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Induction of Salivary Gland-Like Tissue by Induced Pluripotent Stem Cells In Vitro
Meng Cen,Huang Shengyuan,Cheng Taiqi,Zhang Xue,Yan Xing 한국조직공학과 재생의학회 2022 조직공학과 재생의학 Vol.19 No.2
Background: To investigate the in vitro induction of salivary gland-like tissue by ips cells in an interferon regulatory factor 6 (IRF6) overexpression and parotid conditioned medium environment. Methods: Urine-derived ips cells were isolated, identified, transfected with IRF6 and cultured in parotid conditioned medium to induce ips cells into salivary gland differentiation, morphological changes of ips cells were observed, CCK-8 was used to determine the cell proliferation efficiency and transcriptome sequencing was used to detect the expression of genes related to parotid gland formation. Results: Immunofluorescence staining showed that the isolated ips cells were positive for NANOG, SSEA4 and OCT4 and had embryonic-like stem cell characteristics; CCK-8 showed that there was no statistical difference in the proliferation efficiency between the IRF6+ induced group and the simple induced group after induction of ips cells into salivary glands. The results of transcriptome sequencing showed that there were a total of 643 differentially expressed genes, including 365 up-regulated genes and 278 down-regulated genes in the IRF6+ induced group compared to the blank control group, and the salivary gland related genes HAPLN1, CCL2, MSX2, ANXA1, CYP11A1, HES1 and LUM were all highly expressed in the IRF6+ induced group. Conclusion: IRF6 promotes salivary gland differentiation in urine-derived iPSCs, and its mechanism of promoting differentiation may be that IRF6 upregulates the expression of HAPLN1, CCL2, MSX2, ANXA1, CYP11A1, HES1 and LUM to promote epithelial differentiation.
Edaphovirga cremea gen. nov., sp. nov., isolated from the rhizospheric soil of Codonopsis clematidea
Jin-Yan Xue,Meng-Yue Zhang,Yu Zhang,Juan Cheng,Li-Cheng Liu,Ying-Ying Wu,Tian-Yuan Zhang,Yi-xuan Zhang 한국미생물학회 2019 The journal of microbiology Vol.57 No.5
A Gram-negative, facultatively anaerobic, non-motile, nonspore- forming, coccoid or rod-shaped and creamy-pigmented bacterium, designated SYP-B2100T, was isolated from the rhizospheric soil of Codonopsis clematidea in the Xinjiang Uygur Autonomous Region, China. The optimal growth occurred at 28°C, pH 5.0, in the absence of NaCl. The cells tested positive in catalase and methyl red tests but negative in oxidase, urease, gelatinase, milk coagulation, and peptonisation, H2S production, nitrate reduction, and Voges-Proskauer tests. The major isoprenoid quinone was ubiquinone-8 (Q-8). The major cellular fatty acids were C16:0 and summed feature 8. The polar lipids consisted of diphosphatidylglycerol, phosphatidylethanolamine, and phosphatidylglycerol. The 16S rRNA gene sequence of strain SYP-B2100T was the most similar to that of Rahnella inusitata DSM 30078T (96.9%) within the family Enterobacteriaceae. The genomic DNA G+C content of strain SYP-B2100T was 50.3 mol%. The combined data from the phylogenetic, morphological, physiological, biochemical, and chemotaxonomic analyses presented in this study support the conclusion that strain SYP-B2100T represents a novel species of a new genus, for which the name Edaphovirga cremea gen. nov., sp. nov. is proposed; the type strain is SYPB2100T (= CGMCC 1.5857T = DSM 105170T = KCTC 62024T).
