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Antioxidant and Immunomodulatory Activities of Polysaccharides from Moxa (Artemisia argyi) Leaf
Min-Bo Lan,Yan-Hong Zhang,Ying Zheng,Hui-Hui Yuan,Hong-Li Zhao,Feng Gao 한국식품과학회 2010 Food Science and Biotechnology Vol.19 No.6
The purpose of the current study was to characterize the polysaccharides isolated from the moxa (Artemisia argyi) leaf and investigate their bioactivities. The molecular weights of the purified polysaccharides were at a range from 6,000 to 10,000 Da and consisted mostly of carbohydrates (>90%), mainly the monosaccharide of xylose, arabinose, mannose, and glucose. The isolated polysaccharides were further fractionated to form 4subfractions and all of the subfractions exhibited high antioxidant capability on both hydroxide (IC50 ranging from 8 to 18 μg/mL) and superoxide anion radicals (IC50ranging from 10 to 30 μg/mL). The moxa leaf polysaccharides were also capable of enhancing the Concanavallin A (ConA)-induced T cell proliferation, but not the lipopolysaccharides (LPS)-induced B cell proliferation in the in vitro immunological tests. Furthermore, the polysaccharides also strongly facilitate ConA-induced secretion of interferon-γ (IFN-γ) and interleukin-2 (IL-2) in a dose-dependent manner. The results suggested that the moxa leaf polysaccharides may have potential applications as antioxidants and immune enhancers.
Lan, Nguyen Thi,Kim, Hyoung Jin,Han, Hyun-Ja,Lee, Deok-Chan,Kang, Bo Kyu,Han, Sang Yoon,Moon, Hyoungjoon,Kim, Hong-Jin Elsevier 2018 Biologicals Vol.51 No.-
<P><B>Abstract</B></P> <P>Virus-like particles (VLPs) are multi protein complexes mimicking the structural properties of the native virus. The development of freeze-dried formulations of such complex protein structures remains a challenge. Red-spotted grouper nervous necrosis virus (RGNNV) causes mass mortality in fish culture, and RGNNV VLPs have been suggested to be promising vaccine candidates. In the present study, the stability of RGNNV VLPs in the liquid state was investigated over a 4-week period, along with the influence of freeze-drying on VLP stability. RGNNV VLPs were completely degraded after one week at 37 °C followed by 3 weeks at ambient temperature, and they were partially degraded after 4 weeks at 4 °C. Therefore, the inherent stability of RGNNV VLP in an aqueous milieu is insufficient for long-term storage. When RGNNV VLPs were freeze-dried in the presence or absence of sugar stabilizers, sorbitol was found to improve VLP stability whereas mannitol reduced it. VLP preparations freeze-dried with sorbitol or without stabilizer were as immunogenic as control (non-freeze dried) VLPs, whereas VLPs freeze-dried in mannitol were less immunogenic. These results indicate that freeze-dried RGNNV VLPs have potential as vaccines.</P>
Characterization and differentiation ability of mesenchymal stem cell in the canine endometrium
Bo Myeong Lee,Eun Young Kim,Lili Zhuang,Dong Eon Kim,Chi Sun Yun,Eun Ji Lee,Ju Lan Chun,Min Kyu Kim 한국수정란이식학회 2017 한국수정란이식학회 학술대회 Vol.2017 No.05
The mesenchymal stem cells (MSC) has been investigated as a source of stem cell therapy to replace and treat damaged cells. Human endometrial epithelial and stromal cells was isolated from hysterectomy tissue and the direct evidence of stem/progenitor cells in the human endometrium was identified. Endometrium derived stem cells (EnMSCs) are known to have a high proliferative ability, genetic stability, lack of tumorigenicity and low immnunogenicity during long-term cultivation. Here, we aimed to identify MSC in canine endometrium and characterize its potential to differentiate into decidua cells. EnMSCs were isolated from thrown-away spayed uterus of adult canine depending on their estrus cycle, and identified by flow cytometry, immunocytochemistry and flow cytometry with MSC specific markers. We then characterized the ability of EnMSCs by the doubling-time analysis, colony-forming units and MSC differentiation assays. Isolated EnMSCs expressed stem cell specific genes (Sox2, Oct4, Nanog, MCAM, Endoglin, Susd2 and IGTB) and MSC surface markers (CD90, CD44 and CD117). EnMSCs are also differentiated into adipogenic, osteogenic and chondrogenic cells morphologically under modified conditions with the expression of lineage specific genetic markers. EnMSCs showed higher proliferation ability than canine amniotic fluid derived MSCs which were used as a positive control. EnMSCs were cultured at low density (10, 20, cells/cm2) and initiated to form small colonies of loosely-arranged cells and gradually formed large colonies of densely-packed cells which underwent self-renewal with high proliferative potential which is similar to the clonogenicity feature of human endometrium-derived stem cells. EnMSCs were then induced to differentiate into decidua cells with 0.5 mM dbcAMP. After 14 days, EnMSCs changed their morphology into the elongated and rounded shape. The induced decidual cells expressed PRL and IGFBP1 which are typically expressed in decidua cells. In conclusion, we successfully isolated and characterized MSC in the canine endometrium which differentiated into decidua cells. These results showed that endometrium may be a promising source of stem cells, and furthermore raise the possibility of canine EnMSCs as a novel hypothetical decidualisation model of infertility associated with decidualisation insufficiency and implantation failure.
