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전사수준에서 탄소원에 따른 효모 glucoamylase의 발현
김관천,진종언 광주보건대학 1996 論文集 Vol.21 No.-
Northern hybridization of putativa gene fragments of glucoamylase from Lipomyces kononenchoae or Candida tsukubaensis glucoamylase with poly(A^(+))RNA extracted from its cells grown in several carbon sources was carried out. The synthesis of L. kononenchoae glucoamyase was several repressed by glucose while that of C. tsukubaensis glucoamylase was better in glucose than in starch. This result was consistent with that of enzyme activity.
Chin, Jong-Eon,Sun, Heung-Suk,Lee, Kwang-Jae,Choi, Tae-Jin,Ko, Yoo-Seung,Sohn, Hyun-Jung,Kim, Jeong-Joong,Jeon, Byung-Hoon,Blaise Lee, Hwang-Hee Kyung Hee Oriental Medicine Research Center 2000 International journal of oriental medicine Vol.1 No.2
Melanin is specifically produced in melanocytes. The pathway for melanin biosynthesis is mainly controlled by tyrosinase. To estimate the inhibitory effect of melanin biosynthesis from 31 medicinal plants extracts, we tested the inhibitory effects of the tyrosinase promoter on B16 mouse melanoma cells. The result of this study demonstrated that Mori Radicis Cortex and Castena Fractus extracts only in tested medicinal plant extracts have high inhibitory effects on tyrosinase promoters with very low cytotoxicity on B16 mouse melanoma cells. Therefore, extracts of Mori Radicis Cortex and Castena Fractus were evaluated as very effective negative regulators of tyrosinase gene expression.
진종언(Jong-Eon Chin),조남철(Nam-Chul Cho),김관천(Kwan-Chun Kim) 대한환경위생공학회 2006 대한환경위생공학회지 Vol.21 No.3
To estimate the inhibitory effect of Rubus coreanum extract on melanin biosynthesis, we tested its inhibitory effects on tyrosinase promoter in B16 mouse melanoma cells. Rubus coreanum extract by methanol had inhibitory effect approximately 10% on tyrosinase promoter at 100 ㎍/mL. In the MTT assay, the same extract exhibited very low cytotoxicity under 100 ㎍/mL. The fractions of methylene chloride, butyl alcohol and water did not showed the inhibitory effect on tyrosinase promoter, but the fraction of ethyl acetate exhibited inhibitory effect approximately 11% at 100 ㎍/mL.
수종의 버섯 추출물이 티로시나아제 프로모터에 미치는 효과
진종언(Jong-Eon Chin),이혜성(Hye-Sung Lee),김관천(Kwan-Chun Kim) 대한환경위생공학회 2006 대한환경위생공학회지 Vol.21 No.3
To estimate the inhibitory effect of some mushroom extract on melanin biosynthesis, we tested its inhibitory effects of five mushroom extracts on tyrosinase promoter in B16 mouse melanoma cells. In five mushroom extracts, Cordyceps militaris and Poria cocos exhibited low repression effect on tyrosinase promoter. However, Ganoderma lucidum, Paecilomyces japonicus, Phellinus linteus showed high repression effect. Especially, Paecilomyces japonicus and Phellinus linteus extracts had very higher repression effect approximately 81~83% at 100 ㎍/mL. In the MTT assay, Paecilomyces japonicus and Phellinus linteus extracts exhibited high cytotoxicity. Therefore, repression effect of the extracts were closely connected with cytotoxicity.
대두(Glycine maxim) 추출물이 티로시나아제 프로모터 활성에 미치는 효과
진종언 ( Jong Eon Chin ),김관천 ( Kwan Chun Kim ) 한국식품영양학회 2008 韓國食品營養學會誌 Vol.21 No.3
The methanolic extract of Glycine maxim increased the expression of the promoter in B16 mouse melanoma cells harboring a tyrosinase promoter. Extract concentrations of 10 μg/㎖ and 100 μg/㎖ resulted in tyrosinase promoter expression rates of approximately 113% and 184%, respectively, as compared to the control. The fraction layers consisting of butyl alcohol and methylene chloride improved expression effects on the tyrosinase promoter. In particular, the butyl alcohol fraction evidenced a high expression rate at 100 μg/㎖. In the MTT assay, the methanolic extract did not evidence cytotoxicity at concentrations under 500 μg/㎖. Therefore, the results observed with the extract of Glycine maxim showed that the substance exerted a positive effect on the tyrosinase promoter.
