참깨박의 재활용방안 : 제2보, 알칼리 및 염수추출법에 의한 분리참깨단백의 제조 2. Manufacturing of Isolated Sesame Protein by Alkali and Salt Extraction Method

이근보,김경이,구본순,김덕숙 瑞逸專門大學 1993 論文集 Vol.11 No.-

참깨탈지박(DSM)으로부터 알칼리(AEM) 및 염수추출법(SEM)에 의하여 분리참깨단백(ISP)을 제조한 결과 회수율은 각각 43.06, 41.13%였으며, ISP의 조단백 함량은 각각 90.14, 91.08%였다. 현째까지 알려진 바로는 AEM이 가장 적합한 추출방법인 것으로 알려지고 있으나, 회수율 및 단백함량 등을 종합적으로 고려할 때, SEM이 우수한 것으로 밝혀졌다. 이들 ISP의 아미노산 조성은 필수 아미노산중 Isoleucine, Lysine이 FAO 권장치 이하였으며, Lysine 함량은 FAO 권장치의 57-64%에 그쳐 제한 아미노산으로 밝혀졌다. We are manufactured isolated sesame protein(ISP) from defatted sesame meal used for extraction method by alkali(AEM) and saline solution(SEM). The recovery yields were 43.06, 41.13%, and crude protein content of ISP were 90.14, 91.08%, respectively. AEM was reported the most suitable extraction method. But in the case of consideration the recovery yield and crude protein content, SEM was a excellent extraction method. Amino acid composition of ISP was represented the low level to isoleucine and lysine than FAO reference pattern. Expecially, lysine was a limiting amino acid, the content was about 57-64% level of FAO reference pattern.

북한 임상검사인력에 대한 고찰

Bon-Kyeong Koo,Sei Ick Joo,Dai-Joong Kim,In-Ho Jang 대한임상검사과학회 2020 대한임상검사과학회지(KJCLS) Vol.52 No.1

There is a noticeable gap in the personnel structures of clinical laboratories between North Korea and South Korea. In North Korea, ‘Laboratory Doctor’ is similar to the workforce of ‘Medical Technologist (commonly known as Clinical Laboratory Technologist or Medical Laboratory Scientist)’ in South Korea. Considering preceding research based on the verbal evidence of North Korean healthcare personnel defectors, it is estimated that the status of laboratory doctor in North Korea generally corresponds to physician and feldsher (such as physician assistant in Western countries). Physicians and feldshers are trained and fostered for five and a half years in medical universities and for three years in medical vocational schools (so-called junior college of medicine). Unlike South Korea, the North Korea’s healthcare personnel system does not subdivide the tasks, education, qualifications and law regarding the specialties of health experts. It is thought that the Korean Association of Medical Technologists needs to collaboratively search and present the milestones for establishment of a professional system on clinical laboratory personnel in North Korea through cooperative research on policies with the related organizations for better preparation of the unification of the Korean Peninsula. 북한과 남한은 임상검사실 인력구조에서 차이가 있다. 북한에서는 남한의 ‘임상병리사’에 해당하는 유사인력을 ‘실험의사(검사의사)’라고 호칭한다. 본 연구자들은 탈북 보건의료인의증언을 바탕으로 한 선행연구를 고찰해 보면, 검사의사(laboratory doctor)의 신분은 의사(physician)와 준의(feldsher, physician assistant)일 것이라고 사료된다. 의사와 준의는 5.5년제 의학대학 및 3년제 의학전문학교에서 양성되고있다. 북한의 보건의료인력체계는 남한의 경우처럼 보건전문가들의 전문영역별 업무나 교육, 자격 및 법률이 세분화되어 있지않다. 대한임상병리사협회는 한반도 통일을 대비하여 유관 단체와 함께 정책연구를 통해서 북한 임상검사인력에 대한 직제전문화 구축과 발전 방향을 상호 모색해야 할 것으로 사료된다.

