Alteration of Apoptosis during Differentiation in Human Dental Pulp-Derived Mesenchymal Stem Cell

Hyeon-Jeong Lee,Byung-Joon Park,Ryoung-Hoon Jeon,Si-Jung Jang,Young-Bum Son,Sung-Lim Lee,Gyu-Jin Rho,Seung-Joon Kim,Won-Jae Lee 한국동물생명공학회(구 한국동물번식학회) 2019 Journal of Animal Reproduction and Biotechnology Vol.34 No.1

Because mesenchymal stem cells (MSCs) maintain distinct capacities with respect to self-renewal, differentiation ability and immunomodulatory function, they have been highly considered as the therapeutic agents for cell-based clinical application. Of particular, differentiation condition alters characteristics of MSCs, including cellular morphology, expression of gene/protein and cell surface molecule, immunological property and apoptosis. However, the previous results for differentiation-related apoptosis in MSCs have still remained controversial due to varied outcomes. Therefore, the present study aimed to disclose periodical alterations of pro- and anti-apoptosis in MSCs under differentiation inductions. The human dental pulp-derived MSCs (DP-MSCs) were differentiated into adipocytes and osteoblasts during early (1 week), middle (2 weeks) and late (3 weeks) stages, and were investigated on their apoptosis-related changes by Annexin V assay, qRT-PCR and western blotting. The ratio of apoptotic cell population was significantly (p < 0.05) elevated during the early to middle stages of differentiations but recovered up to the similar level of undifferentiated state at the late stage of differentiation. In the expression of mRNA and protein, whereas expressions of pro-apoptosis-related makers (BAX and BAK) were not altered in any kind and duration of differentiation inductions, anti-apoptosis marker (BCL2) was significantly (p < 0.05) elevated even at the early stage of differentiations. The recovery of apoptotic cell population at the late stage of differentiation is expected to be associated with the response by elevation of anti-apoptotic molecules. The present study may contribute on understanding for cellular mechanism in differentiation of MSCs and provide background data in clinical application of MSCs in the animal biotechnology to develop effective and safe therapeutic strategy.

A Study on the Usefulness of Ischemia-modified Albumin (IMA) in the Setting of a Symptom-Limited Exercise Stress Test to Detect Myocardial Ischemia

( Joon Sang Lee ),( Jun Hyok Oh ),( Byung Jae Ahn ),( Seong Ho Kim ),( Moo Young Kim ),( Woo Hyung Bae ),( Hyeon Gook Lee ),( Jun Kim ),( June Hong Kim ),( Kook Jin Chun ),( Taek Jong Hong ),( Yung Wo 대한내과학회 2005 대한내과학회 추계학술대회 Vol.69 No.10

Successful treatment of granuloma annulare with UVA-1 photothrapy

( Sang Hwa Han ),( Kang Hoon Lee ),( Joon Hee Kim ),( Myeong Hyeon Yang ),( Min Soo Jang ),( Kee Suck Suh ) 대한피부과학회 2015 대한피부과학회 학술발표대회집 Vol.67 No.1

Granuloma annulare (GA) is a benign, granulomatous skin disease that presents clinically as papules and plaques, often in an annular configuration. The histopathologic findings of GA show central core of altered collagen surrounded by a wall of palisaded histiocytes or interstitial, nonpalisaded pattern of inflammation with histiocytes infiltrating among fragmented collagen bundles. Ultraviolet (UV) radiation has been used for decades to treat variable skin diseases. The therapeutic effect of UVA-1 is related to T-cell apoptosis, increased collagenase activity, and it penetrates the dermis more deeply than UVB and PUVA. A 73-year-old female patient presented with multiple erythematous annular plaques and patches on the trunk. Histopathologic findings showed intact epidermis, palisading infiltrate of inflammatory cells with degeneration of collagen, and swollen and intensely eosinophilic collagen fibers with dense interstitial histiocytic infiltrate. On the basis of these clinical and histological findings, the diagnosis of granuloma annulare was made. The patient was treated with medium-dose (65 J/cm2) UVA-1 phototherapy 3-4 times a week. A significant improvement in skin lesions was achieved after 17th UVA-1 phototherapy. Herein, we report a case of granuloma annulare successfully treated with UVA-1 phototherapy.

