Nuclear Factor kappa B 억제를 통한 인진추출물의 inducible Nitric Oxide synthase 및 Cytokine 억제효과

김대성,박숙자,조미정,박상미,김상찬,변성희,Kim, Dae-Sung,Park, Sook-Jahr,Jo, Mi-Jeong,Park, Sang-Mi,Kim, Sang-Chan,Byun, Sung-Hui 대한한의학방제학회 2009 大韓韓醫學方劑學會誌 Vol.17 No.2

Herba Artemisiae Capillaris is the dried bud of Artemisia capillaris Thunb, which has been used for expelling heat to loosen the bowels and normalizing gallbladder function to cure jaundice in traditional oriental medicines. In the present study, we evaluated the anti-inflammatory effects of the aqueous extracts of Herba Artemisiae Capillaris (HAC) in LPS-activated Raw 264.7 cells. Cells were treated with $1\;{\mu}g/ml$ of LPS 1 h before adding HAC extract. Cell viability was determined by MTT assay, and the relative level of NO was measured with Griess reagent. TNF-$\alpha$, IL-$1{\beta}$, and IL-6 cytokines were detected by ELISA. During the entire experimental period, all three doses of HAC extract (0.03, 0.10 and 0.30 mg/ml) had no significant cytotoxicity. LPS-activated cells showed increased NO levels and iNOS expressions compared to control. However, these increases were dramatically attenuated by treatment with HAC extract. Moreover, the inhibitory effects of HAC extract occurred in a dose-dependent manner. In addition, HAC extract reduced the translocation of $NF{\kappa}B$ into nuclear. HAC reduced production of IL-$1{\beta}$ and IL-6 by LPS, although it had no effects on TNF-$\alpha$. These results demonstrate that liquiritigenin exerts anti-inflammatory effects, which results from the inhibition of $NF{\kappa}B$ activation in macrophages, thereby decreasing production of iNOS and proinflammatory cytokines. Taken together, these results indicate that the aqueous extracts of Herba Artemisiae Capillaris warrant further development as an anti-inflammatory agent for the treatment of gram-negative bacterial infections.

A Study on the Seropositivity of HBsAg among Biennial Health Examinees ; A Nation-wide Multicenter Survey

김대성,김영식,김재용,안윤옥,Kim, Dae-Sung,Kim, Young-Sik,Kim, Jae-Yong,Ahn, Yoon-Ok The Korean Society for Preventive Medicine 2002 예방의학회지 Vol.35 No.2