의료기사의 의료인 종별 포함에 관한 기초조사 연구: 한국, 일본, 대만을 중심으로

Bon-Kyeong KOO,Chang-Eun Park 대한임상검사과학회 2024 대한임상검사과학회지(KJCLS) Vol.56 No.1

본 연구의 목적은 의료기사를 의료인 종별에 포함시키는 것에 대한 기초 자료를 제시하는 것이다. 의료법에서 의료인을 의사, 치과의사, 한의사, 조산사, 간호사로 정의한다. 의료기사는 임상병리사, 방사선사, 물리치료사, 작업치료사, 치과기공사, 치과위생사로 구분한다. 한국은 의료인에 의료기사를 포함하지 않지만 일본과 대만은 의료인으로 규정하고 있다. 국제표준직업분류(ISCO-08), 한국표준직업분류(KSCO-2017), 일본표준직업분류(JSOC-2009), 대만표준직업분류(TSOC-2010), 미국표준직업분류(SOC-2018) 등의 다양한 표준직업분류를 비교하였다. 의료기사 교육체계는 4년제 대학과 3년제 전문대학 프로그램을 포함하는 것으로 설명하였다. 의료행위, 치료, 진료보조 분야에서 의료기사의 역할을 개략적으로 설명했다. 이러한 기초자료는 의료기사의 의료인 종별 포함의 의미에 대한 논의의 필요성과 의료인 종별 포함과 관련하여 의료기사의 전문성의 합법화에 기여할 것이다. This study provides the underlying data on the inclusion of medical technologists in the category of medical personnel. Medical personnel are defined in the Medical Service Act as physicians, dentists, oriental medicine doctors, midwives, and nurses. In the act, medical technologists shall be classified into clinical laboratory technologists (clinical pathology technologists in the Korean language), radiological technologists, physical therapists, occupational therapists, dental technologists, and dental hygienists. Although South Korea’s medical personnel do not include medical technologists, Japan and Taiwan define them as medical personnel. Various standard occupational classification such as International Standard Classification of Occupations 2008, Korean Standard Classification of Occupations 2017, Japan Standard Occupational Classification 2009, Taiwan Standard Occupational Classification 2010, and USA Standard Occupational Classification 2018 were compared. The education system for medical health technologists was explained to include 4-year university and 3-year junior college programs. The roles of medical technologists in medical practice, therapy, and medical assistance were outlined. These basic materials incorporate the need for discussions about the meaning of including medical technologists in the type of medical personnel. These discussions will contribute to the legalization of medical technologists’ professionality with regard to their inclusion in the type of medical personnel.

임상병리사 명칭 변경을 위한 타당성 연구

구본경 ( Bon-kyeong Koo ),김원식 ( Won Shik Kim ),박선구 ( Sun Gu Park ),박종오 ( Jong O Park ),윤성민 ( Seong Min Yoon ) 대한임상검사과학회 2021 대한임상검사과학회지(KJCLS) Vol.53 No.1

임상병리사 명칭 변경과 관련한 의견을 수렴하기 위해 사단법인 대한임상병리사협회에서는 정회원으로 등록된 총 22,638명에게 온라인을 통해 제시된 명칭에서 2개를 선택하는 설문 답변을 요구하였으며 이 중 3,999명(17.66%)이 설문조사에 회신 답변하였다. 명칭 선호도는 회원 1인당 2개를 선택하는 답변을 통해서 6,958건이 산출되었으며 그 중 5,555건(79.83%)가 ‘분석사’ 레벨을 선호하였다. 명칭에 대한 조사결과는 진단검사분석사가 2,417건(34.73%)으로 가장 많았고, 임상검사분석사는 1,710건(24.57%), 의생명병리사는 758건(10.89%), 의생명분석사는 730건(10.49%), 의생명검사분석사는 730건(10.03%), 임상검사과학사는 646건(9.26%) 순으로 조사되었다. 따라서 조사된 회원들의 요구도, 자문, 문헌고찰, 표준직업분류, 주요 대상국 현황을 기반으로 “진단검사분석사(Diagnostic Laboratory Analyst), 의생명검사분석사(Biomedical Laboratory Analyst), 의생명분석사(Biomedical Analyst)”를 명칭 정립으로 제언하고자 한다. To investigate and accommodate opinions on the revision of the official occupational title of the medical technologist, the Korean Association of Medical Technologists (KAMT) requested 22,638 people registered as its regular members to participate in an online survey and select their two preferred options from the alternative job titles presented. Survey responses were collected from 3,999 people (17.66%). To examine job title preferences among the KAMT members, each respondent was asked to choose two terms from the choice set. As a result, 6,958 responses were obtained, and out of the total responses, 5,555 (79.83%) indicated a choice for a job title that included the word ‘analyst’ as the preferred alternative. The survey results showed that “Diagnostic Laboratory Analyst” was the most preferred alternative selected by the largest proportion of respondents (2,417 responses, 34.73%), followed by “Clinical Laboratory Analyst” (1,710 responses, 24.57%), “Biomedical Pathology Technologist” (758 responses, 10.89%), “Biomedical Analyst” (730 responses, 10.49%), “Biomedical Laboratory Analyst” (730 responses, 10.03%), and “Clinical Laboratory Scientist” (646 responses, 9.26%). Therefore, based on the responses of the surveyed members, results of consultation and literature review, the Standard Classification of Occupations (SCO), and the current status of the job titles used in major countries, it is suggested that the occupational title of medical technologists should be changed by adopting “Diagnostic Laboratory Analyst”, “Biomedical Laboratory Analyst”, or “Biomedical Analyst” as their new official job title. Copyright Ⓒ 2021 The Korean Society for Clinical Laboratory Science. All rights reserved.