Effects of Ohmic Area Etching on Buffer Breakdown Voltage of AlGaN/GaN HEMT
Chong Wang,Xiao-Xiao Wel,Meng-Di Zhao,Yun-Long He,Xue-Feng Zheng,Wei Mao,Xiao-Hua Ma,Jin-Cheng Zhang,Yue Hao 한국전기전자재료학회 2017 Transactions on Electrical and Electronic Material Vol.18 No.3
This study is on how ohmic area etching affects the buffer breakdown voltage of AlGaN/GaN HEMT. The surfacemorphology of the ohmic metal can be improved by whole etching on the ohmic area. The buffer breakdown voltagesof the samples with whole etching on the ohmic area were improved by the suppression of the metal spikes formedunder the ohmic contact regions during high-temperature annealing. The samples with selective etching on theohmic area were investigated for comparison. In addition, the buffer leakage currents were measured on the differentradii of the wafer, and the uniformity of the buffer leakage currents on the wafer were investigated by PL mappingmeasurement.
Chen Xiuling,Mo Fulei,Shen Chunyang,Meng Lingjun,Zou Yuxin,Xue Xiaopeng,Cheng Mozhen,Meng Fanyue,Qi Haonan,Wang Aoxue 한국원예학회 2022 Horticulture, Environment, and Biotechnology Vol.63 No.6
NAC proteins in plants respond to stress and play an important role in plant growth regulation. This regulation occurs through a variety of downstream eff ects mediated by plant hormones in response to stress. In this study, we performed a systematic genome-wide analysis of the NAC gene family in tomato based on genome version SL4.0. We identifi ed 99 SlNAC genes by abiotic stress analysis of conserved motifs and gene structure, phylogenetic analysis, cis-element analysis, chromosome localization analysis, synteny relationship and expression pattern analysis. These 99 SlNAC genes were distributed on all 12 chromosomes. Except for genes without introns, there was no signifi cant correlation between gene structures and phylogenetic relationships. Most of the cis-elements identifi ed were associated with plant hormones and environmental responses. There were 77 SlNAC genes that showed no homogeneity between tomato and wild rice, suggesting that these homologous genes arose after the diff erentiation of dicotyledonous and monocotyledonous plants. The comprehensive analysis of the SlNAC gene family provides a valuable resource for studying this gene family in tomato and a foundation for further study of the functional characteristics of these genes.
Effects of Ohmic Area Etching on Buffer Breakdown Voltage of AlGaN/GaN HEMT
Wang, Chong,Wel, Xiao-Xiao,Zhao, Meng-Di,He, Yun-Long,Zheng, Xue-Feng,Mao, Wei,Ma, Xiao-Hua,Zhang, Jin-Cheng,Hao, Yue The Korean Institute of Electrical and Electronic 2017 Transactions on Electrical and Electronic Material Vol.18 No.3
This study is on how ohmic area etching affects the buffer breakdown voltage of AlGaN/GaN HEMT. The surface morphology of the ohmic metal can be improved by whole etching on the ohmic area. The buffer breakdown voltages of the samples with whole etching on the ohmic area were improved by the suppression of the metal spikes formed under the ohmic contact regions during high-temperature annealing. The samples with selective etching on the ohmic area were investigated for comparison. In addition, the buffer leakage currents were measured on the different radii of the wafer, and the uniformity of the buffer leakage currents on the wafer were investigated by PL mapping measurement.
Association Between MDM2 SNP309 T>G and Risk of Gastric Cancer: A Meta-analysis
Tian, Xin,Tian, Ye,Ma, Ping,Sui, Cheng-Guang,Meng, Fan-Dong,Li, Yan,Fu, Li-Ye,Jiang, Tao,Wang, Yang,Ji, Fu-Jian,Fang, Xue-Dong,Jiang, You-Hong Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.3
Background: As a negative regulator of P53, MDM2 plays an important role in carcinogenesis; a polymorphism in its promoter region. SNP309 T>G, is known to increase the expression of MDM2, thus being considered related to higher susceptibility to neoplasia. However, no agreement has been achieved regarding its effects on gastric cancer. Methods: The present systematic meta-analysis was performed based on comprehensive literature search from Pubmed, Web of science and CBM databases. Results: It was suggested from 6 independent studies that the GG genotype is associated with a significantly increased risk of gastric cancer (Recessive: OR = 1.43, 95% CI = 1.08-1.91, P = 0.013), and subgroup analysis also confirmed the relationship (English publications-recessive model: OR = 1.45, 95% CI = 1.10-1.91, P = 0.009; Studies in China-recessive model: OR = 1.58, 95% CI = 1.08-2.30, P = 0.017). No publication bias was detected. Conclusion: The meta-analysis indicated a significant inverse association between GG genotype carriage and elevated risk of gastric cancer. However, more studies and detailed information are needed to fully address the topic.
