Colorimetric bioassay using the catalytic ester hydrolysis by esterase-like Cu2+

( Amardeep Singh ),( Ji Hye Kim ),( Jin Kyo Jeong ),( Hae Sik Yang ) 한국환경분석학회 2013 제1회 한국분석기술단체 총 연합 합동 심포지엄 Vol.0 No.-

Seismic control of structures using sloped bottom tuned liquid dampers

Amardeep D. Bhosale,Mohan M. Murudi 국제구조공학회 2017 Structural Engineering and Mechanics, An Int'l Jou Vol.64 No.2

Earlier numerous studies have been done on implementation of Tuned Liquid Damper (TLD) for structural vibration control by many researchers. As per current review there is no significant study on a sloped bottom TLD. TLD‟s are passive devices. A TLD is a tank rigidly attached to the structure and filled partially by liquid. When fundamental linear sloshing frequency is tuned to structure‟s natural frequency large sloshing amplitude is expected. In this study set of experiments are conducted on flat bottom and sloped bottom TLD at beach slope 20º, 30º and 45º, for different types of structures, mass ratio, and depth ratio to investigate the overall effectiveness of TLD and specific effect of TLD parameters on structural response. This experimental study shows that a properly designed TLD reduces structural response. It is also observed that effectiveness of TLD increases with increase in mass ratio. In this experimental study an effectiveness of sloped bottom TLD with beach slope 30º is investigated and compared with that of flat bottom TLD in reducing the structural response. It is observed from this study that efficiency of sloped bottom TLD in reducing the response of structure is more as compared to that of flat bottom TLD. It is shown that there is good agreement between numerical simulation of flat bottom and sloped bottom TLD and its experimental results. Also an attempt has been made to investigate the effectiveness of sloped bottom TLD with beach slope 20º and 45º.

Glucose-Oxidase Label-Based Redox Cycling for an Incubation Period-Free Electrochemical Immunosensor

Singh, Amardeep,Park, Seonhwa,Yang, Haesik American Chemical Society 2013 ANALYTICAL CHEMISTRY - Vol.85 No.10

<P>Catalytic reactions of enzyme labels in enzyme-linked immunosorbent assays require a long incubation period to obtain high signal amplification. We present herein a simple immunosensing scheme in which the incubation period is minimized without a large increase in the detection limit. This scheme is based on electrochemical-enzymatic (EN) redox cycling using glucose oxidase (GOx) as an enzyme label, Ru(NH<SUB>3</SUB>)<SUB>6</SUB><SUP>3+</SUP> as a redox mediator, and glucose as an enzyme substrate. Fast electron mediation of Ru(NH<SUB>3</SUB>)<SUB>6</SUB><SUP>3+</SUP> between the electrode and the GOx label attached to the electrode allows high signal amplification. The acquisition of chronocoulometric charges at a potential in the mass transfer-controlled region excludes the influence of the kinetics of Ru(NH<SUB>3</SUB>)<SUB>6</SUB><SUP>2+</SUP> electrooxidation and also facilitates high signal-to-background ratios. The reaction between reduced GOx and Ru(NH<SUB>3</SUB>)<SUB>6</SUB><SUP>3+</SUP> is rapid even in air-saturated Tris buffer, where the faster competitive reaction between reduced GOx and dissolved oxygen also occurs. The direct electrooxidation of glucose at the electrode and the direct electron transfer between glucose and Ru(NH<SUB>3</SUB>)<SUB>6</SUB><SUP>3+</SUP> that undesirably increase background levels occur relatively slowly. The detection limit for the EN redox cycling-based detection of cancer antigen 125 (CA-125) in human serum is slightly higher than 0.1 U/mL for the incubation period of 0 min, and the detection limits for the incubation periods of 5 and 10 min are slightly lower than 0.1 U/mL, indicating that the detection limits are almost similar irrespective of the incubation period and that the immunosensor is highly sensitive.</P><P><B>Graphic Abstract</B> <IMG SRC='http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/ancham/2013/ancham.2013.85.issue-10/ac400573j/production/images/medium/ac-2013-00573j_0006.gif'></P><P><A href='http://pubs.acs.org/doi/suppl/10.1021/ac400573j'>ACS Electronic Supporting Info</A></P>

Colorimetric bioassay using the catalytic ester hydrolysis by esterase-like Cu2+

Singh, Amardeep,Patra, Srikanta,Akanda, Md. Rajibul,Yang, Haesik Elsevier 2012 Sensors and actuators. B, Chemical Vol.171 No.-

A Comparative Study on the Performance and Emission Analysis of a Dual Fuelled Diesel Engine with Karanja Biodiesel and Natural Gas

