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청정콩나물 재배기술 - II. 관수간격 및 관수량이 콩나물 생육에 미치는 영향
김선림,송진,송정춘,황종진,허한순,Kim Sun-Lim,Song Jin,Song Jung-Choon,Hwang Jong-Jin,Hur Han-Sun 대한두채협회 2000 물만 먹고 자라요 Vol.10 No.-
This study was conducted to investigate the effect on the growth of soybean sprouts under control of various water supply, and to develop the culture methods for the production of clean soybean sprouts. The yield and characteristics of soybean sprouts was
α - Linolenic Acid가 농축된 들깨지방산 에스테르에 대한 참깨 Lignan 물질의 항산화효과
정보영(Bo-Young Jeong),류수노(Su-Noh Ryu),허한순(Han-Sun Hur) 한국식품영양과학회 1997 한국식품영양과학회지 Vol.26 No.6
수종의 참깨 lignan 물질의 항산화 효과를 들깨 유래의 HALA ethyl ester(ALA, 78.1%)의 저장안정성에 대하여 중량증가와 과산화물가 (POV)를 측정하므로써 연구되었다. 50℃ incubator에서 중량증가법에 의하여 각 시험구의 유도기간을 측정한 결과, 참깨 lignan 물질의 하나인 sesamol 처리구(0.01% 및 0.02%)가 10.2일로 가장 안정하였고, ether extracts 처리구 9.0일, BHT 처리구 7.6일의 순으로 안정하였다. 또한, 참깨 lignan 물질인 sesamin 및 sesangolin 처리구는 대조구의 경우와 거의 차이가 없었다. 각 시험구를 23℃ 암소에서 저장하였을 때, POV의 증가속도는 control≥sesangolin≥sesamin>BHT>ether extract>sesamol 처리구의 순이었으며, 이때 sesamol 및 ether extract 처리구는 저장 9주 후의 POV가 15.0meq/㎏ 이하였다. 또한, 각 시험구를 4℃의 저온에서 5개월간 저장하였을 때, 대조구에서 가장 높은 POV(6.89meq/㎏)를 나타낸 것으로 보아 HALA ethyl ester의 산화안정성이 저장온도에 의하여 크게 영향을 받는 것으로 생각되었다. 결론적으로, 참깨 lignan 물질인 sesamol과 들깨 ether extracts가 들깨유 유래의 HALA ethyl ester에 대하여 유용한 항산화제로서 인정되었다. Antioxidant effect of several antioxidative components on the high purity α-linolenic acid(HALA; ALA, 78.1%) ethyl ester concentrated from perilla oil were investigated by measuring weight-gains and peroxide value(POV) during storage at 50℃, 23℃ and 4℃. Amounts of antioxidant components were 0.2g/㎏ HALA ethyl ester for sesamin, sesangolin and butylated hydroxytolune(BHT), and 0.1g/㎏ for sesamol and 100g/㎏ for ether extracts from perilla seed. The oxidative stability of HALA ethyl ester was particularly increased by adding sesamol, ether extracts and BHT, but sesamin and sesangolin scarcely showed an antioxidant effect. POV on the HALA ethyl ester added sesamol and ether extract was less than 15.0meq/㎏ by 9 weeks of storage at 23℃. However, in the case of low temperature storage at 4℃, all the samples estimated showed less than 7.0meq/㎏ in POV by 5 months. Consequently, sesamol and ether extracts were recognized as available antioxidant components on the HALA ethyl ester from perilla oil.
호밀 染色體를 添加한 밀의 染色體 變異 및 作物學的 特性
Kwang Geun Park(朴光根),Wan Shik Ahn(安完植),Han Sun Hur(許翰淳) 한국육종학회 1989 한국육종학회지 Vol.21 No.2
This study was conducted to introduce the useful agronomic characteristics of rye into wheat. Primary triticale was obtained from a intergeneric hybridization between Triticum aestivum cv. Chugoku 81 and Secale cereale cv. Kyeonggi Jaerae. The somatic chromosome numbers of BC² F₁ populations which backcrossed with wheat two times to the primary triticale were ranged from 35 to 47. Rye chromosome di-addition wheat lines appeared the percentage of 3. 6 after the spontaneous selfing of mono-addition wheat lines and only 12. 7 percentages of these di-addition lines maintained same chromosome number to the next generation. The added rye chromosomes in pollen mother cell were easily eliminated during the meiosis. Rye chromosome addition wheat lines showed no distinct differences in morphology in comparison to wheat. It was considered that one rye chromosome, either mono or di-addtion, could not much effects to wheat phenotype.
