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Postthrombolysis hemorrhage risk is affected by stroke assessment bias between hemispheres
Audebert, H. J.,Singer, O. C.,Gotzler, B.,Vatankhah, B.,Boy, S.,Fiehler, J.,Lansberg, M. G.,Albers, G. W.,Kastrup, A.,Rovira, A.,Gass, A.,Rosso, C.,Derex, L.,Kim, J. S.,Heuschmann, P. Ovid Technologies (Wolters Kluwer) - American Acad 2011 Clinical Neurophysiology Vol.76 No.7
Kim, Yuna,Kim, Eunkyoung,Clavier, Gilles,Audebert, Pierre Royal Society of Chemistry 2006 Chemical communications Vol.2006 No.34
<P>A new electrofluorescent switch was prepared with an electroactive fluorescent tetrazine blend of polymer electrolyte; the cells contain four layers: the tetrazine polymer film, a photocured polymer electrolyte film, and two indium-tin oxide plates as the two contact electrodes.</P> <P>Graphic Abstract</P><P>A new electrofluorescent switch was prepared with an electroactive fluorescent tetrazine blend of polymer electrolyte. <IMG SRC='http://pubs.rsc.org/services/images/RSCpubs.ePlatform.Service.FreeContent.ImageService.svc/ImageService/image/GA?id=b608312a'> </P>
White Electrofluorescence Switching from Electrochemically Convertible Yellow Fluorescent Dyad
Seo, Seogjae,Kim, Yuna,Zhou, Qing,Clavier, Gilles,Audebert, Pierre,Kim, Eunkyoung WILEY‐VCH Verlag 2012 Advanced Functional Materials Vol.22 No.17
<P><B>Abstract</B></P><P>A fluorescent naphthalimide‐tetrazine dyad (NITZ) was examined for electrofluorochromism. The reversible electrochemistry of the tetrazine was accompanied by the fluorescence change through a quasi‐complete energy transfer in an electrochemical cell prepared by the mixture of polymer electrolyte and naphthalimide‐tetrazine dyad. Owing to the energy transfer within the dyad (naphthalimide and tetrazine), the fluorescence efficiency of NITZ was much enhanced and the effective fluorophore concentration in this system was much less than other tetrazine based electrofluorochromic device (EFD). Thus the yellow fluorescence of NITZ was switched on and off remarkably even with small quantity of NITZ (1 wt.%) in an EFD upon application of step potentials for different redox state. Furthermore, multi‐color fluorescence switching was achieved by blending a naphthalimide to the electrofluorochromic layer, to show white‐blue‐dark state of fluorescence. Since the tetrazine and naphthalimide units have their emission quenched at different potentials, the emission color could be tuned by quenching emission at selected wavelengths, reversibly, under low working potentials.</P>
Prebet, Thomas,Lhoumeau, Anne-Catherine,Arnoulet, Christine,Aulas, Anaï,s,Marchetto, Sylvie,Audebert, Sté,phane,Puppo, Francesca,Chabannon, Christian,Sainty, Danielle,Santoni, Marie-Jos&eacu American Society of Hematology 2010 Blood Vol.116 No.13
<B>Abstract</B><P>The pseudo tyrosine kinase receptor 7 (PTK7) is an orphan tyrosine kinase receptor assigned to the planar cell polarity pathway. It plays a major role during embryogenesis and epithelial tissue organization. Here we found that PTK7 is also expressed in normal myeloid progenitors and CD34+ CD38− bone marrow cells in humans. We performed an immunophenotyping screen on more than 300 patients treated for hematologic malignancies. We demonstrated that PTK7 is expressed in acute myeloid leukemia (AML) and is mostly assigned to granulocytic lineage differentiation. Patients with PTK7-positive AML are more resistant to anthracycline-based frontline therapy with a significantly reduced leukemia-free survival in a multivariate analysis model. In vitro, expression of PTK7 in cultured leukemia cells promotes cell migration, cell survival, and resistance to anthracycline-induced apoptosis. The intracellular region of PTK7 is required for these effects. Furthermore, we efficiently sensitized primary AML blasts to anthracycline-mediated cell death using a recombinant soluble PTK7-Fc protein. We conclude that PTK7 is a planar cell polarity component expressed in the myeloid progenitor compartment that conveys promigratory and antiapoptotic signals into the cell and that represents an independent prognosis factor of survival in patients treated with induction chemotherapy.</P>
Electrofluorescence switching of tetrazine-modified TiO2 nanoparticles.
