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뷰틸레이트하이드록시애니솔이 서방화된 PLGA 필름을 이용한 골수간엽줄기세포의 신경세포화
장우영 ( Woo Young Jang ),김선화 ( Seon Hwa Kim ),이일우 ( Il Woo Lee ),김문석 ( Moon Suk Kim ),이종문 ( John M. Rhee ),강길선 ( Gil Son Khang ),이해방 ( Hai Bang Lee ) 한국조직공학과 재생의학회 2005 조직공학과 재생의학 Vol.2 No.2
It has been widely recognized that bone marrow stromal cells (BMSCs) can be differentiated into osteoblasts, chondrocytes, and neuronal cells under the controlling of culture condition. In the present study, poly(L-lactide- co-glycolide) (PLGA) film was fabricated to release butylated hydroxyanisole (BHA) for the purpose of neurogenesis of BMSCs. PLGA films loaded BHA were manufactured as the concentrations of 200, 400, and 600 ? M/0.2 g PLGA and characterized by scanning electron microscope (SEM). The inverted microscope was used for the observation of the morphology of BMSCs cultivated on BHA/PLGA film during 5 days. MTT assay used for the measurement of the viability of the cells and cytotoxicity. After 5 days, differentiation of neuronal cells were confirmed by means of staining such as neurofilament (NF) and neuron-specific enolase (NSE). The changes of BMSCs to neuronal cell observed onto the fabricated PLGA film from after 1 day by the stimulation of BHA. The more the concentration of BHA increased, the less the viability of the cells, however, the cytotoxicity of the cells showed little effect until up to 600 ?M/0.2 g PLGA of concentration from the result of immunohistochemistry. It can be concluded that the controlled release of BHA from BHA loaded PLGA film might be differentiated BMSCs to neuronal cells due to the diffusion of BHA and biodegradation of PLGA. This study suggested that BHA/PLGA film is released the desired period in delivery system and useful neuronal growth culture of BMSCs for the application of neuronal regenerative medicine.
함량별 DBP 스폰지에서 골수간엽줄기세포를 이용한 조직공학적 골분화 유도
홍희경 ( Hee Kyung Hong ),김순희 ( Soon Hee Kim ),이선경 ( Seon Kyoung Lee ),김대성 ( Dae Sung Kim ),최진희 ( Jin Hee Choi ),이동원 ( Dong Won Lee ),이종문 ( John M Rhee ),손영숙 ( Young Sook Son ),강길선 ( Gil Son Khang ) 한국조직공학·재생의학회 2009 조직공학과 재생의학 Vol.6 No.4
Demineralized bone particle(DBP) that affects to cell proliferation and differentiation has been used as biomaterials. Bone marrow stromal cells(BMSCs) exhibit multiple traits of a stem cell population, and they can expand many times in vitro and be induced to differentiate into multiple cell types. In this study, we evaluated the effect of type of medium and contents DBP sponge on bone differentiation of rat BMSCs. Growth medium was consisted of DMEM, 10% FBS, 1% antibiotic antinycotics and Osteogenesis medium added to β-glycerophosphate 10 mM, L-ascorbic acid, dexamethasone 10-8 M, vitamin D3 10-8 M. DBP sponge were prepared by freeze-drying method from 1, 2 and 3 wt% DBP solution. BMSCs were harvested from the femurs of adult female Fischer rat. The effect of DBP sponge on the proliferation and ostegenesis of rat BMSCs were assessed in culture using the MTT assay, SEM, ALP assay, and RT-PCR was conducted to confirm mRNA expression of ALP and osteocalcin for osteogenic marker. According to our results, the cell viability and the quantity of ALP in 3 wt% DBP sponge were superior to other sponges. The osteogenesis medium provided the better osteogenesis effect than the growth medium. We concluded that osteogenesis medium and 3 wt% DBP sponge will be useful to bone differentiation using 3 wt% DBP sponge.
