식이지방의 종류가 백쥐 상피 세포의 Protein Kinase C 활성에 미치는 영향

최면,김종대,주진순 ( Myeon Choe,Jong Dai Kim,Jin Soon Ju ) 생화학분자생물학회 1991 BMB Reports Vol.24 No.4

To determine the effect of dietary fats on Protein Kinase C(PKC) activity in mouse epidermal cells, female Balb/c mice (4 weeks of age) were placed on one of the 15% (w/w) of corn oil, sesame oil or beef tallow supplemented diets for at least 4 weeks. Protein kinase C was assayed by the modified procedure of Wise et al., and the activities among the groups were compared to that of corn oil group as the control. An apparent decrease of PKC activity in both cytosolic and membrane-associated fractions was observed from the animal fed sesame oil diet when compare with the animal fed corn oil diet. However, PKC activity of beef tallow fed group was increased slightly. In summary, dietary fats may contribute to mechanism of enhanced tumor promotion by changing PKC activity in mouse skin model.

식이섬유가 무기질의 생체이용에 미치는 영향

최면,Choe, Myeon 한국식품위생안전성학회 1992 한국식품위생안전성학회지 Vol.7 No.4

Among the nutrients of biological importance, minerals are of particular interest in human nutrition because the range of adequate intake is so narrow. As the results of a series of interaction experiments between dietary fiber sources and minerals, there are many inconsistencies in the experimental data regarding the effect of dietary fibers on mineral bioavailability. the mechanism by which dietary fiber might influence mineral absorption is related to its physicochemical properties. These properties involve the ability of dietary fiber to (1) act as a weak cation exchanger, (2) decrease transit time, (3) dilute mineral concentration by increasing fecal bulk and (4) resist digestion in the large bowel. Regardless of the large number of human and animal studies available, a carful review of these publications dose not provide the answer as to whether the adverse effect of dietary fibers on mineral absorption is the fiber itself or some associated dietary factors( e.g. phytate, oxalate, ascorbate, citrate and protein, mineral-mineral interaction, etc) that are responsible for this action. As a result of the complexity of interaction that may take place between minerals. dietary fiber, and other component of food ; it becomes very difficult to blame fiber alone as a negative factor of mineral nutrition. We absolutely need more research with advanced tools rather than metabolic balance study.

식이지방의 종류가 백쥐 상피세포의 Protein Kinase C 활성에 미치는 영향

최면,김종대,주진순,Choe, Myeon,Kim, Jong-Dai,Ju, Jin-Soon 생화학분자생물학회 1991 한국생화학회지 Vol.24 No.4

식이지방의 종류가 Protein Kinase C(PKC) 활성에 미치는 영향을 고찰하기 위하여 Balb/c 백서를 3군으로 나누어 옥수수유, 우지, 참깨유를 식이지방원으로 15%(w/w)씩 4주간 투여한 후 상피세포를 분리하여 Wise 등의 방법으로 PKC 활성을 측정하였다. 참깨유로 사육된 백서의 상피세포를 옥수수유로 사육된 경우와 비교할 때, cytosolic 및 membrane-associated PKC 활성은 모두가 현저히 감소하는 것이 관찰되었으며 유의적은 아니지만 우지로 사육된 백서 상피세포는 반대의 경향을 보여 증가하는 경향을 고찰하였다. PKC 활성이 cell differentiation이나 proliferation 과정에 접지적 역할을 한다는 사실과 membrane-associated PKC가 발암물질인 TPA와 같은 phorbol esters의 수용체로서의 기능을 고려할 때 본 실험결과는 식이지방의 종류가 백쥐 상피 세포의 PKC활성을 변화시킴으로서 발암과정의 저해나 촉진을 가져올지도 모른다는 것을 암시하고자 한다. To determine the effect of dietary fats on Protein Kinase C(PKC) activity in mouse epidermal cells, female Balb/c mice (4 weeks of age) were placed on one of the 15% (w/w) of corn oil, sesame oil or beef tallow supplemented diets for at least 4 weeks. Protein kinase C was assayed by the modified procedure of Wise et al., and the activities among the groups were compared to that of corn oil group as the control. An apparent decrease of PKC activity in both cytosolic and membrane-associated fractions was observed from the animal fed sesame oil diet when compare with the animal fed corn oil diet. However, PKC activity of beef tallow fed group was increased slightly. In summary, dietary fats may contribute to mechanism of enhanced tumor promotion by changing PKC activity in mouse skin model.

