발효유에서의 단백질 분석 방법의 비교

이광희,추영란 대구보건대학 1994 대구보건대학 論文集 Vol.14 No.-

A study was presented of the masurement of protein in fermented milk with the Folin reagent and with coomassie brilliant blue G 250. Inoculated skim milk was estimated in pH and protein concentration each 3hrs. Protein concentrations by lowry method were shownd various change and by braford method lower gradually. It seems that the protein concentration in fremented milk are influenced strongly by protease activity. The adventage of braford method is the simplicity and rapidity, but coomassie blue do not react with free amino acid and very small peptide.

전문가에 대한 평가의 접근방법과 한계 : 한국연구재단 연구사업관리전문가(PM)를 중심으로

이광희 대한지방자치학회 2018 한국지방자치연구 Vol.20 No.1

본 연구는 연구관리 전문기관에서 도입 확산되고 있는 전문가(Program Manager) 평가방법과 접근에 대해 한국연구재단의 운영 사례를 통해 바람직한 전문가 평가의 발전방향을 모색하는데 있다. 한국연구재단의 전문가에 대한 평가는 첫째, 목적달성 평가모형과 과정평가 접근방법이 혼재된 유형이다. 둘째, 고객지향적 접근방법과 반응적 접근방법을 선택하고 있으며 전문가의 업무수행과정에 대해 조직 내․외부의 이해관계자의 기대와 수요를 얼마나 만족시키는가에 평가의 초점을 두고 있다. 셋째, 전문가에 대한 평가의 목적은 전문성과 공정성, 책임성을 점검하면서 그 평가 결과를 서열화 하고 있다. 넷째 한국연구재단은 전문가 개인의 역량평가에 초점을 두고 있다. 결론적으로 한국연구재단의 전문가 평가는 다 측면의 평가를 모두 포괄하려는 모형으로 볼 수 있으나, 전문가 평가의 필요성에 대한 검토, 전문가 평가의 측정 및 척도의 일관성 등에서 한계점이 있다. This study first review the evaluation method for the expert and then do the case study for the expert(PM, program manager) in national research foundation of korea(NRF). First, the evaluation on expert performed by NRF shows the mixed model of objective oriented evaluation and formative evaluation. Second, NRF chose consumer oriented approach and responsive approach. Then, it focuses on how to the extent the expert responses to the consumer. Third, the objective of the evaluation on expert assesses expertise, equity, and responsibility. Fourth, NRF emphasizes the evaluation of individual capability for the expert. Finally, the evaluation of expert performed by NRF shows the general evaluation model, trying to include all evaluation model and method. Also, it limits the study about necessity of expert evaluation and the consistency of evaluation method index.

학술발표회 : 번식 ( 구두발표 ) ; 돼지 난자 투명대의 단일클론 항체 생산 및 특성화

이광희,이홍준,최승철,이상호 한국동물자원과학회 ( 구 한국축산학회 ) 1995 한국축산학회 심포지움자료집 Vol.- No.-

재난안전 분야 평가제도 메타평가 및 개선방안

이광희,이환성 한국행정연구원 2017 기본연구과제 Vol.2017 No.-

$Na^+$$\longleftrightarrow$$Ag^+$ 이온교환에 따른 Na_2O-Al_2O_3-SiO_2$ 계 유리의 특성 및 살균효과

이광희,최세영 한국세라믹학회 1996 한국세라믹학회지 Vol.33 No.2

Glasses of Na2O-Al2O3-SiO2 system were prepared and ion-exchange characteristics change of properties and bactericidal effects by Na+↔Ag+ ion exchange were studied. Parent glasses with three compositions of varying Na2O in the 20~30 wt% were ion-exchanged in the molten salt of 2 mol% AgNO3+98 mol% NaNO3 at 320~36$0^{\circ}C$ for 15~16min. Amount of ion exchange and penetration depth of Ag+ ion increased with Na2O content in the parent glass ion exchange temperature and time. After ion exchange densities and Vickers hardness of the glasses increased and the glasses showed yellow-brown color and as amount of ion exchange increased the color turned deep because partial reduction and agglomeration of Ag+ into Ag0 results in absorption of visible light. After ion exchange chemical durability of the glass to wter was enhanced compara-bly that weight loss and change of surface of the glass were not found for the leaching test in 5$0^{\circ}C$ K.I water for 240hrs. Bactericidal effect of ion exchanged glass on Staphylococcus aureus and E. coli was determined by microorganism test and bactericidal effect increased with amount of ion exchange and incubation time.