Successful treatment of cutaneous polyarteritis nodosa with pentoxifylline

( Sang Hwa Han ),( Kang Hoon Lee ),( Joon Hee Kim ),( Myeong Hyeon Yang ),( Min Soo Jang ),( Kee Suck Suh ) 대한피부과학회 2015 대한피부과학회 학술발표대회집 Vol.67 No.1

Cutaneous polyarteritis nodosa (PAN) is a vasculitis of small-to-medium-sized arteries with chronic benign course without systemic involvement. Clinically, tender subcutaneous nodule, livedo reticularis, and cutaneous ulcers can be seen. Histopathologically, degeneration of the arterial wall with deposition of fibrinoid material, complete occlusion of the lumen with thrombus, and fibroblastic proliferation into the perivascular area can be seen. Systemic steroid and non-steroidal anti-inflammatory drugs have formed the mainstay of treatment of cutaneous PAN, and pentoxifylline also can be used. Pentoxifylline is a synthetic xanthine derivative with hemorheological properties. A 34-year-old male patient presented with multiple brownish subcutaneous nodules on the lower legs. Histopathologic findings showed deep perivascular inflammation, degeneration of the arterial wall with deposition of fibrinoid material, and infiltrate of neutrophils, lymphocytes, and histiocytes within and around the arterial wall. On the basis of these clinical and histological findings, the diagnosis of cutaneous PAN was made. The patient was treated with pentoxifylline, resulting in a significant improvement in skin lesions. The improvement of blood flow and the anti-inflammatory effects of pentoxifylline may have played role in the resolution of the disease in this patient. Herein, we report a case of cutaneous PAN successfully treated with pentoxifylline.

Clinical and dermoscopic features of Clark nevus in Korean

( Joon Hee Kim ),( Myeong Hyeon Yang ),( Kang Hoon Lee ),( Sang Hwa Han ),( Min Soo Jang ),( Kee Suck Suh ) 대한피부과학회 2015 대한피부과학회 학술발표대회집 Vol.67 No.2

Background: Clark nevus, also known as dysplastic and atypical nevus, may be confused with melanoma. Dermoscopy has proven to be a useful, noninvasive tool in the differential diagnosis of pigmented lesions. However, to our knowledge, few studies about clinical and dermoscopic features of Clark nevus have been reported in Korea. Objectives: To analyse clinical and dermoscopic features of Clark nevus. Methods: We studied clinically 9 atypical lesions (more than 6mm in diameter, color variegations, irregularity of the border and not congenital nevus) that were histologically diagnosed as dysplastic nevus. The lesions were classified by clinical and dermoscopic features, and evaluated with ABCD (asymmetry, border, color, and differential structures) rule to calculate total dermoscopic score (TDS). Results: Clinical type of 6 lesions was lentiginous type and 3 lesions was fried-egg type. Using dermoscopic classification, lesions were classified as follows: 3 (33.3%) as reticular-globular, 2 (22.2%) as reticular-homogenous, 2 (18.2%) as reticular, 1 (9.1%) as globular-homogenous, and 1 (9.1%) as globular. The mean TDS was 5.0 (range: 4.3 to 6.5). TDS of 8 lesions was ranged from 4.3 to 5.5 and 1 lesion was higher than 6.0. Conclusion: The predominant clinical and dermoscopic type of Clark nevus is lentiginous type and reticular- globular type respectively, and TDS is a useful diagnostic method for exclusion of melanoma.

