Blocking of Cell Proliferation, Cytokines Production and Genes Expression Following Administration of Cordyceps Sinensis in the Bronchoalveolar Lavage Fluids Cells

Kuo, Yuh-Chi,Wu, Chia-Lin,Tasi, Wei-Jern,Wang, Jir-Yenn,Chang, Shi-Chung,Lin, Ching-Yuang,Shiao, Ming-Shi 경희대학교 동서의학연구소 1999 INTERNATIONAL SYMPOSIUM ON EAST-WEST MEDICINE Vol.1999 No.1

Yuh-Chi Kuo¹,Chia-Lin Wn¹,Wei-jern Tasi¹,Jir-Yenn Wang²,Shi-Chung Chang₃,Ching-Yung Lin⁴,and Ming-Shi Shiao⁴¹National Research Institute of Chinese Medicine, ²Department of Biology,Fu Jen University, ³Department of Chest, ⁴Department of Pediatrics, □De[art,amt of Medical Research and Education. Taipei Veterans General Hospital, Taipei, Taiwan Yuh-Chi Kuo¹,Chia-Lin Wn¹,Wei-jern Tasi¹,Jir-Yenn Wang²,Shi-Chung Chang²,Ching-Yung Lin³,and Ming-Shi Shiao⁴.¹National Research Institute of Chinese Medicine, ²Department of Biology,Fu Jen University, ³Department of Chest, ⁴Department of Pediatrics, □De[art,amt of Medical Research and Education. Taipei Veterans General Hospital, Taipei, Taiwan. Blocking of Cell Proliferation, Cytokines Production and Genes Expression Following Administration of Cordyceps sinensis in the Bronchoalveolar Lavage Fluids Cells. Proceedings of international Symposium on East-West Medicine, Seoul. 44-64, 1999.-Cordyceps sinensis is a major parasitic fungus on larva of Lepidoptera. It is one of the well know fungi used in traditional Chinese medicine for treatment asthma, lung and kideney diseases. Many fungi belonging to the genus Cordyceps have been demonstrated to produce natural products with various biological activities. Reported biological activities of genus cordyceps include:(a)inhibition of DNA and RNA synthesis;(b)enhancement of cell differentiation;(c)restruction of cytoskeleton;(d)inhibition of protein kinase activity;(e)antitumor activity on bladder, colon, lung carcinoma as well as fibroblastoma;(f)inhibition of the infection and revers transciptase activity of human immunodeficiency virus type 1; and(g) inhibition of methylation of nucleic acid. Thus, it is suggested that the extracts of Cordyceps sp. Contain antitumor, antiviral, and many other biological activities. In our previous studies, we proved that C. sinensis contains the antitumor and immunomodulatory agents. In the present studies, effects of C. sinensis on bronchoalveolar lavage fluids(BAL)cells were demonstrated. The crude methanolic extracts of C. sinensis were fractionated into 15 fractions by silica gel column chromatography. Effects of various fraction on BAL cells proliferation and lipopolysaccharide(LPS) stimulated interleukin-1β(IL-8),platelet activating factor(PAF), interleukin-6(IL-6), tumor necrosis factor-α(TNF-α),and interleukin-8(IL-8) production on BAL cells were determined. The result indicated that the CS-19-22 fraction suppressed BAL cells proliferation activated by LPS. The median inhibitory concentration(IC50) was 6㎍/ml. The CS-19-22 fraction also decreased IL-1β,PAF,IL-6, TNF-αand IL-8 production.The results of revers transcription-polymerase chain reaction(RT-PCR) demonstrated that the CS-19-22 fraction did not affect IL-1β,PAF,IL-6, TNF-α,and IL-8 mRNAs expression in BAL cells activated by LPS. By contrast, it inhibited the IL-10 mRNA expression but enhanced IFN-γand IL-12 mRNAs expression in activated BAL cells. Moreover, the CS-19-22 fraction blocked PAF-induced platelet aggregation. It is unlikely that cytotoxicity was involved. Because no cell deths were observable. We hypothesize that inhibitory mechanisms of CS-19-22 on BAL cells proliferation may be related to the impairments of gene expression and production of cytokines in BAL cells. The explanation of C. sinensis antiasthma function may involved the following(1)C.sinenesis contained immunomodulatory agents that stimulated IFN-γand IL-12 expression in TH1cells.(2)Enhanced secretion of IFN-γand IL-12 will inhibit the Tγcells immune responses Ex. Decreasing of IL-6 and IL-10 production.(3) The impairments of cytokines production in TH2 cells suppressed th BAL cells proliferation then more cytokines production Ex.IL-1β,PAF,IL-6,TNF-αand IL-8 in activated BAL cells were decreased. (4)The suppression of TH2cells immune responses will inhibit the growth and differentiation of B cells then IgE production will be decreased. (5)Reduced production of IgE will decreased asthma attack occurring in individuals. However, the results of the present study suggested that C. sinensis may also have acted to treat asthma in part by inhibiting BAL cells proliferation and cytokine gene expression and production. Future, experiments with treatment of animals with asthma with C. sinensis will be necessary to defined whether C. sinensis can reduce asthma injury. Plans are underway for the isolation of pure principal growth inhibitors and their mechanisms of action are subjected for further study.

