Grafting improves salinity tolerance of bell pepper plants during greenhouse production

Blanca E. Orosco-Alcalá,Héctor G. Núñez-Palenius,Fidel Díaz-Serrano,Luis Pérez-Moreno,Mauricio Valencia-Posadas,Libia I. Trejo-Tellez,Nicacio Cruz-Huerta,Juan I. Valiente-Banuet 한국원예학회 2021 Horticulture, Environment, and Biotechnology Vol.62 No.6

Biotic and abiotic stresses aff ect plant growth and productivity. High-salinity stress aff ects crop yield, causing fi nancialloss to growers. The use of salt-tolerant rootstocks is a strategy that has been used to reduce salt damage in crops. A studywas conducted to evaluate the eff ect of salinity-resistant rootstocks on the physiological and morphological characteristicsof bell pepper plants grown under greenhouse conditions. A factorial experiment was conducted using a completely randomizeddesign with two factors. The bell pepper ‘Viper’ cultivar was grafted on two reported salinity-tolerant rootstocks(E21R10144 and E21R10197), and non-grafted (NG) plants were used as the control. Four salinity levels were applied tothe plants (electrical conductivity treatments using NaCl of 2, 4, 6, and 8 dS m −1 ) in Steiner nutritive solution (100%). Theresponse to salinity was determined using morphological and physiological plant parameters, including fruit yield. Increasedtolerance to salinity conditions (NaCl) was observed in the ‘Viper’ bell pepper grafted on E21R10144, which allowed greaterplant height, stem diameter, leaf size, as well as fresh and dry biomass of both the roots and canopy. The impacts on plantphysiological response, including photosynthesis, stomatal conductance, transpiration, water content, stomatal density, andfoliar area, were also determined. Our results indicate that the use of the salinity-tolerant bell pepper rootstock E21R10144maintained plant homeostasis and minimized the damage caused by salts to the morphology and physiology, as well as eff ectson fruit yield; thus, it is a promising tool for the management of salt stress.

Statistical Study and Prediction of Variability of Erythemal Ultraviolet Irradiance Solar Values in Valencia, Spain

Gonzalo Gurrea,Vicente Blanca-Giménez,Vicente Pérez,María-Antonia Serrano,Juan-Carlos Moreno,Solar Radiation Group of the Universitat Politècnica de València 한국기상학회 2018 Asia-Pacific Journal of Atmospheric Sciences Vol.54 No.4

The goal of this study was to statistically analyse the variability of global irradiance and ultraviolet erythemal (UVER) irradiance and their interrelationships with global and UVER irradiance, global clearness indices and ozone. A prediction of short-term UVER solar irradiance values was also obtained. Extreme values of UVER irradiance were included in the data set, as well as a time series of ultraviolet irradiance variability (UIV). The study period was from 2005 to 2014 and approximately 250,000 readings were taken at 5-min intervals. The effect of the clearness indices on global irradiance variability (GIV) and UIV was also recorded and bi-dimensional distributions were used to gather information on the two measured variables. With regard to daily GIVand UIV, it is also shown that for global clearness index (kt) values lower than 0.6 both global and UVER irradiance had greater variability and that UIVon cloud-free days (kt higher than 0.65) exceeds GIV. To study the dependence between UIVand GIV the χ2 statistical method was used. It can be concluded that there is a 95% probability of a clear dependency between the variabilities.A connection between high kt (corresponding to cloudless days) and low variabilities was found in the analysis of bidimensional distributions. Extreme values of UVER irradiance were also analyzed and it was possible to calculate the probable future values of UVER irradiance by extrapolating the values of the adjustment curve obtained from the Gumbel distribution.

Skin Testing With Peach Peel Extract Versus Serum IgE to Pru p 3 as a Stronger Predictor of Peach-Induced Anaphylaxis

Somoza Maria Luisa,Prieto-Moreno Pfeifer Ana,Martín-Pedraza Laura,Victorio Puche Laura,Esteban Rodríguez Angel,Blanca-López Natalia,Eva Fernández González Abel,Fernández-Caldas Enrique,Morán Morales M 대한천식알레르기학회 2021 Allergy, Asthma & Immunology Research Vol.13 No.6