SBR 컴파운드의 가황 및 기계적 성질에 미치는 카본블랙 표면의 화학적 개질의 영향
나창운(Chang Woon Nah),이종문(John M . Rhee),김완두(Wan Doo Kim),강신영(Shin Young Kaang),장영욱(Young Wook Chang),박수진(Soo Jin Park) 한국고무학회 2001 엘라스토머 및 콤포지트 Vol.36 No.1
To see the effect of chemical surface modification, the carbon black surfaces were treated with three types of chemicals (KOH, H₃PO₄, and benzene). Vulcanization and mechanical properties of a styrene-butadiene rubber (SBR) were investigated depending on the chemical treatments. The surface free energy increased considerably with the treatments by both the acid (HCB) and base (KCB), but only a slight increase was observed for benzene treatment(BCB). The BCB showed the highest level of the London dispersive component. The vulcanization reaction was found to be faster in the order of KCB-SBR$gt;BCB-SBR$gt;VCB-SBR(virgin)$gt;HCB-SBR. The difference in minimum and maximum torque of rheocurve, representing the degree of crosslinking, was found to be higher for the BCB-SBR compared to those of VCB-SBR, KCB-SBR, and HCB-SBR. The BCB-SBR and KCB-SBR showed the improved tensile and dynamic mechanical properties. A linear relationship was found to exist between the London dispersive component of surface free energy and mechanical properties.
EPDM/PP계 열가소성 가교체의 유변특성 및 물성에 미치는 조성의 영향
최대환 ( Dae Whan Choi ),임성담 ( Sung Dam Lim ),이종문 ( John M. Rhee ),김완두 ( Wan Doo Kim ),나창운 ( Chang Woon Nah ) 한국공업화학회 2003 공업화학 Vol.14 No.4
ethylene-propylene diene ter-monomer (EPDM)/polypropylene (PP)계 thermoplastic olefin (TPO)와 thermoplastic vulcanizate (TPV)의 유변특성 및 기계적 물성에 미치는 EPDM/PP 조성비, 오일함량, 카본블랙 함량의 영향을 조사하였다. TPO 및 TPV는 고분자 수지의 전형적인 power law 형태의 pseudoplastic 흐름거동을 따르는 것을 확인하였다. EPDM의 함량이 높을수록 높은 점도를 나타내었다. 오일함량 증가에 따라 선형적인 점도하락을 보인반면, 카본블랙은 그다지 큰 영향을 보이지 않았다. TPO의 경우 EPDM 합량 증가에 따라 인장강도의 선형적인 감소를 나타낸 반면, TPV의 경우 EPDM 함량이 65 wt%까지는 거의 유사한 결과를 보인 후 그 이상에서는 급격한 감소를 나타내었다. TPO 및 TPV의 모듈러스는 Coran-Patel 모델에 잘 따르는 것으로 확인되었다. TPO의 경우 EPDM의 함량이 70 wt% 이상에서 상변환이 일어나지만, TPV의 경우는 동일조성에서 상변환이 관찰되지 않았다. Effects of ethylene-propylene diene ter-monomer (EPDM)/polypropylene (PP) composition, oil and carbon black contents on the rheological and mechanical properties of thermoplastic olefin (TPO) and thermoplastic vulcanizate (TPV) based on EPDHPP were investigated. It was found that the flow behavior of TPO and TPV followed the power-law behavior showing a typical pseudoplastic characteristics. Higher shear viscosity was observed with increased EPDM content. The shear viscosity decreased with increasing oil content, but no considerable effect was found by the addition of carbon black. Tensile strength of TPO decreased linearly with increasing EPDM content, while that of TPV maintained until 65 wt% and then rapidly decreased with further increase of EPDM content. Tensile moduli of TPO and TPV could be well predicted by the Coran-Patel model. When the content of EPDM was increased to about 70wt%, phase inversion was observed for TPO, but it was not found for TPV.