고혈압 환자 가족과 정상인에 있어서 혈압과 Na, K 섭취간의 상관관계

최면(Myeon Choe),김종대(Jong-Dai Kim),김성실(Sung-Sil Kim) 한국식품영양과학회 1996 한국식품영양과학회지 Vol.25 No.6

고혈압은 한국인에 있어서 발병율이 높은 성인병이며 또한 식이 중 Na 섭취와 관련이 있는 질병으로 알려져 있는바, 본 연구는 건강한 구성원만을 가진 정상인군과 고혈압 환자가 있는 가족의 구성원을 대상으로(환자 자신은 제외) 이들이 섭취하는 음식물, 소변 중의 Na 및 K과 혈압을 측정함으로서 이들 항목이 고혈압과 어떤 상관관계가 있는지를 알아보고자 하였다. 고혈압 환자의 수축기 혈압(126±18.0㎜Hg)과 이완기혈압 (77.6±14.6㎜Hg)은 정상인 가족의 수축기 혈압(119.3±17.2㎜Hg)과 이완기 혈압(71.6±12.5㎜Hg) 보다 유의적으로 높은 수치를 보였다. 수축기 혈압과 조사 항목간의 상관관계는 다음과 같다. 정상인군에서는 나이, 체중, 된장내 Na, 된장내 Na는 양의 상관관계를 보였고, 고추장내 K, 된장내 K, 고기반찬류내 K 등과 부의 상관관계를 나타냈다. 고혈압 환자 가족군에서는 나이, 체중, 소금 섭취량, 된장내 Na 등과 양의 상관관계를, 소변내 K 배설량과 부의 상관관계를 보였다. 이완기 혈압과 기타 조사항목간의 상관관계는 다음과 같다. 정상인군에서는 나이, 체중, 소금 섭취량 등과 양의 상관관계를, 고추장내 K, 된장내 K등과 부의 상관관계를 보였으며 고혈압 환자 가족군에서는 나이, 체중, 소금 섭취량 등과 양의 상관관계를, 고추장내 Na, 된장내 Na, 소변내 K 배설량 등과 부의 상관관계를 나타냈다. Purpose of this study was to investigate correlation between blood pressure(systolic and diastolic) and dietary Na, K intake pattern in the family members of normal and hypertension disease patients, besides patients themselves. Mean values of systolic(126.6±18.0 vs 119.3±17.2mmHg) and diastolic (77.6±14.6 vs 71.6±12.5mmHg) blood pressure in the family members of hypertension patients were significantly higher than those of normal subjects. Systolic blood pressure in normal subject group was positively correlated with age, body weight, sodium in soybean paste, and was negatively correlated with potassium in hotpepper paste, soybean paste and meats. Systolic blood pressure in the family member of hypertension patient was positively correlated with age, body weight, sodium in soybean paste, and table salt intake, but urinary potassium excretion was negatively correlated. Diastolic blood pressure was positively correlated with age, body weight, and table salt intake, and was negatively correlated with potassium in hotpepper paste and soybean paste in normal subjects groups. In the family members of hypertension patients group, diastolic blood pressure was positively correlated with age, body weight, and table salt intake, and was negatively correlated with sodium in hotpepper paste and soybean paste and urinary potassium excretion. In total, tendency of negative correlation between Na intake pattern and systolic blood pressure in normal subjects was much obvious than that in the family members of hypertension patients. Urinary potassium excretion in the family members of hypertension patients was also negatively correlated with both systolic and diastolic blood pressure.

식이 지방과 암

최면(Myeon Choe) 한국식품영양과학회 1991 한국식품영양과학회지 Vol.20 No.5

Evidence from recent studies in several laboratories indicates a relationship between type or level of fat in the diet and occurance of tumor at specific sites. The essential fatty acids in fat and degree of their unsaturation are important to determine the influence of a dietary fats on carcinogenesis. Alteration of dietary fat can also change carcinogenesis of cell in several tissues. Dietary fats appear to be important in both initiation and promotion stages of carcinogenesis. Several possible mechanisms have been investigated how dietary fat could affect to carcinogenesis at cellular level. One potential mechanism of dietary fat on carcinogenesis is through modulation of protein kinase C activity in the cell.