The most important peach fruit allergen is Pru p 3, followed by Pru p 1, Pru p 4, and Pru p 7. We aimed to assess their role in subjects with peach fruit-induced allergy (anaphylaxis and OAS) and compare skin prick tests (SPT) vs. specific immunoglobulin E (sIgE) for predicting anaphylaxis. We also selected a control group. SPT included prevalent inhalant and plant food allergens plus peach peel extract. The sIgE to Pru p 1, Pru p 3, Pru p 4, and Pru p 7 were quantified. Compared with controls (n = 42), cases (n = 41) were younger (P = 0.003), more frequently female (P < 0.05) and had higher SPT positivity to peach peel (44% vs. 2.4%, P < 0.0001). There were significant differences in sensitization to several pollens: Olea europaea, Artemisia vulgaris, Prunus persica, Platanus acerifolia (all P < 0.001); and fruits: apple (P < 0.04), peanut (P < 0.002), tomato (P < 0.005), and melon (P < 0.05). Pru p 3 sIgE was detected in 61% of all cases (85% anaphylaxis and 38% OAS; P < 0.01 each) and 5% of controls (P < 0.001). Pru p 4 sIgE was present in 19% of cases and 7% of controls. The sIgE to Pru p 1 and Pru p 7 were not found. The odds ratio to predict anaphylaxis for peach peel SPT was 113 (confidence interval [CI], 20–613; P < 0.0001); for sIgE to Pru p 3, 22 (CI, 5.3–93; P < 0.0001); and for SPT positivity to selected plant food allergens, 5 (CI, 1–19; P < 0.05). In our study group, SPT with peel peach extract was a better predictor of anaphylaxis than Pru p 3 sIgE or other variables considered. The role of sIgE to Pru p 1, Pru p 4, and Pru p 7 seemed negligible.

Clavulanic Acid Is a Leading Culprit Beta-Lactam in Immediate Allergic Reactions to Penicillins

Torres-Rojas Isabel,Pérez-Alzate Diana,Somoza Maria Luisa,Pfeifer Ana Prieto-Moreno,Diaz Elisa Haroun,Jimenez-Rodriguez Teodorikez W,Sánchez Javier Fernández,Ruano Francisco J,Blanca Miguel,Blanca-Lóp 대한천식알레르기학회 2023 Allergy, Asthma & Immunology Research Vol.15 No.2

Purpose: Clavulanate, a beta-lactam associated with amoxicillin, is frequently prescribed in patients at all ages. Recent data implicate amoxicillin-clavulanate in up to 80% of beta-lactam allergy cases. We assessed clavulanate’s role in inducing allergic reactions to this combination treatment, with a focus on selective immediate reactions. Methods: Adults (≥ 16 years) reporting a history of immediate reactions to amoxicillin-clavulanate were evaluated through a beta-lactam allergological workup, using modified European Academy of Allergy and Clinical Immunology guidelines. Patients first underwent skin testing, and if negative, drug provocation tests. Expected outcomes were: Group A, subjects with immediate reaction to classical penicillin group determinants (penicilloyl polylysine, minor determinants mixture, and/or penicillin G); Group B, subjects with selective immediate reaction to amoxicillin; Group C, subjects with selective immediate reaction to clavulanate and Group D, those immediate reactions with co-sensitization to clavulanate plus penicillin group determinants or amoxicillin. Results: Of 1,170 included patients, 104 had immediate reactions: 36.5% to penicillin group determinants (Group A), 26.9% to amoxicillin (Group B), 32.7% to clavulanate (Group C), and 3.8% to clavulanate plus penicillin determinants or amoxicillin (Group D). Diagnosis was made by skin testing in 79%, 75% and 47% of the patients, respectively, in the first 3 groups (P < 0.001). Drug provocation tests were necessary to establish most other diagnoses. Anaphylaxis predominated over urticaria/angioedema in all groups. Conclusions: Selective immediate reactions to clavulanate accounted for over a third of cases with confirmed reactions after amoxicillin-clavulanate intake, with more than half experiencing anaphylaxis. Within this group, skin test sensitivity was below 50%. People taking amoxicillin-clavulanate may also be co-sensitized to both drugs.

Genetic variation of Colletotrichum magnum isolated from Carica papaya as revealed by DNA fingerprinting

Daisy Pérez-Brito,Alberto Cortes-Velázquez,Teresita Valencia-Yah,Anuar Magaña-Álvarez,Cuauhtémoc Navarro,Blanca Moreno,Steven Quiroga,Raúl Tapia-Tussell 한국미생물학회 2018 The journal of microbiology Vol.56 No.11

Mexico is one of the five largest producers of papaya worldwide, but losses caused by pathogens, mainly fungus, at the pre- and post-harvest stages are often more than 50% of the crop. Papaya anthracnose, caused by three different species of the Colletotrichum genus in Mexico, occupies a preponderant place in this problem. Although two of these species, C. gloeosporiodes and C. truncatum, have been characterized morphologically and genotypically, this has not occurred with C. magnum, the third species involved, about which there is very little information. Because of this, it is vital to know its genetic characterization, much more so considering that the studies carried out on the other two species reveal a wide genetic diversity, differences in pathogenicity and in the response to fungicides of the different strains characterized. In this work, Colletotrichum spp. isolates were collected at different papaya orchards in the south-southeast of Mexico. C. magnum isolates identified by species-specific primers were characterized by morphological and molecular approaches. Differences in colony characteristics resulted in five morphological groups. AP-PCR, DAMD and ISSR markers were found to be very efficient for revealing the interspecific variability of this species. The high genetic variability found in the accessions of C. magnum was linked to the geographical area where they were collected. Isolates from Chiapas State were the most variable, showing point mutations in the ITS1- ITS2 region. These results will enable a better phytosanitary management of anthracnose in papaya in this region of Mexico.