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CO and CO2 Methanation Over Supported Cobalt Catalysts
Le, T. A.,Kim, M. S.,Lee, S. H.,Park, E. D. Baltzer Science Publishers 2017 Topics in catalysis Vol.60 No.9
<P>CO and CO2 methanation was investigated over cobalt catalysts supported on different supports such as gamma-Al2O3, SiO2, TiO2, CeO2, and ZrO2. Among them, the cobalt catalyst supported on the high-surface-area CeO2 was determined to be the most active for both CO and CO2 methanation. These catalytic activities increased with increasing surface area of CeO2. To increase the specific catalytic activity for CO and CO2 methanation, various Co-CeO2 catalysts with different cobalt contents were prepared with co-precipitation method. The optimum cobalt content was determined for both reactions. The prepared catalysts were characterized with N-2 physisorption, temperature-programmed reduction with H-2, pulsed-chemisorption of CO2, temperature-programmed desorption of CO2, and X-ray diffraction. The high cobalt dispersion and strong CO2 adsorption appeared to be responsible for the high catalytic activity for CO and CO2 methanation, respectively. This Co-CeO2 also showed the stable catalytic activity even after an exposure to high-temperature reaction conditions.</P>
Highly selective CO<sub>2</sub> capture by S-doped microporous carbon materials
Seema, H.,Kemp, K.C.,Le, N.H.,Park, S.W.,Chandra, V.,Lee, J.W.,Kim, K.S. Pergamon Press ; Elsevier Science Ltd 2014 Carbon Vol.66 No.-
S-doped microporous carbon materials were synthesized by the chemical activation of a reduced-graphene-oxide/poly-thiophene material. The material displayed a large CO<SUB>2</SUB> adsorption capacity of 4.5mmolg<SUP>-1</SUP> at 298K and 1atm, as well as an impressive CO<SUB>2</SUB> adsorption selectivity over N<SUB>2</SUB>, CH<SUB>4</SUB> and H<SUB>2</SUB>. The material was shown to exhibit a stable recycling adsorption capacity of 4.0mmolg<SUP>-1</SUP>. The synthesized material showed a maximum specific surface area of 1567m<SUP>2</SUP>g<SUP>-1</SUP> and an optimal CO<SUB>2</SUB> adsorption pore size of 0.6nm. The microporosity, surface area and oxidized S content of the material were found to be the determining factors for CO<SUB>2</SUB> adsorption. These properties show that the as synthesized S-doped microporous carbon material can be more effective than similarly prepared N-doped microporous carbons in CO<SUB>2</SUB> capture.
Hong, H.K.,Kang, H.S.,Le, T.C.,Choi, K.S. Academic Press 2013 Fish & shellfish immunology Vol.35 No.6
We first characterized the morphology and immune-related activities of hemocytes in the subtropical oysters Saccostrea kegaki, Ostrea circumpicta, and Hyotissa hyotis using light microscopy and flow cytometry. Hemocytes of these three oyster species were classified into three main types: 1) granulocytes containing numerous granules in the cytoplasm, 2) hyalinocytes with no or fewer granules, and 3) blast-like cells characterized by the smallest size and very thin cytoplasm. The percentage of each hemocyte population was similar in all species; hyalinocytes were the most abundant cell in the hemolymph accounting for more than 59%, followed by granulocytes (23-31%) and blast-like cells (3-5%). The size of granulocytes of S. kegaki was smaller (P < 0.05) than those of O. circumpicta and H. hyotis. Light microscopy also allowed the description of vacuolated cells characterized by large vacuoles in the cytoplasm. Flow cytometry analysis confirmed that the granulocytes of the three oyster species were the major hemocytes engaged in cellular defense with the largest lysosome content, and the most active phagocytosis activity and oxidative activity, as was previously reported in several marine bivalves. Phagocytic activity was the lowest in S. kegaki hemocytes, and PMA-stimulated oxidative activity was the lowest in H. hyotis hemocytes. Our results provide the basic information of hemocytes population of three subtropical oysters for further investigations associated with various environmental disease stresses.