Polydextrose와 Hydrolysed Guar Gum이 지방량을 달리한 식이를 섭취한 정상백서의 지질대사에 미치는 영향

최면(Choe Myeon),김종대(Kim Jong Dai),주진순(Ju Jin Soon) 韓國營養學會 1992 Journal of Nutrition and Health Vol.25 No.3

뇌졸중 환자 가족의 음주, 흡연 및 가족병력에 관한 연구

최면(Myeon Choe),김종대(Jong-Dai Kim),김성실(Sung-Sil Kim) 한국식품영양과학회 1996 한국식품영양과학회지 Vol.25 No.6

뇌졸중은 한국인에 있어서 발병율이 높은 성인병이며 또한 음주, 흡연과 관련이 있는 질병으로 알려져 있는바, 건강한 구성원만을 가진 정상인군과 뇌졸중 환자가 있는 가족의 구성원을 대상으로(환자 자신은 제외) 음주, 흡연정도, 수입과 가족병력을 조사하여 상관관계를 규명하고자 하였다. 뇌졸중 환자 가족의 평균 음주량이 정상인 가족 보다 높게 나타났는데 이는 가족 구성원 중 30대, 40대, 50대 남자의 음주량이 높은 것에 기인하는 것으로 나타났다. 뇌졸중 환자 가족의 흡연량이 정상인 보다 높았는데 이는 주로 뇌졸중 환자 가족 중 40대와 50대 남자의 높은 흡연량에 기인하는 것으로 나타났다. 가구당 월 평균 수입은 정상인 세대가 뇌졸중 환자 가족에 비해 유의적으로 높았다(정상인 가족: 673,000±187,000원, 뇌졸중 환자 가족: 483,000±213,000 원). 뇌졸중 환자 가족에서 역시 뇌졸중 병력이 많은 것은 유전적 소인과 더불어 음주, 흡연과 상당한 관련이 있을 것으로 사료된다. This project was to investigate cerebrovascular disease incidence relation to alcohol consumption, cigarette smoking and family disease history in the family members of normal and cerebrovascular disease patients, besides patients themselves. Alcohol consumption in the family members of cerebrovascular disease patients was significantly higher, especially in male with thirties, forties and fifties, than those of normal. Mean of daily cigarette smoking in the family members of cerebrovascular disease patients was found to be significantly higher, especially in males with forties and fifties, than those of normal families. Mean of monthly income of normal subjects was significantly higher than those of cerebrovascular disease families(normal: 673,000±187,000 won, cerebrovascular: 483,000±213,000 won). A degree to be attacked with cerebrovascular disease was very higher in the family members of cerebrovascular disease patients than normal family. Such cases were much higher in father side than in mother side.

지방질원으로서 어유가 백서 상피세포의 인지질 조성 및 Protein Kinase C 활성에 미치는 영향

최면(Myeon Choe) 韓國營養學會 1994 Journal of Nutrition and Health Vol.27 No.5

한약재의 물 추출물이 당대사 관련 효소와 항산화 활성에 관한 연구

최면(Myeon Choe),김대중(Dae-Jung Kim),이현주(Hyeon-Ju Lee),유진균(Jin-Kyoun You),서동주(Dong-Joo Seo),이준희(Joon-Hee Lee),정미자(Mi Ja Chung) 한국식품영양과학회 2008 한국식품영양과학회지 Vol.37 No.5

본 연구에서는 제2형 당뇨병를 가진 GK 흰쥐(Goto-Kakizaki) 간에서 추출한 cytosol과 심장에서 추출한 mitochondria를 이용한 모델계에서 당대사 관련 효소인 glucokinase glucokinase(GCK), pyruvate dehydrogenase(PDH), acetyl-CoA carboxylase(ACC) 및 glucosidase 활성에 대한 한약재의 물 추출물의 항당뇨 효과를 연구하였다. 그리고 역시 그들의 물 추출물의 free radical 소거활성을 DPPH 방법으로 알아보았다. Free radical 소거활성은 산수유(CF), 목단피(MDB), 천화분(CHB) 그리고 산약(SY)의 물 추출물이 강했고 반면에 백목령(BBR), 숙지황(SGH)과 택사(TS)는 낮은 소거작용을 나타내었다. 간 cytosol의 GCK 활성은 산수유(CF)와 천화분(CHB)에서 다른 추출물보다 더 강했다. 심장 미토콘드리아의 PDH 활성은 택사(TS)를 제외하고 모든 추출물에서 대조군과 비교하여 높았다. 간 cytosol의 ACC 활성은 대조군보다 산수유(CF), 천화분(CHB), 숙지황(SGH), 택사(TS) 그리고 산약(SY) 추출물에서 높았다. 산수유(CF), 백복령(BBR) 및 목단피(MDB) 추출물은 α-glucosidase 활성 감소를 유도했다. 따라서 모든 추출물은 혈당 상승을 억제할 수 있는 항당뇨 기능성식품이나 약품 개발을 위한 기능성 천연 소재로 이용될 수 있을 가능성을 제시하였다. The anti-diabetic effects of water extracts (WE) from medicinal herbs on hepatic glucose-regulating enzymes, such as glucokinase (GCK), pyruvate dehydrogenase (PDH), acetyl-CoA carboxylase (ACC) and α-glucosidase, were studied using the cytosol fraction in liver and mitochondia fraction in heart of a type Ⅱ diabetic animal (GK rat, Goto-Kakizaki). The free radical scavenging activity of water extracts by DPPH method was also tested. We found that free radical scavenging activity was strong in Corni fructu (CF), Mokdan Bark (MDB), Chenhwabon (CHB) and Sanyack (SY), while that of Backbocreng (BBR), Shuckgihwang (SGH) and Taecsa (TS) was lower. For GCK activity in cytosol of liver, CF and CHB had a more effective activity than other extracts. PDH activity in mitochondria fraction of heart was higher in all of the extracts, expect for the TS extract, than in the control. ACC activity in cytosol fraction of liver was significantly higher in the CF, CHB, SGH, TS and SY extracts than in the control. CF, BBR and MDB led to a decrease in the α-glucosidase activity. Therefore, these results suggest that all of the extracts may be used as functional material in the development as anti-diabetic functional food and medicine.