목적 : 1998년 한국인 성인에서의 혈청 HBsAg의 양성률을 추정하는 것을 일차적 목적으로 하며, HBsAg 양성률의 연령별로 분포, 지역적 차이, 과거 간질환력, 만성간질환 가족력 및 예방접종과의 관련성을 파악하며 또한 양성자를 6개월간 추적후 재검사 하여 B형 간염의 만성보균율을 파악하고자 하는 것을 이차적 목적으로 하였다. 방법 : 1998년 전국 10개 지역에서 의료보험관리공단의 정기건강검진 수진자를 대상으로 하여 HBsAg의 혈청유병률을 조사하였다. HBsAg 양성인 사람들을 대상으로 6개월 이상 추적조사하여 B형간염 만성보균율을 파악하였다. 총 1,816명에 대한 혈청과 설문서가 수집되었다. HBsAg는 RIA로 측정하였다. 결과 : HBsAg의 혈청유병률은 5.5%(95% CI-4.5-6.6)였으며 남자에서 7.4%(95% CI=5.8-9.4), 여자에서 3.6%(95% CI=2.5-5.0)로 나타났다. 급성간질환 과거력과 만성간질환 가족력을 HBsAg 혈청검사결과와 비교한 결과 유의한 관련성이 있는 것으로 나타났다. HBsAg 양성자에서 6개월 후에 음성으로 전환한 사람은 3.2%(95% CI=0.1-16.7)였으며 따라서 B형간염의 만성보균율은 5.3%(95% CI=3.7-6.6)로 추정되었다. 결론 : 본 연구결과에서는 HBsAg의 양성률이 1980년대의 연구결과들에 비하여 비교적 낮게 추정되었으며 이는 특히 여성과 젊은 연령층에서 두드러지게 나타났다. 그러나 우리나라에서의 간암 및 만성간질환의 공중보건학적 중요성을 고려하면 지속적인 간염발생의 예방대책이 필요하다고 할 수 있다. Objective : The primary objective of this study was to estimate the prevalence of HBsAg-positives in the late 1990's among Korean adults. In addition, we evaluated the association of age, a residential area, a vaccination rate, a family history of chronic liver diseases and a past history of acute liver disease with the seropositivity of HBsAg, and estimated the prevalence of chronic HBV infection by follow-up for 6 month or more. Methods : A total of 10 areas, six metropolitan and four small cities, were selected. In each cities, one health screening center was selected for recruitment of study subjects. The study subjects were enrolled from a general health examination program that is provided by medical insurance companies. Questionnaires on various risk factors were administered to the study subjects. Sera was drawn and tested for HBsAg by radioimmunoassay. HBeAg and ALT were also tested for those of HBsAg positive. The HBsAg positives was retest for HBsAg 6 months later Results : Among the study subjects (n= 1816), the seroprevalence of HBsAg was 5.5% (95% CI=4.5%-6.6%), 7.4% in men (95% CI=5.8-9.4) and 3.6% in women (95% CI=2.5-5.0). A past history of acute liver disease and a family history of chronic liver diseases was shown to be risk factors for HBsAg positivity. Among the 31 HBsAg-positives, negative seroconversion rate was estimated to be 3.2%, Thus, prevalence of chronic HBV infection was estimated to be 5.3% (95% CI=3.7-6.6). Conclusion : In this study, the HBsAg seroprevalence rate was lower than that of the other studies in 1980's, particularly in young adult and women. Considering the public health importance of liver cancer and chronic liver diseases, the further effort is needed to prevent and reduce the HBV infection.

MSH에 의해 자극된 B16F10세포에서 사간(射干)의 멜라닌 합성 억제 효과

김대성,성병곤,이장천,이부균,우원홍,임규상,Kim, Dae-Sung,Sung, Byung-Gon,Lee, Jang-Cheon,Lee, Boo-Kyun,Woo, Won-Hong,Lim, Kyu-Sang 대한한방안이비인후피부과학회 2011 한방안이비인후피부과학회지 Vol.24 No.1

Objective : The present study was designed to assess the potential inhibitory activity of an ethanol extract of Belamcandae Rhizoma (EBR) on the alpha-melanocyte stimulating hormone (${\alpha}$-MSH)-induced melanogenesis signal pathway in B16F10 melanoma cells. Methods : Several experiments were performed in B16F10 melanoma cells. We studied tyrosinase activity, melanin content, cell-free tyrosinase activity and DOPA stain, and performed Western blots and RT-PCR for proteins and mRNA involved in melanogenesis. Results : ${\alpha}$-MSH-induced tyrosinase activity and melanin content were inhibited significantly by EBR. EBR markedly suppressed the protein expression level of tyrosinase in B16F10 melanoma cells. On the other hand, the expression of tyrosinase-related protein-1 (TRP-1) and -2 (TRP-2; DCT) were not affected by EBR. To elucidate the mechanism of the depigmenting property of EBR, we examined the involvement EBR in cAMP response element binding (CREB) protein phosphorylation and microphthalmia-associated transcription factor (MITF) signalling induced by ${\alpha}$-MSH. EBR did not regulate CREB phosphorylation and MITF expression by ${\alpha}$-MSH. Nevertheless, the mRNA expression of tyrosinase was significantly attenuated by EBR treatment without changes in the expression of TRP-1 and -2 mRNA. Conclusion : Our study suggested that EBR inhibits ${\alpha}$-MSH-induced melanogenesis by suppressing tyrosinase mRNA.

