Effect of Grifola frondosa polysaccharides (GFPS) on the improvement of clinical symptoms in letrozole-induced polycystic ovary syndrome (PCOS) rats

Chaojie Chong,Yaqi Wu,Zhewen Dong,Zixian Wang,Shumei Zhong,Haokun Chen,Qi Qi Pang,Jia-Le Song,Yanyuan Zhou 한국식품영양과학회 2021 한국식품영양과학회 학술대회발표집 Vol.2021 No.10

This study was to investigate the ameliorative effect of GFPS on clinical symptoms in letrozole(1 mg/kg)-induced PCOS rats. The female SD rats were classified into 4 groups as follows: the normal group (gavaged with saline), the PCOS group(ingested letrozole daily for 21 days). Normal group and metformin group gavaged with letrozole followed by metformin (265 mg/kg) and GFPS(600 mg/kg), respectively. All rats were weighed every 3 days until the end of the experiment. The ovaries, perirenal, abdomen and periuterine fat were removed and the relative weights (g/bw×100) were calculated. Serum levels of FSH, LH, E2, T, TG, TC, LDL-C were measured by ELISA and calculated the LH/FSH ratio. GFPS reduced follicle number and increases granulosa cell layer thickness and luteal number. GFPS also reduced the increase of body weight, ovarian and visceral fat weight in PCOS rats (P<0.01). In addition, GFPS reduced the serum levels of T, LH/FSH, TC, TG, LDL-C and LH, and elevated the serum levels of E2 and FSH in PCOS rats. In conclusion, GFPS can improve follicular development and restore body weight, ovarian weight, serum hormone levels and lipid profile in PCOS rats.

Effect of Antrodia camphorata polysaccharides on letrozole-induced polycystic ovary syndrome (PCOS) model rats

Zhiqiang Liu,Chaojie Chong,Zhewen Dong,Yaqi Wu,Wanying Li,Haokun Chen,Shumei Zhong,Junyang Liu,Qi Qi Pang,Jia-Le Song,Yanyuan Zhou 한국식품영양과학회 2021 한국식품영양과학회 학술대회발표집 Vol.2021 No.10

To investigate the effect of ACP on letrozole-induced PCOS rats. All rats were divided into normal groups, PCOS group, metformin group and ACP group. The body weight (BW) was recorded every three days, and the final body weight as well as ovarian weight were recorded. Serum levels of TC, TG, HDL-C, LDL-C and E2, T, FSH and LH were measured by ELISA assay, and the LH/FSH ratio was calculated. The results showed that ACP decreased the BW and the ratio of ovarian to BW. Compared with the normal group, the serum levels of T, LH, LH/FSH, TG, TC, LDL-C increased, and the levels of FSH, E2, HDL-C decreased in PCOS group. ACP effectively improves the serum levels of FSH, LH/FSH, TG, TC, LDL-C and other related factors. The results of H&E staining of ovarian tissues showed that the ACP group showed different degrees of improvement in the reduced number of corpus luteum, thinning of granulosa cell layer, follicular capsular dilatation and follicular atresia. ACP not only reduces BW and the ratio of ovarian to BW, but also it can elevate the level of FSH and reduce LH/FSH, TG, TC and HDL-C levels. Furthermore, ACP can protect ovarian tissue to some extent.

Effect of Phellinus linteus polysaccharides on improving polycystic ovary syndrome (PCOS) induced by letrozole in SD rats

Yaqi Wu,Chaojie Chong,Zhewen Dong,Zixian Wang,Haokun Chen,Shumei Zhong,Qi Qi Pang,Jia-Le Song,Yanyuan Zhou 한국식품영양과학회 2021 한국식품영양과학회 학술대회발표집 Vol.2021 No.10