Seo, Seogjae,Allain, Cl?mence,Na, Jongbeom,Kim, Sehwan,Yang, Xu,Park, Chihyun,Malinge, J?r?my,Audebert, Pierre,Kim, Eunkyoung RSC Pub 2013 Nanoscale Vol.5 No.16
<P>Highly fluorescent tetrazine-modified TiO2 nanoparticles were prepared by the reaction of triethoxysilane-appended chloroalkoxy tetrazine (ESTZ) with TiO2 nanoparticles through a condensation reaction between the surface hydroxyl groups of an electrode and the silane anchor group of ESTZ. The prepared electrodes were used as robust fluorescent layers for electrochemical fluorescence switching (electrofluorochromism) applications in the presence of 2,2,6,6-tetramethyl-1-piperidinyloxy (TEMPO) as a charge balancing mediator. The stable charge balancing mediator, TEMPO, in the electrolyte was found to be essential to reduce the intrinsic electron transport resistance of TiO2 in order to achieve reversible electrofluorescence switching. Furthermore it facilitated a fully reversible electrochemical reaction and provided a sufficient charge balance, which allowed us to realize semiconductor-based electrofluorescence switching with an on/off ratio of 4.0 and cyclability greater than 100 cycles.</P>
Nikolaos Tsafantakis,Efrosini S. Katsanou,Katerina Kyriakopoulou,Eirini-Christina Psarou,Iliana Raptaki,Alexios L. Skaltsounis,Marc Audebert,Kyriaki A. Machera,Nikolas Fokialakis 한국식품영양과학회 2019 Journal of medicinal food Vol.22 No.12
Opuntia ficus indica has been an important dietary source and a traditionally used medicinal plant. Given the promising health-promoting properties of this plant, a comparative toxicological assessment and antioxidant bioevaluation of extracts from different parts of the plant were carried out in relation to their chemical profile. Toxicity was examined at multiple endpoints using the 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide (MTT), Comet and the γH2AX In-Cell Western Assay, while hyphenated ultra-high-performance liquid chromatography-high-resolution mass spectrometry (UHPLC-HRMS) analysis was carried out to identify main constituents. None of the extracts showed any cytotoxic and genotoxic effect on cell lines used, apart from the flower extract in HepG2 cells at the highest concentration tested (2.5 mg/mL). Both fruit flesh and seed extracts demonstrated a prominent protective effect against H2O2-induced genotoxicity in almost all concentrations tested, while extracts originated from flowers and cladodes were effective only at the low non-cytotoxic (0.312 and 0.625 mg/mL) and high (1.25 and 2.5 mg/mL) concentrations, respectively. In total, 2 phenolic acids, 12 flavonoids, along with 3 feruloyl derivatives and the plant pigment indicaxanthin, were tentatively identified by UHPLC-HRMS analysis. Phenolic acids (compounds 1 and 2) were mainly distributed in cladodes (64.6%), while flavonoids (3–14) in the flowers (81.8%). Overall, the highest amount of total flavonoids (22.76 ± 0.015 mg of quercetin equivalent [QE]/g) and total phenolics (62.80 ± 0.009 mg gallic acid equivalents [GAE]/g) was found in the flower extract. Flavonoid glycosides have not been detected in the seeds and the flesh, while the fruit seed extract contained mainly feruloyl derivatives. Our data provide convincing evidences for the lack of cytotoxic and genotoxic effects of O. ficus indica aqueous extracts and, in parallel, support the potential for further exploitation of this plant in the food supplement or functional food sector.
Oxadiazolone derivatives, new promising multi-target inhibitors against <i>M. tuberculosis</i>
Nguyen, Phuong Chi,Delorme, Vincent,Bé,narouche, Anaï,s,Guy, Alexandre,Landry, Valé,rie,Audebert, Sté,phane,Pophillat, Matthieu,Camoin, Luc,Crauste, Cé,line,Galano, Jean-Ma Elsevier 2018 Bioorganic chemistry Vol.81 No.-
<P><B>Abstract</B></P> <P>A set of 19 oxadiazolone (<B>OX</B>) derivatives have been investigated for their antimycobacterial activity against two pathogenic slow-growing mycobacteria, <I>Mycobacterium marinum</I> and <I>Mycobacterium bovis</I> BCG, and the avirulent <I>Mycobacterium tuberculosis</I> (<I>M. tb</I>) mc<SUP>2</SUP>6230. The encouraging minimal inhibitory concentrations (MIC) values obtained prompted us to test them against virulent <I>M. tb</I> H37Rv growth either in broth medium or inside macrophages. The <B>OX</B> compounds displayed a diversity of action and were found to act either on extracellular <I>M. tb</I> growth only with moderated MIC<SUB>50</SUB>, or both intracellularly on infected macrophages as well as extracellularly on bacterial growth. Of interest, all <B>OX</B> derivatives exhibited very low toxicity towards host macrophages. Among the six potential <B>OXs</B> identified, <B>HPOX</B>, a selective inhibitor of extracellular <I>M. tb</I> growth, was selected and further used in a competitive labelling/enrichment assay against the activity-based probe Desthiobiotin-FP, in order to identify its putative target(s). This approach, combined with mass spectrometry, identified 18 potential candidates, all being serine or cysteine enzymes involved in <I>M. tb</I> lipid metabolism and/or in cell wall biosynthesis. Among them, Ag85A, CaeA, TesA, KasA and MetA have been reported as essential for <I>in vitro</I> growth of <I>M. tb</I> and/or its survival and persistence inside macrophages. Overall, our findings support the assumption that <B>OX</B> derivatives may represent a novel class of multi-target inhibitors leading to the arrest of <I>M. tb</I> growth through a cumulative inhibition of a large number of Ser- and Cys-containing enzymes involved in various important physiological processes.</P> <P><B>Highlights</B></P> <P> <UL> <LI> The OX analogs represent a novel class of multi-target inhibitors against <I>M. tb.</I> </LI> <LI> Some OX inhibit either extracellular, or both intra- and extracellular <I>M. tb</I> growth. </LI> <LI> These OXs are not toxic towards host macrophages. </LI> <LI> OX probes are attractive chemical tools to identify (Ser/Cys)-enzymes in living mycobacteria. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>