TYLCV transmission by whiteflies, weeds and seeds

Kyeong-Yeoll Lee,Jae-Kyoung Shim,Sukchan Lee,Eui-Joon Kil,Gwan-Seok Lee,Hong-Soo Choi,Chang-Seok Kim,Jung-Hwan Lee,Ji-Kwang Kim,Kyung-Bon Koo 한국응용곤충학회 2017 한국응용곤충학회 학술대회논문집 Vol.2017 No.04

Tomato yellow leaf curl virus (TYLCV) causes a highly serious disease in tomatoes in many countries. The most important thing in management is the prevention of virus transmission. TYLCV had been known to be only transmitted by a vector, the sweetpotato whitelfy, Bemisia tabaci. However, we identified two other important routes of TYLCV transmission into tomatoes. It is seed-transmissible. Many seeds available in domestic and global markets were infected by TYLCV. It is also infective into various weeds and other horticultural crops and transmit virus through vector insects. Therefore, we need a new strategy for the virus and vector management.

Mind Bomb-2 Is an E3 Ligase for Notch Ligand

Koo, Bon-Kyoung,Yoon, Ki-Jun,Yoo, Kyeong-Won,Lim, Hyoung-Soo,Song, Ran,So, Ju-Hoon,Kim, Cheol-Hee,Kong, Young-Yun American Society for Biochemistry and Molecular Bi 2005 The Journal of biological chemistry Vol.280 No.23

<P>The zebrafish gene, <I>mind bomb</I> (<I>mib</I>), encodes a protein that positively regulates of the Delta-mediated Notch signaling. It interacts with the intracellular domain of Delta to promote its ubiquitination and endocytosis. In our search for the mouse homologue of zebrafish <I>mind bomb</I>, we cloned two homologues in the mouse genome: a mouse orthologue (mouse <I>mib1</I>) and a paralogue, named <I>mind bomb-2</I> (<I>mib2</I>), which is evolutionarily conserved from <I>Drosophila</I> to human. Both Mib1 and Mib2 have an E3 ubiquitin ligase activity in their C-terminal RING domain and interact with <I>Xenopus</I> Delta (XD) via their N-terminal region. Mib2 is also able to ligate ubiquitin to XD and shift the membrane localization of Delta to intracellular vesicles. Importantly, Mib2 rescues both the neuronal and vascular defects in the zebrafish <I>mib<SUP>ta52b</SUP></I> mutants. In contrast to the functional similarities between Mib1 and Mib2, <I>mib2</I> is highly expressed in adult tissues, but almost not at all in embryos, whereas <I>mib1</I> is abundantly expressed in both embryos and adult tissues. These data suggest that Mib2 has functional similarities to Mib1, but might have distinct roles in Notch signaling as an E3 ubiquitin ligase.</P>