To explore the ameliorative effect of Phellinus linteus polysaccharide(PLP) on letrozole-induced PCOS rats. A total of 32 female SD rats were randomly divided into normal group, PCOS group, metformin group and PLP group, and weighed every three days. The estrous cycle of rats was observed by vaginal smear ten days before sacrifice. The estrous cycle of PCOS rats was disordered, the ovaries showed polycystic changes and the body weight and the relative weight of the ovaries increased (P<0.05). The estrous cycle, polycystic ovary, body weight and relative weight of the ovaries in PLP group and Metformin group recovered significantly (P<0.05). The serum levels of TC, TG, LDL-C, HDL-C, FINS, T, LH, E2 and FSH were detected by ELISA kits. After treatment, the serum levels of TC, TG, LDL-C, FINS, T, LH and LH/FSH in the PLP group and Metformin group were lower than PCOS group (P<0.05). In addition, the serum levels of E2, FSH and HDL-C were increased (P<0.05) in the PLP group and Metformin group. The results showed that Phellinus linteus polysaccharides can ameliorate glucose and lipid metabolism, polycystic ovary and regulate the levels of sex hormones in PCOS rats.

Fire Performance of Continuous Steel-Concrete Composite Bridge Girders

Gang Zhang,Chaojie Song,Xuyang Li,Shuanhai He,Qiao Huang 대한토목학회 2021 KSCE Journal of Civil Engineering Vol.25 No.3

This paper presents a detailed investigation in to fire resistance along with failure mode in continuous steel-concrete composite twin I-shaped bridge girders under different localized fire through considering fire severity and fire exposure positions namely; mid-span zone in one of the two span, hogging moment zone and side-support zone. A 3-D finite element (FE) model, built utilizing the computer program ANSYS, is used to track structural fire performance in a typical two span of continuous composite twin I-shaped bridge girders (fabricated with twin I-shaped plates supporting a concrete slab) dependent on thermo-mechanical coupled analysis. The model validation is undertook through comparison of temperature and deflection response attained from a scaled composite single I-shaped girder tested exposed to ISO834 fire. The numerical analysis results show that the developed model can be favorably used to analyze the behavior and failure mode of continuous steel-concrete composite twin I-shaped bridge girders during entire range of fire exposure. Fire severity and fire exposure positions present critical influence in to the fire resistance of continuous composite bridge girders. Fire exposure prevention on hogging moment zone can significantly extend failure time of continuous girders, and further hold back progressive structural collapse. Web buckling based failure criterion can be applicable to calculate fire resistance of realistic continuous girders under simultaneous structural loading together with localized fire. Continuous composite bridge girders subjected to localized fire present highly significant local deformation response in the fire exposed bridge girder span.

A numerical method for evaluating fire performance of prestressed concrete T bridge girders

Gang Zhang,Venkatesh Kodur,Chaojie Song,Wei Hou,Shuanhai He 사단법인 한국계산역학회 2020 Computers and Concrete, An International Journal Vol.25 No.6

This paper presents a numerical method for evaluating fire performance of prestressed concrete (PC) T shaped bridge girders under combined effect of structural loading and hydrocarbon fire exposure conditions. A numerical model, developed using the computer program ANSYS, is employed to investigate fire response of PC T shaped bridge girders by taking into consideration structural inherent parameters, namely; arrangement of prestressing strands with in the girder section, thickness of concrete cover over prestressing strands, effective degree of prestress and content of prestressing strands. Then, a sequential thermo-mechanical analysis is performed to predict cross sectional temperature followed by mechanical response of T shaped bridge girders. The validity of the numerical model is established by comparing temperatures, deflections and failure time generated from fire tests. Through numerical studies, it is shown that thickness of concrete cover and arrangement of prestressing strands in girder section have significant influence on the fire resistance of PC T shaped bridge girders. Increase in effective degree of prestress in strands with triangular shaped layout and content in prestressing strands can slow down the progression of deflections in PC T shaped bridge girder towards the final stages of fire exposure, to thereby preventing sudden collapse of the girder. Rate of deflection based failure criterion governs failure in PC T shaped bridge girders under most hydrocarbon fire exposure conditions. Structural inherent parameters incorporated into sectional configuration can significantly enhance fire resistance of PC bridge girders; thus mitigating fire induced collapse of these bridge girders.