고콜레스테롤혈증 유발 가토를 이용한 우리밀의 LDL 산화 및 동맥경화 예방 효과

최최면(Myeon Choe),김현숙(Hyun-Sook Kim) 한국식품영양과학회 2002 한국식품영양과학회지 Vol.31 No.1

우리밀과 수입밀의 PBS 추출물의 LDL 산화 억제 효과를 비교하였고, 고콜레스테롤혈증 유발 가토의 조직중 지질과 산화 및 대동맥의 조직 변화를 측정함으로써 우리밀이 동맥경화의 진행 양상 변화에 어떠한 효과가 있는지 검토하였다. 밒 추출물이 LDL 산화 억제 효과는 우리밀과 수입밀이 각각 62.5%, 52.8%로 나타나 우리밀 추출물의 LDL 산화 억제 효과가 수입밀에 비해 약간 높았다. 고콜레스테톨혈증 유발 가토의 간장중 TBARS 함량은 수입밀보다 우리밀 섭취시 낮게 나타나 우리밀이 지질 과산화를 억제하는데 효과가 있는 것으로 나타났지만, 신장에서의 억제 효능은 비슷한 것으로 나타났다. 대동맥의 석회 침착 발생율은 대조군에 비해 낮았지만 우리밀과 수입밀의 섭취로 인한 뚜렷한 차이는 입증되지 않았다. 이상의 결과로 우리밀 추출물의 LDL 산화 억제 효과가 입증되었으며, 우리밀 섭취가 콜레스테롤 섭취로 인한 간장중 지질 과산화적 손상을 완화시키는데 기여함을 알 수 있었지만 우리밀과 수입밀 섭취로 인한 동맥경화 예방효과에 대한 뚜렷한 차이는 나타나지 않았다. Numerous studies have suggested the involvement of oxidative processes in the pathogenesis of atherosclerosis and especially of oxidized low density lipoproteins (LDL). We studied the effect of Korean wheat on the oxidizability of LDL and the development of experimental atherosclerosis in rabbits on cholesterol diet. For the approach of the aim, antioxidative activity of wheat extracts against oxidation of LDL was investigated. The antiatherogenic effect of wheat was studied against Newzealand white (NZW) rabbits fed containing the wheat powder for 12 weeks. PBS extracts of Korean and imported wheats decreased LDL oxidation induced by copper ion by 62.3%, 52.6% respectively in comparison with control. Liver thiobarbituric acid reactive substance (TBARS) levels of rabbits were significantly lowered in Korean wheat fed group (0.397±0.029 nmol MDA/mg protein, p<0.05) compared to control (0.496±0.021 nmol MDA/mg protein) and imported wheat group (0.478± 0.019 nmol MDA/mg protein). TBARS levels of kidney showed no significant difference among treatment groups. The aorta of cholesterol-fed rabbits showed typical atherosclerotic lesions when compared with the controls, but the microscopic differences between groups was not clear. The present study suggests that Korean wheat may have higher antioxidative capacity than imported wheat and have more beneficial effects for the treatment of disease by inhibiting the oxidation of LDL.