Reversible Data Hiding Scheme for VQ Indices Based on Absolute Difference Trees

( Chin-chen Chang ),( Thai-son Nguyen ),( Chia-chen Lin ) 한국인터넷정보학회 2014 KSII Transactions on Internet and Information Syst Vol.8 No.7

Reversible data hiding is a technique for recovering original images without any distortion after secret data are extracted from the image. The technique continues to attract attention from many researchers. In this paper, we introduce a new reversible data hiding scheme based on the adjacent index differences of vector quantization (VQ) indices. The proposed scheme exploits the differences between two adjacent indices to embed secret data. Experimental results show that our scheme can achieve a lower compression rate than an earlier scheme by Yang and Lin. Our scheme`s average compression rate, 0.44 bpp, outperforms that of Yang and Lin`s scheme, which averages 0.53 bpp. Moreover, the embedding capacity of our scheme can rise to 1.45 bpi, which also is superior to that of Chang et al.`s scheme [35] (1.00 bpi)Yang and Lin`s scheme [27] (0.91 bpi) as well as Chang et al.`s scheme [26] (0.74 bpi).

Molecular Origins of the High-Performance Nonlinear Optical Susceptibility in a Phenolic Polyene Chromophore: Electron Density Distributions, Hydrogen Bonding, and ab Initio Calculations

Lin, Tze-Chia,Cole, Jacqueline M.,Higginbotham, Andrew P.,Edwards, Alison J.,Piltz, Ross O.,Pé,rez-Moreno, Javier,Seo, Ji-Youn,Lee, Seung-Chul,Clays, Koen,Kwon, O-Pil American Chemical Society 2013 JOURNAL OF PHYSICAL CHEMISTRY C - Vol.117 No.18

<P>The molecular and supramolecular origins of the superior nonlinear optical (NLO) properties observed in the organic phenolic triene material, OH1 (2-(3-(4-hydroxystyryl)-5,5-dimethylcyclohex-2-enylidene)malononitrile), are presented. The molecular charge-transfer distribution is topographically mapped, demonstrating that a uniformly delocalized passive electronic medium facilitates the charge-transfer between the phenolic electron donor and the cyano electron acceptors which lie at opposite ends of the molecule. Its ability to act as a “push–pull” π-conjugated molecule is quantified, relative to similar materials, by supporting empirical calculations; these include bond-length alternation and harmonic-oscillator stabilization energy (HOSE) tests. Such tests, together with frontier molecular orbital considerations, reveal that OH1 can exist readily in its aromatic (neutral) or quinoidal (charge-separated) state, thereby overcoming the “nonlinearity-thermal stability trade-off”. The HOSE calculation also reveals a correlation between the quinoidal resonance contribution to the overall structure of OH1 and the UV–vis absorption peak wavelength in the wider family of configurationally locked polyene framework materials. Solid-state tensorial coefficients of the molecular dipole, polarizability, and the first hyperpolarizability for OH1 are derived from the first-, second-, and third-order electronic moments of the experimental charge-density distribution. The overall solid-state molecular dipole moment is compared with those from gas-phase calculations, revealing that crystal field effects are very significant in OH1. The solid-state hyperpolarizability derived from this charge-density study affords good agreement with gas-phase calculations as well as optical measurements based on hyper-Rayleigh scattering (HRS) and electric-field-induced second harmonic (EFISH) generation. This lends support to the further use of charge-density studies to calculate solid-state hyperpolarizability coefficients in other organic NLO materials. Finally, this charge-density study is also employed to provide an advanced classification of hydrogen bonds in OH1, which requires more stringent criteria than those from conventional structure analysis. As a result, only the strongest OH···NC interaction is so classified as a true hydrogen bond. Indeed, it is this electrostatic interaction that influences the molecular charge transfer: the other four, weaker, nonbonded contacts nonetheless affect the crystal packing. Overall, the establishment of these structure–property relationships lays a blueprint for designing further, more NLO efficient, materials in this industrially leading organic family of compounds.</P><P><B>Graphic Abstract</B> <IMG SRC='http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/jpccck/2013/jpccck.2013.117.issue-18/jp400648q/production/images/medium/jp-2013-00648q_0007.gif'></P><P><A href='http://pubs.acs.org/doi/suppl/10.1021/jp400648q'>ACS Electronic Supporting Info</A></P>

