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- 저자 : Chanchao, Chanpen (검색결과 8 건)
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Atsalek Rattanawannee,Chanpen Chanchao,Siriwat Wongsiri 한국응용곤충학회 2012 Journal of Asia-Pacific Entomology Vol.15 No.4
Geometric morphometry was used to characterize 73 Apis dorsata colonies collected from 31 different localities in five major geographic regions of mainland Thailand. We measured 19 easily identified landmarks from the digitized images of the right forewing of 10 worker bees from each colony (730 bees in total);thus, avoiding the confounding variation from haploid or diploid males. After plotting the factor scores,A. dorsata from (mainland) Thailand were found to belong to a single group, which was further supported by a hierarchical cluster analysis-generated dendrogram. Multivariate analysis of variance (MANOVA, α=0.05) demonstrated no significant differences among the five geographic groups of A. dorsata in Thailand,producing a low degree of accuracy (31.2%) in the identification of the geographic region from which any individual bee originated. Additionally, when the bee samples were classified into two groups, those north and south of the Isthmus of Kra were not significantly different (MANOVA, α=0.05), and a low rate of correct classification in a cross-validation test (65% correct) was found. Therefore, this geometric morphometric based analysis of worker bee wing venation pattern suggests that A. dorsata populations in mainland Thailand are panmictic.
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Kijpornyongpan, Teeratas,Sereemaspun, Amornpun,Chanchao, Chanpen Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.4
Background: Transient receptor potential melastatin 8 (TRPM8), a principle membrane receptor involved in calcium ion influx and cell signal transduction, has been found to be up-regulated in some cancer types, including melanomas. Efficiency of menthol, an agonist of TRPM8, in killing melanoma cancer cells has been reported previously, but the mechanisms remain unclear. We here determined whether in vitro cytotoxic effects of menthol on A-375 human malignant melanoma cells might be related to TRPM8 transcript expression. Materials and Methods: The $PrestoBlue^{(R)}$ cell viability assay was used to assess the in vitro cytotoxic effect of menthol after 24h of treatment. RT-PCR was used to quantify TRPM8 transcript expression levels in normal and menthol-treated cells. Cell morphology was observed under inverted phase contrast light microscopy. Results: TRPM8 transcript expression was found at low levels in A-375 cells and down-regulated in a potentially dose-dependent manner by menthol. Menthol exerted in vitro cytotoxic effects on A-375 cells with an $IC_{50}$ value of 11.8 ${\mu}M$, which was at least as effective as 5-fluorouracil ($IC_{50}=120{\mu}M$), a commonly applied chemotherapeutic drug. Menthol showed no dose-dependent cytotoxicity on HeLa cells, a TRPM8 non-expressing cell line. Conclusions: The cytotoxic effects on A-375 cells caused by menthol might be related to reduction of the TRPM8 transcript level. This suggests that menthol might activate TRPM8 to increase cytosolic $Ca^{2+}$ levels, which leads to cytosolic $Ca^{2+}$ imbalance and triggers cell death.
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Expression and characterization of Apis dorsata α-glucosidase III
Manlika Kilaso,Chanpen Chanchao,Jirattikarn Kaewmuangmoon,Aphichart Karnchanatat,Polkit Sangvanich 한국응용곤충학회 2011 Journal of Asia-Pacific Entomology Vol.14 No.4
α-Glucosidase III plays a major role in producing the monosaccharides in honey, mainly glucose and fructose,from sucrose in plant nectar. The honey from Apis dorsata is different and somewhat acidic compared to that of A. mellifera. The transcript expression level of the α-glucosidase III gene was evaluated by RT-PCR on total RNA extractions from eggs, larvae, pupae, and foraging imagoes. The highest expression level was found in foragers, which concurs with its biological function in honey bees as only foragers make honey. Using primers designed from the sequence of the A. mellifera α-glucosidase III gene, the full length ORF of the cDNA (1704 bp including the stop codon and 567 predicted amino acids) was obtained by RT-PCR of a total RNA preparation from foragers, and direct sequenced. BLASTn analysis revealed that the cDNA sequence showed the highest sequence similarity to the A. mellifera α-glucosidase III gene (96%). Enrichment of the native α-glucosidase III protein from forager bees was performed by ammonium sulfate precipitation at 95% saturation followed by diethylaminoethyl-cellulose ion exchange column chromatography of the unprecipitated fraction (yield and specific activity of 3.95 U/mg and 118.5%) followed by Superdex 75 ion exchange column chromatography to give a main protein and sole α-glycosidase activity band of ~63 kDa, with a yield of 5.57 U/mg and a specific activity of 19.5%. This enriched fraction had optimal pH, temperature, and substrate concentration for α-glucosidase activity of 4.5, 35 °C and 55 °C, and 50 mM maltose, respectively.