The effect of cyanidin-3,5-O-diglucoside on the improvement of dextran sodium sulfate-induced intestinal fibrosis in mice with colitis

Zixian Wang,Wanying Li,Zhewen Dong,Chaojie Chong,Yaqi Wu,Shumei Zhong,Haokun Chen,Qi Qi Pang,Jia-Le Song 한국식품영양과학회 2021 한국식품영양과학회 학술대회발표집 Vol.2021 No.10

To observe the effect of cyanidin-3,5-O-diglucoside(C3G) on intestinal fibrosis in a model of dextran sulphate sodium (DSS)-induced mice with colitis. Colitis was induced in C57BL/6 mice by addition of DSS to drinking water. Clinical (body weight, colon length and colon weight) and histologic changes were evaluated. In addition, colon levels of interleukin-1β, IL-6, IL-18, IL-17A, tumor necrosis factor(TNF)-α were detected by enzyme-linked immunosorbent assay. Relative to the DSS group, the mice in the C3G treatment group increased in body weight, colon length, and the ratio of colon weight to length was significantly lower (P<0.05). The DAI index of mice in the intervention group was lower than that in the colitis model group (P<0.05). Histological observation also found that the intervention of C3G is able to reduce the infiltration of inflammatory cells in colon tissue induced by DSS. The results of Masson staining suggest that the intervention of C3G also reduces the occurrence of intestinal fibrosis in mice with colitis. Our results suggested that C3G is an activity to improve inflammation and intestinal fibrosis in DSS colitis mice.

Sinapic acid(SA) attenuated the letrozole-induced ovarian fibrosis and modulated the abolic disturbances in polycystic ovary syndrome(PCOS) SD rat

Huan Lan,Zhewen Dong,Yaqi Wu,Chaojie Chong,Zhiqiang Liu,Wanying Li,Zixian Wang,Junyang Liu,Qi Qi Pang,Jia-Le Song,Yanyuan Zhou 한국식품영양과학회 2021 한국식품영양과학회 학술대회발표집 Vol.2021 No.10

This study investigated the antifibrotic effects of SA on ovarian fibrosis in letrozole-induced PCOS in rats. SA treatment restored letrozole-induced alterations in the body weight (BW), relative weights of the ovaries, and visceral adipose tissues. Histological observation showed that SA reduced the number of atretic follicles, cystic follicles, and fibrosis of the ovaries in PCOS rats. SA treatment also modulated the serum levels of sex hormones, which decreased the luteinizing hormone (LH), and testosterone (T) and increased the follicle-stimulating hormone (FSH) in PCOS rats. Administration of SA reduced letrozole-induced metabolic dysfunction by decreasing the serum levels of triglyceride (TG) and total cholesterol (TC) and increasing the levels of high-density lipoprotein cholesterol (HDL-C) in PCOS rats. Finally, SA treatment appeared to increase the activity of PPAR-γ, reduce the activation of the TGF-β1/Smad pathway, and reduce the collagen I, α-SMA and CTGF levels in the ovaries of the PCOS rats. Our results demonstrated that SA significantly ameliorated ovarian fibrosis and abolic disturbances in the letrozole-induced PCOS rats.

Rhamnocitrin (Rha) attenuates ovarian fibrosis in rats with letrozole-induced experimental polycystic ovary syndrome (PCOS)

Huan Lan,Zhewen Dong,Yaqi Wu,Chaojie Chong,Zhiqiang Liu,Wanying Li,Zixian Wang,Junyang Liu,Qi Qi Pang,Jia-Le Song,Yanyuan Zhou 한국식품영양과학회 2021 한국식품영양과학회 학술대회발표집 Vol.2021 No.10