소프트웨어 품질이 소비자 구매 행동에 미치는 영향

김대성,오성배,Kim, Dae-Sung,Oh, Sung-Bae 한국벤처창업학회 2009 벤처창업연구 Vol.4 No.1

This study is The Effect of the on the purchase behavior of the Software Quality. To have purchased the software in a Consumer surveys were conducted. The following is a summary of results of this research. First, the software quality and significant consumer purchasing behavior (+) of influence. Functionality, usability, portability of software quality attributes the impulse buying and buy more of both the (+) showed the influence. Second, software quality is urge consumers to purchase significant (+) of influence. Functionality, reliability, usability, portability of software quality (+) showed the influence. Third, the software quality In the consumer buy more significant Chung (+) showed the influence. Software quality, functionality, usability, portability name (+) influence. In conclusion, the quality of the software in consumer purchasing behavior (+) were of significant influence. In particular, factors that functionality, usability factors, portability factor in the relatively large influence on the purchasing behavior. Software technology development, planning, development, testing, and in each step must keep in mind the following. First, the software, when used in certain conditions, Inherent ability to meet the needs and requirements set forth to provide the appropriate functionality and accuracy as the ability of software products each other with the capabilities to the effective and thorough security features should be required. Second, software, a user-specified criteria to use in understanding and learning, and must, and the ability to prefer. Software for users to understand and easy to use, whether the check. In particular, understanding the interface, Help understanding, understand the input and output data, and interface consistency, the user information, the message must be equipped and easy to understand. In addition, ease of learning to be self-learning, and help ease of access shall be required. Third, in certain circumstances, a different environment to transfer be equipped with the functionality of the software. Applying data structures and application environment, helping transplant convenience must keep in mind sex.

만성간질환 위험요인에 대한 코호트연구: 잠재적 발병자 집단을 감안한 분석전략

김대성,김동현,배종면,신명희,안윤옥,이무송,Kim, Dae-Sung,Kim, Dong-Hyun,Bae, Jong-Myun,Shin, Myung-Hee,Ahn, Yoon-Ok,Lee, Moo-Song 대한예방의학회 1999 예방의학회지 Vol.32 No.4

연구자들은 만성간질환의 코호트연구에서 잠재적 발병자 집단이 포함되어 있을 때 분석 결과의 비뚤림을 평가하고 적절한 분석전략을 제안하기 위하여 본 연구를 수행하였다. 1993년 현재 서울코호트로 구축된 14,529명을 대상으로 4년 6개월 동안 만성간질환 발생을 파악하였다. 의료보험공단의 수진내역 및 해당 의무기록 조사, 암등록사업자료 및 통계청 사망원인통계자료와의 연계를 통하여 총 102명에서 간질환이 발생하였다. 대상 코호트를 간질환의 잠재적 발병자 집단은 기초조사 자료에서 B형간염 표면할원 양성인 경우, 혈청 간효소인 GPT(ALT)가 40 이상으로 증가된 경우, 또는 설문조사 결과 간질환을 앓았거나 현재 앓고 있는 대상자로 정의하였으며 총 2,217명이었다. Cox의 회귀분석모형으로 전체 코호트 대상자, 잠재적 발병자 집단 및 기타 대상자 7,305명에 대하여 각각 위험요인을 파악하였으며, 각 분석결과를 비교하였다. 전체 대상자 및 잠재적 발병자 집단에 대한 분석결과는 대체적으로 일치하며, 표면항원 양성, 높은 간효소치, 급성간염 과거력, 최근 금연, 금주 등이 유의한 위험요인이었으며, 식이 측면에서는 돼지고기, 커피 등이 보호요인으로 관찰되었다. 한편 잠재군에서는 비만도가 높을 경우 위험도가 감소하는 경향이 있었다. 잠재적 발병자 집단을 제외한 코호트 대상자 분석에서는 상이한 양상이 관찰되었는데 체질량지표의 경우 비만도가 높은 집단에서 발생 위험도가 4배 정도로 증가하였다(p<0.10). 그러나 금주나 금연 등은 무관하였으며, 생간(生肝) 섭취, 가공생선 및 된장찌개의 섭취 빈도가 위험도를 증가시켰다. 한편 표면항원 양성, 높은 간효소치, 급성간염 과거력 등은 다른 대상자의 분석결과가 유사하였다. 이상의 결과에서 잠재적 발병자 집단을 포함할 경우, 질병의 결과로 변화하는 생활습관이 해당 질병의 위험요인으로 파악될 가능성이 있었으며, 특히 금연, 금주 및 비만도 등이었다. 즉 연구자들은 만성적 경과를 밟아 진행되며 임상적으로 확인되는 질병에 대한 코호트 연구에서 잠재적 발병자 집단을 감안하지 않을 경우 발생하는 비뚤림의 가능성을 실증적으로 평가하였다. 4년 6개월간의 추적조사기간 동안 102명의 만성간질환 발병 예를 확인하였지만 잠재적 발병자 집단을 제외한 분석이 타당하며, 이들에서는 19명의 환자만이 발생하였다. 만성간질환의 위험요인에 대한 유용하고도 정밀한 연구결과를 도출하기 위해서는 향후 추적조사기간을 연장하여 충분한 발병 예를 확보할 필요가 있다. Objectives: The authors conducted the study to evaluate bias when potentially diseased subjects were included in cohort members while analyzing risk factors of chronic liver diseases. Methods: Total of 14,529 subjects were followed up for the incidence of liver diseases from January 1993 to June 1997. We have used databases of insurance company with medical records, cancer registry, and death certificate data to identify 102 incident cases. The cohort members were classified into potentially diseased group(n=2,217) when they were HBsAg positive, serum GPT levels higher than 40 units, or had or has liver diseases in baseline surveys. Cox's model were used for potentially diseased group, other members, and total subjects, respectively. Results: The risk factors profiles were similar for total and potentially diseased subjects: HBsAg positivity, history of acute liver disease, and recent quittance of smoking or drinking increased the risk. while intake of pork and coffee decreased it. For the potentially diseased, obesity showed marginally significant protective effect. Analysis of subjects excluding the potentially diseased showed distinct profiles: obesity increased the risk, while quitting smoking or drinking had no association. For these intake of raw liver or processed fish or soybean paste stew increased risk; HBsAg positivity, higher levels of liver enzymes and history of acute liver diseases increased the risk. Conclusions: The results suggested the potential bias in risk ratio estimates when potentially diseased subjects were included in cohort study on chronic liver diseases, especially for lifestyles possibly modified after disease onset. The analytic strategy excluding potentially diseased subjects was considered appropriate for identifying risk factors for chronic liver diseases.