Laser brazing molybdenum using two titanium base fillers

Lin, Chia-Chen,Lee, Cheng-Han,Shiue, Ren-Kae,Shy, Hsiou-Jeng Techno-Press 2012 Advances in materials research Vol.1 No.3

Brazing Mo using Ti and Ti-15-3 foils has been investigated in the experiment. For traditional furnace brazing, solidification shrinkage voids cannot be completely removed from the joint even the brazing temperature increased to 2013 K and 160 ${\mu}m$ thick Ti foil applied in brazing. Similar results are observed from the joint using Ti-15-3 filler. In contrast, the quality of laser brazed joint is much better than that of furnace brazed joint. A sound joint is achieved after laser brazing. Tensile strengths of 418 and 373 MPa are obtained from laser brazed joints at the power of 800W and travel speed of 5 mm/s using Ti and Ti-15-3 fillers, respectively. All laser brazed joints are fractured at the brazed zone and cleavage dominated fractures are widely observed from their fractographs. The Ti base fillers show potential in laser brazing Mo substrate.

Degradation of polyvinyl alcohol in aqueous solutions using UV/oxidant process

Chia-Chang Lin,Li-Ting Lee 한국공업화학회 2015 Journal of Industrial and Engineering Chemistry Vol.21 No.1

This study evaluates the effectiveness of the UV/S2O82 and UV/H2O2 processes in degrading polyvinylalcohol (PVA) in aqueous solutions. The effects of pH, oxidant dosage, and initial PVA concentration onthe efficiency of degradation of PVA were examined. Under acidic conditions, the efficiencies ofdegradation of PVA by the UV/S2O82 and UV/H2O2 processes exceeded those under alkaline conditions. The efficiencies of degradation of PVA by the UV/S2O82 and UV/H2O2 processes increased with oxidantdosage, but fell as the initial PVA concentration was increased. In the UV/S2O82 process, adding salts(Na2SO4, NaNO3, and NaCl) reduced the efficiency and rate of degradation of PVA. However, in the UV/H2O2 process, adding Na2SO4 and NaNO3 did not influence the degradation of PVA, whereas adding NaClweakened the degradation of PVA to a greater extent than in the UV/S2O82 process. At pH 3 with anoxidant dosage of 0.25 mM and an initial PVA concentration of 20 mg/L, the degradation efficiencies ofthe UV/S2O82 and UV/H2O2 processes, as measured after 5 min, were 100 and 58%, respectively. Thecorresponding observed degradation rate constants of the UV/S2O82 and UV/H2O2 processes were1.5509 and 0.2052 min 1, respectively. This result is attributable to the fact that SO4 was better able todegrade PVA than was OH . Therefore, the UV/S2O82 process was more effective than the UV/H2O2 process in degrading PVA.

Analysis and Design of a PFC AC–DC Converter with Electrical Isolation

Chia-Ching Lin,Lung-Sheng Yang,Ren-Jun Zheng 전력전자학회 2014 JOURNAL OF POWER ELECTRONICS Vol.14 No.5

This study presents a single-phase power factor correction AC?DC converter that operates in discontinuous conduction mode. This converter uses the pulse-width modulation technique to achieve almost unity power factor and low total harmonic distortion of input current for universal input voltage (90 Vrms to 264 Vrms) applications. The converter has a simple structure and electrical isolation. The magnetizing-inductor energy of the transformer can be recycled to the output without an additional third winding. The steady-state analysis of voltage gain and boundary operating conditions are discussed in detail. Finally, experimental results are shown to verify the performance of the proposed converter.