어성초(Houttuynia cordata) 추출물이 Tyrosinase 유전자 발현에 미치는 효과
진종언(Jong-Eon Chin),조남철(Nam-Chul Cho) 한국식품영양과학회 2005 한국식품영양과학회지 Vol.34 No.8
멜라닌 색소 생성에 관여하는 tyrosinase 유전자의 발현을 억제하는 물질을 탐색하고자 tyrosinase 프로모터를 지닌 B16 mouse melanoma cell에 어성초 메탄올 추출물을 처리한 결과 메탄올 추출물은 10 μg/mL와 100 μg/mL의 농도에서 대조군에 비해서 약 13%와 45%의 억제효과를 나타냈으며, 세포생존율은 1 μg/mL, 10 μg/mL, 100 μg/mL의 농도에서 약 104%, 101%, 74%로서 세포독성이 낮게 나타났다. Butyl alcohol과 물 분획물은 tyrosinase 유전자의 발현을 억제하는 효과가 없었지만 methylene chloride 분획물은 tyrosinase 유전자의 발현을 억제하는 효과를 나타내었으며, 특히 methylene chloride 분획물은 10 μg/mL와 100 μg/mL의 농도에서 각각 약 82%와 48%의 발현율을 나타냄으로써 대조군에 비해 tyrosinase 유전자의 발현을 크게 억제하였다. The methanol extract of Houttuynia cordata repressed the expression of tyrosinase gene of B16 mouse melanoma cell containing tyrosinase promoter. The extract repressed expression of tyrosinase gene by about 13% and 45% at 10 μg/mL and 100 μg/mL, respectively. In the MTT assay, the same extract exhibited very low cytotoxicity at 1 μg/mL, 10 μg/mL, and 100 μg/mL, respectively. The fractions of ethyl acetate, butyl alcohol, and water did not showed the repressive effect on the expression of tyrosinase gene, but methylene chloride fraction layer repressed highly at 10 μg/mL and 100 μg/mL.
진종언(Jong-Eon Chin),김관천(Kwan-Chun Kim) 대한환경위생공학회 2005 대한환경위생공학회지 Vol.20 No.3
Chestnut bark extract by methanol repressed the expression of tyrosinase gene of B16 mouse melanoma cell containing tyrosinase promoter. 10 ㎍/㎖, 100 ㎍/㎖, 1 ㎎/㎖ of the extract repressed expression of tyrosinase gene about 38%, 47%, and 78%, respectively, compared with control. In the MTT assay, the same extract exhibited very low cytotoxicity at 1 ㎍/㎖, 10 ㎍/㎖, 100 ㎍/㎖, and 1 ㎎/㎖, respectively. The fractions of Methylene chloride and ethyl acetate did not showed the repressive effect on the expression of tyrosinase gene, but the fraction of butyl alcohol repressed highly at 10 ㎍/㎖ and 100 ㎍/㎖.
Bacillus sp. DSNC 101 이 생산하는 α-L-Arabinofuranosidase의 정제 및 특성
조남철,진종언 한국식품영양학회 2001 韓國食品營養學會誌 Vol.14 No.1
Bacillus sp. DSNC 101의 배양 상징액으로부터 α-L-arabinofuranosidase를 정제하여 그 특성을 조사하였다. 정제된 효소의 분자량을 SDS-PAGE를 이용하여 측정한 결과 56kDa로 나타났으며 효소 활성도에 대한 최적 온도와 최적 pH는 각각 55℃와 7.0이었다. p-nitrophenyl-alpha-L-arabinofuranoside에 대한 Km 값과 Vmax는 1.0mM과 113.6U/㎎이었으며 Hg^2+과 Cu^2+의 첨가는 효소 반응을 완전히 저해하였다. Ba-cillus sp. DSNC 101은 탄소원으로 볏짚을 사용할 때 α-L-arabinofuranosidase를 생산하였으나 xylan이나 glucose, Cellulose를 탄소원으로 배양할 때는 생산되지 않았다. α-L-Arabinofuranosidase was purified from the culture supernatant of Bacillus sp. DSNC 101. The enzyme had a molecular weight of 56 kDa. Optimum temperature and pH for α-L-arabinofuranosidase activity were 55℃ and 7.0 respectively. The Michaelis constant(Km) and maximal reaction velocity(Vmax) for p-nitrophenyl-α-L-arabinofuranoside were 1.0 mM and 113.6 U/㎎ protein, respectively. α-L-Arabinofuranosidase was completely inhibited by HgCl_2 and CuSO_4. The enzyme was specific for the α-linked arabinoside in the furanoside configuration. The enzyme was produced during growth on agricultural residue such as rice straw, but not during growth on spelt xylan, glucose or cellobiose.
정향(Clove) 추출물이 티로시나아제 유전자의 발현에 미치는 효과
진종언,이정용,김관천,Chin, Jong-Eon,Lee, Jeong-Yong,Kim, Kwan-Chun 대한환경위생공학회 2007 대한환경위생공학회지 Vol.22 No.1
Clove extract by methanol increased expression of the tyrosinase gene on B16 mouse melanoma cells containing tyrosinase promoter. $10{\mu}g/mL$ and $100{\mu}g/mL$ of the extract showed expression rate of the tyrosinase gene about 138% and 245%, respectively, compared with control. At $500{\mu}g/mL$, expression rate of the extract was impossible to measurement by high cytotoxicity. The solvent fraction of methylene chloride also exhibited highly expression rate as methanol extract. However, the solvent fractions of butyl alcohol and water showed repressive effect on expression of tyrosinase gene at $500{\mu}g/mL$. In MTT assay, cell survival rate of the extract exhibited similar to expression rate of tyrosinase gene. That is, $10{\mu}g/mL$ and $100{\mu}g/mL$ of the extract showed the cell survival rate about 128% and 187%, respectively.