수동형 원격탐지 FTIR 분광계의 Radiometric Calibration

김대성,박도현,최승기,나성웅,Kim, Dae-Sung,Park, Do-Hyun,Choi, Seung-Ki,Ra, Sung-Woong 한국광학회 2006 한국광학회지 Vol.17 No.5

본 논문에서는 온도 제어가 가능한 blackbody를 이용하여 수동형 FTIR 분광계의 radiometric calibration을 수행하고 타당성을 살펴보았다. Radiometric calibration은 분광계로 입사되는 radiance의 온도 변화에 대한 광 검출기의 파장 별 응답특성과 기기 내부에서 발생되는 온도 및 반사율에 의해 발생되는 측정 오차를 보정하여 Planck radiance로 변환하는 기법이다. 시료에 대한 calibration 과정을 수행한 스펙트럼을 spectral library와 비교한 결과, 흡수 스펙트럼의 파장 별 선폭과 상대적인 intensity가 매우 유사하게 나타났으며 입사 광원의 온도차와 비례하여 spectral intensity가 일정하게 증가됨을 확인할 수 있었다. In this paper, radiometric calibration of a FTIR spectrometer for passive remote sensing application was introduced and verified. Radiometric calibration is a significant signal processing procedure to retrieve the object radiance from the measured spectrum. The object radiance is measured and registered distorted by the detector's responsivity dependent on wavelength and instrument self-emission. Radiance of two temperature points, hot temperature and cold temperature, from a well-controlled blackbody was measured and used to obtain the scale factor and offset factor which are required for radiometric calibration. For gas phase C2H5OH. radiometric calibration was done and verified through comparison of its emission line width and intensity with the standard spectrum.