P026 : Pivotal role of potassium iodide in mitogenic effect on proliferation and migration in vitro and epidermal and dermal wound healing in vivo

( Joon Hong Min ),( Jung In Kim ),( Jung Min ),( Hyun Min Seo ),( Sang Hyeon Hwang ),( Heun Joo Lee ),( Yoon Hwan Kim ),( Joon Lee ),( Seung Jae Lee ),( Ji Hye Park ),( Ga Young Lee ),( Soo Hong Park 대한피부과학회 2013 대한피부과학회 학술발표대회집 Vol.65 No.2

Background: Potassium iodide(KI) has been used for various skin problems such as erythema nodosum, chronic vasculitis and deep fungal infections. However, the mechanisms of KI action for those diseases were unclear. Objectives: The aim of this study is to investigate the proliferative and migratory effects on KI in keratinocyte progenitor cells (KSC) and human dermal fibroblasts (HDF), and reveal the cellular alteration which involved in the KI activation and wound healing effect of the KI. Methods: We studied the effect of KI (0.01, 0.1, and 1mM) on cell proliferation, migration and hydrogen peroxideinduced apoptosis using a KSC and HDF cell lines. Animal murine model was used to investigate the effect of KI on excision wound. Results: KI showed stimulation effects on the cell proliferation and migration in HDF. KI reduced apoptotic cell death and increased cell survival in hydrogen peroxide treated HDF and KSC. Furthermore, the topical application of KI accelerates the healing of epidermal and dermal wounds in murine model. Conclusion: In our results, KI showed cell stimulation and protection effects, which induced fast wound recovery. We suggest these effects would be considered for one of mechanism of pharmacological action of KI.

P223 : Clinical and histologic study of pigmented purpuric dermatoses

( Sang Hyeon Hwang ),( Jung In Kim ),( Jung Min ),( Hyun Min Seo ),( Heun Joo Lee ),( Yoon Hwan Kim ),( Joon Hong Min ),( Joon Lee ),( Seung Jae Lee ),( Ji Hye Park ),( Ga Young Lee ),( Soo Hong Park 대한피부과학회 2013 대한피부과학회 학술발표대회집 Vol.65 No.2

Background: Pigmented purpuric dermatoses (PPD) are chronic and relapsing disorders characterized by asymmetrical rash of petechial and pigmentary macules confined to the lower limbs. Clinical and histopathological study of PPD has not been conducted in Korea. Objectives: The aim of this study was to investigate the clinical and histopathological features of PPD in Koreans. Methods: We reviewed all the medical records, photographs and histopathological slides of 35 patients who had been diagnosed with PPD at Kangbuk Samsung Hospital between January 2008 and June 2013. Results: The mean duration of disease was 26.5 months, and both legs were most frequently involved site (85.7%). Most of the patients were asymptomatic (74.2%). Patients were treated with topical or systemic steroid in 30 cases (85.7%) and 9 patients were added tranexamic acid but there was no difference in the clinical course according to the treatment. The identified etiologic factors were orthostatic pressure (17.1%) and exercise (5.7%). Patients without etiologic factors had more frequent incidence of medical problems (p<0.05). The major microscopic features of the lesions of PPD were perivascular lymphohistiocytic infiltration, extravasated erythrocytes and hemosiderin deposition. Conclusion: The results of this study may help clinicians to diagnose PPD and it should be included in differential diagnosis when cutaneous lesions especially show asymptomatic persisting rash on both lower legs.

The association between benign symmetric lipomatosis and heavy alcohol intake

( Joon Hee Kim ),( Myeong Hyeon Yang ),( Kang Hoon Lee ),( Sang Hwa Han ),( Min Soo Jang ),( Kee Suck Suh ) 대한피부과학회 2015 대한피부과학회 학술발표대회집 Vol.67 No.2