Chau Nguyen N.,Anh Le T.,Vu Nguyen H.,Phuc Hoang K. 한국응용곤충학회 2022 Journal of Asia-Pacific Entomology Vol.25 No.2
Bioassays to evaluate the mortality, virulence and reproduction potentials of four indigenous EPN strains, SPQ16, S-BM12, H-KT3987 and H-CB3452 on insect larvae of mealworm (Tenebrio molitor) and greater wax moth (Galleria mellonella) revealed the highest mortality rates of two insect larvae at the highest inoculation dose of 100 IJs to range from 89 to 100 percent and 94.3–100 percent at 48 h after inoculation, respectively. Virulence was high for all nematode strains, with LC 50 values between 29.6 and 47.3 IJs/insect host. The highest IJ yields were different between nematode strains and insect host, from 66.8 × 10 3 IJs (S-PQ16) to 118.6 × 10 3 IJs (HKT3987) on T. molitor, and from 54.2 × 10 3 IJs (S-BM12) to 163.3 × 10 3 IJs (H-KT3987) on G. mellonella. The culturing cost in terms of food expenditure for rearing insect larvae varied between insect larvae and nematode strains, from 6.76 to 26.63 USD per billion IJs for nematode strains cultured on T. molitor larvae and from 3.54 to 7.81 USD per billion IJs for nematode strains cultured on G. mellonella larvae. The full cost for a nematode product of 2.5 × 10 9 IJs per hectare, produced through in vivo mass culturing, of the most efficient nematode strain, H-KT3987, was 191.3 USD, slightly cheaper than 199.4 USD for the same nematode product produced through in vitro mass culturing.
Jérôme Mounier,Geneviève Héry-Arnaud,Audrey Gouëllo,Marlène Keravec,Solène Le Gal,Grégory Pacini,Stella Debaets,Gilles Nevez,Gilles Rault,Georges Barbier 한국미생물학회 2014 The journal of microbiology Vol.52 No.4
The aim of this study was to evaluate the use of denaturinghigh-performance liquid chromatography (DHPLC) to characterizecystic fibrosis (CF) airway microbiota includingboth bacteria and fungi. DHPLC conditions were first optimizedusing a mixture of V6, V7 and V8 region 16S rRNAgene PCR amplicons from 18 bacterial species commonlyfound in CF patients. Then, the microbial diversity of 4 sputumsamples from 4 CF patients was analyzed using culturalmethods, cloning/sequencing (for bacteria only) and DHPLCpeak fraction collection/sequencing. DHPLC analysis allowedidentifying more bacterial and fungal species than the classicalculture methods, including well-recognized pathogenssuch as Pseudomonas aeruginosa. Even if a lower number ofbacterial Operational Taxonomic Units (OTUs) was identifiedby DHPLC, it allowed to find OTUs unidentified bycloning/sequencing. The combination of both techniquespermitted to correlate the majority of DHPLC peaks to definedOTUs. Finally, although Aspergillus fumigatus detectionusing DHPLC can still be improved, this techniqueclearly allowed to identify a higher number of fungal speciesversus classical culture-based methods. To conclude, DHPLCprovided meaningful additional data concerning pathogenicbacteria and fungi as well as fastidious microorganisms presentwithin the CF respiratory tract. DHPLC can be consideredas a complementary technique to culture-dependentanalyses in routine microbiological laboratories.
Le, T.K.,Jang, H.H.,Nguyen, H.T.H.,Doan, T.T.M.,Lee, G.Y.,Park, K.D.,Ahn, T.,Joung, Y.H.,Kang, H.S.,Yun, C.H. IPC Science and Technology Press ; Elsevier Scienc 2017 Enzyme and microbial technology Vol.97 No.-
<P>Enzymatic conversion of natural glycosides to their corresponding hydroxylated products using cytochromes P450 has significant advantages over synthetic chemistry and even enzyme-catalyzed glycosylation of chemicals. At present, the basic strategy for making glycosides of stilbenoid compounds is to use the glycosylation activity of enzymes, such as glycosyltransferases. Here, an efficient synthesis of a valuable (E)-astringin, a piceatannol glucoside, was developed using CYP102A1 via the highly regioselective C-3' hydroxylation of polydatin, a resveratrol glucoside. (E)-astringin is a high added value compound found in plants and wine. Benzylic hydroxylation of polydatin provides an attractive route to (E)-astringin, a catechol product. Thus far, chemical and enzymatic methods of producing (E)-astringin have not been developed. In the present study, a set of CYP102A1 mutants from Bacillus megaterium was found to catalyze regioselective hydroxylation of polydatin at the C-3' position to generate an (E)-astringin, a piceatannol glucoside. (C) 2016 Elsevier Inc. All rights reserved.</P>
Jang, H. H.,Ryu, S. H.,Le, T. K.,Doan, T. T.,Nguyen, T. H.,Park, K. D.,Yim, D. E.,Kim, D. H.,Kang, C. K.,Ahn, T. Springer Science + Business Media 2017 Biotechnology letters Vol.39 No.1
<P>A highly efficient synthesis of 5'-OH omeprazole sulfide was developed using CYP102A1 from Bacillus megaterium as a biocatalyst.</P>