사염화탄소 투여 흰쥐에서 간섬유화 진행에 따른 Propranolol 의 약동학적 지표들의 변화

이민호,강주섭,강문수,윤병철,이오영,함준수,변재원,윤창옥 대한간학회 2001 Clinical and Molecular Hepatology(대한간학회지) Vol.7 No.2

Background/Aims : This study was designed to determine the effect of hepatic fibrotic severity on pharmacokinetics of propranolol in CC1q - treated rats. Methods - 1 mL/kg of 10% CC14 in olive oil was injected intramuscularly to rats twice weekly for 4, 6, 8 and 10 weeks, respectively (n=6). Control (n=6) was a sham - injected equal dose of olive oil for 10 weeks. After intravenous bolus injection of 2 mg/kg propranolol to rats, the serum propranolol concentrations were analyzed for 4 hours at various time points by a HPLC - fluorimetric system, and pharmacokinetic parameters such as Co, MRT, AUC, Vdss, tl/2(p) and CLp were determined. Then, a small amount of hepatic tissue was obtained and subjected to determination of the hepatic 4 - hydroxyproline content, which confirmed the hepatic fibrotic severity. Results : The serum concentrations of propranolol at 0.5, 1, 2 and 4 hours were significantly increased in CC1q-treated rats (p$lt;0.01). In proportion to the duration of CC4 treatment, Co and AUC were significantly increased, and Vdss and CLp were significantly decreased (p$lt;0.001). But MRT and t1/2(β) were not significantly changed. The hepatic 4 -hydroxyproline content was gradually increased in CC4-treated rats (p$lt;0.001). Conclusion - Gradual changes in pharmacokinetic parameters of propranolol were seen to be dependent on the hepatic fibrotic severity. We suggest that gradual dosage modification, according to their hepatic fibrotic severity, is necessary for many drugs administered to patients with chronic liver disease.(Korean J Hepatol 2001;7:181-188)

물아닌 용매-비전해질 계의 비에너지론적인 연구 : The Sticking-Slipping Phenomena of Solvents 용매의 Sticking-Slipping현상

尹昌柱 聖心女子大學校 1977 論文集 Vol.8 No.-

요소, 1,3-디메틸요소, 아세타미드 및 프로피온아미드에 대한 0.2m 농도까지의 점도와 삼투계수를 25℃와 45℃에서 H₂O, MeOH, EtOH 및 DMSO 용액에서 측정하였다. 점도 B 와 C 계수를 Jones-Dole 방정식에서 구하였고 이들 값들을 이미 알려진 용매구조의 개념을 기초로 고찰하였다. 용매에 용질이 녹는데 따르는 변화는 그 변화가 양의 값 또는 부의 값인가에 따라 설명될 수 있다. 또한 새로운 모형 "Sticking-Slipping현상"으로도 설명될 수 있다. Viscosity coefficient measurements at concetrations up to 0.2m for urea, 1,3-dimethylurea, acetamide, and propionamide are reported in H₂O, MeOH, EtOH and DMSO at 25˚and 45℃. The viscosity B and C coefficients are obtained from the Jones-Dole equation and are discussed, based on the concept of solvent structure. The change that accompanies the dissolution of a solute in solvent can be explained whether that change is positive or negative. The structural change can also be explanied with the new model. "The Sticking-Slipping Phenomena".

Lipids-Induced Exosomal lincRNA-ROR Released from Hepatocellular Carcinoma Cell Promotes Tumor Cell Growth through the PI3K/Akt Pathway

( Eun Ju Cho ),( Young Chang ),( Hyoyeong Lee ),( Jeong-hoon Lee ),( Su Jong Yu ),( Yoon Jun Kim ),( Jung-hwan Yoon ) 대한간학회 2018 춘·추계 학술대회 (KASL) Vol.2018 No.1

Aims: The lipid-related metabolic dependence is a hallmark of cancer. Exosomes in tumor microenvironment are enriched in lipids and their accumulation in cells might modulate recipient cell homeostasis. We investigated whether lipids and lipids-induced exosomes released from HCC cells can induce a tumor-promoting phenotype and the underlying mechanisms. Methods: Human HCC cell lines (Huh-7, SNU-761, and SNU-3058) were incubated with oleic acid (OA) or control vehicle. The released exosomes were isolated, quantified, and applied to HCC cells. Results: Incubation of HCC cells with OA enhanced proliferation, migration and invasion of cells. Furthermore, OA upregulated long noncoding RNA regulator of reprogramming (lincRNA-ROR) mRNA expression in Huh-7 and SNU-3058 cells, whereas its expression in SNU-761 cells did not change. Exosomes collected form OA-treated cells were enriched with lincRNA-ROR mRNA, and upregulated proliferation and invasion of HCC cells through the activation of PI3K/Akt pathway. Conclusions: These findings suggest that lipids-induced exosomal lincRNA-ROR released from hepatocellular carcinoma cell promotes tumor cell growth through the PI3K/Akt pathway.