Singh, Ashish Kumar,Kumar, Naveen,Amardeep, Amardeep,Kumar, Parvesh The International Promotion Agency of Culture Tech 2016 International Journal of Advanced Culture Technolo Vol.4 No.1

In the present study, a single cylinder four stroke dual fuel diesel engine was tested to investigate the performance and emission characteristics of various test fuels. The engine was tested in dual fuel mode using diesel and Karanja biodiesel blends as pilot fuel along with Natural gas as primary fuel with a constant gas flow rate under different loading conditions. From the experimentation it was found that smoke opacity and oxides of nitrogen (NOx) are at low level for all the prepared test fuels in dual fuel mode but the emissions of carbon monoxide (CO), carbon dioxide ($CO_2$) and hydrocarbon (HC) were found higher. In comparison to diesel fuel, by increasing the blend percentage different emission parameters are found to be reduced. At different loading conditions all the test fuels show poor performance in dual fuel mode of operation when compared with single mode of operation with diesel and biodiesel. With increase in gas flow rates, except (NOx) and smoke emissions, the other emission parameters like CO, HC and $CO_2$ values increased for all test fuels. Again, all blended fuels showed lower performance compared to diesel. The maximum pilot fuel savings for diesel was found decreasing with the increase in karanja biodiesel. From the present work it may be concluded that Karanja biodiesel with Natural gas in dual mode can be can used as promising alternative for diesel with some required engine modifications and further research must be carried out to minimize the emissions of CO, HC and $CO_2$.

NK-cell trogocytosis of CD19 antigen: a rare B-ALL MRD mimicker

Devasis Panda,Amardeep Pathak,Narender Tejwani,Anurag Mehta 대한혈액학회 2022 Blood Research Vol.57 No.4

COMMON FIXED POINT FOR WEAK COMPATIBLE MAPPINGS OF TYPE ($\alpha$) IN MENGER SPACES

Sharma, Sushil,Singh, Amardeep The Youngnam Mathematical Society Korea 2007 East Asian mathematical journal Vol.23 No.1

In this paper we prove common fixed point theorem for four mappings, under the condition of compatible mappings of type ($\alpha$) in Menger space, without taking any function continuous. We improve results of Pathak, Kang and Baek [13] and Cho, Murthy and Stojakovic [37].

Electroreduction-Based Electrochemical-Enzymatic Redox Cycling for the Detection of Cancer Antigen 15-3 Using Graphene Oxide-Modified Indium–Tin Oxide Electrodes

Park, Seonhwa,Singh, Amardeep,Kim, Sinyoung,Yang, Haesik American Chemical Society 2014 ANALYTICAL CHEMISTRY - Vol.86 No.3

<P>We compare herein biosensing performance of two electroreduction-based electrochemical-enzymatic (EN) redox-cycling schemes [the redox cycling combined with simultaneous enzymatic amplification (one-enzyme scheme) and the redox cycling combined with preceding enzymatic amplification (two-enzyme scheme)]. To minimize unwanted side reactions in the two-enzyme scheme, β-galactosidase (Gal) and tyrosinase (Tyr) are selected as an enzyme label and a redox enzyme, respectively, and Tyr is selected as a redox enzyme label in the one-enzyme scheme. The signal amplification in the one-enzyme scheme consists of (i) enzymatic oxidation of catechol into <I>o</I>-benzoquinone by Tyr and (ii) electroreduction-based EN redox cycling of <I>o</I>-benzoquinone. The signal amplification in the two-enzyme scheme consists of (i) enzymatic conversion of phenyl β-<SMALL>d</SMALL>-galactopyranoside into phenol by Gal, (ii) enzymatic oxidation of phenol into catechol by Tyr, and (iii) electroreduction-based EN redox cycling of <I>o</I>-benzoquinone including further enzymatic oxidation of catechol to <I>o</I>-benzoquinone by Tyr. Graphene oxide-modified indium–tin oxide (GO/ITO) electrodes, simply prepared by immersing ITO electrodes in a GO-dispersed aqueous solution, are used to obtain better electrocatalytic activities toward <I>o</I>-benzoquinone reduction than bare ITO electrodes. The detection limits for mouse IgG, measured with GO/ITO electrodes, are lower than when measured with bare ITO electrodes. Importantly, the detection of mouse IgG using the two-enzyme scheme allows lower detection limits than that using the one-enzyme scheme, because the former gives higher signal levels at low target concentrations although the former gives lower signal levels at high concentrations. The detection limit for cancer antigen (CA) 15-3, a biomarker of breast cancer, measured using the two-enzyme scheme and GO/ITO electrodes is ca. 0.1 U/mL, indicating that the immunosensor is highly sensitive.</P><P><B>Graphic Abstract</B> <IMG SRC='http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/ancham/2014/ancham.2014.86.issue-3/ac403912d/production/images/medium/ac-2013-03912d_0005.gif'></P><P><A href='http://pubs.acs.org/doi/suppl/10.1021/ac403912d'>ACS Electronic Supporting Info</A></P>