들깨 葯培養에서 低溫 및 生長調整劑 處理가 캘러스誘導와 植物體 再分化에 미치는 影響
Hyo Sung Lee(李孝承),Jung Il Lee(李正日),Su Noh Ryu(柳守魯),Han Sun Hur(許翰淳) 한국육종학회 1994 한국육종학회지 Vol.26 No.4
This experiment was carried out to determine the effect of low temperature pre treatment and plant growth regulators on callus induction and plant regeneration in perilla anther culture. Perilla panicle with floral envelope was pretreated in low temperature at 5℃ and l0℃ for three days and compared with contral. MS basic medium supplemented with 2, 4-D 1~5㎎/ℓ, NAA 1~3㎎/ℓ and kinetin 2~5㎎/ℓ was used for callus induction and MS medium supplemented with NAA 0.1㎎/ℓ and BA 0.5㎎/ℓ was used for plant regeneration. Cold treated anthers at 5℃ for three days induced more callus and plant regeneration than others. In percentage of callus induction, MS medium supplemented with 2, 4-D ㎎/ℓ, NAA 1㎎/ℓ and kinetin 2㎎/ℓ induced more callus than N6 and B5 medium supplemented with the same concentration of plant growth regulators. The percentage of plant regeneration of Yeopsil×Jukjaso (F₁) in MS medium supplemented with 2, 4-D 5㎎/ℓ, NAA 3㎎/ℓ and kinetin 5㎎/ℓ was about 63%, while that in MS medium supplemented with 2, 4-D 2㎎/ℓ, NAA 1㎎/ℓ and kinetin 2㎎/ℓ was about 32%. The percentage of plant regeneration of suwon 32 showed the same tendency at the same condition in the case of 10℃ pretreatment for three days on Yeopsil×Jukjaso(F₁).
들깨유로부터 α - Linolenic Acid의 순수분리
정보영(Bo-Young Jeong),류수노(Su-Noh Ryu),허한순(Han-Sun Hur) 한국식품영양과학회 1997 한국식품영양과학회지 Vol.26 No.6
저온분별결정법과 질산은 함침 실리카 칼럼크로마토그래피를 이용하여 들깨유 지방산 혼합물로부터 고순도 ALA를 분리 정제한 결과를 보면 다음과 같다. 저온분별결정법에 의하여 순도 78% ALA(HALA) (회수율, 약 83%)의 대량농축이 가능하였으며, 이때의 동결조건은 -80℃에서 210분이었다. HALA ethyl ester를 대형 유리칼럼(100×10㎝, i.d.)를 이용한 질산은 함침실리카 칼럼크로마토그래피에 적용한 결과, 순도 90% 이상의 ALA ethyl ester(회수율, 71%)를 대량으로 얻을 수 있었다. Low-temperature crystallization method and silver nitrate-impregnated silicic acid column chromatography were applied for the isolation of pure α-linolenic acid(ALA) from perilla seed oil. ALA of 78% in purity(HALA; yield, 83%) was obtained from the fatty acid mixture(ALA, 65.7%) derived from perilla oil by the low-temperature crystallization method, when the mixture was frozen at-80℃ for 210min. ALA over 90% in purity(yield, 71%) was also obtained from HALA ethyl esters(ALA, 78%) by the silver nitrate-impregnated silicic acid column(100㎝×10㎝, i.d.) chromatography. In addition, the silver nitrate-impregnated silicic acid could be semipermanently used for isolation of ALA, because Ag^+ ion was not dissociated from the stationary phase.
박남규,김선림,허한순,박충현 한국국제농업개발학회 2002 韓國國際農業開發學會誌 Vol.14 No.1
재래종 지황과 신육성품종 지황 1호를 이용하여 절단 및 원형으로 숙지황을 제조한 결과는 다음과 같다. 1. 가압에 의한 숙지황 제조시간이 재래종은 254∼264시간, 제품수율이 71.42∼71.48%였고, 지황 1호는 제조시간이 260∼276시간 소요되었고, 수율은 71.50∼71.51%였으며 중량은 감소하는 경향이었다. 2. 상압에 의한 숙지황 제조에서도 가압에 의한 제조와 비슷한 경향을 보였다. 3. 가압과 상압에 의한 숙지황 제조 후 유리당 조성은 재래종, 지황 1호 전처리구에 63∼65% 이상이 fructose였으며, 30% 이상이 glucose였고, sucrose, maltose가 함유되었다. 4. 가압 및 상압에 의한 숙지황 제조시 catalpol 함량 변화는 종자회수가 많아질수록 caltalpol 함량이 낮아지는 경향이었으나 완전히 없어지지는 않았다. 5. 숙지황 제조 후 포장재별로 포장하여 상온에 저장한 결과 제품에 곰팡이 발생이 없어 저장에 문제가 없었다. New processing method for R. Radix preparata was developed by using the landrace variety as a control and "Jiwhang 1" (Rehmannia glutinosa Liboschitiz). Sliced Rehmanna glutionsa Liboschitiz was steam heated by normal and high pressure conditions for the round-shaped R. Radix preparata processing. About 254 to 264 hours were required for the processing of domestic variety, and about 260 to 280 hours were required for "Jiwhang 1", but their yields were ranged about 71.42∼71.6%. Fructose and glucose were major free sugar of R. Radix preparata, and their composition rates were 63∼65% and 30%, respectively, but sucrose and maltose were traced only small amount. The Catalpol content was decreased as increasing the frequencies of steam heating repetion. But the catalpol was not completely removed. After processing, R. Radix preparata packed with paper-bag or PE-bag, and storaged at common temperature was keeping a best quality.