Lipids Induce Release of Tumor-Promoting Exosomes from Hepatocellular Carcinoma Cells

( Eun Ju Cho ),( Hyo Yeong Lee ),( Joon Yeul Nam ),( Young Chang ),( Hyeki Cho ),( Young Youn Cho ),( Jeong-hoon Lee ),( Su Jong Yu ),( Yoon Jun Kim ),( Jung-hwan Yoon ) 대한간학회 2017 춘·추계 학술대회 (KASL) Vol.2017 No.1

Aims: Hypoxia enhances lipid droplets accumulation by HIF-dependent mechanism, leading to intra-tumoral fatty metamorphosis which is one of the characteristics of early-stage hepatocellular carcinoma (HCC). We investigated whether lipids and lipids-induced exosomes released from HCC cells can induce a tumor-promoting phenotype. Methods: Human HCC cell lines (Huh-7, SNU-761, and SNU-3058) were incubated with oleic acid (OA) or control vehicle. The released exosomes were isolated, quantified, and applied to HCC cells. Results: Incubation of Huh-7 and SNU-761 cells with OA increased proliferation and migration of cells in a fatty-acid binding protein 3 (FABP-3) dependent manner, whereas SNU-3058 cells did not respond to OA. Furthermore, OA upregulated FABP-3 mRNA expression in Huh-7 and SNU-761 cells, whereas its expression in SNU-3058 cells did not change. OA enhanced release of exosomes from Huh-7 and SNU-761 cells, and exosomes collected form these cells upregulated proliferation and invasion of cells. However, this was not observed in SNU-3058 cells. Exosomes released from OA-treated cells were reduced by the inhibition of rho-associated, coiled-coil-containing protein kinase 1 (ROCK1) or FABP-3. Conclusions: These findings suggest that lipids induce release of exo-somes containing unique cargoes from HCC cells in a FABP-3 dependent manner, which may induce the progression of HCC.

배추種子 發芽에 미치는 鹽素化炭化水素의 影響

尹昌柱,朴敏哲 聖心女子大學校 1981 論文集 Vol.12 No.2

Trichlorfon이 배추種子의 發芽에 미치는 影響을 調査하였다. 發芽率은 1g/ℓ이상의 濃度에서는 抑制되었으나 0.1g/ℓ이하의 濃度에서는 전혀 影響이 없었다. 줄기와 뿌리의 伸長生長은 0.01g/ℓ의 低濃度處理區에서는 큰 影響이 없었으나 그 이상의 濃度에서는 生長의 低下作用이 나타났고 生量의 變化도 伸長生長과 같은 傾向이 나타났으나 乾量에 있어서는 全般的으로 時間에 따라 減少하는 傾向을 보였다. 葉綠素는 모든 處理區에서 그 形成이 抑制되었으며 水分含量도 處理된 濃度에 反比例하여 增加하는 傾向이 있었으나 0.01g/ℓ의 處理區에서는 對照區와 큰 차이가 없었다. 蛋白質과 RNA는 0.1g/ℓ와 1g/ℓ의 處理區에서는 對照區와 0.01g/ℓ의 處理區에서 보다 더 낮은 含量을 보였다. The effects of Trichlorfon on the germination of chinese cabbage seeds were investigated. Trichlorfon inhibited the germination when seeds were treated at the concentrations of 1g/ℓor more, but made no difference at the concentrations of 0.1 and 0.01g/ℓ. Growth of shoot and root length was not affected by the treatment of Trichlorfon at 0.01g/ℓ, but was inhibited at 0.1 and 1g/ℓ. Chlorophyll formation was severely inhibited by Trichlorfon treatments. Fresh weights and water contents were in inverse proportion to the treated concentrations, but the water contents of control and 0.01g/ℓ didn't show any distinct difference. Contents of protein and RNA were lower in germinating seeds at the treated concentration of 0.1 and 1g/ℓthan at that of control and 0.01g/ℓ.