Li, Bin,Pang, Yong-jie,Cheng, Yan-xue,Zhu, Xiao-meng The Society of Naval Architects of Korea 2017 International Journal of Naval Architecture and Oc Vol.9 No.4
A Collaborative Optimization (CO) methodology for ring-stiffened composite material pressure hull of underwater vehicle is proposed. Structural stability and material strength are both examined. Lamination parameters of laminated plates are introduced to improve the optimization efficiency. Approximation models are established based on the Ellipsoidal Basis Function (EBF) neural network to replace the finite element analysis in layout optimizers. On the basis of a two-level optimization, the simultaneous structure material collaborative optimization for the pressure vessel is implemented. The optimal configuration of metal liner and frames and composite material is obtained with the comprehensive consideration of structure and material performances. The weight of the composite pressure hull decreases by 30.3% after optimization and the validation is carried out. Collaborative optimization based on the lamination parameters can optimize the composite pressure hull effectively, as well as provide a solution for low efficiency and non-convergence of direct optimization with design variables.
BIN LI,Yong-jie Pang,Yan-xue Cheng,Xiao-meng Zhu 대한조선학회 2017 International Journal of Naval Architecture and Oc Vol.9 No.4
A Collaborative Optimization (CO) methodology for ring-stiffened composite material pressure hull of underwater vehicle is proposed. Structural stability and material strength are both examined. Lamination parameters of laminated plates are introduced to improve the optimization efficiency. Approximation models are established based on the Ellipsoidal Basis Function (EBF) neural network to replace the finite element analysis in layout optimizers. On the basis of a two-level optimization, the simultaneous structure material collaborative optimization for the pressure vessel is implemented. The optimal configuration of metal liner and frames and composite material is obtained with the comprehensive consideration of structure and material performances. The weight of the composite pressure hull decreases by 30.3% after optimization and the validation is carried out. Collaborative optimization based on the lamination parameters can optimize the composite pressure hull effectively, as well as provide a solution for low efficiency and non-convergence of direct optimization with design variables.
Roles of Fibroblast Growth Factor-inducible 14 in Hepatocellular Carcinoma
Li, Nan,Hu, Wen-Jun,Shi, Jie,Xue, Jie,Guo, Wei-Xing,Zhang, Yang,Guan, Dong-Xian,Liu, Shu-Peng,Cheng, Yu-Qiang,Wu, Meng-Chao,Xie, Dong,Liu, Shan-Rong,Cheng, Shu-Qun Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.6
The prognostic value of the fibroblast growth factor-inducible 14 (Fn14) expression in hepatocellular carcinoma (HCC) is unknown. Real-time PCR (RT-PCR), western blot assays and immunohistochemistry analysis were here performed in order to compare Fn14 expressios in paired liver samples of HCC and normal liver tissue. Most of the tumor tissues expressed significantly higher levels of Fn14 compared to adjacent non-tumor tissues, with Fn14High accounting for 54.6% (142/260) of all patients. The Pearson ${\chi}^2$ test indicated that Fn14 expression was closely associated with serum alpha fetal protein (AFP) (P=0.002) and tumor number (p=0.019). Univariate and multivariate analyses revealed that along with tumor diameter and portal vein tumor thrombosis (PVTT ) type, Fn14 was an independent prognostic factor for both overall survival (OS) (HR=1.398, p=0.008) and recurrence (HR=1.541, p=0.001) rates. Fn14 overexpression HCC correlated with poor surgical outcome, and this molecule may be a candidate biomarker for prognosis as well as a target for therapy.