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( Azam Rahimpour ),( Behrouz Vaziri ),( Reza Moazzami ),( Leila Nematollahi ),( Farzaneh Barkhordari ),( Leila Kokabee ),( Ahmad Adeli ),( Fereidoun Mahboudi ) 한국미생물 · 생명공학회 2013 Journal of microbiology and biotechnology Vol.23 No.8
Cell line development is the most critical and also the most time-consuming step in the production of recombinant therapeutic proteins. In this regard, a variety of vector and cell engineering strategies have been developed for generating high-producing mammalian cells; however, the cell line engineering approach seems to show various results on different recombinant protein producer cells. In order to improve the secretory capacity of a recombinant tissue plasminogen activator (t-PA)-producing Chinese hamster ovary (CHO) cell line, we developed cell line engineering approaches based on the ceramide transfer protein (CERT) and X-box binding protein 1 (XBP1) genes. For this purpose, CERT S132A, a mutant form of CERT that is resistant to phosphorylation, and XBP1s were overexpressed in a recombinant t-PA-producing CHO cell line. Overexpression of CERT S132A increased the specific productivity of t-PA-producing CHO cells up to 35%. In contrast, the heterologous expression of XBP1s did not affect the t-PA expression rate. Our results suggest that CERTS132A- based secretion engineering could be an effective strategy for enhancing recombinant t- PA production in CHO cells.
Removal of azo dye from aqueous solution using an anionic polymeric urethane absorbent (APUA)
Azam Pirkarami,Mohammad Ebrahim Olya,Farhood Najafi 한국공업화학회 2015 Journal of Industrial and Engineering Chemistry Vol.21 No.1
This paper is a report on an original research which investigated the effect of a number of experimentalparameters on the removal of Food Red 17 (FR17) from an aqueous solution using anionic polymericurethane absorbent (APUA) as an adsorbent. The optimum value of adsorbent dose was found to be35 mg L 1. Further, maximum dye removal took place at pH 3 and 45 8C. The fourth parameter, stirringthe solution during the treatment, also resulted in significant removal improvement. The amount of FR17adsorbed on APUA surface was quantified using the Langmuir equation. UV irradiation was also found tohave a positive effect on the removal process. The efficiency of the treatment was verified using FT-IRspectrometry results for APUA, FR17, and APUA-FR17. Finally, the adsorbent was subjected to SEMcharacterization.
An Improved Linear Complementarity Solver for the Dynamic Analysis of Blast Loaded Structures
Azam Khan,Moiz Tariq,Asad Ullah,Arshad Hussain 한국콘크리트학회 2022 International Journal of Concrete Structures and M Vol.16 No.5
The linear complementarity problem (LCP) approach, expedited by using the simple rigid–plastic theory, has been utilized successfully in predicting the numerical response of the ductile steel or concrete structures subjected to short-duration, high-intensity dynamic loads. The current study attempts to improve the computational stability of this powerful technique while determining the response of skeletal structures under blast loading. The performance of the Lemke LCP solver is amplified by introducing an automatic time-stepping scheme to efficiently trace the complex dynamic response using either lumped mass or continuous mass discretization. The computational efficiency of this solver is tested against carefully chosen three numerical examples, and the acquired results are in good agreement with the derived closed-form solution and results from other sources.
Azam Chahardoli,Farshad Qalekhani,Yalda Shokoohinia,Ali Fattahi 한국공업화학회 2022 Journal of Industrial and Engineering Chemistry Vol.111 No.-
Titanium dioxide nanoparticles (TiO2NPs) were produced using a crud flavonoid luteolin (LT) by the greenprocess. Physicochemical characterizations of LT mediated synthesis of TiO2NPs (LT-TiO2NPs) were analyzedby different techniques. The biocompatibility of LT-TiO2NPs was evaluated by assessing their cytotoxicity,inhibition of protein denaturation, hemolysis effects, and membrane stabilization activity. Thesynthesized LT-TiO2NPs exhibited mostly rod-shaped morphology, rutile crystalline structure with lengthabout 33.3–135 nm and width in 16.6–58 nm. Under LT-TiO2NPs treatments, the cell viability of cancercells was decreased in a dose-dependent manner. According to the results, the IC50 values of LT-TiO2NPswere between 12.5–25 lg/mL for A375 and MCF-7 cancer cells, and its value was below 50 lg/mL for skinfibroblast. These NPs also induced intracellular reactive oxygen species (ROS) generation and causedmitochondrial disruption in cancer cells, leading to the induction of intrinsic apoptosis. These biosynthesizedNPs did not show denaturation of bovine serum albumin (BSA) protein and hemolytic or heatinducedhemolytic effects, which confirmed their membrane stabilization activity without damagingred blood cells (RBCs) membrane integrity. Therefore, this research provides valuable results in the caseof green synthesis of LT-TiO2NPs and their specific properties, biocompatibility, and their potential to beused in biomedical fields.