HIV-1 역전사효소 억제제의 구조화 활성 사이의 정량적 상관관계 연구 : HEPT와 TIBO 유도체 HEPT and TIBO Derivatives

윤은진,조효영,윤창주 가톨릭대학교 자연과학연구소 2003 자연과학논문집 Vol.24 No.-

본 연구는 QSAR을 이용하여 HIV-1 RT의 한 종류인 TIBO와 HEPT의 구조와 활성간의 상관관계를 설명하였다. 좋은 QSAR 모델은 통계적인 수치와 예측 정도를 모두 만족시켜야 한다. 생성된 모델과 수식을 통해 HEPT 유도체는 소수성이 활성과 관련이 있는 반면, TIBO 유도체는 소수성과 비편재화, 물 굴절도가 활성도와 관련이 있음을 알 수 있었다. 이러한 결과는 TIBO와 HEPT 유도체의 구조적인 특징을 파악하는데 유용하며, HIV-1 RT 억제 과정의 이해를 돕는다. 따라서, 본 연구를 통해 좀 더 강력하고 새로운 HIV-1 RT inhibitor를 제안할 수 있다. Quantitative structure-activity relationship(QSAR) have been applied in order to explain the structure requirements of HIV-1 RT inhibitory activity of TIBO and HEPT derivatives. The best QSAR models are satisfactory in both statistical significance and predictive ability. The derived structural descriptors for HEPT indicate the importance of hydrophobic contributions toward the HIV-1 RT inhibition, but the descriptors for TIBO indicate the importance of hydrophobic, electronic and molar refractivity contribution toward the HIV-1 RT inhibition. These results can be useful in identifying the structural requirements of TIBO and HEPT derivatives and helpful for better understanding the HIV-1 RT inhibition. Eventually, they provide a beneficial basis to design new and more potent inhibitors of HIV-1 RT.

자치행정에서 자원적 영역의 활용에 관한 연구 : 충남·대전지역사례를 중심으로 Focused on Chungnam and Taejon Region

윤주명,황창순 순천향대학교 사회과학연구소 1997 순천향 사회과학연구 Vol.3 No.1

물-알코올 혼합용액의 ^(17)O-핵 스핀-스핀 이완연구

윤창주,김정 가톨릭대학교 자연과학연구소 1990 자연과학논문집 Vol.11 No.-

알코올 수용액에서 물과 알코올의 OH-기의 상관관계시간을 분리하여 측정하였다. 알코올의 수산화 기에 대한 결과를 알킬기의 것과 비교하여 알코올 분자 내에서의 재배향이 어떻게 일어나는 가에 대한 정보를 얻었다. The corelational times of OH-group of water and alcohols were determined seperately. The results for the hydroxylgroup of alcohols were compared with those for the alkyl part, in order to obtain the information about profiles of the internal reorientations in alcohol melecules.

티오아세트아미드와 디메틸아세트아미드 사이의 분자간 상호작용에 미치는 용매효과

윤창주,최영상 가톨릭대학교 자연과학연구소 1988 자연과학논문집 Vol.10 No.-

DMA-CCl₄와 DMA-CH₃CN 혼합용매에서 TA의 아미노기의 용매이동과 선모양을 혼합용매의 조성의 함수로 측정하였다. -NH₂기의 양성자 선 모양에서 TA는 CCl₄와 같은 비극성 용액 계에서 N-H…S=C 결합을 통하여 자체회합을 하고 있음이 발견되었다. 용매이동의 값으로 부터 TA와 DMA 사이의 수소결합에 대한 평형상수는 CCl₄와 CH₃CN용액 계에서 각각 9.22ㆍ10^(-2) 및 1.96ㆍ10^(-1)이었다. The solvent shifts of the amino group of TA in DMA-CCI₄ and in DMA-CH₃CN mixed solvents have been measured as a function of compositions of the mixed solvents. From the nmr line shapes of NH₂ group, it was shown that TA molecules self-associate through N-H…S=C bond in non-polar CCI₄ solvents. And from the solvent shifts the equilibrium constants for the H-bonding between TA and DMA have been calculated. The equilibrium constants were 9.22·10^(-2) and 1.96·10^(-1) in CC1₄ and in CH₃CN, respectively.

한국 하천수에서의 유기물분해의 에너지론적 연구

윤창주,권영순 聖心女子大學校 1980 論文集 Vol.11 No.2