Effect of rubber fiber size fraction on static and impact behavior of self-compacting concrete

Thakare, Akshay A.,Siddique, Salman,Singh, Amardeep,Gupta, Trilok,Chaudhary, Sandeep Techno-Press 2022 Advances in concrete construction Vol.13 No.6

The conventional disposal methods of waste tires are harmful to the environment. Moreover, the recycling/reuse of waste tires in domestic and industrial applications is limited due to parent product's quality control and environmental concerns. Additionally, the recycling industry often prefers powdered rubber particles (<0.60 mm). However, the processing of waste tires yields both powdered and coarser (>0.60 mm) size fractions. Reprocessing of coarser rubber requires higher energy increasing the product cost. Therefore, the waste tire rubber (WTR) less favored by the recycling industry is encouraged for use in construction products as one of the environment-friendly disposal methods. In this study, WTR fiber >0.60 mm size fraction is collected from the industry and sorted into 0.60-1.18, 1.18-2.36-, and 2.36-4.75-mm sizes. The effects of different fiber size fractions are studied by incorporating it as fine aggregates at 10%, 20%, and 30% in the self-compacting rubberized concrete (SCRC). The experimental investigations are carried out by performing fresh and hardened state tests. As the fresh state tests, the slump-flow, T500, V-funnel, and L-box are performed. As the hardened state tests, the scanning electron microscope, compressive strength, flexural strength and split tensile strength tests are conducted. Also, the water absorption, porosity, and ultrasonic pulse velocity tests are performed to measure durability. Furthermore, SCRC's energy absorption capacity is evaluated using the falling weight impact test. The statistical significance of content and size fraction of WTR fiber on SCRC is evaluated using the analysis of variance (ANOVA). As the general conclusion, implementation of various size fraction WTR fiber as fine aggregate showed potential for producing concrete for construction applications. Thus, use of WTR fiber in concrete is suggested for safe, and feasible waste tire disposal.

Low-Interference Washing-Free Electrochemical Immunosensor Using Glycerol-3-phosphate Dehydrogenase as an Enzyme Label

Dutta, Gorachand,Park, Seonhwa,Singh, Amardeep,Seo, Jeongwook,Kim, Sinyoung,Yang, Haesik American Chemical Society 2015 ANALYTICAL CHEMISTRY - Vol.87 No.7

<P>In washing-free electrochemical detection, various redox and reactive species cause significant interference. To minimize this interference, we report a washing-free electrochemical immunosensor using flavin adenine dinucleotide (FAD)-dependent glycerol-3-phosphate dehydrogenase (GPDH) and glycerol-3-phosphate (GP) as an enzyme label and its substrate, respectively, because the reaction of FAD-dependent dehydrogenases with dissolved O<SUB>2</SUB> is slow and the level of GP preexisting in blood is low (<0.1 mM). A combination of a low electrocatalytic indium–tin oxide (ITO) electrode and fast electron-mediating Ru(NH<SUB>3</SUB>)<SUB>6</SUB><SUP>3+</SUP> is employed to obtain a high signal-to-background ratio via proximity-dependent electron mediation of Ru(NH<SUB>3</SUB>)<SUB>6</SUB><SUP>3+</SUP> between the ITO electrode and the GPDH label. Electrochemical oxidation of GPDH-generated Ru(NH<SUB>3</SUB>)<SUB>6</SUB><SUP>2+</SUP> is performed at 0.05 V vs Ag/AgCl, at which point the electrochemical interference is very low. When a washing-free immunosensor is applied to cardiac troponin I detection in human serum, the calculated detection limit is approximately 10 pg/mL, indicating that the immunosensor is very sensitive in spite of the use of washing-free detection with a short detection period (10 min for incubation and 100 s for electrochemical measurement). The low-interference washing-free electrochemical immunosensor shows good promise for fast and simple point-of-care testing.</P><P><B>Graphic Abstract</B> <IMG SRC='http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/ancham/2015/ancham.2015.87.issue-7/ac504485a/production/images/medium/ac-2014-04485a_0007.gif'></P><P><A href='http://pubs.acs.org/doi/suppl/10.1021/ac504485a'>ACS Electronic Supporting Info</A></P>