Background: Benign symmetric lipomatosis (BSL) is rare disorder characterized by painless, enlarging, diffuse, and symmetric deposits of subcutaneous fat predominantly around the neck, shoulders, and upper extremities. Objectives: The purpose of this study is to analyse the clinical and laboratory features of BSL and its association with alcoholism. Methods: Sixteen patients, histologically diagnosed as BSL, were included in this study. We assessed alcohol intake to evaluate whether patient is high risk drinker ( ? 7 drinks of alcohol for male and ? 5 drinks of alcohol for female on a single day at least 2 times a week) and laboratory findings of them. Results: The patients were between 28 and 63 years of age with mean age of 46 years. Thirteen of the 16 patients were male, and 3 were female. The common sites of occurrence were the arm, leg, and abdomen. Five of 16 patients were presented with tender nodules. Seven patients (43.8%) were high risk drinker and 5 patients (31.3%) were non-drinker. Percentage of high risk drinker among BSL included in this study was higher than that among Korean adult (11.9%). In laboratory findings, abnormal values in liver function tests were observed in 7 patients (43.8%) and hyperlipidemia was observed in 6 patients (37.5%). Conclusion: Benign symmetric lipomatosis is frequently associated with heavy alcohol intake, abnormal liver function and hyperlipidemia.

Validation of the Simplified Palliative Prognostic Index to Predict Survival for Advanced Cancer Patients in Home Hospice Setting

Hyeon-Jeong Yang,Seok Joon Yoon,Jong Sung Kim,Sung Soo Kim,Jin Gyu Jung,Won Yoon Suh,Sa-Mi Lee,Hyun Gu Kim,YongwooLee 대한가정의학회 2021 Korean Journal of Family Medicine Vol.42 No.4

Background: The simplified Palliative Prognostic Index (sPPI) substitutes a single item from the Communication Capacity Scale (CCS) for the delirium item of the original PPI. This study aimed to examine the validity of the sPPI for patients with advanced cancer in a home-based hospice care setting. Methods: This study included 75 patients with advanced cancer who received home-based hospice care. We used medical records maintained by professional hospice nurses who had visited the patients in their homes. Based on their sPPI score, patients were divided into three groups—A (<4), B (≥4 and <6), and C (≥6)—to compare survival. Further, we investigated the sPPI’s accuracy using the area under the receiver operating characteristic curve (AUC) and sensitivity and specificity for 3- and 6-week survival. We used three sPPIs including different substitutions for the delirium item (two methods using the CCS and one using the Korean Nursing Delirium Screening Scale). Results: The median survival was 60–61 days for group A, 27–30 days for group B, and 12–16 days for group C. The difference in survival was significant (P<0.05). The AUC was 0.814–0.867 for 3-week survival and 0.736–0.779 for 6-week survival. For 3- and 6-week survival, prognostic prediction showed sensitivities of 76.2%–90.9% and 76.3%– 86.8%, and specificities of 64.2%–88.7% and 51.4%–70.3%, respectively. Conclusion: The sPPI, which is measured by professional hospice nurses, has acceptable validity to predict survival for patients with advanced cancer in a home hospice setting in South Korea.

Effects of N-acetyl Cysteine as Precursor of GSH in RA-induced Neuronal Differentiation of P19 ESCs

Joon Yup Kim,Jiae Park,Yoo Hun Noh,Do Hee Kim,Ok Hyeon Kim,Yoon Hee Chung,Kyung Yong Kim,Seung Ho Han,Sung Su Kim,Won Bok Lee 중앙대학교 의과대학 의과학연구소 2013 中央醫大誌 Vol.38 No.2

N-acetyl cysteine (NAC), estradiol and melatonin are well-known as antioxidant, and these reagents have a strong influence on many cellular events. Therefore, we compared effects of NAC in Retinoic acid (RA)-induced embryonic body's (EB) formation and neuronal differentiation of P19 embryonic stem cells (P19 ESCs) with estradiol and melatonin. NAC dramatically increased EB formation and neuronal differentiation in terms of neuronal marker, MAP-2, and neuronal maturation. However, in additional treated groups with estradiol and melatonin, no differences were founded as contrasted with NAC treatment. Furthermore, in NAC-enhanced neuronal differentiation, intracellular glutathione (GSH) contents was only increased, and co-treatment with buthionine sulphoximine (BSO), a GSH-synthesis inhibitor, effectively reduced the EB formation and neuronal differentiation. These results demonstrated that NAC increase EB formation and neuronal differentiation by up-regulation of intracellular GSH contents. NAC-enhanced neuronal differentiation effects may be as donor of GSH, not as antioxidant.