The Impact of Consumer Evaluation on the Cause-Related Marketing

Chia-Lin Lee 한국마케팅학회 2017 ASIA MARKETING JOURNAL Vol.19 No.1

Cause-related Marketing (CrM) has become an increasingly popular marketing approach over the past two decades. However, neither researchers nor organizations fully understand the determinants of a successful CrM partnership. This research fills thi s gap. Specifically, we employ the schema theory to explore circumstances in which the CrM alliance cannot achieve a success. We use a theoretical modeling approach to report that, when consumers’ typicality-based cognitive process is assumed, the CrM activity with the partners’ more-discrepant attribute profile cannot be evaluated favorably, but the attribute-level uncertainty about the CrM alliance is less likely to feedback to the two partners. Furthermore, we argue that, under the schema-plus-tag model, consumers may not like the CrM program with a similar attribute profile. Therefore, this CrM approach may fail. To our knowledge, we are the first to apply the schema theory to explain how a CrM alliance can achieve a success.

On H\"{o}lder-McCarthy-type inequalities with powers

Chia-Shiang Lin,조열제 대한수학회 2002 대한수학회지 Vol.39 No.3

We extend the H"{o}lder-McCarthy inequality for a positiveand an arbitrary operator, respectively. The powers of each inequality are givenand the improved Reid's inequality by Halmos is generalized. We also givethe bound of the H"{o}lder-McCarthy inequality by recursion.

RIGHT-HANDED SNEUTRINO CURVATON AND NON-GAUSSIANITY

LIN, CHIA-MIN,CHEUNG, KINGMAN World Scientific 2010 Modern Physics Letters A Vol.25 No.26

<P> In this paper, we explore the parameter space for a Right-Handed (RH) sneutrino curvaton that can generate large non-Gaussianity without assuming any particular inflation sector. The mass of the RH sneutrino is suggested from a discussion on the initial condition of the curvaton field. It is shown that a small Yukawa coupling is generally required for a successful RH sneutrino curvaton. However, the Yukawa coupling can be larger if we consider the braneworld scenario. Some general discussion about the spectral index in curvaton scenario is also provided. </P>

Developing a Virus-Binding Bacterium Expressing Mx Protein on the Bacterial Surface to Prevent Grouper Nervous Necrosis Virus Infection

( Chia-hua Lin ),( Jun-jie Chen ),( Chiu-min Cheng ) 한국미생물 · 생명공학회 2021 Journal of microbiology and biotechnology Vol.31 No.8

Grouper nervous necrosis virus (GNNV) infection causes mass grouper mortality, leading to substantial economic loss in Taiwan. Traditional methods of controlling GNNV infections involve the challenge of controlling disinfectant doses; low doses are ineffective, whereas high doses may cause environmental damage. Identifying potential methods to safely control GNNV infection to prevent viral outbreaks is essential. We engineered a virus-binding bacterium expressing a myxovirus resistance (Mx) protein on its surface for GNNV removal from phosphate-buffered saline (PBS), thus increasing the survival of grouper fin (GF-1) cells. We fused the grouper Mx protein (which recognizes and binds to the coat protein of GNNV) to the C-terminus of outer membrane lipoprotein A (lpp-Mx) and to the N-terminus of a bacterial autotransporter adhesin (Mx-AIDA); these constructs were expressed on the surfaces of Escherichia coli BL21 (BL21/lpp-Mx and BL21/Mx-AIDA). We examined bacterial surface expression capacity and GNNV binding activity through enzyme-linked immunosorbent assay; we also evaluated the GNNV removal efficacy of the bacteria and viral cytotoxicity after bacterial adsorption treatment. Although both constructs were successfully expressed, only BL21/lpp-Mx exhibited GNNV binding activity; BL21/lpp-Mx cells removed GNNV and protected GF-1 cells from GNNV infection more efficiently. Moreover, salinity affected the GNNV removal efficacy of BL21/lpp-Mx. Thus, our GNNV-binding bacterium is an efficient microparticle for removing GNNV from 10‰ brackish water and for preventing GNNV infection in groupers.