고령환자의 대퇴경부 골절에 대한 양극성 고관절 반치환술 : 술전 기존질환이 술후 고관절 기능평가에 미치는 영향을 중심으로

이광희,김세동,이동철,신덕섭 대한골절학회 1998 대한골절학회지 Vol.11 No.1

The displaced femoral neck fracture remains a major challenge to orthopedic surgeons, as the elderly population is increasing. Elderly patients with femoral neck fractures often have other medical conditions, fragile bones, and poor compliance. Thus, for elderly patients who need early ambulation and funtional recovery, bipolar prosthesis is accepted as an appropriate treatment. We report a study of 58 cases of bipolar hemiarthroplasty with a femoral neck fracture and a physiologic age older than 65 years from January 1991 to May 1996. The purpose of this study is to evaluate the functional outcome of bipolar hemiarthroplasty and correlation between preoperative medical conditions and Harris hip score in femoral neck fractures in the elderly patients. The results obtained were as followings. 1. 90% of patients had uncontrolled medical conditions. Cardiovascular and pulmonary disease were main preexisting medical conditions. 2. The preoperative medical conditions were important determinants for the functional results after bipolar hemiarthroplasty. 3. Dislocated bipolar prosthesis needed open reduction or revision to total hip arthroplasty due to disassembly of the prosthesis caused by attempted closed reduction.

딸기 ${\beta}$-Galactosidase의 정제 및 생화학적 특성

이광희,윤경영,김광수,김남우,신승렬,Lee, Kwang-Hee,Yoon, Kyung-Young,Kim, Kwang-Soo,Kim, Nam-Woo,Shin, Seung-Ryeul 한국식품저장유통학회 2000 한국식품저장유통학회지 Vol.7 No.2

${\beta}$-Galactosidase was extracted and purified from strawberry. The purified ${\beta}$-Galactosidase from strawberry was investigated their physicochemical characteristics. ${\beta}$-Galactosidase was purified 25.74 fold from strawberry. The purification procedure include ammonium sulfate fraction, acetone powder treatment and gel and ion exchange chromatography. Yield of the enzyme purification was 18.11%. The purified enzyme has native molecular weight of 116,000 dalton. Vmax value and Km value of ${\beta}$-Galactosidase were 0.077 mM ONPG/ml/15mim and 1.75x10-2mM, respectively. The optimum temperature and pH of ${\beta}$-Galactosidase were 43$^{\circ}$C and pH 4.0, respectively. The ${\beta}$-Galactosidase activity was stable below 50$^{\circ}$C and at pH 4.0 to pH 6.0. Among the metal ions Ca and Mg were did not affect, whereas K, Cu and Zn show a little effect on the enzyme activity. The ${\beta}$-Galactosidase activities were inhibited by treatment with EDTA and SDS.

한국기독교대학 채플 현황 및 개선방안 연구

이광희 평택대학교 사회과학연구소 2001 사회과학연구 Vol.5 No.-

이온 플레이팅법으로 제조한 (Ti$_{1-x}$Cr$_{x}$)N 박막의 마모특성에 관한 연구

이광희,박찬홍,이정중 한국표면공학회 2001 한국표면공학회지 Vol.34 No.2

($Ti_{1-x}$ $Cr_{x}$ )N coatings were deposited by an ion-plating method in a reactor with two separate metal sources, Ti and Cr. Ti was evaporated using an electron beam, while Cr evaporation was carried out by resistant heating. The Ti and Cr concentrations in the coatings were controlled by the Ti and Cr evaporation ratio. The coating hardness increased with increasing the Cr content(x) and showed a maximum value of 6,000 HK at around x=0.8. The critical load of the coatings, measured by the scratch test, was around 30 N. The wear resistance properties of the ($Ti_{1-x}$$Cr_{ x}$)N coatings were evaluated using a CSEM pin-on-disk type tribometer. A Cr-steel ball as well as a SiC ball, which had hardness values of 590 HK and 2,600 HK respectively, were used as the pin. After the wear test, the surface morphology, roughness and the concentration of the coatings were investigated, with the main focus being on the effect of wear debris and the transferred layer on the wear behavior.

공정성요인의 역할차이에 대한 연구

이광희 한국생산성학회 2002 生産性論集 Vol.16 No.1

This study investigates whether job satisfaction is differently related to justice perceptions; distributive justice, interactional procedural justice, formal procedural justice. Hierarchical regression analyses revealed that all three types of organizational justice perception significantly predicted job satisfaction. In addition, each of three justice perceptions accounted for significant unique variance in employee job satisfaction. However, interactional procedural justice and distributive justice were more strongly related to job satisfaction with distributive justice having the strongest relationship of the three justice perceptions. More studies are needed to understand the role of three organizational justice perceptions in explaining of employee job satisfaction. The limitation of this study was discussed.