The microRNA expression profiles of mouse mesenchymal stem cell during chondrogenic differentiation
( Bo Yang ),( Hong Feng Guo ),( Yu Lan Zhang ),( Shi Wu Dong ),( Da Jun Ying ) 생화학분자생물학회(구 한국생화학분자생물학회) 2011 BMB Reports Vol.44 No.1
MicroRNAs are potential key regulators in mesenchymal stem cells chondrogenic differentiation. However, there were few reports about the accurate effects of miRNAs on chondrogenic differentiation. To investigate the mechanisms of miRNAs-mediated regulation during the process, we performed miRNAs microarray in MSCs at four different stages of TGF-β3-induced chondrogenic differentiation. We observed that eight miRNAs were significantly up-regulated and five miRNAs were down- regulated. Interestingly, we found two miRNAs clusters, miR-143/145 and miR-132/212, kept on down-regulation in the process. Using bioinformatics approaches, we analyzed the target genes of these differentially expressed miRNAs and found a series of them correlated with the process of chondrogenesis. Furthermore, the qPCR results showed that the up-regulated (or down-regulated) expression of miRNAs were inversely associated with the expression of predicted target genes. Our results first revealed the expression profiles of miRNAs in chondrogenic differentiation of MSCs and provided a new insight on complicated regulation mechanisms of chondrogenesis. [BMB reports 2011; 44(1): 28-33]
INTEGRATING MODEL CHECKING INTO TEST-DRIVEN DEVELOPMENT IN SOFTWARE DEVELOPMENT
Bo Su,Lan Shen 한국멀티미디어학회 2006 한국멀티미디어학회 국제학술대회 Vol.2006 No.-
The purpose of this paper is to discuss integrating agile methods and formal methods into a new lightweight method. The benefits and shortcomings of both methods are analyzed. Stratagems of integrating are analyzed. Since Test-Driven Development is a typical approach of agile methods and model checking is an important approach in formal methods, a new approach of integrating test-driven development and model checking is provided. The result of a case study shows that the new approach is possible and has the virtues of both approaches.
Bo Wu,Ming Lu,Wei Huang,Yangbing Lan,Yufang Wu,Zonghui Huang 대한토목학회 2020 KSCE JOURNAL OF CIVIL ENGINEERING Vol.24 No.11
Because of the harsh environment around city subways and the typically poor geological conditions, it is often difficult to ensure the stability of a tunnel, which makes it prone to collapses and other accidents. The influence of multiple factors on tunnel stability was analyzed under the same geological conditions and environmental factors by using a three-dimensional numerical simulation. Taking into account the objective environment and manual operation and other factors, construction sequence, support closure time, and reinforcement area and parameters were chosen as the main factors affecting the tunnel stability. Finally, based on ground deformation, tunnel convergence deformation, and surrounding soil stress as evaluation indicators, a fuzzy analytic hierarchy process (FAHP)-gray correlation degree-technique for order performance by similarity to ideal solution (TOPSIS) model was established. It was found that the higher the relative closeness of the construction parameter combination, the greater the tunnel stability. The best combination of construction parameters was applied to the Tiantongyuan Station project of Beijing Metro Line 17. All geological conditions being equal, reasonable selection of construction parameters can ensure optimal tunnel construction stability, reduce construction risks, and support sustainable tunnel development. In addition, the FAHP-gray correlation degree-TOPSIS method achieved multi-index evaluation of tunnel stability, which provides practical guidance for the construction of similar projects.
Lan-Qing Ma,Yan-Wu Guo,Hui-Li Guo,Xing Li,Li-Li Huang,Bo-Ning Zhang,Xiao-Bin Pang,Ben-Ye Liu,Hong Wang 한국식물생명공학회 2013 Plant biotechnology reports Vol.7 No.3
In our recent work (Ma et al., in Planta229(3):457–469, 2009a and 229(4):1077–1086, 2009b),two three-intron type III PKS genes, PcPKS1 and PcPKS2,were isolated from Polygonum cuspidatum Sieb. et Zucc. Phylogenetic and functional analyses revealed PcPKS1 is athree-intron chalcone synthase (CHS) gene, and PcPKS2 isfound to be a three-intron benzalacetone synthase (BAS)gene. The regular CHS encoded by a single intron genehave not been isolated and characterized from P. cuspidatum. In this work a further CHS with one intron (PcPKS3)and a stilbene synthase (STS) gene with three-intron(PcPKS5) were isolated and characterized by functionaland phylogenetic analyses. In comparison with PcPKS1, abifunctional enzyme with both CHS and BAS activity, theenzymatic product of recombinant PcPKS3 was naringenin,bis-noryangonin (BNY) and 4-coumaroyltriacetic acidlactone (CTAL) occurred as side products. The PcPKS5synthesized resveratrol and a trace amount of naringeninfrom p-coumaroyl-CoA. To our knowledge, PcPKS5 is thefirst reported three-intron STS gene in flowering plants. Inthis work, we speculated that this involved a possibleevolutionary route of plant-specific type III PKS superfamilyin P. cuspidatum.