE and 4-bromodiphenyl ether (4-BDE) was inhibited by TX-114, unaffected by TX-114 + TX-45, and promoted by Brij30 + TMN-3 over 48 h of cultivation with Cupriavidus basilensis and 4% (w/v) nonionic surfactants. Further optimization with 2% (w/v) Brij30 + TMN-3 yielded residual DE and 4-BDE quantities of 143 and 154 mg/L, respectively, lower than quantities in the control. During degradation, DE content did not decrease in the dilute phase, but sharply decreased in the coacervate phase, indicating that the DEs gradually diffused and transferred from the coacervate phase to the dilute phase for degradation by microbial cells. This behavior also enhanced the bioavailability of DEs in the CPS. By removing the cellrich dilute phase and adding fresh degradation medium and DE to the coacervate phase, surfactants were successfully recovered and reused twice without affecting DE biodegradation. Results demonstrated that a CPS with 2% (w/v) Brij30 + TMN-3 not only enhanced the bioavailability of DEs, but also decreased the treatment cost through surfactant recycling, which is beneficial for large-scale applications.

Optimal Control and Stabilization for Discrete-time Markov Jump Systems With Indefinite Weight Costs and Multi-channel Multiplicative Noises

Song Zhang,Chunyan Han,Wei Wang 제어·로봇·시스템학회 2023 International Journal of Control, Automation, and Vol.21 No.7

This paper is concerned with the optimal control and stabilization problems for discrete-time Markov jump systems with multi-channel multiplicative noises. The weight matrices in the cost function can be indefinite. The necessary and sufficient existence conditions for the optimal controller in the finite horizon is proposed explicitly in terms of generalized difference Riccati equations. Further, via the finite horizon optimal cost, we define a Lyapunov function to reduce the indefinite stabilization problem to a definite one. And in the infinite horizon, the necessary and sufficient condition to stabilize the Markov jump linear system in the mean-square sense is presented. Moreover, we prove that the Markov jump linear system is mean-square stabilizable if and only if the generalized coupled algebraic Riccati equation has a solution. Simultaneously, this solution is the maximal solution to the generalized coupled algebraic Riccati equations.