QUALITY IMPROVEMENT OF MUSTARD POWDER AND RENEWABLE PROJECT OF MUSTARD OIL

Kim, Kyeong-Yee,Koo, Bon-Soon,Lee, Keun-Bo 瑞逸專門大學 1990 論文集 Vol.8 No.-

본 연구는 겨자분말의 독특한 매운맛을 극대화시키고, 이의 제조공정상에서 얻어지는 기름을 식용으로 재활용하고자 시도하였다. 겨자분말의 매운맛에 영향을 미치는 중요한 인자는 잔류 수분 및 기름함량, 아미노산 조성 등으로 밝혀졌고 가열 전처리 없이 원두를 분쇄하고 용매추출법에 의하여 완전 탈지시킨 다음 역으로 이에 기름을 분무하여 기름함량 15.8%, 수분함량 5.7%의 분말을 조제하여 최대의 매운맛을 갖는 겨자분말을 얻을 수 있었다. 또한, 이 과정에서 얻어진 겨자원유를 완전 정제하여 얻은 정제 겨자유는 erucic acid 함량이 높아 단점으로 지적 되었으며, 조리용, 튀김용으로는 부적합 하였으나 김구이용 또는 혼합유의 원료유로서는 적합하여 이의 실용화 필요성을 제시하게 되었다. It was performed for the sake of the renewable project of oil obtained in this manufacturing process as well as improvement of the characteristic pungent taste in mustard powder in this study. The important influencing factors in the pungent taste of mustard powder were the residual moisture and oil content, and amino acid composition etc. Therefore we obtained defatted mustard meal (DMM) by solvent extraction from mustard seed powder. Its mustard powder product had the maximum pungent taste when this DMM was controlled the oil content 15.83% by spray of oil and moisture 5.7%. In addition, refined mustard oil obtained at this process had not only a disadvantage because of very high erucic acid content but also insuitability as cooking frying oil. But it was very good oil for season seaweed and as composition the oil of blended oil. At the result, mustard oil must be use for edible oil necessarily so.

논문 : 생물생산시설 및 환경공학 ; 은 나노 콜로이드를 이용한 돼지분뇨의 악취 저감 효과

김구필 ( Koo Pil Kim ),최영수 ( Young Soo Choi ),오광현 ( Kwang Hyun Oh ),구경본 ( Kyung Bon Koo ),서상룡 ( Sang Ryong Suh ),유수남 ( Soo Nam Yoo ),이경환 ( Kyeong Hwan Lee ) 한국농업기계학회 2011 바이오시스템공학 Vol.36 No.5

The effect of SNC(silver nano colloid) on the emission reduction of odors such as ammonia (NH3), hydrogen sulfide (H2S), and methane (CH4) from swine excreta was studied. Silver has been used as an universal antibiotic substance and can reduce the emission of some gases by sterilizing action. Therefore, an apparatus which produces SNC was developed and was conducted its performance test. Also, the SNC made by the apparatus was applied to swine excreta sampled from a piggery in oder to find the effect on the reduction of odor emission. An electrolysis apparatus was developed to produce SNC and its capacity was 0.024 ppm/hr·ℓ. The effects of SNC on the reduction of odor emission from swine excreta were tested for bad smell gases of ammonia (NH3), hydrogen sulfide (H2S) and methane (CH4). For ammonia gas, factorial experiments were conducted to find the effects of concentration and application rate of SNC. The test results for the different concentrations of 20 ppm, 50 ppm, and 100 ppm showed that the more concentration of SNC was increased, the more emission reduction of ammonia gas increased. From the test results about the effect of application rate, the more SNC was applied, the more emission reduction of NH3 increased. In order to reduce the concentration of NH3 below 5 ppm, SNC of 50 ppm is recommended to be applied at an interval of 6 hours, and is mixed with swine excreta in the volumetric ratio of 4:1. For hydrogen sulfide gas, the concentration was decreased as time went by and was reduced rapidly in the first stage of the tests for all applied concentrations of SNC (20 ppm, 50 ppm, and 100 ppm). Especially, when 100 ml of SNC with 100 ppm was applied, emission of hydrogen sulfide gas was reduced rapidly during early 4 hours after the application of SNC. And, concentration of hydrogen sulfide gas was maintained below 20 ppm after 12 hours. For methane gas, t-test showed that there was no significance on the effect of its application for all applied concentrations of SNC. Therefore, it was concluded that the application of SNC on swine excreta had no effect on the emission reduction of CH4.