천연물 복합 추출물 (HAE-06)의 기관지 확장에 미치는 영향

김대성,김혜윰,Kim, Dae Sung,Kim, Hye Yoom 한국식품영양학회 2021 韓國食品營養學會誌 Vol.34 No.5

HAE-06 extract is a mixture of four medicinal plants, namely Lonicerae Folium et Caulis (Lonicera japonica), Scutellariae Radix (Scutellaria baicalensis), Adenophorae Radix (Adenophora triphylla var. japonica), and Polygonati Oddorati Rhizoma (Polygonatum odoratum var. pluriflorum). The HAE-06 extract demonstrated a concentration-dependent relaxing effect and enhanced cAMP production in bronchial smooth muscle that had been stimulated to contract with acetylcholine. Using a blocker, it was confirmed that the effect was through the β2-adrenergic receptor/cAMP/PKA pathway. In addition, it is thought that the HAE-06 extract has a bronchial smooth muscle relaxation effect by reducing the inflow of Ca²⁺ through the K⁺ and Ca²⁺ channels present in the sarcoplasmic membrane. If research continues in the future, it is believed that it will be possible to use it as a material for pharmaceuticals and functional foods.

골관절염 치료효과 및 안전성에 있어 S-Adenosylmethionine과 Ibuprofen의 비교 연구

김대성,정영옥,도주호,고혁재,백승인,김호연,이상헌,Kim, Dae-Sung,Jung, Young-Ok,Do, Ju-Ho,Koh, Hyek-Jae,Baek, Seung-In,Kim, Ho-Youn,Lee, Sang-Heon 대한임상약리학회 2003 Translational and Clinical Pharmacology Vol.11 No.2

Objective : To compare the efficacy and safety of S-adenosyl-methionine (SAMe) and ibuprofen in the treatment of patients with knee osteoarthritis. Methods : Sixty patients with knee osteoarthritis (OA) were enrolled in this study. Patients received a daily oral dose of 1200mg of SAMe and 1200mg of ibuprofen for 4 weeks Treatment efficacy was evaluated with clinical parameters as Lequesne's functional index and visual analog scale (VAS) of pain. Adverse effects were monitored from patient interviews and blood sampling. Results: The treatment efficacy evaluated by clinical parameters was not different in both groups. Gastrointestinal adverse effects were more frequently observed in ibuprofen group than in SAMe. Conclusion : SAMe was equally effective to ibuprofen in efficacy and better tolerated in short term treatment of patients with knee OA.

서울시 중년남성에서 육체적 활동량이 총 사망률에 미치는 영향에 관한 코호트 연구

김대성,구혜원,김동현,배종면,신명희,이무송,이충민,안윤옥,Kim, Dae-Sung,Koo, Hye-Won,Kim, Dong-Hyon,Bae, Jong-Myon,Shin, Myung-Hee,Lee, Moo-Song,Lee, Chung-Min,Ahn, Yoon-Ok 대한예방의학회 1998 예방의학회지 Vol.31 No.4

Although previous studies revealed the association of physical activity with mortality rate, it is unclear whether there is a linear trend between physical activity and mortality rate. In this study, the association of physical activity with the risk of all-cause mortality was analysed using Cox's proportional hazard model for a cohort of 14,204 healthy Korean men aged 40-59 years followed up for 4 years(Jan. 1993-Dec. 1996). Physical activity and other life style were surveyed by a postal questionnaire in December 1992. Total of 14,204 subjects were grouped into quartiles by physical activity. Using death certificate data, 123 deaths were identified. The second most active quartile had a lowest mortality .ate with relative risk of 0.44(95% C.I. : 0.23-0.84) compared with most sedentary quartile, showing a J-shape pattern of physical activity-mortality curve. By examining the difference in proportion of cause of the death between most active quartile and the other quartiles, there was no significant difference of proportional mortality from cardiovascular deaths, cerebrovascular deaths or deaths from trauma. The covariates were stratified into two group between which the trend of RR was compared to test the effect modification. There was no remarkable effect modification by alcohol intake, smoking, body mass index, calorie consumption, percent fat consumption. In conclusion, moderate activity was found to have more protective effect on all-cause mortality than vigorous activity and that the J-shape pattern of physical activity-mortality curve was not due to the difference of mortality pattern or effect modification by alcohol intake, smoking, body mass index, calorie consumption and percent fat consumption.

근골격계부담작업 유해요인조사 위해도 평가기준 가이드라인(위험수준별 모델) 개발

김대성,박정근,김규상,Kim, Dae-Seong,Park, Jeong-Geun,Kim, Gyu-Sang 대한산업보건협회 2008 산업보건 Vol.243 No.-