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Progress of Inter-species Somatic Cell Nuclear Transfer in Bovidae and Felidae Family
Rangsun Parnpal,Sumeth Insoonthornruksa,Kanokwan Srirattana,Chanchao Lorthongpanich,Anawat Sangmalee,Nucharin Sripunya,Kwanrudee Keawmungkun,Wanwisa Phewsoi,Chuti Laowtammathron,Wanchai Tunwattana,Wac 한국동물번식학회 2009 Reproductive & Developmental Biology(Supplement) Vol.33 No.2s
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Ittiudomrak, Teeranai,Puthong, Songchan,Roytrakul, Sittiruk,Chanchao, Chanpen Korean Society of ToxicologyKorea Environmental Mu 2019 Toxicological Research Vol.35 No.2
Ovarian cancer is the fifth main cause of pre-senescent death in women. Although chemotherapy is generally an efficient treatment, its side effects and the occurrence of chemotherapeutic resistance have prompted the need for alternative treatments. In this study, ${\alpha}$-mangostin and apigenin were evaluated as possible anticancer alternatives to the chemotherapeutic drug doxorubicin, used herein as a positive control. The ovarian adenocarcinoma cell line SKOV-3 (ATCC No. HTB77) was used as model ovarian cancer cells, whereas the skin fibroblast line CCD-986Sk (ATCC No. CRL-1947) and lung fibroblast line WI-38 (ATCC No. CCL-75) were used as model untransformed cells. Apigenin and doxorubicin inhibited the growth of SKOV-3 cells in a dose- and time-dependent manner. After 72 hr exposure, doxorubicin was mostly toxic to SKOV-3 cells, whereas apigenin was toxic to SKOV-3 cells but not CCD-986Sk and WI-38 cells. ${\alpha}$-Mangostin was more toxic to SKOV-3 cells than to CCD-986Sk cells. A lower cell density, cell shrinkage, and more unattached (floating round) cells were observed in all treated SKOV-3 cells, but the greatest effects were observed with ${\alpha}$-mangostin. With regard to programmed cell death, apigenin caused early apoptosis within 24 hr, whereas ${\alpha}$-mangostin and doxorubicin caused late apoptosis and necrosis after 72 hr of exposure. Caspase-3 activity was significantly increased in ${\alpha}$-mangostin-treated SKOV-3 cells after 12 hr of exposure, whereas only caspase-9 activity was significantly increased in apigenin-treated SKOV-3 cells at 24 hr. Both ${\alpha}$-mangostin and apigenin arrested the cell cycle at the $G_2/M$ phase, but after 24 and 48 hr, respectively. Significant upregulation of BCL2 (apoptosis-associated gene) and COX2 (inflammation-associated gene) transcripts was observed in apigenin- and ${\alpha}$-mangostin-treated SKOV-3 cells, respectively. ${\alpha}$-Mangostin and apigenin are therefore alternative options for SKOV-3 cell inhibition, with apigenin causing rapid early apoptosis related to the intrinsic apoptotic pathway, and ${\alpha}$-mangostin likely being involved with inflammation.