Experimental Research Article : Amino acid residues involved in agonist binding and its Linking to channel gating, proximal to transmembrane domain of 5-HT3A receptor for halothane modulation

( Mi Kyeong Kim ),( Kyeong Tae Min ),( Bon Nyeo Koo ) 대한마취과학회 2009 Korean Journal of Anesthesiology Vol.56 No.1

Background: The 5-hydroxytryptamine type 3 (5-HT3) receptor is a member of the Cys-loop superfamily of ligand-gated ion channels (LGICs) and modulated by pharmacologic relevant concentrations of volatile anesthetics or n-alcohols like most receptors of LGICs. The goal of this study was to reveal whether the site-directed single mutations of E-106, F-107 and R-222 in 5-HT3 receptor may affect the anesthetic modulation of halothane known as positive modulator. Methods: The wild-type and mutant receptors, E106D, F107Y, R222F, R222V, were expressed in Xenopus Laevis oocytes and receptor function was assessed using two electrode voltage clamp techniques. Results: E106D, F107Y, R222F, R222V mutant 5-HT3A receptors were functionally expressed. F107Y mutant 5-HT3A receptors displayed decreased sensitivity to 5-HT compared to the wild type 5-HT3A receptor (P<0.05). Halothane showed positive modulation in both wild and F107Y mutant 5-HT3A receptors but F107Y mutant 5-HT3 receptor showed greater enhancing modulation comparing to wild-type receptor. Meanwhile, R222F and R222V mutant 5-HT3 receptor lost positive modulation with 1 and 2 MAC of halothane. Most interestingly, positive modulation by halothane was converted into negative modulation in E106D mutant 5-HT3A receptor. Conclusions: The present study implicate the amino acid residues known for agonist binding and linking agonist binding to channel gating might also have important role for anesthetic modulation in 5-HT3A receptor. (Korean J Anesthesiol 2009;56:66~73)

Fentanyl 을 이용한 정주용 자가통증조절시 저용량 Naloxone 의 효과에 대한 비교 연구

김미경,길혜금,김원옥,구본녀 대한마취과학회 2001 Korean Journal of Anesthesiology Vol.41 No.2

The Effects of Low-dose Naloxone in Intravenous Fentanyl Patient-Controlled Analgesia Bon Nyeo Koo, M.D., Hae Keum Kil, M.D., Won Oak Kim, M.D. and Mi Kyeong Kim, M.D. Department of Anesthesiology, Yonsei University College of Medicine, Seoul, Korea Background: The use of a low-dose naloxone infusion concomitant with intravenous morphine PCA(patient-controlled analgesia) attenuates opioid-related side effects without reducing analgesic effects. The authors compared the incidence of morphine-related side effects and the quality of analgesia in adding low-dose naloxone, normal saline or droperidol to an IV fentanyl PCA regimen. Methods: One hundred eight patients undergoing ocular plastic surgery were enrolled in the study. General anesthesia was induced and maintaine with propofol TCI (target controlled infusion), vecuronium or pancuronium and nitrous oxide. After intubated, they received intravenous fentanyl as PCA. They were randomized to receive normal saline, droperidol of low-dose naloxone concomitant with IV fentanyl PCA. Verbal rating scores for pain, the degree of patients` satisfaction (1~4), nausea, vomiting, requests for antiemetics, urinary retention, pruritus and respiratory depression were recorded after 24 hours. Results: There was no difference in the VRS (verbal rating score) for pain, degree of the satisfaction and the incidence of nausea, vomiting and requests of antiemetics among the three groups. There was no incidence of pruritus or respiratory depression. One subject developed urinary retention in the control group, and three cases in the droperidol group, but none was developed in the low-dose naloxone group. Conclusions: There was no difference in the prevention of postoperative nausea, or vomiting among the normal saline, droperido., and naloxone groups with an IV fentanyl PCA. Low-dose naloxone, however, had a reducing effect on urinary retention; it may become an alternative choice according to the anesthesiologist`s preference. (Korean J Anesthesiol 2001; 41: 190~194)