New Findings on Breast Cancer Stem Cells: A Review
Azam Bozorgi,Mozafar Khazaei,Mohammad Rasool Khazaei 한국유방암학회 2015 Journal of breast cancer Vol.18 No.4
Since the introduction of the “cancer stem cell” theory, significant developments have been made in the understanding of cancer and the heterogenic structure of tumors. In 2003, with the isolation of cancer stem cells from the first solid tumor, breast cancer, and recognition of the tumorigenicity of these cells, this theory suggested that the main reason for therapy failure might be the presence of cancer stem cells. This review article describes breast cancer stem cell origin, the related cellular and molecular characteristics, signaling pathways, and therapy resistance mechanisms. The databases PubMed, SCOPUS, and Embase were explored, and articles published on these topics between 1992 and 2015 were investigated. It appears that this small subpopulation of cells, with the capacity for self-renewal and a high proliferation rate, originate from normal stem cells, are identified by specific markers such as CD44+/CD24-/low, and enhance a tumor’s capacity for metastasis, invasion, and therapy resistance. Cancer stem cell characteristics depend on their interactions with their microenvironment as well as on the inducing factors and elements. Although uncertainties about breast cancer stem cells exist, many of researchers believe that cancer stem cells should be considered as possible therapeutic targets.
Role of interleukin-6 (IL-6) in predicting gestational diabetes mellitus
( Azam Amirian ),( Mahin Balouchi Mahani ),( Fatemeh Abdi ) 대한산부인과학회 2020 Obstetrics & Gynecology Science Vol.63 No.4
Gestational diabetes mellitus (GDM) is the most common pregnancy-associated metabolic disorder that is steadily increasing worldwide. Early diagnosis of pregnant women susceptible to GDM is the first step for deploying effective preventive treatment to reduce maternal, fetal, and neonatal complications. The diagnostic process of GDM is still controversial and interleukin-6 (IL-6) is one of the most recent markers used for the diagnosis of GDM. In this study, we aimed to systematically review the role of IL-6 in the diagnosis of GDM. In this systematic review, Google Scholar, Scopus, PubMed, ISI Web of Science, ProQuest, and MEDLINE databases were searched using the following keywords: GDM, screening, and IL-6, with the time interval 2009-2020. The quality of articles was assessed using the Strengthening the Reporting of Observational Studies in Epidemiology checklist. Twenty-four articles with desired quality that met the inclusion criteria were selected and reviewed further. Sixteen studies showed a statistically significant association, while 8 studies did not report any relationship between IL-6 levels and GDM. Based on the results of these studies, assessing the serum IL-6 levels can be investigated a newly established diagnostic biomarker for GDM. Therefore, through early diagnosis of susceptible women, effective measures can be implemented to reduce its complications.
Individual Fit Testing of Hearing Protection Devices Based on Microphone in Real Ear
Azam Biaban,Mohsen Aliabadi,Rostam Golmohammadi,Maryam Farhadian 한국산업안전보건공단 산업안전보건연구원 2017 Safety and health at work Vol.8 No.4
Labeled noise reduction (NR) data presented by manufacturers are considered one of the main challenging issues for occupational experts in employing hearing protection devices (HPDs). This study aimed to determine the actual NR data of typical HPDs using the objective fit testing method with a microphone in real ear (MIRE) method. Five available commercially earmuff protectors were investigated in 30 workers exposed to reference noise source according to the standard method, ISO 11904-1. Personal attenuation rating (PAR) of the earmuffs was measured based on the MIRE method using a noise dosimeter (SVANTEK, model SV 102). The results showed that means of PAR of the earmuffs are from 49% to 86% of the nominal NR rating. The PAR values of earmuffs when a typical eyewear was worn differed statistically (p < 0.05). It is revealed that a typical safety eyewear can reduce the mean of the PAR value by approximately 2.5?dB. The results also showed that measurements based on the MIRE method resulted in low variability. The variability in NR values between individuals, within individuals, and within earmuffs was not the statistically significant (p > 0.05). This study could provide local individual fit data. Ergonomic aspects of the earmuffs and different levels of users experience and awareness can be considered the main factors affecting individual fitting compared with the laboratory condition for acquiring the labeled NR data. Based on the obtained fit testing results, the field application of MIRE can be employed for complementary studies in real workstations while workers perform their regular work duties.
A Control Chart for Monitoring the Process Mean Using Successive Sampling Over Two Occasions
Azam, M.,Arshad, A.,Aslam, M.,Jun, C. H. Springer Science + Business Media 2017 Arabian journal for science and engineering: AJSE Vol.42 No.7
<P>This article proposes a new control chart to monitor the process mean based on the sample statistics from two successive occasions. First occasion units are independently drawn to obtain information of the study variable, and the second occasion units are drawn collectively with a retained portion of first occasion as matched units, while the other portion is the collection of the fresh units from the remaining unselected units at first occasion. The in-control and out-of-control average run lengths (ARLs) are derived for performance assessment under mean shifts. The performance of the proposed control chart is compared with traditional Shewhart control chart and a EWMA chart in terms of ARLs, which exhibits better performance when process is shifted from the target mean.</P>