In this study, we investigated the antifibrotic effects of Rha on letrozole-induced PCOS in rats. Rha treatment restored letrozole-induced alterations in the body weight (BW), relative weights of the ovaries, and relative weights of uterine and visceral adipose tissues. Histological observation showed that Rha reduced the number of atretic follicles, cystic follicles, and fibrosis of the ovaries in the PCOS rats. Administration of Rha reduced letrozole-induced metabolic dysfunction by decreasing the serum levels of TG and TC and increasing the levels of HDL-C in the PCOS rats. Rha treatment also modulated the serum levels of sex hormones, which decreased the LH, E2 and T and increased the FSH in PCOS rats. Finally, Rha treatment appeared to increase the activity of PPAR-γ, reduce the activation of the TGF-β1/Smad pathway, and reduce the collagen I, α-SMA and CTGF levels in the ovaries of the PCOS rats. Our results demonstrated that Rha significantly ameliorated metabolic disturbances and ovarian fibrosis in the letrozole-induced PCOS rats. Rha may be an effective compound in preventing ovarian fibrosis in the future.

Effects of Armillaria mellea polysaccharides (AMP) on letrozole-induced polycystic ovary syndrome (PCOS) in rats

Shumei Zhong,Wanying Li,Zixian Wang,Haokun Chen,Yaqi Wu,Chaojie Chong,Zhewen Dong,Jia-Le Song 한국식품영양과학회 2021 한국식품영양과학회 학술대회발표집 Vol.2021 No.10

To investigate the effects of AMP on letrozole-induced PCOS rats. All SD rats were randomly divided into normal, PCOS, metformin group (Met, 265 mg/kg) and AMP group (1 g/kg) and weighed every three days. After 21 days of continuous administration, oral glucose tolerance test was performed, and the blood glucose(BP) level was measured. After execution, calculate the relative weight of bilateral ovaries. Serum levels of FSH, LH, E2, T and FINS were detected by ELISA assay. Serum levels of TC, TG, HDL-C and LDL-C were detected by the kit and calculated the LH/FSH ratio and HOMA-IR level. Compared with the normal group, body weight and relative weight of ovaries, BP level and serum levels of T, LH, LH/FSH ratio, FINS, HOMA-IR, TC, TG, LDL-C, and E2, FSH, HDL-C in model group increased. Compared with the PCOS group, body weight in the Met group and AMP group was lower, BP level and serum levels of T, LH, FINS, HOMA-IR and LDL-C in the Met group and AMP group were significantly lower and the level of HDL-C was higher. Armillaria mellea polysaccharides can improve hormone disturbance, glucose metabolism and abnormal lipid level in letrozole-induced polycystic ovary syndrome model rats.

Protective effect of centaureidin-3,5-O-biglucoside (C3G) on 5-fluorouracil(5-FU)-induced mucositis in BALB/c mice

Junyang Liu,Zixian Wang,Wanying Li,Zhewen Dong,Chaojie Chong,Yaqi Wu,Shumei Zhong,Haokun Chen,Zhiqiang Liu,Qi Qi Pang,Jia-Le Song 한국식품영양과학회 2021 한국식품영양과학회 학술대회발표집 Vol.2021 No.10

To explore the effect of C3G on intestinal mucosal injury in 5-FU induced BALB/c mice. Mice were randomly divided into normal, model and C3G groups. After the last administration, mice were anesthetized, and calculated the thymus and spleen indexes. The ileum was used to stain with H&E observation. Serum levels of IL-1β, IL-6, IL-10 and TNF-α were determined by ELISA kits. Compared with the normal group, the model group had diarrhea, body weight and thymus and spleen index decreased significantly, intestinal villus epithelial cells fell off, crypt structure was destroyed, and inflammatory cell infiltration was observed. The body weight, thymus and spleen indexes of the treatment group and the prevention group decreased less, and the levels of TNF-α, IL-1β and IL-6 were significantly decreased, and the level of IL-10 was significantly increased relative to the model group. The intestinal villi structure was relatively complete, and a small amount of crypt structure was destroyed, with a small amount of inflammatory cell infiltration, which reduced the mucosal damage. The administration of C3G could protect intestinal mucosa from 5-FU-induced injury.