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Teeranai Ittiudomrak,Songchan Puthong,Sittiruk Roytrakul,Chanpen Chanchao 한국독성학회 2019 Toxicological Research Vol.35 No.2
Ovarian cancer is the fifth main cause of pre-senescent death in women. Although chemotherapy is generally an efficient treatment, its side effects and the occurrence of chemotherapeutic resistance have prompted the need for alternative treatments. In this study, α-mangostin and apigenin were evaluated as possible anticancer alternatives to the chemotherapeutic drug doxorubicin, used herein as a positive control. The ovarian adenocarcinoma cell line SKOV-3 (ATCC No. HTB77) was used as model ovarian cancer cells, whereas the skin fibroblast line CCD- 986Sk (ATCC No. CRL-1947) and lung fibroblast line WI-38 (ATCC No. CCL-75) were used as model untransformed cells. Apigenin and doxorubicin inhibited the growth of SKOV-3 cells in a dose- and time-dependent manner. After 72 hr exposure, doxorubicin was mostly toxic to SKOV-3 cells, whereas apigenin was toxic to SKOV-3 cells but not CCD-986Sk and WI-38 cells. α-Mangostin was more toxic to SKOV-3 cells than to CCD-986Sk cells. A lower cell density, cell shrinkage, and more unattached (floating round) cells were observed in all treated SKOV-3 cells, but the greatest effects were observed with α-mangostin. With regard to programmed cell death, apigenin caused early apoptosis within 24 hr, whereas α-mangostin and doxorubicin caused late apoptosis and necrosis after 72 hr of exposure. Caspase-3 activity was significantly increased in α-mangostin-treated SKOV-3 cells after 12 hr of exposure, whereas only caspase-9 activity was significantly increased in apigenin-treated SKOV-3 cells at 24 hr. Both α-mangostin and apigenin arrested the cell cycle at the G2/M phase, but after 24 and 48 hr, respectively. Significant upregulation of BCL2 (apoptosis-associated gene) and COX2 (inflammation-associated gene) transcripts was observed in apigenin- and α-mangostin-treated SKOV-3 cells, respectively. α-Mangostin and apigenin are therefore alternative options for SKOV-3 cell inhibition, with apigenin causing rapid early apoptosis related to the intrinsic apoptotic pathway, and α-mangostin likely being involved with inflammation.
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Kustiawan, Paula M,Phuwapraisirisan, Preecha,Puthong, Songchan,Palaga, Tanapat,Arung, Enos T,Chanchao, Chanpen Asian Pacific Journal of Cancer Prevention 2015 Asian Pacific journal of cancer prevention Vol.16 No.15
Background: Previously, stingless bee (Trigona spp.) products from East Kalimantan, Indonesia, were successfully screened for in vitro antiproliferative activity against human cancer derived cell lines. It was established that propolis from T. incisa presented the highest in vitro cytotoxicity against the SW620 colon cancer cell line (6% cell survival in $20{\mu}g/mL$). Materials and Methods: Propolis from T. incisa was extracted with methanol and further partitioned with n-hexane, ethyl acetate and methanol. The in vitro cytotoxicity of the extracts was assessed by the MTT assay against human colon (SW620), liver (Hep-G2), gastric (KATO-III), lung (Chago) and breast (BT474) cancer derived cell lines. The active fractions were further enriched by silica gel quick column, absorption and size exclusion chromatography. The purity of each fraction was checked by thin layer chromatography. Cytotoxicity in BT-474 cells induced by cardanol compared to doxorubicin were evaluated by MTT assay, induction of cell cycle arrest and cell death by flow cytometric analysis of propidium iodide and annexin-V stained cells. Results: A cardol isomer was found to be the major compound in one active fraction (F45) of T. incisa propolis, with a cytotoxicity against the SW620 ($IC_{50}$ of $4.51{\pm}0.76{\mu}g/mL$), KATO-III (IC50 of $6.06{\pm}0.39{\mu}g/mL$), Hep-G2 ($IC_{50}$ of $0.71{\pm}0.22{\mu}g/mL$), Chago I ($IC_{50}$ of $0.81{\pm}0.18{\mu}g/mL$) and BT474 (IC50 of $4.28{\pm}0.14{\mu}g/mL$) cell lines. Early apoptosis (programmed cell death) of SW620 cells was induced by the cardol containing F45 fraction at the $IC_{50}$ and $IC_{80}$ concentrations, respectively, within 2-6 h of incubation. In addition, the F45 fraction induced cell cycle arrest at the G1 subphase. Conclusions: Indonesian stingless bee (T. incisa) propolis had moderately potent in vitro anticancer activity on human cancer derived cell lines. Cardol or 5-pentadecyl resorcinol was identified as a major active compound and induced apoptosis in SW620 cells in an early period (${\leq}6h$) and cell cycle arrest at the G1 subphase. Thus, cardol is a potential candidate for cancer chemotherapy.
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